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Tytuł:
Porównanie metod oznaczania statycznego kąta zwilżania powierzchni cieczą małocząsteczkową
Comparison between values of sessile drop contact angle depending on method of determination
Autorzy:
Bieliński, D.
Lipiński, P.
Wolska, B.
Jagielski, J.
Powiązania:
https://bibliotekanauki.pl/articles/257952.pdf
Data publikacji:
2006
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Eksploatacji - Państwowy Instytut Badawczy
Tematy:
powierzchnia
kąt zwilżania
aparatura
pomiar
surface
microroughness
polarity
contact angle
instrument
Opis:
Powierzchnię próbek polietylenów: wysokiej gęstości (PE-HD) oraz o bardzo wysokim ciężarze cząsteczkowym (PE-UHMW), poddano bombardowaniu niskoenergetyczną wiązką jonów, uzyskując powierzchnię o zróżnicowanej polarności i mikrochropowatości. Badano kąt zwilżania powierzchni materiałów wodą metodą osadzanej kropli za pomocą różnej aparatury, wykorzystując różne sposoby jego odczytu. Porównano uzyskane wyniki oraz zaprezentowano interpretację powodów rozbieżności w wielkości kąta zwilżania. W analizie wzięto pod uwagę jakość badanej powierzchni - przede wszystkim jej mikrochropowatość, czynniki instrumentalne: objętość kropli i szybkość jej osadzania oraz dokładność, z jaką można wyznaczyć zarys kropli i poziom jej zetknięcia się z badaną powierzchnią.
Surface of polyethylenes: high density (HDPE) and ultrahigh molecular weight (UHMWPE), was subjected to low energy ion beam, producing surfaces of various polarity and microroughness. Sessile drop water contact angle for materials was determined with various instruments, applying different methodology. The results obtained were compared and possible reasons for discrepancies in values of the contact angles, interpreted. The analysis took into consideration quality of the surface studied - mainly its microroughness, instrumentation factors: volume of droplet and speed of its application, as well as precision of a shape of droplet boarder determination and level of its contact with the surface studied.
Źródło:
Problemy Eksploatacji; 2006, 1; 131-144
1232-9312
Pojawia się w:
Problemy Eksploatacji
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Occurrence of intestinal microsporidia in immunodeficient patients in Poland
Autorzy:
Bednarska, M.
Bajer, A.
Sinski, E.
Wolska-Kusnierz, B.
Samolinski, B.
Graczyk, T.M.
Powiązania:
https://bibliotekanauki.pl/articles/51622.pdf
Data publikacji:
2014
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2014, 21, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Identification of Cryptosporidium felis and another opportunistic infections among patients with primary and acquired immunodeficiency
Autorzy:
Bednarska, M.
Bajer, A.
Jankowska, I.
Piwczynska, K.
Wolska-Kusnierz, B.
Wielopolska, M.
Pawelas, A.
Welc-Faleciak, R.
Powiązania:
https://bibliotekanauki.pl/articles/6120.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
identification
Cryptosporidium felis
opportunistic infection
patient
primary immunodeficiency
AIDS
Źródło:
Annals of Parasitology; 2016, 62, Suppl.
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
ZnO Nanopowders by a Microwave Hydrothermal Method - Influence of the Precursor Type on Grain Sizes
Autorzy:
Wolska, E.
Witkowski, B. S.
Godlewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2048108.pdf
Data publikacji:
2011-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.67.-n
61.43.Gt
81.07.Wx
78.55.Et
Opis:
Two series of ZnO nanopowders obtained by a microwave hydrothermal method are examined. We used two different zinc precursors (zinc chloride (ZnCl$\text{}_{2}$) and zinc nitrate hexahydrate (N$\text{}_{2}$O$\text{}_{6}$Zn·6H$\text{}_{2}$O)). Both types of nanopowders show a bright emission in a visible light, including the band edge emission, which indicates their good crystallographic quality. Results of scanning electron microscopy, photo- and cathodoluminescence investigations are presented.
Źródło:
Acta Physica Polonica A; 2011, 119, 5; 683-685
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Successive stages of calcitization and silicification of Cenomanian spicule-bearing turbidites based on microfacies analysis, Polish Outer Carpathians
Autorzy:
Bąk, B.
Górny, Z.
Bąk, K.
Wolska, A.
Stożek, B.
Powiązania:
https://bibliotekanauki.pl/articles/191355.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Geologiczne
Tematy:
calcitization
silicification
sponge spicules
spicule-bearing turbidites
cherts
Cenomanian
Outer Carpathians
Opis:
Mid-Cretaceous turbidites with large proportions of sponge spicules are widely distributed in the Silesian Nappe of the Outer Carpathians, giving rise to diversified types of sediments, from spiculites to spicule-bearing siliciclastics and calcarenites. Part of this succession, Middle–Late Cenomanian in age, was transformed into cherts. A microfacies study showed that these turbidite sediments underwent several stages of calcitization and silicification, which took place during Mid-Cretaceous times in different sedimentary environments, i.e., on a northern shelf bordering the Silesian Basin and on a deep sea floor. The first diagenetic changes were related to changes to the biotic components of the turbidite layers, dominated by siliceous sponge spicules. This process, which took place in the spiculitic carbonate mud on the shelves, was related to the calcitization of sponge spicules. Calcareous clasts and calcified skeletal elements also were corroded by bacteria. After transportation down the slope, the biogenic and siliciclastic particles were deposited below the carbonate compensation depth. Taphonomic processes on the basin floor and alternating phases of carbonate and silica cementations, recrystallization and dissolution occurred in these sediments and were related to the diversification in composition of successive turbidite layers. Silicification was related to the formation of quartz precipitates as fibrous chalcedony or microcrystalline quartz, which were derived from the earlier dissolution of amorphous silica, originating mostly from siliceous sponge spicules and radiolarian skeletons. However, a source of silica from hydrothermal vents was also possible. The initial silica precipitation could have taken place in a slightly acidic environment, where calcite was simultaneously dissolved. A number of silicification stages, visible as different forms of silica precipitate inside moulds after bioclasts, occur in the particular turbidite layers. They were related to changes in various elements of the pore-water profile after descending turbidity-current flows. A very low sedimentation rate during the Middle–Late Cenomanian in the Silesian Basin may have favoured the sequence of initial calcitization and silicification stages of the turbidite sediments.
Źródło:
Annales Societatis Geologorum Poloniae; 2015, 85, 1; 187-203
0208-9068
Pojawia się w:
Annales Societatis Geologorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena składu i stanu ichtiofauny niewielkich cieków środkowej i zachodniej Polski przy zastosowaniu Europejskiego Wskaźnika Ichtiologicznego (EFI+)
Assessment of the composition and state of the fish fauna of small streams in central and west of Poland using the European Fish Index (EFI+)
Autorzy:
Brysiewicz, A.
Czerniejewski, P.
Wolska, M.
Kierasiński, B.
Lipiński, J.
Powiązania:
https://bibliotekanauki.pl/articles/100927.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Stowarzyszenie Infrastruktura i Ekologia Terenów Wiejskich PAN
Tematy:
ryba
mała rzeka
EFI+
monitoring
obszary wiejskie
fish
small river
rural areas
Opis:
Monitoring rzek pod kątem stanu i składu gatunkowego ichtiofauny stanowi jedną z form prowadzenia gospodarki rybackiej wód otwartych. Badania prowadzono na 10-ciu wytypowanych do prac utrzymaniowych ciekach, miały na celu nie tylko określenie składu i zagęszczenia ryb rodzimych i inwazyjnych, ale również poprzez określenie Europejskiego Wskaźnika Ichtiologicznego (EFI+) ocenę stanu ekologicznego wyznaczonych stanowisk w małych ciekach wodnych położonych w dorzeczach Wisły i Odry. W wyniku prowadzonych prac inwentaryzacyjnych odłowiono łącznie 1405 ryb, należących do 24 gatunków. Dominowały gatunki rodzime, jednakże 19% złowionych ryb stanowiły gatunki inwazyjne. Najczęściej spotykanym gatunkiem była płoć (Rutilis rutilus), ale najliczniej notowano kiełbie (Gobio gobio). Na badanych stanowiskach maksymalna wartość Europejskiego Wskaźnika Ocena składu i stanu ichtiofauny niewielkich cieków środkowej i zachodniej polski przy zastosowaniu europejskiego wskaźnika ichtiologicznego (EFI+) Ichtiologicznego wyniosła 0,868. Po podziale na klasy według wartości wskaźnika EFI+ zanotowano, że na większości badanych stanowisk, na których stwierdzono ryby (24 z 28 stanowisk) wartość EFI+ mieściła się w klasie 0,00 - 0,40. Ta stosunkowo mała wartość, świadczyła o słabym stanie siedlisk dla ryb, czego potwierdzeniem były niskie klasy stanu ekologicznego na poszczególnych stanowiskach badawczych. Tylko w przypadku stanowiska 1 na rzece Tywa oraz stanowiska 3 na rzece Czarna-Cedron zanotowano 2 klasę jakości wód. Klasę 3 określono na stanowisku 3 na rzece Tywa. Aż na 15 stanowiskach badawczych stwierdzono klasę 5, która określa zły stan siedlisk ryb i małą ich bioróżnorodność.
River monitoring for the status and species composition of ichthyofauna is one of the forms of open water fisheries management. Research conducted on 10 selected for maintenance works of small rivers was to determine the composition and density of native and invasive fish and estimating the European Fish Index (EFI +) to assess the ecological status of designated sites in these rivers located in the Vistula and Oder basin. A total of 1405 fish, belonging to 24 species, were captured. Native species predominated, however, 19% of the fish caught were invasive species. The most common species was roach (Rutilus rutilus), but the most numerous was the gudgeon (Gobio gobio). At the researched stations, the maximum value of the EFI+ amounted to 0.868. After division into classes according to the value of the EFI + index, the majority of investigated sites where fish were found (24 of 28 sites), the EFI + value was between 0.00 to 0.40. This relatively small value indicated a poor condition of fish habitats, which was confirmed by low classes of ecological status at individual sites. Only in the case of the 1st position in the Tywa river and the 3rd position in the Czarna-Cedron river, there was a water quality class 2. Class 3 was determined at station 3 in the Tywa River. As many as 15 sites have found class 5, which determines the poor condition of fish habitats and low biodiversity.
Źródło:
Infrastruktura i Ekologia Terenów Wiejskich; 2018, II/1; 449-463
1732-5587
Pojawia się w:
Infrastruktura i Ekologia Terenów Wiejskich
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Activity of aspartate aminotransferase and alanine aminotransferase within winter triticale seedlings infested by grain aphid (Sitobion avenae F.)
Autorzy:
Sempruch, C.
Leszczynski, B.
Chrzanowski, G.
Filipczuk, A.
Czerniewicz, P.
Wolska, K.
Powiązania:
https://bibliotekanauki.pl/articles/66072.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Amino acid level is well known indicator of plant resistance to aphids. Our earlier studies showed that grain aphid (Sitobion avenae F.) infestation caused changes in the activity of the enzymes connected with amino acid biosynthesis and the transformation to defensive secondary metabolites within triticale tissues. However, there are not data on the significance of aminotransferases in these processes. The aim of our study was the quantification of changes in the activity of aspartate aminotransferase (AspAT) and alanine aminotransferase (AlaAT) in winter triticale seedlings caused by the feeding of the grain aphid. The study results showed that aphid feeding caused an increase in AlaAT activity and a decrease in AspAT activity within tissues of the triticale. The induced mechanisms of the triticale resistance to the grain aphid are discussed.
Źródło:
Journal of Plant Protection Research; 2012, 52, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
X-ray Absorption Fine Structure Investigation of the Low Temperature Grown ZnCoO Films
Autorzy:
Wolska, A.
Klepka, M.
Witkowski, B.
Witkowski, M.
Guziewicz, E.
Godlewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/1431568.pdf
Data publikacji:
2012-04
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.70.Dm
75.50.Pp
68.55.-a
Opis:
ZnO based diluted magnetic semiconductors are intensively investigated for possible spintronic applications. In the present work we investigate the ZnCoO layers grown at low temperature by atomic layer deposition. The local atomic structure of a series of layers with different Co concentration is investigated by the X-ray absorption fine structure measurements. Two groups of ZnCoO layers are investigated - the ones with an uniform Co distribution and highly nonuniform films. For uniform samples we observe that a majority of Co atoms is built into the ZnO matrix substituting the Zn atoms. In contrast, for the nonuniform samples, metallic Co inclusions are also observed. These results are in strong correlation with the magnetic properties of the films studied separately. Samples with the uniform Co distribution (Co substitutes Zn in ZnO) are paramagnetic, whereas the nonuniform ones show a ferromagnetic response.
Źródło:
Acta Physica Polonica A; 2012, 121, 4; 883-887
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Nowe dane o wieku i petrologii kenozoicznych bazaltoidów dolnośląskich
New data on age and petrological properties of Lower Silesian Cenozoic basaltoids, SW Poland
Autorzy:
Badura, J.
Pécskay, Z.
Koszowska, E.
Wolska, A.
Zuchiewicz, W.
Przybylski, B.
