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Wyświetlanie 1-15 z 112
Tytuł:
Improved method of isolation of total nucleic acids from hop plants and grapevine before the RT-PCR by addition of polyvinylpolypyrrolidone
Usprawniona metoda izolacji calkowitych kwasow nukleinowych z chmielu i winorosli przez RT-PCR przez dodanie poliwinylopolipirolidonu
Autorzy:
Cajza, M
Folkman, W.
Powiązania:
https://bibliotekanauki.pl/articles/65582.pdf
Data publikacji:
2003
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
isolation
total nucleic acid
nucleic acid
hop plant
grapevine
RT-PCR method zob.reverse transcription polymerase chain reaction
addition
polyvinylpolypyrrolidone
reverse transcription polymerase chain reaction
polymerase chain reaction
Źródło:
Journal of Plant Protection Research; 2003, 43, 4; 375-380
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) in the analysis of single nucleotide polymorphisms (SNPs)
Autorzy:
Tarach, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/1830648.pdf
Data publikacji:
2021-09-29
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
nucleotide polymorphisms
DNA analysis
polymerase chain reaction
Opis:
Polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR) is a technique used to identify single nucleotide polymorphisms (SNPs) based on the recognition of restriction sites by restriction enzymes. RFLP-PCR is an easy-to-perform and inexpensive tool for initial analysis of SNPs potentially associated with some monogenic diseases, as well as in genotyping, genetic mapping, lineage screening, forensics and ancient DNA analysis. The RFLP-PCR method employs four steps: (1) isolation of genetic material and PCR; (2) restriction digestion of amplicons; (3) electrophoresis of digested fragments; and (4) visualisation. Despite its obsolescence and the presence of high-throughput DNA analysis techniques, it is still applied in the analysis of SNPs associated with disease entities and in the analysis of genetic variation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). RFLP-PCR is a low-cost and low-throughput research method allowing for the analysis of SNPs in the absence of specialised equipment, and it is useful when there is a limited budget.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2021, 17; 48-53
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism in Syringa rDNA regions assessed by PCR technique
Autorzy:
Smolik, M.
Andrys, D.
Franas, A.
Krupa-Malkiewicz, M.
Malinowska, K.
Powiązania:
https://bibliotekanauki.pl/articles/41641.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
polymorphism
lilac
Syringa
rDNA region
polymerase chain reaction
Opis:
The Syringa genus is characterizedby a multiplicity of forms. Its chief asset is the ornamental value of thousands of accessions, species or hybrids. From a phylogenetic point of view the genus is difficult in an explicit classification due to its frequently complex genome. The aim of this study was to determine the possibility for the identification of genotypic diversity and genetic relationships in the nrDNA sequence of some selected Syringa accessions – part of a collection of the Dendrological Garden in Przelewice (Poland). For this purpose, the PCR technique together with a combination of various ‘universal’ primers designed for the nrDNA sequence analysis were employed. Fourteen Syringa accessions: Syringa × chinensis Willd., S. × prestoniae Mc Kelv., S. × prestoniae ‘Telimena’, S. × prestoniae ‘Jaga’, S. × prestoniae ‘Basia’, S. meyeri ‘Palibin’, S. vulgaris ‘Miss Ellen Willmott’, S. vulgaris, S. vulgaris ‘Jules Simon’, S. vulgaris ‘Katherine Havemeyer’, S. vulgaris ‘Krasawica Moskvy’, S. vulgaris ‘Mirabeau’, S. vulgaris ‘Madame Lemoine’ and S. vulgaris ‘Niebo Moskvy’ made up the research material. In the conducted amplifications, genetic profiles were obtained for 14 combinations among the 25 combinations of different pairs of primers used. The nrDNA templates coding the small subunit (SSU), 5.8S subunit andITS1, ITS2 andIGS sequences were amplified. In PCR reactions a total of 33 PCR products were generated, of which 21 (64%) products were polymorphic, 6 (18%) monomorphic and6 (18%) were genotype-specific. For the lilac accessions examined246 amplicons were generated from ~230 to ~1100 bp in length. The analysis of both the dendrogram and the genetic similarity matrix revealedlow diversity between the examinedaccessions. For most they rangedfrom 70 to 80%, andthe greatest diversity (87%) was foundbetween the S. × prestoniae: ‘Basia’ and‘Telimena’ accessions, while the lowest (57%) was observed between S. vulgaris ‘Katherine Havermeyer’ and S. × chinensis.
Źródło:
Dendrobiology; 2010, 64
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular diagnostics of Sarcocystis spp. infections
Autorzy:
Stojecki, K.
Karamon, J.
Sroka, J.
