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Wyświetlanie 1-15 z 35
Tytuł:
Conifer somatic embryogenesis: I. Development
Autorzy:
Klimaszewska, K
Cyr, D.R.
Powiązania:
https://bibliotekanauki.pl/articles/41304.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
somatic seedling
initiation
development
somatic embryogenesis
Pinaceae
maturation
embryogenesis
conifer species
forest industry
forest
somatic tree
cryopreservation
Opis:
The discovery of conifer somatic embryogenesis (SE) and the subsequent development of SE protocols for a range of genera and species have opened new research opportunities to forest biotechnologists and a means towards mass clonal propagation for the forest industry. This paper provides a general description of the conifer SE process, followed by a review of protocols developed specifically for several conifer species in the Pinaceae family for which production of somatic trees has been demonstrated. Additionally, future research needs, including approaches for developing markers to optimize SE process and the production of high quality embryos, are discussed.
Źródło:
Dendrobiology; 2002, 48
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Conifer somatic embryogenesis: II. Applications
Autorzy:
Cyr, D R
Klimaszewska, K.
Powiązania:
https://bibliotekanauki.pl/articles/41451.pdf
Data publikacji:
2002
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
plantation forestry
somatic seedling
application
somatic embryogenesis
Pinaceae
embryogenesis
germplasm preservation
clonal propagation
Opis:
Somatic embryogenesis (SE) of conifers for clonal propagation has emerged from its earliest beginnings in 1980 to become an integral component of tree improvement strategies. With its capacity for long-term germplasm preservation and scale-up technologies,it is seen to be the preferred avenue to accelerate selection and operational deployment of value-added genotypes. At present,programs for numerous species from the Pinaceae family are underway worldwide,with activities ranging from selection trials to pilot production for plantation forestry. This paper will provide a review of current efforts in conifer SE including cryopreservation,commercialization and deployment strategies,and transgenics. A discussion of challenges and issues is directed at genetic fidelity, intellectual property and future needs.
Źródło:
Dendrobiology; 2002, 48
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of PEG 4000 on maturation, germination and conversion frequency of Picea abies and P. omorika somatic embryos
Autorzy:
Wawrzyniak, M.
Hazubska-Przybyl, T.
Obarska, A.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/951243.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
polyethylene glycol
somatic embryogenesis
somatic seedling
maturation
germination
coniferous species
Picea abies
Picea omorika
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Role of the polycomb repressive complex (PRC2) in the induction of somatic embryogenesis Medicago truncatula cv. Jemalong
Autorzy:
Orlowska, A.
Igielski, R.
Kepczynska, E.
Powiązania:
https://bibliotekanauki.pl/articles/951214.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
somatic embryogenesis
somatic cell
marker gene
leafy cotyledon1
embryogenesis
polycomb repressive complex
Medicago truncatula
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Tree somatic embryogenesis in science and forestry
Autorzy:
Hazubska-Przybyl, T.
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/41255.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
tree
somatic embryogenesis
embryogenesis
science
forestry
in vitro culture
seedling
somatic seedling
forest management
Opis:
Somatic embryogenesis is the latest, and potentially the most efficient, method for the vegetative micropropagation of plants. Over the past three decades, numerous laboratory studies have investigated somatic embryogenesis of forest trees, yielding positive results for a number of economically important tree species. The first test trials were run and plantations were planted with interior spruce in the 90s by CellFor Inc. (Canada). However, at the beginning of the XXI century, the program to produce spruce and Douglas fir somatic seedlings was stopped for economic reasons. Thus, currently no operational program is ongoing except on a small scale in New Brunswick. In order to integrate somatic embryogenesis technology into operational reforestation programs, the production costs of forest tree somatic seedlings needs to be reduced, and the awareness of foresters and forest landowners that the material obtained through somatic embryogenesis is valuable needs to be increased. This awareness would enable implementation of this technology on a large scale for production and forest management throughout Europe including Poland. In this review, the importance of somatic embryogenesis in scientific research and in global and European forestry is presented. Our main aims are to provide basic information on the challenges in researching somatic embryogenesis of forest trees and to raise interest in this tree propagation technique in both scientists and foresters.
