Temat: microsatellite DNA - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Polymorphism of microsatellite loci - a tool in studying biodiversity of paddlefish aquaculture broodstock
Autorzy:: Kaczmarczyk, D.
Kohlmann, K.
Kersten, P.
Luczynski, M.
Powiązania:: https://bibliotekanauki.pl/articles/363088.pdf
Data publikacji:: 2007
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: izolacja DNA
DNA mikrosatelitarne
amplifikacja PCR
DNA extraction
microsatellite DNA
PCR amplification
Opis:: American paddlefish (Polyodon spathula) is a new species in Polish aquaculture, its broodstocks are few and small, and it is possible that all mature fish originated from only a few spawners. Studies on polymorphism of highly variable microsatellite DNA allow revealing genetic characteristics of individual spawners as well as estimation of genetic variation within and divergence between broodstocks. This paper describes optimised protocols for isolation of DNA from fin tissues, amplification of nine microsatellite loci using PCR technique, and for fish genotyping using automatic capillary DNA sequencer. Our technique was tested towards the fin samples taken from all paddlefish reared in Poland and approaching their sexual maturity; the study included also samples taken from 47 fish of the Ukrainian breeding center (Gorny Tykich).
Źródło:: Environmental Biotechnology; 2007, 3, 2; 44-48
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Genetic identification of black caviar based on microsatellite DNA analysis
Autorzy:: Fopp-Bayat, D.
Powiązania:: https://bibliotekanauki.pl/articles/363094.pdf
Data publikacji:: 2007
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: identyfikacja genetyczna
czarny kawior
DNA mikrosatelitarne
genetic identification
black caviar
microsatellite DNA
Opis:: Sturgeons (Acipenseridae) are producers of black caviar, which is sold world-wide. Black caviar differs widely in quality, availability, price and taste and for these reasons it is often the subject of commercial fraud. Identification of sturgeon species is frequently based on molecular methods such as analysis of nuclear DNA and mitochondrial DNA. In this study two fragments of microsatellite DNA: Afu-39 and Afu-68 were analyzed. In caviar samples, four alleles were observed in locus Afu-39, and six alleles in locus Afu-68. This paper describes the use of microsatellite DNA markers for species identification of black caviar.
Źródło:: Environmental Biotechnology; 2007, 3, 2; 57-60
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: New multiplex PCR assays for estimating genetic diversity in rainbow trout (Oncorhynchus mykiss) by polymorphism of microsatellite DNA
Autorzy:: Kaczmarczyk, D.
Kaczor, A.
Powiązania:: https://bibliotekanauki.pl/articles/363262.pdf
Data publikacji:: 2013
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: microsatellite DNA
multiplex PCR
population genetics
rainbow trout
DNA mikrosatelitarne
genetyka populacyjna
pstrąg tęczowy
Opis:: Multiplex PCR is a useful technique for estimating genetic diversity. This paper presents 3 new sets of primer pairs for effectively amplifying 10 microsatellite DNA loci from rainbow trout (Oncorhynchus mykiss). Unlike other sets of primer pairs that have been developed for amplifying rainbow trout microsatellite loci, ours do not require the hot-start PCR technique. In the paper, we describe the steps taken to choose the loci for each multiplex assay and to verify the genotyping results. We provide the compositions of the PCR mixture and the characteristics of the PCR thermal profile recommended for amplification.
Źródło:: Environmental Biotechnology; 2013, 9, 1; 19-24
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Microsatellite DNA polymorphism in sturgeon species and their hybrids reared in Polish aquaculture farms
Autorzy:: Fopp-Bayat, D.
Luczynski, M.
Powiązania:: https://bibliotekanauki.pl/articles/363194.pdf
Data publikacji:: 2006
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: jesiotr
identyfikacja gatunkowa
DNA mikrosatelitarne
hybrydy
polimorfizm
hybrids
microsatellite DNA
polymorphism
PCR
species identification
sturgeons
Opis:: Highly variable microsatellite DNA loci show high levels of variation per locus and provide molecular markers for species and for populations of small effective size. In this study we applied microsatellite markers to identify specimens belonging to several sturgeon species and their interspecific hybrids. Nine microsatellite loci (Afu-19, Afu-22, Afu-34, Afu-39, Afu-54, Afu-57, Afu-58, Afu-68, Afu-69) were analysed for five species (beluga, Siberian sturgeon, Russian sturgeon, sterlet and paddlefish) and for their three interspecific hybrids: Siberian sturgeon x Russian sturgeon, beluga x sterlet (called bester) and for beluga x bester. Certain alleles at five loci: Afu-22, Afu-39, Afu-54, Afu-57 and Afu-68 were diagnostic for the examined sturgeon species and their interspecific hybrids.
Źródło:: Environmental Biotechnology; 2006, 2, 1; 11-19
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Tracing the genetic origin of brown trout (Salmo trutta) re-colonizing the Ecker reservoir in the Hartz National Park, Germany
Autorzy:: Kohlmann, K.
Wüstemann, O.
