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    Wyświetlanie 1-5 z 5
    Tytuł:
    Funkcjonariusze Państwowej Straży Pożarnej w świetle badań socjologicznych
    Officers of the State Fire Service in the View of Sociological Research
    Autorzy:
    Stochmal, M
    Powiązania:
    https://bibliotekanauki.pl/articles/136761.pdf
    Data publikacji:
    2013
    Wydawca:
    Szkoła Główna Służby Pożarniczej
    Tematy:
    bezpieczeństwo
    Państwowa Straż Pożarna
    system bezpieczeństwa
    socjologia grup dyspozycyjnych
    wzór społeczny
    safety
    State Fire Service
    security system
    sociology of dispositional groups
    social pattern
    Opis:
    W artykule zaprezentowano wybrane socjologiczne aspekty badań empirycznych nad Państwową Strażą Pożarną, dokonanych w Instytucie Socjologii Uniwersytetu Wrocławskiego. Usytuowanie tych badań we wrocławskim ośrodku akademickim zbiegło się z jubileuszem XX-lecia Państwowej Straży Pożarnej (PSP). Główną osią rozważań zamieszczonych w artykule jest prezentacja wzoru społecznego funkcjonariusza PSP jako przedstawiciela paramilitarnej formacji systemu bezpieczeństwa. Jest to osoba podejmująca trud codziennej służby, zapewniając szeroko rozumiane bezpieczeństwo w przestrzeni metropolitalnej.
    This article presents selected empirical sociological aspects of the State Fire Service, carried out at the Institute of Sociology at the University of Wroclaw. The location of these studies in Wroclaw academic center coincided with the celebration of twenty anniversary of the State Fire Service (SFS) existence. The main axis of the considerations contained in the article is to present the social model of a firefighter as a representative of paramilitary formations of safety system. This is the person who takes the trouble of daily service providing safety understood as the safety the metropolitan area.
    Źródło:
    Zeszyty Naukowe SGSP / Szkoła Główna Służby Pożarniczej; 2013, 4, 48; 137-164
    0239-5223
    Pojawia się w:
    Zeszyty Naukowe SGSP / Szkoła Główna Służby Pożarniczej
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Pentacyclic triterpenoids and polyphenols accumulation in cell suspension culture of Chaenomeles japonica (Thunb.) Lindl. ex Spach
    Autorzy:
    Kikowska, M.
    Wlodarczyk, A.
    Stochmal, A.
    Zuchowski, J.
    Thiem, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/71498.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Włókien Naturalnych i Roślin Zielarskich
    Tematy:
    pentacyclic triterpenoid
    triterpenoid
    polyphenol
    accumulation
    cell suspension
    callus
    Chaenomeles japonica
    Japanese quince
    chlorogenic acid
    ursolic acid
    oleanolic acid
    Opis:
    Introduction: Callus and cell suspension cultures are widely applied in investigation of production of highvalue secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance. Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary metabolites – pentacyclic triterpenoids and polyphenols. Methods: To establish a cell suspension culture, stabilized and homogeneous callus was selected. Cell cultures were systematically passaged every 2 weeks to fresh liquid medium with the same composition. Biomass from cultures at the growth phase and stationary phase was designated for phytochemical research. UHPLC-DAD-MS analyzes were performed. At the same time, their macroscopic and microscopic observations were carried out. Results: Cells of suspension culture line A2 were characterized by the intense divisions. Cell culture extracts (both from the growth phase and stationary phase) contained pentacyclic triterpenoids. In addition, phenolic compounds (chlorogenic acid and proanthocyanidins type B) and in a small amount also epicatechin are present in the extract of the cells harvested from the growth phase. In the present studies, three pentacyclic triterpenoids were detected and quantified in the extracts of cell suspensions and callus line A2. Ursolic and oleanolic acids were the main triterpenoids in the studied extracts. The cell suspension culture from the growth phase exhibited the highest content of ursolic, oleanolic, and betulinic acid (separately and together). Conclusion: The cell suspension culture of Chaenomeles japonica is a promising source of pentacyclic triterpenoids.
    Źródło:
    Herba Polonica; 2019, 65, 1
    0018-0599
    Pojawia się w:
    Herba Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Eryngium campestre L. via shoot culture provides valuable uniform plant material with enhanced content of phenolic acids and antimicrobial activity
    Autorzy:
    Kikowska, M.
    Thiem, B.
    Sliwinska, E.