Źródło:
Dendrobiology; 2016, 76
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somaclonal variation during Picea abies and P. omorika somatic embryogenesis and cryopreservation
Autorzy:
Hazubska-Przybył, Teresa
Dering, Monika
Powiązania:
https://bibliotekanauki.pl/articles/952041.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
microsatellites
somaclonal variations
somatic embryogenesis
spruce
Opis:
Embryogenic cultures of plants are exposed to various stress factors both in vitro and during cryostorage. In order to safely include the plant material obtained by somatic embryogenesis in combination with cryopreservation for breeding programs, it is necessary to monitor its genetic stability. The aim of the present study was the assessment of somaclonal variation in plant material obtained from embryogenic cultures of Picea abies (L.) Karst. and P. omorika (Pančić) Purk. maintained in vitro or stored in liquid nitrogen by the pregrowth-dehydration method. The analysis of genetic conformity with using microsatellite markers was performed on cotyledonary somatic embryos (CSE), germinating somatic embryos (GSE) and somatic seedlings (SS), obtained from tissues maintained in vitro or from recovered embryogenic tissues (ETc) and CSE obtained after cryopreservation. The analysis revealed changes in the DNA of somatic embryogenesis-derived plant material of both Picea spp. They were found in plant material from 8 out of 10 tested embryogenic lines of P. abies and in 10 out of 19 embryogenic lines of P. omorika after in vitro culture. Changes were also detected in plant material obtained after cryopreservation. Somaclonal variation was observed in ETc and CSE of P. omorika and at ETv stage of P. abies. However, most of the changes were induced at the stage of somatic embryogenesis initiation. These results confirm the need for monitoring the genetic stability of plants obtained by somatic embryogenesis and after cryopreservation for both spruce species.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
High activity of auxin response factors (ARF5, ARF6, ARF10 and ARF16) in somatic embryogenesis of Arabidopsis
Autorzy:
Wojcikowska, B.
Gaj, M.D.
Powiązania:
https://bibliotekanauki.pl/articles/80554.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
high activity
auxin response factor
indole-3-acetic acid
somatic embryogenesis
Arabidopsis
somatic cell
zygotic embryogenesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regeneration of somatic embryos from in vitro isolated ligulate florets of chrysanthemum
Regeneracja zarodków somatycznych z izolowanych in vitro kwiatów języczkowatych chryzantemy
Autorzy:
Tymoszuk, A.
Zalewska, M.
Lema-Rumińska, J.
Powiązania:
https://bibliotekanauki.pl/articles/11542967.pdf
Data publikacji:
2014
Wydawca:
Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
Tematy:
embryo regeneration
somatic embryo
in vitro culture
isolation
ligulate floret
chrysanthemum
Dendranthema grandiflora
somatic embryogenesis
growth regulator
Opis:
Many chrysanthemum mutants are chimeras built from tissues of a varied genetic composition. Regeneration in vitro of somatic embryos from the whole mutated ligulate florets or only from their small mutated fragments can lead to the separation of chimera components and, as a result, producing a new original cultivar. There was determined the effect of growth regulators (2,4-D; KIN or BAP) and explant type (whole or transversely-cut-into-half ligulate florets) on the efficiency of somatic embryogenesis of Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’. For the induction of the somatic embryogenesis ant the regeneration of somatic embryos the MS medium [Murashige and Skoog 1962] with 18.08 μM 2,4-D as well as with this auxin and 4.44; 8.88; 22.20 μM BAP or 4.65; 9.30; 23.25 μM KIN was used. The best results were obtained when transversely-cut-into-half ligulate florets were inoculated onto the medium with 4.65 μM KIN and 18.08 μM 2,4-D. This results may increase the probability of success in separation of chimera components in chrysanthemum breeding.
