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Wyszukujesz frazę "Wójcik, A.J." wg kryterium: Autor


Tytuł:
The occurrence of Toxoplasma gondii and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from East Poland with the use of PCR
Autorzy:
Sroka, J
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/51137.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Polska
tick
Ixodes ricinus
Toxoplasma gondii
Borrelia burgdorferi
occurrence
polymerase chain reaction
genotyping
B1 gene
human population
infection
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 313-319
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pairs of primers detecting 16S rRNA and ankA genes
Autorzy:
Chmielewska-Badora, J
Zwolinski, J.
Cisak, E.
Wojcik-Fatla, A.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51023.pdf
Data publikacji:
2007
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lublin region
Polska
pathogen
Ixodes ricinus
Eastern Poland
tick
determination
animal pathogen
human pathogen
ankA gene
16S rRNA gene
granulocytic anaplasmosis
bacteria
Anaplasma phagocytophilum
polymerase chain reaction
Opis:
A total of 684 Ixodes ricinus ticks (321 nymphs, 184 males, and 179 females) were collected by fl agging lower vegetation in 6 forest districts located on the territory of Lublin province (eastern Poland). Ticks were examined by polymerase chain reaction (PCR) method for the presence of Anaplasma phagocytophilum DNA with two pairs of primers: EHR521/EHR747 for detecting 16S rRNA gene, and LA6/LA1 for detecting ankA gene. To study the relationship between infection in ticks and people occupationally exposed to tick bite, blood serum samples of 261 forestry workers employed in the same forest districts were examined by immunofl uorescence method for the presence of specifi c antibodies against A. phagocytophilum. A total of 70 ticks out of 684 examined (10.2%) showed the presence of A. phagocytophilum 16S rRNA gene. The prevalence of infection was signifi cantly dependent on tick’s stage (χ2=49.2, p<0.00001) and geographical locality (χ2=34.4, p<0.00001). The percentage of I. ricinus females infected with A. phagocytophilum (24.6%) was signifi cantly greater compared to males (6.5%) and nymphs (4.4%) (p<0.00001). Only 19 ticks out of 684 examined (2.8%) showed the presence of A. phagocytophilum ankA gene, signifi cantly less compared to 16S rRNA gene (p<0.00001). The prevalence of infection demonstrated by the presence of ankA gene was also signifi cantly dependent on tick’s stage (χ2=23.6, p<0.00001) but not on locality (χ2=9.8, p=0.082). A signifi cant correlation was found between the presence of A. phagocytophilum 16S rRNA gene in I. ricinus female ticks from the particular forest districts and the serologic response to A. phagocytophilum of forestry workers employed in the same districts (p<0.05). No signifi cant correlation was found between the presence of A. phagocytophilum ankA gene in I. ricinus ticks and serologic response of exposed workers. In conclusion, detection of A. phagocytophilum infection in ticks by PCR with the use of EHR521/EHR747 primers detecting 16S rRNA gene is signifi cantly more sensitive compared to LA6/LA1 primers detecting ankA gene.
Źródło:
Annals of Agricultural and Environmental Medicine; 2007, 14, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA was not detected in Dermacentor reticulatus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51730.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Opis:
A total of 190 Dermacentor reticulatus ticks (80 males, 110 females) collected on the territory of Ostrów Lubelski, Suchawa, Zalutyń and Kazimierz Dolny (Lublin Province, eastern Poland) were examined by reverse transcription PCR and nested PCR methods for the presence of hantavirus RNA. None of the examined Dermacentor reticulatus specimens showed the presence of the hantavirus-specific RNA in spite of using two pairs of primers and the clearly positive results obtained with the positive control. Thus, the hypothesis about the possible participation of ticks in the transmission of hantaviruses was not confirmed.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. II. Examination of native samples from various sources
Autorzy:
Stojek, N.M.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50046.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
A total of 123 water samples were examined in parallel by culture and semi-nested PCR for the presence of Legionella. They comprised: 35 samples of hot water distributed by the urban municipal water supply system (MWSS) taken in institutions, 45 samples of hot water distributed by urban MWSS taken in dwellings, 27 samples of cold water distributed by rural MWSS taken in dwellings, and 16 samples of cold well water taken in rural areas. The greatest frequency of the isolation of Legionella by culture (88.6%) was recorded in the samples of hot water from the urban institutions, having been greater compared to all other sources (p<0.001). The frequency of Legionella isolation from hot water in urban dwellings (28.9%) was significantly greater compared to the combined value (2.3%) for cold water from rural MWSS and wells (p<0.001). Strains belonging to Legionella pneumophila serogroups 2-14 predominated in the examined samples, while strains of L. pneumophila serogroup 1 and strains of Legionella spp. (other than L. pneumophila) were 3-fold less numerous. The rates of positive findings in the semi-nested PCR (stage 2) were greater than culture isolations in all kinds of samples, except for urban institutions. The correlation between the culture and PCR results was positive for samples of hot water from urban MWSS (p<0.01), but not for samples of cold water from rural MWSS and wells (p>0.5). A significant correlation was found between rates of PCRpositive results and numbers of Legionella pneumophila serogroups 2-14 strains, but not for other Legionella serogroups or species. In conclusion, our results support the opinion that though PCR cannot be a substitute for the isolation of Legionella by culture, it could be regarded as an useful complementary method.
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efficacy of the detection of Legionella in hot and cold water samples by culture and PCR. I. Standardization of methods
Autorzy:
Wojcik-Fatla, A.
