- Tytuł:
- Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis
- Autorzy:
-
Grąz, Marcin
Rachwał, Kamila
Zan, Radosław
Jarosz-Wilkołazka, Anna - Powiązania:
- https://bibliotekanauki.pl/articles/1038790.pdf
- Data publikacji:
- 2016
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
oxalate oxidase
oxalic acid
Abortiporus biennis - Opis:
- Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of Km for oxalate and Vmax for the enzyme reaction were 0.015 M and 30 mmol min-1, respectively.
- Źródło:
-
Acta Biochimica Polonica; 2016, 63, 3; 595-600
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł