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    Wyświetlanie 1-3 z 3
    Tytuł:
    The effect of triacontanol on shoot multiplication and production of antioxidant compounds in shoot cultures of Salvia officinalis L.
    Autorzy:
    Grzegorczyk, I
    Bilichowski, I.
    Mikiciuk-Olasik, E.
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/56937.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    triacontanol
    shoot multiplication
    production
    antioxidant compound
    shoot culture
    Salvia officinalis
    carnosic acid
    carnosol
    rosmarinic acid
    Opis:
    This report describes the effect of triacontanol on shoot multiplication and production of antioxidant compounds (carnosic acid, carnosol and rosmarinic acid) in S. officinalis cultures grown on MS basal medium (agar solidified medium supplemented with 0.1 mg l-1 IAA, 0.45 mg l-1 BAP). It was found that shoot proliferation significantly increased when triacontanol at concentrations of 5, 10 or 20 µg l-1 was added to the medium. HPLC analysis of acetone and methanolic extracts of sage shoots showed that the production of diterpenoids, carnosic acid/carnosol ratio, as well as, contents of rosmarinic acid were also affected by the treatment with triacontanol. The highest stimulation effect of triacontanol was observed on the production of carnosol, where the treatment with 20 µg l l-1 increased the content of this diterpenoid 4.5-fold compared to that in the control (sage shoots growing on MS basal medium, only).
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2006, 75, 1; 11-15
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Liquid shoot culture of Salvia officinalis L. for micropropagation and production of antioxidant compounds; Effect of triacontanol
    Autorzy:
    Grzegorczyk, I
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/58539.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    Salvia officinalis
    medicinal plant
    triacontanol
    liquid shoot culture
    botany
    herb
    micropropagation
    production
    antioxidant compound
    sage
    Opis:
    Liquid shoot culture of Salvia officinalis L. in MS medium containing IAA (0.1 mg l-1) and BAP (0.45 mg l-1) was developed and evaluated in relation to shoot multiplication and antioxidant compound (carnosic acid, carnosol and rosmarinic acid) accumulation. In the liquid medium, on average, 3 new shoots per explant (shoot tip) were obtained within 3 weeks. The shoots produced 8.2±0.02 mg of diterpenoids and 31.2±0.29 mg of rosmarinic acid per gram of dry weight. Shoot proliferation and diterpenoid content increased when triacontanol (5, 10 or 20 pg l-1) was added to the liquid medium. In optimum conditions (at 20 pg l-1 TRIA) almost 7 shoots were formed per explant after 3 weeks. An increase in diterpenoid production (expressed as the sum of carnosol and carnosic acid) ranged from 30% to 50% and dependended on triacontanol concentration tested. The level of diterpenoids in triacontanol-treated shoots was similar to the content of compounds in commercial herbal product (dried leaves of S. officinalis) (10-12 mg g-1 dry wt). Triacontanol did not increase rosmarinic acid production, but the content of the phenolic as compound in shoots grown in liquid culture (31 mg g-1 dry wt) was even 24 times higher compared to samples of dried leaves of S. officinalis plants. We also demonstrated that the highest amounts of CA, Car and RA were accumulated in young, top parts of sage shoots. This observation could be useful for improving the selection of material for the extraction of natural antioxidants from S. officinalis.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2008, 77, 2
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Antioxidant compounds in Salvia officinalis L. shoot and hairy root cultures in the nutrient sprinkle bioreactor
    Autorzy:
    Grzegorczyk, I
    Wysokinska, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/59229.pdf
    Data publikacji:
    2010
    Wydawca:
    Polskie Towarzystwo Botaniczne
    Tematy:
    antioxidant compound
    Salvia officinalis
    shoot
    hairy root
    root culture
    nutrient sprinkle bioreactor
    bioreactor
    leaf
    stem
    Opis:
    The study focused on the production of compounds with antioxidant activity in hairy root and shoot cultures of Salvia officinalis grown in laboratory-scale sprinkle nutrient bioreactors. HPLC analysis showed that production of rosmarinic acid in transformed roots (34.65±1.07 mg l-1) was higher that in shoot culture (26.24±0.48 mg l-1). In the latter diterpenoids: carnosic acid (1.74±0.02 mg l-1) and carnosol (1.34±0.01 mg l-1) were also found. Biomass accumulation after a growth period in the bioreactor was also studied. An 18-fold increase in hairy root biomass was recorded after 40 days of culture. In sage shoot culture, biomass increased 43 times after 21 days of bioreactor run. The current operating conditions of the bioreactor were not suitable for the propagation of Salvia officinalis mainly due to the hyperhydricity problem of leaves and stems.
    Źródło:
    Acta Societatis Botanicorum Poloniae; 2010, 79, 1; 7-10
    0001-6977
    2083-9480
    Pojawia się w:
    Acta Societatis Botanicorum Poloniae
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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