Stojek, N.M.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50884.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Opis:
The aim of the present study was: – to compare methods for concentration and isolation of Legionella DNA from water; – to examine the efficacy of various modifications of PCR test (PCR, semi-nested PCR, and real-time PCR) for the detection of known numbers of Legionella pneumophila in water samples artificially contaminated with the strain of this bacterium and in randomly selected samples of environmental water, in parallel with examination by culture. It was found that filtration is much more effective than centrifugation for the concentration of DNA in water samples, and that the Qiamp DNA Mini-Kit is the most efficient for isolation of Legionella DNA from water. The semi-nested PCR and real-time PCR proved to be the most sensitive methods for detection of Legionella DNA in water samples. Both PCR modifications showed a high correlation with recovery of Legionella by culture (p<0.01), while no correlation occurred between the results of one-stage PCR and culture (p>0.1).
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The occurrence of Toxoplasma gondii infection in people and animals from rural environment of Lublin region - estimate of potential role of water as a source of infection
Autorzy:
Sroka, J
Wojcik-Fatla, A.
Szymanska, J.
Dutkiewicz, J.
Zajac, V.
Zwolinski, J.
Powiązania:
https://bibliotekanauki.pl/articles/49789.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
parasite
protozoan parasite
Toxoplasma gondii
occurrence
people
animal
domestic animal
rural environment
Lublin region
epidemiology
serological examination
farm inhabitant
potable water
polymerase chain reaction
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 1; 125-132
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of tick-borne encephalitis virus [TBEV] in samples of raw milk taken randomly from cows, goats and sheep in Eastern Poland
Autorzy:
Cisak, E
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Buczek, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50068.pdf
Data publikacji:
2010
Wydawca:
Instytut Medycyny Wsi
Tematy:
tick-borne encephalitis virus
prevalence
raw milk
milk
milk sample
cow
goat
sheep
Polska
epidemiology
antibody
Źródło:
Annals of Agricultural and Environmental Medicine; 2010, 17, 2; 283-286
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Study on tick-borne rickettsiae in eastern Poland. I. Prevalence in Dermacentor reticulatus (Acari: Amblyommidae)
Autorzy:
Wojcik-Fatla, A.
Cisak, E.
Zajac, V.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/50634.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
Rickettsia
Rickettsia raoultii
Polska
prevalence
Dermacentor reticulatus
tick
Acari
Amblyommidae
Opis:
Rickettsia spp. transmitted by ticks are classified mostly in the Spotted Fever Group Rickettsiae (SFGR). Numerous species of this group have been identified in Eurasia as emerging pathogens, but still little is known about their occurrence, effects on human health, and co-incidence with other tick-borne pathogens. The aim of the presented study was to determine the prevalence of Rickettsia spp. in adult Dermacentor reticulatus (Acari: Amblyommidae) ticks collected in Lublin province of eastern Poland using the PCR method. The infection rate of D. reticulatus with Rickettsia spp. was 53.0%. All except one rickettsial isolates showed 100% homology with Rickettsia raoultii. A high prevalence of R. raoultii in Dermacentor reticulatus ticks from eastern Poland suggests that the SFGR species should be considered as potential causative agents of tick-borne diseases in this area.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Cisak, E.
Sroka, J.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51864.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 4
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroepidemiological study of Lyme borreliosis among forestry workers in Southern Poland
Autorzy:
Buczek, A
Rudek, A.
Bartosik, K.
Szymanska, J.
Wojcik-Fatla, A.
Powiązania:
https://bibliotekanauki.pl/articles/49580.pdf
Data publikacji:
2009
Wydawca:
Instytut Medycyny Wsi
Tematy:
Lyme borreliosis
borreliosis
serology
ELISA test
forestry
worker
Polska
forestry worker
tick-borne disease
diagnosis
antibody
treatment
Borrelia burgdorferi
tick
immunoglobulin
Źródło:
Annals of Agricultural and Environmental Medicine; 2009, 16, 2; 257-261
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland
Autorzy:
Cisak, E.
Wojcik-Fatla, A.
Zajac, V.
Sroka, J.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/52094.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ocena wspolwystepowania Toxaplasma gondii i Borrelia burgdorferi w kleszczach Ixodes ricinus w oparciu o badania PCR
Autorzy:
Wojcik-Fatla, A.
Sroka, J.
Zajac, V.
Cisiak, E.
Powiązania:
https://bibliotekanauki.pl/articles/839306.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty
Toxoplasma gondii
Borrelia burgdorferi
wspolwystepowanie
kleszcze
Ixodes ricinus
metody badan
lancuchowa reakcja polimerazy
Źródło:
Annals of Parasitology; 2010, 56, 3; 207
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Risk of tick-borne diseases in various categories of employment among forestry workers in eastern Poland
Autorzy:
Cisak, E.
Zajac, V.
Wojcik-Fatla, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51019.pdf
Data publikacji:
2012
Wydawca:
Instytut Medycyny Wsi
Źródło:
Annals of Agricultural and Environmental Medicine; 2012, 19, 3
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A small-scale survey of hantavirus in mammals from eastern Poland
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Sroka, J.
Cisak, E.
Sawczyn, A.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/51577.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
Bunyaviridae
mammal
small mammal
Microtus agrestis
Myodes glareolus
Sorex araneus
epidemiology
polymerase chain reaction
flood
Polska
Opis:
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł

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