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Tytuł:
Biomedical Applications of Synchrotron X-Ray Fluorescence
Autorzy:
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/1931695.pdf
Data publikacji:
1994-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
07.85.+n
87.59.-e
32.30.Rj
Opis:
Since the exact role of trace elements in living organisms is still not very well known, it is a need of a careful analysis and study on trace element distributions in various biomedical materials. The conventional techniques such as X-ray fluorescence, proton-induced X-ray emission, atomic absorption spectrophotometry do not always give precise determination, especially in microstructures areas. Since synchrotron radiation is very powerful and gives the opportunity to work with micro-beams the synchrotron radiation induced X-ray emission technique has been recently developed. Due to many properties of synchrotron radiation it becomes a very useful tool in science. In this paper a characterization of different apparatus for a trace element analysis as well as several applications are described to show the usefulness of the synchrotron radiation induced X-ray emission.
Źródło:
Acta Physica Polonica A; 1994, 86, 5; 695-703
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Pch1 eyespot resistance gene in the collection of wheat lines (Triticum aestivum L.)
Autorzy:
Kwiatek, M.
Pankiewicz, K
Wisniewska, H.
Korbas, M.
Danielewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/65147.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Endopeptidase marker EpD1b and STS marker XustSSR2001-7DL are closely linked to very effective eyespot resistance gene Pch1. Because of this, the aim of this study was to compare the results obtained under lab conditions using such markers with the results obtained under field conditions. 134 wheat breeding lines and Triticum aestivum L. var. Randevous used as a eyespot resistance control were analized. The combination of three methods allowed to select eight completely resistant or high resistant lines, that could be used in following breeding processes. Results obtained using endopeptidase and STS markers in 100% correlate with the phenotyping scoring.
Źródło:
Journal of Plant Protection Research; 2012, 52, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?
Autorzy:
Kwiatek, M.
Wisniewska, H.
Korbas, M.
Gawlowska, M.
Belter, J.
Majka, M.
Danielewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/66314.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
eyespot
resistance gene
wheat
winter wheat
breeding line
yield
inoculation
isoenzyme
molecular marker
plant resistance
Triticum aestivum
Opis:
Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Pch3 gene, originating from Dasypyrum villosum; and (4) the Q.Pch.jic-5A gene, a quantitative trait locus (QTL) located on chromosome 5A of Capelle Desprez. However, those loci have drawbacks, such as linkage of Pch1 with deleterious traits and limited effectiveness of Pch2 against the disease. Here we present an initial study which aims to characterize wheat pre-registration breeding lines carrying 12 eyespot resistance genes, consider their resistance expression in inoculation tests and the influence of resistance genotypes on the yield. We selected four groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A gene alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines. For the first time, the effect of the combination of Pch1 and Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.
Źródło:
Journal of Plant Protection Research; 2016, 56, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Eyespot resistance of winter wheat breeding lines evaluated with marker-assisted selection and inoculation tests at the seedling and adult plant stages
Autorzy:
Majka, M.
Kwiatek, M.
Korbas, M.
Danielewicz, J.
Gawlowska, M.
Goral, T.
Wisniewska, H.
Powiązania:
https://bibliotekanauki.pl/articles/65926.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant disease
fungal disease
eyespot
wheat
Triticum aestivum
resistance
winter wheat
plant breeding
breeding line
marker-assisted selection
inoculation
seedling
adult plant
Oculimacula yallundae
Oculimacula acuformis
Opis:
Eyespot is one of the most important fungal diseases of the stem base of wheat (Triticum aestivum L.). The presented study clearly demonstrated that the Pch1 gene was the main effective source for reducing the eyespot disease score in the analyzed winter wheat lines. Nevertheless, Pch1 was present only in 8−9% of the investigated lines. Using an isoenzymatic marker and molecular markers, the presence of the Pch1 gene and lack of the Pch2 gene was identified in six lines. Two lines, SMH 9409 and DL 358/13/4, were polymorphic in an isoenzymatic marker study. In the remaining three lines, C 3373/11-1, KBH 15.15 and KBP 1416, the Pch1 gene was identified only with the use of an isoenzymatic marker. Both genes Pch1 and Pch2, as well as the resistant variety Rendezvous, were found in three lines: DD 248/12, KBP 15.2 and STH 4431. In line DD 708/13, the presence of the Pch1 and Pch2 genes was identified, where the association between the Pch1 and the locus of the Xorw5 marker was broken. It was shown that the presence or absence of Pch1 and Pch2 genes did not significantly affect the grain yield (from the plot), although the yield was highest in the presence of both genes. A significant effect of the presence of the Pch1 gene on thousand kernel weight (TKW) was observed. Lines with the Pch1 gene showed significantly higher TKW values than lines without both genes or with the Pch2 gene only.
