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Wyświetlanie 1-3 z 3
Tytuł:
In vitro antimicrobial activity of extracts and their fractions from three Eryngium L. species
Właściwości przeciwbakteryjne ekstraktów i ich frakcji trzech gatunków Eryngium L.
Autorzy:
Kikowska, M.
Dlugaszewska, J.
Kubicka, M.M.
Kedziora, I.
Budzianowski, J.
Thiem, B.
Powiązania:
https://bibliotekanauki.pl/articles/72690.pdf
Data publikacji:
2016
Wydawca:
Instytut Włókien Naturalnych i Roślin Zielarskich
Opis:
Introduction: Due to increasing resistance against antibiotics and antifungal agents, crude plant extracts, fractions, and isolated pure compounds became a new interest as antimicrobial agents. Objectives: The antimicrobial activity of methanolic extracts and fractions of Eryngium planum L., E. campestre L., and E. maritimum L. was evaluated against selected bacteria, yeast and mould, and compared in tested Eryngium species and in their organs. Methods: The antimicrobial activity was studied with use of broth microdilution method. The antibacterial (Staphylococcus aureus, Pseudomonas aeruginosa) and antifungal (Candida albicans, Aspergillus niger) activity of selected extracts and fractions compared with the reference substance was expressed by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal/Fungicidal Concentration (MBC/MFC).The extract and fraction compounds were identified on the basis of TLC examination. Results: The saponin-phenolic acid fractions of E. maritimum and E. planum and a saponin fraction of E. planum showed the highest activity against S. aureus (MIC = 1–2.5 mg·ml-1). The growth of C. albicans was inhibited by methanolic extract of E. planum cell suspension culture (MIC = 7.8 mg·ml-1). Conclusion: The antimicrobial activity depends on the Eryngium species, tested biomass, and microorganism.
Wstęp: Z powodu rosnącej oporności na antybiotyki i środki przeciwgrzybicze rośnie zainteresowanie ekstraktami roślinnymi, frakcjami i wyizolowanymi czystymi związkami jako środkami przeciwdrobnoustrojowymi. Cel: Badano aktywność przeciwdrobnoustrojową ekstraktów metanolowych i frakcji Eryngium planum L., E. campestre L. i E. maritimum L. w stosunku do wybranych bakterii, drożdżaka i grzyba pleśniowego oraz porównywano badane gatunki Eryngium i ich organy. Metody: Aktywność przeciwdrobnoustrojową badano z zastosowaniem metody seryjnych rozcieńczeń w bulionie. Aktywność przeciwbakteryjna (Staphylococcus aureus, Pseudomonas aeruginosa) i przeciwgrzybicza (Candida albicans, Aspergillus niger) wybranych ekstraktów i frakcji, w porównaniu z substancją referencyjną, wyrażona została za pomocą minimalnego stężenia hamującego (MIC) i minimalnego stężenia bakterio- lub grzybobójczego (MBC/MFC). Wyniki: Frakcje saponinowo-fenolokwasowe E. maritimum i E. planum oraz frakcja saponinowa E. planum wykazały najwyższą aktywność wobec S. aureus (MIC = 1–2,5 mg∙ml -1). Wzrost C. albicans był hamowany przez metanolowy ekstrakt zawiesiny komórkowej E. planum (MIC = 7,8 mg∙ml -1). Wnioski: Aktywność przeciwdrobnoustrojowa zależy od gatunku Eryngium, testowanej biomasy i mikroorganizmu.
Źródło:
Herba Polonica; 2016, 62, 2
0018-0599
Pojawia się w:
Herba Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Micropropagation of Eryngium campestre L. via shoot culture provides valuable uniform plant material with enhanced content of phenolic acids and antimicrobial activity
Autorzy:
Kikowska, M.
Thiem, B.
Sliwinska, E.
Rewers, M.
Kowalczyk, M.
Stochmal, A.
Dlugaszewska, J.
Powiązania:
https://bibliotekanauki.pl/articles/19447.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro- and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg lˉ¹ benzyladenine (BA) and 0.1 mg lˉ¹ indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2016, 58, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of antiamoebic and antimicrobial activities in vitro of Chaenomeles japonica (Thunb.) Lindl. ex Spach extracts
Autorzy:
Klikowska, M.
Derda, M.
Thiem, B.
Wlodarczyk, A.
Dlugaszewska, J.
Stochmal, A.
Zuchowski, J.
Hadas, E.
Powiązania:
https://bibliotekanauki.pl/articles/2117842.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Acanthamoeba spp.
chaenomeles japonica
pentacyclic terpenoids
plant extracts
plant
in vitro cultures
polyphenols
staphylococcus aureus
Opis:
The free-living Acanthamoeba sp. causes various diseases. Treatment of them is very difficult and not always effective because of encystation, making it highly resistant to antiamoebic drugs. Gram-positive bacteria staphylococcus aureus, Gram-negative bacteria Escherichia coli, and an yeast candida albicans also exhibit outstanding resistance to antimicrobial substances. The search for new natural amoebicidal and antimicrobial agents of plant origin is still of current interest. The aim of the study was to investigate the amoebicidal activity of the extracts obtained from tissue culture and a field-grown plant of chaenomeles japonica against pathogenic trophozoites of Acanthamoeba spp. and antimicrobial effect against s. aureus, E. coli, and c. albicans. The extracts of c. japonica had an inhibitory effect on the proliferation of Acanthamoeba trophozoites as compared to the non-treated control. among the crude extracts tested, the extract of leaves, from both shoot culture and the field-grown plant had remarkable amoebicidal action against the trophozoites but also antibacterial activity against Gram-positive bacteria staphylococcus aureus. The extract from leaves from shoot culture, already on the second and third days of treatment, showed an antiamoebicidal effect at a concentration of 1 mg ml-1 (inhibi- tion of trophozoites 87.5% and 91.8%, respectively). In addition to leaves from shoot culture (a conc. 5 mg ml-1, 2nd day inhibition of trophozoites 85.7% and 3rd day 97.2%), leaves from a field-grown plant (a conc. 5 mg ml-1, 2nd day 91.0% and 3rd day 94.4%) and callus (a conc. 5 mg ml-1, 2nd day 90.0% and 3rd day – 95.4%) also exhib- ited a good antiamoebicidal activity. Out of the four extracts, the extracts from leaves from both shoot culture and a field-grown plant were reported to be the most active against Gram-positive s. aureus, which was determined by the values of MIC = 5.0 mg ml-1 and MIC = 2.5 mg ml-1, respectively. The inhibitory potential depends on the yield and composition of mainly bioactive compounds: pentacyclic terpenoids (mainly betulinic, ursolic, and oleanolic acids) and polyphenols (mainly chlorogenic acid and its isomers, epicatechin, dimeric, and trimeric proanthocyanidins, quercetin and kaempferol derivatives).
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 2; 47-58
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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