    Rewers, M.
    Kowalczyk, M.
    Stochmal, A.
    Dlugaszewska, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/19447.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro- and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg lˉ¹ benzyladenine (BA) and 0.1 mg lˉ¹ indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2016, 58, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Elicitation of anthocyanin production in roots of Kalanchoe blossfeldiana by methyl jasmonate
    Autorzy:
    Goraj-Koniarska, J.
    Stochmal, A.
    Oleszek, W.
    Moldoch, J.
    Saniewski, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/19687.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The influence of methyl jasmonate on anthocyanin accumulation in roots of Kalanchoe blossfediana plants was studied. Methyl jasmonate (JA-Me), at a concentration of 5.0 to 40.0 mg.l-1, substantially increased anthocyanin accumulation in roots of intact plants, when it was applied as a solution under natural light conditions. The production of anthocyanin depended on the concentration of methyl jasmonate and the age of the plant. The stimulatory effect was higher in older plants of K. blossfeldiana than in younger ones. When leaves were removed methyl jasmonate slightly stimulated anthocyanin accumulation compared with intact plants. The obtained results indicate that leaves are necessary for the anthocyanin accumulation in the roots. In isolated roots methyl jasmonate did not affect the accumulation of anthocyanins in light conditions. Seven anthocyanins were documented in the roots of control plants and 8 anthocyanins in the roots of JA-Me treated ones. JA-Me increased the level of anthocyanins in roots of old K. blossfeldiana plants 6.8, 6.0 and 3.6-folds, after 4, 8 and 14-days of treatment, respectively.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2015, 57, 1
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evaluation of antiamoebic and antimicrobial activities in vitro of Chaenomeles japonica (Thunb.) Lindl. ex Spach extracts
    Autorzy:
    Klikowska, M.
    Derda, M.
    Thiem, B.
    Wlodarczyk, A.
    Dlugaszewska, J.
    Stochmal, A.
    Zuchowski, J.
    Hadas, E.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117842.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Acanthamoeba spp.
    chaenomeles japonica
    pentacyclic terpenoids
    plant extracts
    plant
    in vitro cultures
    polyphenols
    staphylococcus aureus
    Opis:
    The free-living Acanthamoeba sp. causes various diseases. Treatment of them is very difficult and not always effective because of encystation, making it highly resistant to antiamoebic drugs. Gram-positive bacteria staphylococcus aureus, Gram-negative bacteria Escherichia coli, and an yeast candida albicans also exhibit outstanding resistance to antimicrobial substances. The search for new natural amoebicidal and antimicrobial agents of plant origin is still of current interest. The aim of the study was to investigate the amoebicidal activity of the extracts obtained from tissue culture and a field-grown plant of chaenomeles japonica against pathogenic trophozoites of Acanthamoeba spp. and antimicrobial effect against s. aureus, E. coli, and c. albicans. The extracts of c. japonica had an inhibitory effect on the proliferation of Acanthamoeba trophozoites as compared to the non-treated control. among the crude extracts tested, the extract of leaves, from both shoot culture and the field-grown plant had remarkable amoebicidal action against the trophozoites but also antibacterial activity against Gram-positive bacteria staphylococcus aureus. The extract from leaves from shoot culture, already on the second and third days of treatment, showed an antiamoebicidal effect at a concentration of 1 mg ml-1 (inhibi- tion of trophozoites 87.5% and 91.8%, respectively). In addition to leaves from shoot culture (a conc. 5 mg ml-1, 2nd day inhibition of trophozoites 85.7% and 3rd day 97.2%), leaves from a field-grown plant (a conc. 5 mg ml-1, 2nd day 91.0% and 3rd day 94.4%) and callus (a conc. 5 mg ml-1, 2nd day 90.0% and 3rd day – 95.4%) also exhib- ited a good antiamoebicidal activity. Out of the four extracts, the extracts from leaves from both shoot culture and a field-grown plant were reported to be the most active against Gram-positive s. aureus, which was determined by the values of MIC = 5.0 mg ml-1 and MIC = 2.5 mg ml-1, respectively. The inhibitory potential depends on the yield and composition of mainly bioactive compounds: pentacyclic terpenoids (mainly betulinic, ursolic, and oleanolic acids) and polyphenols (mainly chlorogenic acid and its isomers, epicatechin, dimeric, and trimeric proanthocyanidins, quercetin and kaempferol derivatives).
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 2; 47-58
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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