Źródło:
Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
X-Ray Absorption Near Edge Structure and Mössbauer Spectroscopy in Study of Iron Valence States in Tissues
Autorzy:
Dziedzic-Kocurek, K.
Banaś, A.
Kwiatek, W. M.
Stanek, J.
Powiązania:
https://bibliotekanauki.pl/articles/2044673.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
61.18.Fs
61.10.Ht
Opis:
X-ray absorption near edge structure Fe K-edge spectra and $\text{}^{57}$Fe Mössbauer spectra of selected standard compounds were recorded at room temperature. Valence and spin states of Fe in these samples known from Mössbauer spectroscopy were correlated with the shapes of X-ray absorption near edge structure spectra in search of possible application of X-ray absorption near edge structure spectroscopy as analytical tool determining local electronic states of iron in tissues. As an example, the X-ray absorption near edge structure spectra of healthy and cancerous tissues of prostate are shown, suggesting Fe$\text{}^{3+}$ in cancerous tissues.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 341-345
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Elemental Mapping of Prostate Tissue by Micro-SRIXE
Autorzy:
Banaś, A.
Banaś, K.
Falkenberg, G.
Dyduch, G.
Kwiatek, W. M.
Powiązania:
https://bibliotekanauki.pl/articles/2044669.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
Synchrotron radiation-induced X-ray emission is now a proven analytical method for the determination of trace elements. Better insight of the role of trace elements in tissue samples can be gained by acquiring complete distribution maps of these elements. Our approach is based on the acquisition of digital maps. In this contribution we present two different methods for obtaining normalization matrix: first - based on argon intensity in the single spectrum and second - based on changes of beam current intensity. The region for the scan was carefully chosen according to the histological view of the sample. A total area was scanned with a step size of 15 μm in each direction and measuring time of 30 s per pixel. The thickness of the samples was 15 μm. A polycapillary was used for beam focusing. At the energy of 18 keV the beam size on the sample was approximately 15 μm which is of the order of a cell diameter.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 323-328
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Preliminary Study on Chemical Speciation of Sulphur in Cancerous Tissues
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Kisiel, A.
Cinque, G.
Falkenberg, G.
Powiązania:
https://bibliotekanauki.pl/articles/2044744.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.Gb
Opis:
Sulphur is vital for almost all living organisms by participating in a wide variety of metabolic processes. Nevertheless its biochemistry is only partially understood, due to it a few tools such as X-ray absorption near edge structure may be used to determine its chemical speciation in biological system. In this paper we focused on analysis of composition and elemental distribution in tissue structures of biological samples. We examined cancerous and non-cancerous prostate tissues. Experiments were carried out on the L-beam line at the HASYLAB, DESY (Hamburg, Germany) and DAΦNE-Light Facility at the Laboratori Nationali di Frascati (Frascati, Italy). Synchrotron radiation induced X-ray emission measurements reveal the significant differences in concentration of sulphur between cancerous and non-cancerous tissues. X-ray absorption near edge structure spectra show two main types of sulphur that are represented by X-ray peaks at 2476 eV and 2484 eV. This suggests that different sulphur components are present in the measured samples.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 383-387
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distinguishing Prostate Cancer from Hyperplasia
Autorzy:
Kwiatek, W. M.
Banaś, A.
Banaś, K.
Podgórczyk, M.
Dyduch, G.
Falkenberg, G.
Gajda, M.
Cichocki, T.