Wiele mutantów chryzantem jest chimerami zbudowanymi z tkanek o różnym składzie genetycznym. Regeneracja in vitro zarodków somatycznych z całych objętych mutacją kwiatów języczkowatych lub tylko z ich niewielkich zmutowanych fragmentów może doprowadzić do separacji komponentów składowych chimery i w rezultacie do uzyskania nowej, oryginalnej odmiany. W badaniach określono wpływ regulatorów wzrostu (2,4-D; KIN lub BAP) oraz rodzaju eksplantatu (cały lub przecięty poprzecznie na pół kwiat języczkowaty) na wydajność embriogenezy somatycznej u Chrysanthemum × grandiflorum (Ramat.) Kitam. ‘Cool Time’. Do indukcji embriogenezy somatycznej oraz do regeneracji zarodków somatycznych zastosowano pożywkę MS [Murashige and Skoog 1962] uzupełnioną jedynie 18,08 μM 2,4-D, a także tą auksyną oraz 4,44; 8,88; 22,20 μM BAP lub 4,65; 9,30; 23,25 μM KIN. Najlepsze rezultaty uzyskano, inokulując przecięte poprzecznie na pół kwiaty języczkowate na pożywkę z 4,65 μM KIN i 18,08 μM 2,4-D. Uzyskane wyniki mogą przyczynić się do zwiększenia prawdopodobieństwa separacji komponentów chimer w hodowli chryzantem.
Źródło:
Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 13-22
1644-0692
Pojawia się w:
Acta Scientiarum Polonorum. Hortorum Cultus
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of culture medium formulation on Pinus nigra somatic embryogenesis
Autorzy:
Salaj, T.
Fraterova, L.
Carach, M.
Salaj, J.
Powiązania:
https://bibliotekanauki.pl/articles/41577.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
culture medium
formulation
Pinus nigra
European black pine
histology
conifer
plant growth regulator
somatic embryo
somatic embryogenesis
Opis:
The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.
Źródło:
Dendrobiology; 2014, 71
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Regeneration of sorghum in cultures of isolated embryos
Autorzy:
Luwanska, A.
Dreger, M.
Mankowska, G.
Marszalek, M.
Wielgus, K.
Powiązania:
https://bibliotekanauki.pl/articles/951202.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
sorghum
regeneration
plant regeneration
organogenesis
somatic embryogenesis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Efektywnosc embriogenezy somatycznej w kulturze in vitro lnianki siewnej [Camelina sativa L.]
Autorzy:
Zandecka-Dziubak, J
Luczkiewicz, T.
Powiązania:
https://bibliotekanauki.pl/articles/832655.pdf
Data publikacji:
2000
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
Camelina sativa
kultury in vitro
zarodki somatyczne
embriogeneza somatyczna
lnicznik siewny
in vitro culture
somatic embryo
somatic embryogenesis
false flax
Źródło:
Rośliny Oleiste - Oilseed Crops; 2000, 21, 2; 615-620
1233-8273
Pojawia się w:
Rośliny Oleiste - Oilseed Crops
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Somatic embryogenesis of selected spruce species [Picea abies, P. omorika, P. pungens 'Glauca' and P. breweriana]
Autorzy:
Hazubska-Przybyl, T
Bojarczuk, K.
Powiązania:
https://bibliotekanauki.pl/articles/56682.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
somatic embryogenesis
spruce species
Picea abies
Picea omorika
Picea pungens
Picea breweriana
plant tissue
growth regulator
somatic embryo
nursery production
Opis:
Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g-1"1). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.