Powiązania:
https://bibliotekanauki.pl/articles/2074475.pdf
Data publikacji:
2006
Wydawca:
Państwowy Instytut Geologiczny – Państwowy Instytut Badawczy
Tematy:
bazaltoidy
petrologia
geochemia
kenozoik
Dolny Śląsk
basaltoids
K-Ar dating
petrology
geochemistry
Cenozoic
Lower Silesia
SW Poland
Opis:
Fifteen samples of basaltoid rocks have been analysed from the Lower Silesia, SW Poland, some of them from localities close to the Sudetic Marginal Fault. K-Ar datings have been made on whole rock samples, using the methodology applied by the Institute of Nuclear Research, Hungarian Academy of Sciences, Debrecen, Hungary. Most of the samples give ages ranging between 21- 38 Ma, whereas that derived from a borehole in the Mokrzeszów Graben has been dated to 44 Ma. Another borehole sample (Jeżów Sudecki B-5), close to the Intrasudetic Fault, is of 59 Ma age. On the other hand, the supposedly "Quaternary" basaltoids from Dębowiec area fall into the interval of 29–30 Ma. The southeasternmost occurrences of the Lower Silesian basaltoids at Nowa Cerekiew display two generations of effusive activity: the older lava flows (26 Ma) are cut by plugs dated to 22 Ma. The Oligocene–Lower Miocene (26-33 Ma, 20'24 Ma) rocks represent alkali basalts and basanites. The alkali basalts consist of phenocrysts of olivine (chrysolite), altered to a different degree, and clinopyroxene (salite). In the matrix, clinopyroxene (Ti-salite), olivine (hyalosiderite), plagioclases (labradorite–andesine), and opaque minerals (titanomagnetite, ilmenite) occur. The basanites contain phenocrysts of altered olivine (chrysolite) and clinopyroxene (salite). Their matrix consists of clinopyroxene (Ti-salites), olivine (hyalosiderite), nepheline, plagioclases (bytownite–andesine), and opaque minerals. On the TAS diagram, samples of the studied basaltoids plot mainly in the fields of basalts and basanites/tephrites. The bimodality of some samples falling into two fields of basalts and basanites/nephelinites on the TiO2/Zr-Nb/y diagram results from their distinct geochemical signature.
Źródło:
Przegląd Geologiczny; 2006, 54, 2; 145-153
0033-2151
Pojawia się w:
Przegląd Geologiczny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation
Autorzy:
Kot, B
Binek, T.
Piechota, M.
Wolska, K.M.
Zdunek, E.
Platkowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/31690.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α- and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference was significant (p≤0.05). The highest percentage of strains from milk of cows with mastitis were weak biofilm formers (48.6%), while 40% and 11.4% of strains were moderate and strong biofilm producers, respectively. S. xylosus showed the highest ability to form biofilm, while the lowest ability to form biofilm was observed in S. aureus and S. epidermidis. In conclusion, production of exotoxins and enzymes, and ability of biofilm formation shown by many CNS isolated from milk of cows with mastitis symptoms indicates that these features are important in pathogenesis of this disease.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photoluminescence and Chromaticity Properties of ZnO Nanopowders Made by a Microwave Hydrothermal Method
Autorzy:
Wolska, E.
Sibera, D.
Witkowski, B.
Yatsunenko, S.
Pełech, I.
Narkiewicz, U.
Godlewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/1492917.pdf
Data publikacji:
2011-11
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
78.67.-n
61.43.Gt
81.07.Wx
78.55.Et
Opis:
Four series of ZnO nanopowders obtained by a microwave hydrothermal method are examined. Two different solvents (ethanol and distilled water) and different values of pressure during heating in the reactor were used. The obtained nanopowders show a bright emission covering visible light spectral region, including the band edge emission. Results of scanning electron microscopy, X-ray diffraction, photo- and cathodoluminescence investigations and also CIE1961 chromaticity diagram are presented.
Źródło:
Acta Physica Polonica A; 2011, 120, 5; 908-910
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Cathodoluminescence Profiling for Checking Uniformity of ZnO and ZnCoO Thin Films
Autorzy:
Witkowski, B. S.
Łukasiewicz, M. I.
Wolska, E. A.
Kopalko, K.
Kowalski, B. J.
Guziewicz, E.
Godlewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2048105.pdf
Data publikacji:
2011-05
Wydawca:
Polska Akademia Nauk. Instytut Fizyki PAN
Tematy:
81.15.Gh
78.30.Fs
78.60.Hk
Opis:
We employ scanning electron microscopy and cathodoluminescence for evaluation of uniformity of ZnCoO films obtained by the atomic layer deposition. Cathodoluminescence quenching by Co ions allows us to detect (regions of weaker light emission) Co accumulations, with the resolution limited by diffusion length of secondary carriers.