Powiązania:
https://bibliotekanauki.pl/articles/2044731.pdf
Data publikacji:
2006-03
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.-t
Opis:
The prostate gland is the most common site of neoplastic disorders in men. The knowledge of the pathogenesis of benign prostate hyperplasia and prostate cancer is still under investigation. X-ray fluorescence analysis using synchrotron radiation seems to be an appropriate technique to study the elemental composition of biological tissues. The measurements presented in this paper were carried out on the L-beam line at the HASYLAB, DESY (Germany). The observed concentrations of Fe, Cu, Mn, and Zn are significantly higher in cancerous tissues, as compared to normal and hyperplastic ones. Our results reveal also the existence of the two types of hyperplasia.
Źródło:
Acta Physica Polonica A; 2006, 109, 3; 377-381
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Neurogenic heterotopic ossifi cation – case study
Neurogenne skostnienia heterotopowe – studium przypadku
Autorzy:
Rolka, Łukasz
Browiński, Daniel
Kwiatek-Rolka, Karolina
Sielska, Małgorzata
Sielski, Grzegorz
Nyka, Walenty M.
Powiązania:
https://bibliotekanauki.pl/articles/1798663.pdf
Data publikacji:
2016
Wydawca:
Akademia Wychowania Fizycznego im. Bronisława Czecha w Krakowie
Tematy:
neurogenic heterotopic ossifi cation
physical therapy
electric shock
prophylaxis
neurogenne skostnienia heterotopowe
fi zjoterapia
porażenie prądem elektrycznym
profi laktyka
Opis:
The authors presented the case of a 30-year-old man in whom sudden cardiac arrest occurred as a result of high voltage electric shock. Starting from the 2nd week after the accident, rehabilitation was carried out in hospital conditions, designed to maintain range of motion in the joints, and from the 6th week, intensive rehabilitation was performed at the patient’s home. Despite the implemented treatment, total mobility restriction was observed in the hip joints, and based on spatial projection radiography and a CT, the patient was diagnosed with massive neurogenic heterotopic ossifi cation (NHO). Two surgeries were performed to remove the NHO: fi rst, from the left area (15th month after the accident), and then the right hip joint (18th month following the accident). After the intervention there was a signifi cant increase in mobility of both hips and a decrease in pain, which resulted in signifi cantly improved functional capabilities of the patient. In addition, prophylaxis to prevent the recurrence of NHO was implemented in order to maintain both passive and active range of motion, and the use of physical therapy treatments in the form of deep oscillation were performed. The results of the CT conducted in the 41st month following the accident revealed lesser NHO than the originally diagnosed. Rolka Ł., Browiński D., Kwiatek-Rolka K., Sielska M., Sielski G., Nyka W.M. Neurogenic heterotopic ossification – case study. Med Rehabil 2016; 20(4): 22-27. DOI: 10.5604/01.3001.0009.5482
Obrzęki kończyn dolnych mogą być wynikiem nieprawidłowości budowy i funkcji układu chłonnego, urazów, zapalenia, mogą się też wiązać z chorobą nowotworową lub jej leczeniem, jednak coraz częściej są jednym z podstawowych objawów towarzyszących kobietom w III trymestrze ciąży, głównie na skutek zaburzeń w układzie żylnym. Celem pracy jest przedstawienie obecnego stanu wiedzy na temat czynników ryzyka, profilaktyki i leczenia obrzęków kończyn dolnych u kobiet w ciąży. Wśród czynników ryzyka obrzęku związanego z ciążą wymienia się: wzrost objętości krwi krążącej, powiększenie macicy, wzrost masy ciała oraz zmiany w gospodarce hormonalnej. Zaburzenia żylne powstają w wyniku nadciśnienia żylnego na skutek niewydolności pompy mięśniowej oraz niewydolności zastawek żylnych. Ciąża, wykonanie cięcia cesarskiego, okres połogu sprzyjają wystąpieniu zakrzepicy żylnej. Podstawową i niekwestionowaną metodą stosowaną w profilaktyce i leczeniu zaburzeń układu żylno-limfatycznego oraz ich powikłań jest kompresjoterapia, wykorzystująca bandażowanie kompresyjne oraz stosowanie produktów uciskowych. Ucisk może być stosowany samodzielnie lub w połączeniu z innymi metodami, np. z manualnym drenażem limfatycznym, przerywaną kompresją pneumatyczną, z ćwiczeniami fizycznymi i oddechowymi. Na podstawie badań naukowych oraz w oparciu o rekomendacje ekspertów, ucisk wydaje się też skutecznym rozwiązaniem w profilaktyce i leczeniu zakrzepicy żylnej i obrzęku kończyn dolnych u kobiet w ciąży, jednak wymaga dalszych badań zgodnych z zasadami evidence based medicine.
