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Wyświetlanie 1-3 z 3
Tytuł:
Heme synthesis in yeast does not require oxygen as an obligatory electron acceptor.
Autorzy:
Krawiec, Zdzisława
Święciło, Agata
Biliński, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/1044224.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
osmotic stress
catalase T
catalase A
heat shock
Saccharomyces cerevisiae anoxia
Opis:
In a previous paper (Krawiec, Z., Biliński, T., Schüller, C. & Ruis, H., 2000, Acta Biochim. Polon. 47, 201-207) we have shown that catalase T holoenzyme is synthesized in the absence of oxygen after treatment of anaerobic yeast cultures with 0.3 M. NaCl, or during heat shock. This finding suggests that heme moiety of the enzyme can either be formed de novo in the absence of oxygen, or derives from the preexisting heme pool present in cells used as inoculum. The strain bearing hem1 mutation, resulting in inability to form δ-aminolevulinate (ALA), the first committed precursor of heme, was used in order to form heme-depleted cells used as inocula. The cultures were supplemented with ALA at the end of anaerobic growth prior the stress treatment. The appearance of active catalase T in the stressed cells strongly suggests that heme moiety of catalase T is formed in the absence of oxygen. This finding suggests the necessity to reconsider current opinions concerning mechanisms of heme synthesis and the role of heme as an oxygen sensor.
Źródło:
Acta Biochimica Polonica; 2000, 47, 4; 1027-2035
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Reactive oxygen species as second messengers? Induction of the expression of yeast catalase T gene by heat and hyperosmotic stress does not require oxygen.
Autorzy:
Krawiec, Zdzisława
Biliński, Tomasz
Schüller, Christoph
Ruis, Helmut
Powiązania:
https://bibliotekanauki.pl/articles/1044432.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
yeast.
osmotic stress
thermotolerance
catalase T
oxygen
Saccharomyces cerevisiae
stress response
heat shock
Opis:
It is shown that oxygen is not absolutely needed for stress-induced synthesis of catalase T in the yeast Saccharomyces cerevisiae. Yeast cells develop heat resistance after exposure to elevated temperatures in anoxia. The levels of catalase activity and thermotolerance are comparable to those in aerobically stressed cells. While these results obviously do not exclude a stress signaling role of reactive oxygen species in some systems, as postulated by other authors, they suggest that the question of the obligatory requirement for reactive oxygen species in other stress signaling systems should be rigorously re-investigated.
Źródło:
Acta Biochimica Polonica; 2000, 47, 1; 201-207
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of stress on the life span of the yeast Saccharomyces cerevisiae.
Autorzy:
Święciło, Agata
Krawiec, Zdzisława
Wawryn, Jarosław
Bartosz, Grzegorz
Biliński, Tomasz
Powiązania:
https://bibliotekanauki.pl/articles/1044359.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
aging
osmotic stress
superoxide dismutase
catalase
Saccharomyces cerevisiae
heat shock
oxidative stress
Opis:
A correlation is known to exist in yeast and other organisms between the cellular resistance to stress and the life span. The aim of this study was to examine whether stress treatment does affect the generative life span of yeast cells. Both heat shock (38°C, 30 min) and osmotic stress (0.3 M NaCl, 1 h) applied cyclically were found to increase the mean and maximum life span of Saccharomyces cerevisiae. Both effects were more pronounced in superoxide dismutase-deficient yeast strains (up to 50% prolongation of mean life span and up to 30% prolongation of maximum life span) than in their wild-type counterparts. These data point to the importance of the antioxidant barrier in the stress-induced prolongation of yeast life span.
Źródło:
Acta Biochimica Polonica; 2000, 47, 2; 355-364
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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