Źródło:
Acta Societatis Botanicorum Poloniae; 2008, 77, 3; 189-199
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Stimulation of somatic embryo growth and development in Picea spp. by polyethylene glycol
Autorzy:
Hazubska-Przybył, Teresa
Wawrzyniak, Mikołaj
Powiązania:
https://bibliotekanauki.pl/articles/956841.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
somatic embryogenesis
osmotic stress
germination
norway spruce
serbian spruce
Opis:
The objective of the presented study was to compare the effect of osmotic stress, induced by various concentrations of polyethylene glycol (PEG) MW 4000, on the development, maturation, and conversion of somatic embryos of Picea abies (model species) and P. omorika (endemic species) into somatic seedlings. Embryogenic tissue (ET) of P. abies (line A and B) and P. omorika were incubated on a maturation medium with 20 μM abscisic acid (ABA), 1 μM indole-3-butyric acid (IBA) and PEG 4000 at 0, 5, and 7.5%. After 5 weeks, the number of somatic embryos produced, and the number of cotyledonary somatic embryos per 1 gram of ET, was assessed. Cotyledonary somatic embryos were selected and transferred to a Margara germination medium for two months. Their growth, as measured by hypocotyl and radicle length, was evaluated after one month, and the somatic embryos were transferred to fresh medium. After the second month of incubation, the conversion frequency of germinated embryos into the seedlings was assessed. In general, ETs of both of the tested spruce species cultured on a maturation medium amended with 5% PEG 4000 exhibited a tendency to produce a higher total number of embryos, as well as a higher number of cotyledonary embryos. The B line of P. abies produced a significantly greater number of cotyledonary embryos, relative to the control (no PEG). PEG only stimulated hypocotyl growth in embryos of both lines (A and B) of P. abies during the germination stage. Somatic embryos of Picea omorika were characterized by poor growth of both hypocotyls and radicles, irrespective of PEG treatment. The addition of PEG 4000 to the maturation medium did not improve the conversion frequency of germinated somatic embryos into somatic seedlings in either spruce species after a month of incubation on Margara medium. The effect of PEG 4000 on somatic embryogenesis efficiency was more evident in the ET of the two P. abies lines, relative to the P. omorika ET line, during both the maturation and the germination stage. Results indicated that the P. omorika ET line was almost completely insensitive to all of the PEG concentrations tested.
Źródło:
Dendrobiology; 2017, 78; 168-178
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Induction of somatic embryogenesis in spruce: Picea omorika, P. pungens "Glauca", P. breweriana and P. abies
Autorzy:
Hazubska, T
Szczygiel, K.
Powiązania:
https://bibliotekanauki.pl/articles/41201.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
Norway spruce
Picea pungens
Serbian spruce
Picea abies
blue spruce
Picea omorika
clonal propagation
spruce
somatic embryogenesis
maturation
embryogenesis
Picea breweriana
somatic embryo
Opis:
The studies were carried out on seed explants (mature zygotic embryos) of four spruce species: Picea omorika (Pancić) Purk., P. pungens ‘Glauca' Beisnn., P. breweriana S. Watson and P. abies (L.) Karst. The explants required darkness during the induction of embryogenic tissue. Temporary exposure of explants to light did not increase their embryogenic capacity. It was observed that the temperature of 25°C was the optimal for embryogenic tissue induction from mature zygotic embryos of Picea omorika. Cold treatment at 4°C during one week improved embryogenic tissue initiation in the explants of P. pungens ‘Glauca'. Seed explants of Picea omorika produced embryogenic tissue on medium lacking glutamine. Only explants of Picea abies and P. omorika showed embryogenic tissue formation. For effective proliferation of embryogenic tissue in Picea abies and P. omorika it was necessary to supply the medium with 2,4-D (4.5-9 μM) and BA (2.25 μM). Two of three analized lines showed that ABA promote somatic embryo maturation, at the ABA concentration 20 μM in the medium.
Źródło:
Dendrobiology; 2003, 50
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of phytosulfokine on embryo development in protoplast cultures of Daucus carota subspecies – histological analysis
Autorzy:
Mackowska, K.
Tuleja, M.
Grzebelus, E.
Powiązania:
https://bibliotekanauki.pl/articles/951307.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
somatic embryogenesis
embryonic development
phytosulphokine
protoplast culture
Daucus carota
histological analysis
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
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