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Wyświetlanie 1-15 z 46
Tytuł:
The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
Autorzy:
Sroka, J
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/51137.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
tick
Ixodes ricinus
Toxoplasma gondii
Borrelia burgdorferi
occurrence
polymerase chain reaction
genotyping
B1 gene
human population
infection
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Knowledge, protection behaviours and seroprevalence of Lyme borreliosis in inhabitants of Lublin Province, eastern Poland – evaluation of a prophylaxis programme
Autorzy:
Zając, V.
Sroka, J.
Wójcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/28763061.pdf
Data publikacji:
2023
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2023, 30, 3; 413-424
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources
Autorzy:
Stojek, N.M.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50046.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods
Autorzy:
Wojcik-Fatla, A.
Stojek, N.M.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50884.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
The aim of the present study was: – to compare methods for concentration and isolation of Legionella DNA from water; – to examine the efficacy of various modifications of PCR test (PCR, semi-nested PCR, and real-time PCR) for the detection of known numbers of Legionella pneumophila in water samples artificially contaminated with the strain of this bacterium and in randomly selected samples of environmental water, in parallel with examination by culture. It was found that filtration is much more effective than centrifugation for the concentration of DNA in water samples, and that the Qiamp DNA Mini-Kit is the most efficient for isolation of Legionella DNA from water. The semi-nested PCR and real-time PCR proved to be the most sensitive methods for detection of Legionella DNA in water samples. Both PCR modifications showed a high correlation with recovery of Legionella by culture (p<0.01), while no correlation occurred between the results of one-stage PCR and culture (p>0.1).
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland
Autorzy:
Cisak, E.
Zajac, V.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51019.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Repellents and acaricides as personal protection measures in the prevention of tick-borne diseases
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50494.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA was not detected in Dermacentor reticulatus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51730.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Opis:
A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Coincidence of three pathogens [Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti] in Ixodes ricinus ticks in the Lublin macroregion
Autorzy:
Wojcik-Fatla, A
Szymanska, J.
Wdowiak, L.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51180.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick
Ixodes ricinus
coincidence
pathogen
Borrelia burgdorferi
Anaplasma phagocytophilum
Babesia microti
Lublin macroregion
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 1; 151-158
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/52094.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces
Autorzy:
Sroka, J.
Karamon, J.
Dutkiewicz, J.
Wójcik-Fatla, A.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2081971.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
flotation
Toxoplasma gondii
faeces
Real time PCR
nested PCR
cats
oocysts detection
Opis:
Introduction and objective. The aim of the study was to compare the effectiveness of selected oocysts concentration methods, DNA extraction protocols and PCR assays targeting the B1 gene, for the development of procedures which would be effective and useful in laboratory practice for the detection of T. gondii in faecal samples from cats. Materials and method. In order to compare the influence of the flotation fluids on microscopy and PCR detection of T. gondii, saturated solutions of saccharose, MgSO4, ZnSO4 and NaNO3 were used. To determine the sensitivity of PCR tests used: Real time PCR (RT) and nested PCR, water samples spiked with T. gondii tachyzoites and oocysts were tested. DNA was extracted using DNeasy Blood and Tissue Kit (Qiagen) (K1). The same PCR tests were used to assess the efficacy of T. gondii DNA detection in samples of cat faeces spiked with oocysts, using DNA extraction by a K1 set and a K2 set (QIamp DNA Stool Mini Kit, (Qiagen). Results. The initial results showed that NaNO3 was most useful as a flotation fluid due to the lack negative effect on the oocysts and amplification efficacy in PCR. The level of detection for water samples (100 μl) was determined as 100 tachyzoites and 1–50 oocysts in RT, and 2–20 oocysts in nested PCR. The limit of detection (LD) for stool samples (250 mg) spiked with oocysts, where the K1 set was used, determined as 250 and 5 oocysts in RT and nested PCR, respectively. For samples extracted with the K2 set, LD in RT was determined as 1–50 oocysts (depending on the variant) and 50 oocysts in nested PCR. Conclusions. The most effective methods for detection of T. gondii in cat faeces seem to be centrifugal flotation with NaNO3, followed by DNA extraction with removing of inhibitors (K2 set) and Real Time PCR targeting B1 gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 680-685
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland
Autorzy:
Buczek, A
Rudek, A.
Bartosik, K.
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/49580.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lyme borreliosis
borreliosis
serology
ELISA test
forestry
worker
Polska
forestry worker
tick-borne disease
diagnosis
antibody
treatment
Borrelia burgdorferi
tick
immunoglobulin
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 257-261
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of fruit on the extracellular enzyme profiles of fungi
Autorzy:
Farian, E.
Cholewa, G.
Cholewa, A.
Matczuk, M.
Wójcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/28762964.pdf
Data publikacji:
2020
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2020, 27, 4; 562-567
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fungal diversity of root vegetables and soil rhizosphere collected from organic and conventional farms in Eastern Poland
Autorzy:
Kłapeć, T.
Cholewa, G.
Cholewa, A.
Dutkiewicz, J.
Wójcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/2081696.pdf
Data publikacji:
2018
Wydawca:
Instytut Medycyny Wsi
Tematy:
filamentous fungi
vegetables
rhizosphere soil
organic and conventional farms
Opis:
Objective. Determination of the concentration and species composition of filamentous fungi in root vegetables (carrots and red beetroots), and the adjacent rhizosphere soil, collected on organic and conventional farms in Eastern Poland. Materials and method. During the period 2015–2016, a total number of 80 samples of vegetables and 40 samples of soil were examined. From each type of farm, 20 samples of vegetables and 20 samples of the adjacent soil were examined. In addition, the study included 20 samples of vegetables from organic farms and 20 samples of vegetables from conventional farms purchased on the markets in the city of Lublin in Eastern Poland. In order to determine the concentration and species composition of filamentous fungi, both in vegetables and soil, the method of plate dilutions on Malt Agar medium (Difco) with chloramphenicol was used. The study was conducted in two parallel repetitions. Inoculated media were incubated at the temperature of 30° C for 72 hours, then at room temperature for 72 hours. The species composition of fungal flora was determined using macroscopic and microscopic methods, with the help of keys and atlases. Results. It was found that the mean concentration of fungi was higher in vegetables and soil from conventional farms than in those from organic farms. In the case of carrots and soil from conventional farms, this concentration was 4.93 and 5.10 log10 CFU g-1, respectively, whereas from organic farms – respectively, 3.81 and 4.20 log10 CFU g-1. In the case of beetroots and soil from conventional farms, the mean concentrations were also higher compared to organic farms – 5.09 vs. 3.93 and 4.95 vs. 4.23 log10 CFU g-1. In the examined vegetables and rhizosphere soil, 61 species of filamentous fungi were found, of which 12 belonged to the genus Penicillium, 4 to the genus Fusarium, and 2 species each to the genera: Aspergillus, Cladosporium and Trichoderma. As many as 28 (45.9%) fungal species that occurred in vegetables and soil are regarded as pathogenic for humans.
Źródło:
Annals of Agricultural and Environmental Medicine; 2018, 25, 2; 374-381
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological agents as occupational hazards - selected issues
Autorzy:
Dutkiewicz, J.
Cisak, E.
Sroka, J.
Wojcik-Fatla, A.
Zajac, V.
Powiązania:
https://bibliotekanauki.pl/articles/50867.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
biological agent
occupational hazard
health hazard
bioaerosol
allergy
human disease
toxin
virus
bacteria
fungi
infectious disease
zoonosis
tick-borne disease
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 46

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