Źródło:
Acta Physica Polonica A; 2011, 119, 5; 675-677
0587-4246
1898-794X
Pojawia się w:
Acta Physica Polonica A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Distribution of classical enterotoxin genes in staphylococci from milk of cows with- and without mastitis and the cowshed environment
Autorzy:
Piechota, M.
Kot, B.
Zdunek, E.
Mitrus, J.
Wicha, J.
Wolska, M.K.
Sachanowicz, K.
Powiązania:
https://bibliotekanauki.pl/articles/31921.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to analyze by PCR 185 isolates of Staphylococcus from milk of cows with- and without mastitis and from the cowsheds environment for their potential ability to produce five classical staphylococcal enterotoxins. Among S. aureus isolates 8 (32%) carried enterotoxin genes and only 2 of them had more than one gene. The enterotoxin genes were detected in 22 (13.7%) coagulase-negative staphylococci (CNS) isolates, among them in 9 (11.4%) isolates of S. xylosus, 5 (16.7%) S. sciuri, 3 (10.3%) S. epidermidis and in 5 (22.7%) Staphylococcus spp. In some CNS 2 or 3 genes were detected simultaneously. Among the investigated enterotoxin genes, sec was the most prevalent (70%). The genes encoding enterotoxin B and D were detected in 5 (16.7%) and 6 (20%) isolates, respectively. The lowest number of isolates had sea and see genes.The genes encoding enterotoxins were often identified in staphylococci from milk of cows with mastitis (73.4% of detected genes), while only 6 (20%) isolates from milk of cows without mastitis and 2 (6.6%) isolates from cowshed environment were positive for enterotoxin genes.The results showed that CNS from bovine milk, like S. aureus, carried enterotoxin genes and may pose a risk for public health.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)
Autorzy:
Wolska, K.
Szweda, P.
Lada, K.
Rytel, E.
Gucwa, K.
Kot, B.
Piechota, M.
Powiązania:
https://bibliotekanauki.pl/articles/30220.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twenty two out of 28 strains tested generated unique patterns of DNA bands and only three genotypes consisted of two isolates each were identified. We also tested the P. aeruginosa isolates for their ability to form a biofilm on abiotic surfaces including polyvinylchloride and polystyrene. Different biofilm-forming abilities were demonstrated among strains; however, most of them (64.3%) showed moderate-biofilm forming ability. The strains with increased swimming and twitching motility displayed elevated biofilm formation. However, a negative correlation was found between slime and initial biofilm production. On the basis of the results obtained, we suggest that there are no major differences in phenotypic properties between P. aeruginosa strains isolated from different sources.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 321-329
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk
Autorzy:
Kot, B.
Piechota, M.
Wolska, K.M.
Frankowska, A.
Zdunek, E.
Binek, T.
Klopotowska, E.
Antosiewicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/31274.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this study was to examine phenotypic and genotypic antimicrobial resistance of staphylococci from milk samples from cows with subclinical and clinical mastitis and from cows without mastitis symptoms to methicillin, tetracyclines, macrolides and lincosamides (ML). Of 207 strains, including 34 S. aureus and 173 coagulase-negative staphylococci (CNS), 11 (6.4%) CNS strains were phenotypically resistant to methicillin. The mecA gene was detected by PCR only in two S. xylosus strains and one strain of S. epidermidis and S. simulans. No methicillin-resistant S. aureus strains were observed. In methicillin-resistant strains with mecA, gene resistance to other investigated antibiotics was not observed. Phenotypic resistance to tetracycline was detected in 11.0% of CNS strains and 47.4% of them carried the tetK gene. Of 173 CNS strains studied, 27 (15.6%) were resistant to at least one ML antibiotic. The resistance gene ermC was detected in 55.5% of the 27 ML-resistant strains. The ermA and ermB genes were detected in 14.8% and 11.1% of ML-resistant CNS strains, respectively. Antimicrobial resistance to methicillin, tetracyclines and macrolides was detected more frequently in staphylococcal strains from clinical mastitis compared to animals with subclinical symptoms and without mastitis, while the resistance to lincosamides showed a similar frequency in all groups of cows. In conclusion, CNS species from bovine milk differ in phenotypic and genotypic antimicrobial resistance profiles, and the use of PCR technique alone for the detection of methicillin, macrolide, lincosamide and tetyracycline resistance in CNS from cattle is not reliable.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 17

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