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Wyszukujesz frazę "microspore culture" wg kryterium: Temat


Wyświetlanie 1-7 z 7
Tytuł:
Anther and microspore cultures of barley and wheat
Autorzy:
Kasha, K J
Ziauddin, A
Cho, U H
Simion, E
Petroski, R
Cistue, L
Powiązania:
https://bibliotekanauki.pl/articles/2046611.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
transformation
isolated microspore
doubled haploid
wheat
microspore culture
barley
anther culture
Opis:
This paper briefly cites the various procedures for the production of doubled haploids in barley and wheat. Various associated terms are defined and the factor involved in haploid production are outlined. Isolated microspore cultures offer some advantages over anther culture. Our current procedures for isolated microspore culture of barley and wheat are presented and compared.
Źródło:
Journal of Applied Genetics; 1997, 38, 4; 373-380
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of cultivation factors on embryogenesis in isolated microspore culture of carrot (Daucus carota L.)
Autorzy:
Voronina, Anastasia V.
Vishnyakova, Anastasiia V.
Monakhos, Sokrat G.
Monakhos, Grigory F.
Ushanov, Alexander A.
Mironov, Aleksey A.
Powiązania:
https://bibliotekanauki.pl/articles/2174344.pdf
Data publikacji:
2022
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
carrot
Daucus carota L.
doubled haploids
embryogenesis
isolated microspore culture
male sterility
Opis:
Using doubled haploid technologies inbreeding can significantly reduce the time to obtain homozygous parental lines required for the production of F1-hybrid of vegetable crops. This study aims to investigate the influence of factors on the efficiency of carrot embryogenesis in isolated microspore culture to optimise the elements of protocol for producing doubled haploids. Microspores were isolated from inflorescences of 21 genotypes and incubated in NLN13 medium supplemented with 0.1 mg·dm-3 2,4-dichlorophenoxyacetic acids, 0.1 mg·dm-3 1-naphthyl acetic acids, 130 g·dm-3 sucrose, and 400 mg·dm-3 casein hydrolysate and its modifications. Embryoids and their groups were formed after 2–6 months, in some cases after 12 months of cultivation. Depending on the variant, the embryogenesis efficiency averaged from 0 to 4.9 embryoids or groups of embryoids per Petri dish (10 cm3). Embryoids within the group were formed from different microspores. No significant effects of inflorescence position on the plant (branching order), sucrose, and casein hydrolysate concentration in the medium were observed. Significant advantages (p ≥ 0.05) for some genotypes were shown: 1) microspore suspension density 4·104 cells·cm-3 (5.0 embryoids per Petri dish were formed at a microspore suspension density of 4·104 cells·cm-3, 0.0 embryoids per Petri dish at a density of 8·104 cells·cm-3); 2) cultivating microspores of tetrad and early mononuclear stage (4.9 ±3.1 embryoids per Petri dish were obtained by culturing tetrads and early mononuclear microspores, while 0.6 ±0.7 embryoids per Petri dish were obtained by culturing of later developmental stages); 3) high-temperature treatment duration of five days (4.9 ±2.1 embryoids per Petri dish were obtained after five days of high-temperature treatment, 2.7 ±2.6 embryoids per Petri dish formed after two days of high-temperature treatment; 9.8 ±4.7, 10.1 ±6.1, 0.0 ±0.0 embryoids per Petri dish formed after two, five and eight days of high-temperature treatment respectively); 4) adding colchicine 0.5 mg·dm-3 to the nutrient medium for two days of high-temperature treatment, followed by medium replacement (3.3 ±2.6 embryoids per Petri dish were obtained by using a nutrient medium with colchicine, while 1.7 ±1.5 embryoids per Petri dish were obtained by culturing in the reference variant).
Źródło:
Journal of Water and Land Development; 2022, 55; 125--128
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tradycyjne i biotechnologiczne metody w hodowli rzepaku jarego [Brassica napus L.] na Litwie
Autorzy:
Sliesaravicius, A
Zilenaite, L.
Burbulis, N.
Duchowski, P.
Powiązania:
https://bibliotekanauki.pl/articles/832810.pdf
Data publikacji:
2003
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
haploidy podwojone
hodowla roslin
kultury izolowanych mikrospor
Brassica napus
Litwa
rzepak ozimy
double haploid
plant breeding
isolated microspore culture
Lithuania
winter rape
Źródło:
Rośliny Oleiste - Oilseed Crops; 2003, 24, 2; 373-382
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of donor plant treatment with 2,4-dichlorophenoxyacetic acid on the anther culture of hot Capsicum spp.
Autorzy:
Nowaczyk, L.
Nowaczyk, P.
Olszewska, D.
Niklas-Nowak, A.
Powiązania:
https://bibliotekanauki.pl/articles/951309.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
androgenesis
Capsicum
capsaicinoid
2,4-dichlorophenoxyacetic acid
anther culture
microspore
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of multi-nucleate microspores in anther culture of salix viminalis L.
Autorzy:
Wojciechowicz, M K
Kikowska, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/41619.pdf
Data publikacji:
2009
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Salix viminalis
genotype
androgenesis
thermal factor
medium composition
stress response
thermal shock
anther culture
multinucleate microspore
male clone
mannitol
Kyo medium
microspore
ultrastructural analysis
initial microspore
Opis:
The aim of the work was to determine how the induction of androgenesis in selected genotypes of Salix viminalis is affected by thermal factors and medium composition. Anthers isolated from male clones of S. viminalis were pre-cultured at 4, 27 and 32°C for two to eight days in liquid Kyo medium with and without the addition of mannitol and on solid MS or WPM medium. The solid media were supplemented with different concentration of disaccharidesand various combinations of growth regulators including kinetin, 2iP, IAA and IBA. Multi-nucleate microspores indicative of androgenesis were observed in anthers pre-cultured for seven days at 4°C in liquid Kyo medium containing mannitol. The rate of androgenesis was higher when the anthers were transferred to solid modified MS medium containing high concentrations of sucrose and kinetin. In studied genotypes of basket willow early uninucleate microspores underwent sporophytic divisions. Ultrastructural observations showed differences in cellular arrangement of pre-stressed microspores. In Salix viminalis, microspores without starch grains and decreased number of lipid bodies were potentially androgenic cells.
Źródło:
Dendrobiology; 2009, 61; 55-64
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale
Autorzy:
Slusarkiewicz-Jarzina, A
Ponitka, A
Powiązania:
https://bibliotekanauki.pl/articles/2046679.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
plant regeneration
microspore
triticale
haploid plant
anther culture
embryonic structure
androgenesis
somatic embryo
Opis:
Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively.
Źródło:
Journal of Applied Genetics; 1997, 38, 3; 253-258
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induced androgenesis in Phleum pratense
Indukowana androgeneza w Phleum pratense
Autorzy:
Broda, Z.
Mikołajczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/2234853.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Łąkarskie
Tematy:
induced androgenesis
androgenesis
Phleum pratense
anther culture
genotype
timothy
medium
anther treatment
homozygous line
in vitro culture
haploid
microspore
plant production
Źródło:
Łąkarstwo w Polsce; 2006, 09; 33-40
1506-5162
Pojawia się w:
Łąkarstwo w Polsce
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-7 z 7

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