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    Wyświetlanie 1-4 z 4
    Tytuł:
    Improving growth indices and productivity of phytochemical compounds in lemon balm (Melissa officinalis L.) through induced polyploidy
    Autorzy:
    Talei, D.
    Fotokian, M.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2097129.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    colchicine
    flavonoid
    lemon balm
    physiological traits
    rosmarinic acid
    Opis:
    The induction of polyploidy using mutagenic chemicals is one of the plant breeding methods to enhance the production of secondary metabolites. In the current research, to induce polyploidy in lemon balm (Melissa officinalis) plants, seeds were treated with different concentrations of colchicine for various exposure times. A factorial experiment was performed using a randomized complete block design with two factors: colchicine concentrations with four levels (control, 0.05%, 0.1%, and 0.2%) and exposure times with three levels (24, 48, and 72 h) and three replicates. The physiological and phytochemical traits of plants were measured at a 4–6 leave stage. The results indicated that different concentrations of colchicine had a significant effect on the chlorophyll a (Chl.a), chlorophyll b (Chl.b), carotenoid, phenol, flavonoid, and rosmarinic acid contents. The exposure times of colchicine also caused significant changes in Chl.a, Chl.b, carotenoid, phenol, flavonoid, and rosmarinic acid amounts (P # 0.01). Increasing the colchicine concentration significantly increased the physiological and phytochemical traits at 0.05% and 0.1% concentration in comparison to the control (P # 0.01). In contrast, the interaction of colchicine concentration and exposure time had a significant effect on Chl.a, Chl.b, carotenoid, and rosmarinic acid amounts. The findings of this study indicate that one of the effective methods in primary screening of polyploidy plants in the polyploidization breeding program is the estimation of the physiological changes, the contents of chlorophyll a and b, and the total amount of chlorophyll and secondary metabolites. Flow cytometry is recommended to be used for the accurate identification of the ploidy level in M. officinalis.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 3; 215-226
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Methyl jasmonate, yeast extract and sucrose stimulate phenolic acids accumulation in Eryngium planum L. shoot cultures
    Autorzy:
    Kikowska, Małgorzata
    Kędziora, Izabela
    Krawczyk, Aldona
    Thiem, Barbara
    Powiązania:
    https://bibliotekanauki.pl/articles/1039089.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    chlorogenic acid
    elicitation
    flat sea holly
    in vitro shoot culture
    rosmarinic acid
    Opis:
    Eryngium planum L. has been reported as a medicinal plant used in traditional medicine in Europe. The tissue cultures may be an alternative source of the biomass rich in desired bioactive compounds. The purpose of this study was to investigate the influence of the biotechnological techniques on the selected phenolic acids accumulation in the agitated shoot cultures of E. planum. Qualitative and quantitative analyses of those compounds in 50% aqueous - methanolic extracts from the biomass were conducted by applying the HPLC method. Methyl jasmonate (MeJA), yeast extract (YE) and sucrose (Suc) stimulated accumulation of the phenolic acids: rosmarinic (RA), chlorogenic (CGA) and caffeic (CA) in in vitro shoot cultures. Cultivation of shoots in liquid MS media supplemented with 1.0 mg L-1 6-benzyladenine and 0.1 mg L-1 indole-3-acetic acid in the presence of 100 µM MeJA for 48h was an optimum condition of elicitation and resulted in approximately 4.5-fold increased content of RA + CGA + CA in plant material compared to the control (19.795 mg g-1 DW, 4.36 mg g-1 DW, respectively). The results provide the first evidence that the selected phenolic acids can be synthesized in elicited shoot cultures of flat sea holly in higher amount than in untreated shoots.
    Źródło:
    Acta Biochimica Polonica; 2015, 62, 2; 197-200
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In vitro cultures of Salvia officinalis L. as a source of antioxidant compounds
    Autorzy:
    Grzegorczyk, I
    Bilichowski, I.
    Mikiciuk-Olasik, E.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/57164.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Salvia officinalis
    carnosic acid
    concentration
    rosmarinic acid
    sage
    botany
    antioxidant compound
    in vitro
    carnosol
    Opis:
    The concentrations of carnosic acid, carnosol and rosmarinic acid in different materials from differentiated (multiple shoot cultures and regenerated plants) and undifferentiated (callus and cell suspension) in vitro cultures of Salvia officinalis were determined by HPLC. The results suggested that diterpenoid (carnosic acid and carnosol) production is closely related to shoot differentiation. The highest diterpenoid yield (11.4 mg g-1 for carnosic acid and 1.1 mg g-1 for carnosol) was achieved in shoots of 10-week-old micropropagated plants. The levels were comparable to those found in shoots of naturally growing plants. Undifferentiated callus and cell suspension cultures produced only very low amounts of carnosol (ca. 0.05 mg g-1 of dry weight). In contrast, content of rosmarinic acid in callus and suspension cultures as well as shoots growing in vitro and in vivo was similar and ranged between 11.2 and 18.6 mg g-1 of dry weight.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2005, 74, 1
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.
    Autorzy:
    Grzegorczyk, I
    Bilichowski, I.
    Mikiciuk-Olasik, E.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/56937.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    triacontanol
    shoot multiplication
    production
    antioxidant compound
    shoot culture
    Salvia officinalis
    carnosic acid
    carnosol
    rosmarinic acid
    Opis:
    This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in S. officinalis cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l-1 IAA, 0.45 mg l-1 BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l-1 was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l-1 increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2006, 75, 1; 11-15
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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