Źródło:
Medical Rehabilitation; 2016, 20(4); 22-27
1427-9622
1896-3250
Pojawia się w:
Medical Rehabilitation
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular diversity of Clostridium botulinum and phenotypically similar strains
Autorzy:
Grenda, T.
Kukier, E.
Sieradzki, Z.
Goldsztejn, M.
Kwiatek, K.
Powiązania:
https://bibliotekanauki.pl/articles/31310.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This study was undertaken to examine phenotypic and genetic features of strains preliminary classified as Clostridium botulinum species. The phenotypic characteristics were assessed with different culture media and biochemical tests. The genetic characterization included detection of botulinum toxin genes by PCR and macrorestriction analysis with SmaI, XhoI and SacII by PFGE (Pulsed-field Gel Electrophoresis). Despite similar biochemical properties of all analysed strains, only 47% of them contained genes determining toxicity specific to C. botulinum species. The most valuable differentiation of C. botulinum and C. botulinum-like strains was obtained after SmaI digestion. The highest affinity was observed among C. botulinum type B profiles which was even up to 100%. It was found 100% of affinity between C. botulinum and C. botulinum-like strains, however, the similarity among C. botulinum and C. botulinum-like was generally lower than 80%.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Sulphur XANES Analysis of Cultured Human Prostate Cancer Cells
Autorzy:
Kwiatek, W.
Podgórczyk, M.
Paluszkiewicz, Cz.
Balerna, A.
Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/1812260.pdf
Data publikacji:
2008-08
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.kd
87.19.xj
Opis:
Prostate cancer is one of the most commonly diagnosed cancers in men throughout the world. It is believed that changes to the structure of protein binding sites, altering its metabolism, may play an important role in carcinogenesis. Sulphur, often present in binding sites, can influence such changes through its chemical speciation. Hence there is a need for precise investigation of coordination environment of sulphur. X-ray absorption near edge structure spectroscopy offers such possibility. Cell culture samples offer histologically well defined areas of good homogeneity, suitable for successful and reliable X-ray absorption near edge structure analysis. This paper presents sulphur speciation data collected from three different human prostate cancer cell lines (PC-3, LNCaP and DU-145). Sulphur X-ray absorption near edge structure analysis was performed on K-edge structure. The spectra of cells were compared with those of cancerous tissue and with organic substances as well as inorganic compounds.
Źródło:
Acta Physica Polonica A; 2008, 114, 2; 463-470
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Investigation of Trace Element Concentration in Diabetic Rats Tissues
Autorzy:
Kowalska, J.
Krośniak, M.
Gryboś, R.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809705.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
29.30.Ep
78.70.En
87.19.X-
Opis:
Diabetes is one of the most frequent diseases in developing countries and thus there is a significant interest in diabetes related studies. It was found that vanadium compounds have glucose-lowering properties in diabetes and therefore it is very important to estimate the vanadium dose in diabetes treatment. On the other hand, the proper estimation of vanadium concentration is important due to side effects that occur in vanadium supplementation. In this study the influence of V(IV) and V(V) compounds with different ligands on the concentration of K, Ca, Mn, Fe, Cu, and Zn in selected rat's tissues was investigated by means of proton induced X-ray emission technique. As a result of the measurements it was found that the concentration of vanadium depends on the organ. The highest value was determined in spleen while the lowest in pancreas. It was also found that the concentration of other elements depends on the presence of vanadium and its concentration. The most meaningful influence of vanadium presence was on iron concentration in spleen, on copper and zinc in kidney, and on manganese in pancreas.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 556-560
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessing the possibility of genetically modified DNA transfer from GM feed to broiler, laying hen, pig and calf tissues
Autorzy:
Sieradzki, Z.
Mazur, M.
Kwiatek, K.
Swiatkiewicz, S.
Swiatkiewicz, M.
Koreleski, J.
Hanczakowska, E.
Arczewska-Wlosek, A.