Powiązania:: https://bibliotekanauki.pl/articles/363212.pdf
Data publikacji:: 2012
Wydawca:: Uniwersytet Warmińsko-Mazurski w Olsztynie
Tematy:: pstrąg potokowy
Salmo trutta
mikrosatelitarne DNA
DNA
PCR
brown trout
microsatellite
Opis:: The Ecker reservoir and its main tributary had been free of brown trout (Salmo trutta) for several decades due to cumulative effects of natural and anthropogenic acidification. However, after the decline of emissions in the 1990s and the resulting rise of water pH to suitable for brown trout values, the species began to recolonize its original habitats. In the main tributary first brown trout individuals were caught in 2008 and in the reservoir in later years as well. Stocking could be excluded in both areas. Therefore, the present study was aimed to trace the genetic origin of these brown trout by genotyping eight microsatellite loci in samples collected in the reservoir, its main tributary, potential refugia and - for comparison - from two areas downstream of the dam being physically isolated for about 70 years. Genetic variability within populations (average number of alleles per locus), genetic differentiation between populations (FST values and genetic distances), occurrence of certain alleles and results of assignment tests indicated that the Ecker reservoir was re-colonized from two sources: the Große Peseke, a small direct inflow into the reservoir, and the Fuhler Lohnsbach, a parallel flowing brook connected to the reservoir by a pipe. Genetic data also supported recolonization of the main tributary from the reservoir but not in the opposite direction. Moreover, bottleneck effects were evident in brown trout populations upstream of the dam compared to the two populations downstream of the dam.
Źródło:: Environmental Biotechnology; 2012, 8, 2; 39-44
1734-4964
Pojawia się w:: Environmental Biotechnology
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Analysis of microsatellite instability and loss of heterozygosity in breast cancer with the use of a well characterized multiplex system.
Autorzy:: Powierska-Czarny, Jolanta
Miścicka-Śliwka, Danuta
Czarny, Jakub
Grzybowski, Tomasz
Woźniak, Marcin
Drewa, Gerard
Czechowicz, Włodzimierz
Sir, Jan
Powiązania:: https://bibliotekanauki.pl/articles/1043412.pdf
Data publikacji:: 2003
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: microsatellite instability
short tandem repeats
multiplex DNA typing
breast cancer
Opis:: Analysis of microsatellite instability (MI) and loss of heterozygosity (LOH) is recommended for screening patients with sporadic and hereditary malignancies. This study shows an application of a fluorescent hexaplex PCR system for microsatellite typing on A.L.F. DNA Sequencer (Pharmacia Biotech). This technique detects changes in microsatellites providing a time-efficient, reliable and accurate method for MI and LOH analyses. The Fragment Manager software was used for automated size calculation and quantitation of DNA fragments, enabling rapid and precise measurement of allelic ratios. We examined 70 breast cancer and 70 control DNA specimens, classified all the patterns of microsatellite alterations, and set up MI and LOH assessment criteria for the automated multiplex fluorescent method.
Źródło:: Acta Biochimica Polonica; 2003, 50, 4; 1195-1203
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Mitochondrial and nuclear DNA differentiation of Picea abies populations in Poland
Autorzy:: Nowakowska, J A
Powiązania:: https://bibliotekanauki.pl/articles/40976.pdf
Data publikacji:: 2009
Wydawca:: Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:: international conference
Europe
forest ecosystem
plant breeding
tree
Norway spruce
Picea abies
Polska
mitochondrial DNA
nuclear DNA
plant population
microsatellite marker
post-glacial migration
Opis:: The natural stands of Norway spruce in Poland are split between the southern and the northeastern parts of the country. Two so-called "spruceless" zones separate the northern spruce locations from those in the south, one "spruceless" zone is situated in Central Poland, and the other one in the Beskid Mts. Mitochondrial (STS) and nuclear (SSR) markers were used to perform the genetic identification of Norway spruce. Four different variants of haplotypes, "a", "b", "c" and "d", were found to occur in the nad1 locus of STS markers. Populations from the northern range of Picea abies distribution in Poland harboured exclusively haplotypes "c" and "d", except for the Białowieża population which had haplotypes "a" and "c". Populations from the "spruceless" zones contained four types of haplotypes whilst those from southern Poland were mostly composed of haplotype "a". High mean gene diversity was observed for both STS and SSR markers (HT = 0.529, and HT = 0.851, respectively). The total genetic differentiation of Norway spruce populations was very low (FST= 0.088). Two main groups of populations were distinguished in the dendrogram defined by Nei's genetic distances based on microsatellite markers. The distribution of the genotypes was scattered and did not show any connection with the spatial distribution of P. abies in Poland. Only the mtDNA markers were able to differentiate the northern populations of Norway spruce from the southern ones, proving the historical separation between the Baltico-Nordic and the Hercyno-Carpathian ranges of P. abies in Poland. By contrast, the microsatellite data suggested an overlap between the genotypes due to the human manipulation of Norway spruce stands in the past.
Źródło:: Dendrobiology; 2009, 61 Supplement
1641-1307
Pojawia się w:: Dendrobiology
Dostawca treści:: Biblioteka Nauki