Goldsztejn, M.
Powiązania:
https://bibliotekanauki.pl/articles/31445.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to assess the possibility of genetically modified DNA transfer from feed containing RR soybean or/and MON810 maize to animal tissues, gut bacterial flora, food of animal origin, and the fate of GM DNA in the animal digestive tract. The experiment was carried out on broilers, laying hens, pigs and calves. All animals were divided into four groups: I – control group (non-modified feed), II – GM soybean group (non-modified maize, RR soybean), III – GM maize group (MON810 maize, non-modified soybean), and IV – GM maize and soybean group (MON810 maize, RR soybean). Samples of blood, organs, tissues, digesta from the gastrointestinal tract, and eggs were analysed for the presence of plant species specific genes, and transgenic sequences of CaMV 35S promoter and NOS terminator. PCR amplifications of these GM sequences were conducted to investigate the GM DNA transfer from feed to animal tissues and bacterial gut flora. In none of the analysed samples of blood, organs, tissues, eggs, excreta and bacterial DNA were plant reference genes or GM DNA found. A GM crop diet did not affect bacterial gut flora as regards diversity of bacteria species, quantity of particular bacteria species in the animal gut, or incorporation of transgenic DNA to the bacteria genome. It can be concluded that MON810 maize and RR soybean used for animal feeding are substantially equivalent to their conventional counterparts. Genetically modified DNA from MON810 maize and RR soybean is digested in the same way as plant DNA, with no probability of its transfer to animal tissues or gut bacterial flora.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of μ-FTIR-SR Spectroscopy to Prostate Tissue Analysis
Autorzy:
Kwiatek, W.
Paluszkiewicz, C.
Banaś, A.
Kisiel, A.
Podgórczyk, M.
Marcelli, A.
Cestelli Guidi, M.
Piccinini, M.
Powiązania:
https://bibliotekanauki.pl/articles/1810277.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
87.64.km
87.64.kp
87.64.Ee
87.85.jf
Opis:
The infrared spectromicroscopy is a quite recent technique rapidly developing thanks to the availability of new instruments and new brilliant synchrotron radiation sources in different areas and in particular to biomedical researches. In order to achieve a diffraction limited spatial resolution in tissue samples, we performed experiments at SINBAD, the synchrotron infrared beamline of the Laboratori Nazionali di Frascati. We characterized the chemical composition of prostate tissue samples taken from patients affected by prostate cancer disease. Different sizes of the pinholes were considered for the measurements. In the case of prostate tissue sections the results show the possibility to determine the intensity ratio of the $CH_2$ and $CH_3$ bands set at 2930 $cm^{-1}$ and 2960 $cm^{-1}$, respectively. Experiments were also performed with a pinhole of 5 μm of diameter and the differences in both histological and chemical compositions of such samples were determined.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 602-605
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Energy Dissipation in the AFM Elasticity Measurements
Autorzy:
Klymenko, O.
Wiltowska-Zuber, J.
Lekka, M.
Kwiatek, W.
Powiązania:
https://bibliotekanauki.pl/articles/1809585.pdf
Data publikacji:
2009-02
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
68.37.Ps
87.10.Vg
87.15.La
Opis:
Nowadays, it is well established that changes of cell stiffness observed by atomic force microscopy are linked with the cell cytoskeleton. Its structural and functional alterations are underlying major diseases such as cancer, inflammation or neurodegenerative disorders. So far, the use of atomic force microscopy is mostly focused on the determination of the Young modulus using the modified Hertz model. It can quantitatively describe the elastic properties of living cells, however, its value is burdened by the fact that cells are neither isotropic nor homogeneous material. Often, during the atomic force microscopy measurements, the hysteresis between the loading and unloading curves are observed which indicates the dissipation of an energy. In our studies, the index of plasticity was introduced to enumerate such effect during a single loading-unloading cycle. As the results show, such approach delivers an additional parameter describing the mechanical state of cell cytoskeleton. The analysis was performed on test samples where the mechanical properties of the melanoma cells were changed by glutaraldehyde and cytochalasin D treatments. The non-treated cells were compared with fibroblasts.
Źródło:
Acta Physica Polonica A; 2009, 115, 2; 548-551
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 24

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