Cencek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2088002.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
molecular diagnostics
Sarcocystis
infection
sarcocystosis
polymerase chain reaction
Opis:
Protozoa of the genus Sarcocystis (phylum Apicomplexa, family Sarcocystidae) is one of the most common parasites affecting animals. Interspecies diagnostic of Sarcocystis genus was based on electron microscopy for many years. Because of absence of visible differences between species with reachable magnifications, light microscopy is useless. In many cases serological diagnostic method have lack of sensitivity. A variety of molecular methods have been developed and used to detect and identify Sarcocystis spp. and to assess the genetic diversity among this protozoan from different population/hosts. Nowadays, molecular diagnostic is the common, time/cost effective method used all over the world to interspecies differentiation.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Antimicrobial activities and phylogenetic study of Erythrina senegalensis DC (Fabaceae) seed lectin
Autorzy:
Enoma, Samuel
Adewole, Taiwo S.
Agunbiade, Titilayo O.
Kuku, Adenike
Powiązania:
https://bibliotekanauki.pl/articles/16672367.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
antimicrobial
Erythrina senegalensis
lectin
phylogenetic analysis
polymerase chain reaction
Opis:
Erythrina senegalensis (Fabaceae) have been traditionally used in the treatment of microbial ailments, and the specific agent mediating its efficacy has been investigated in several studies. In this study, the antimicrobial activity of purified E. senegalensis lectin (ESL) was analyzed. The phylogenetic relationship of the gene encoding lectin with other legume lectins was also established to investigate their evolutionary relationship via comparative genomics. Antimicrobial activity of ESL against selected pathogenic bacteria and fungi isolates was evaluated by the agar well diffusion method, using fluconazole (1 mg/ml) and streptomycin (1 mg/ml) as positive controls for fungi and bacteria sensitivity, respectively. Potent antimicrobial activity of ESL against Erwinia carotovora, Pseudomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Aspergillus niger, Penicillium camemberti, and Scopulariopsis brevicaulis was observed, with inhibition zones ranging from 18 to 24 mm. Minimum inhibitory concentrations of ESL ranged between 50 and 400 μg/ml. Primer-directed polymerase chain reaction of E. senegalensis genomic DNA detected a 465-bp lectin gene with an open reading frame encoding a 134-amino acid polypeptide. The obtained nucleotide sequence of the ESL gene shared high sequence homology: 100, 100, and 98.18% with Erythrina crista-galli, Erythrina corallodendron, and Erythrina variegata lectin genes, respectively, suggesting that the divergence of Erythrina lectins might follow species evolution. This study concluded that ESL could be used to develop lectin-based antimicrobials, which could find applications in the agricultural and health sectors.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2023, 104, 1; 21-32
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
DNA isolation from Cryptosporidium oocysts directly from stool samples for diagnostic goals using PCR
Autorzy:
Sulima, P.
Powiązania:
https://bibliotekanauki.pl/articles/840154.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
diagnostic goal
stool
polymerase chain reaction
Cryptosporidium
DNA
oocyst
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of bovine leucocyte adhesion deficiency [BLAD] carriers using a new PCR test
Autorzy:
Kaminski, S
Czarnik, U
Powiązania:
https://bibliotekanauki.pl/articles/2046599.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cattle
bovine leucocyte adhesion deficiency
polymerase chain reaction
bacterial infection
Opis:
In this report we demonstrate a simple, effective and reliable diagnostic test of BLAD carrier detection based on specific PCR amplification of a 367 bp CD18 gene fragment and RFLP analysis using Taq I restriction enzyme. In a non-random population of 220 animals we found 48 BLAD carriers. Within the amplified PCR fragment an unknown intron sequence of 159 bp was identified.
Źródło:
Journal of Applied Genetics; 1997, 38, 1; 51-55
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vegetable oil plant wastewater treatment by bacterial isolates : a study in the city of Hila, Iraq
Autorzy:
Salim, Hanan Kareem
Al-Ahmed, Suad Ghali Kadhim
Powiązania:
https://bibliotekanauki.pl/articles/2048496.pdf
Data publikacji:
2021
Wydawca:
Instytut Technologiczno-Przyrodniczy
Tematy:
biotreatment
polymerase chain reaction
PCR
sewage
vegetable oil plant
wastewater
Opis:
The present study was to reflect the use of some bacteria in the treatment and removal of pollutants in three selected wastewater sites, including a vegetable oil plant (viz. Al-Etihad Food Industries), the main wastewater treatment station in the city of Hila, and Al-Hila River water from October 2019 to January 2020. The bacterial isolates identified in these three sites were Klebsiella pneumoniae, Escherichia coli, Enterobacteria cloacae, Pseudomonas aeruginosa, Thalasobacillus devorans, Acinetobacter baumannii, and Bacillus subtilis. The molecular study of the bacterial isolates involved the detection of bacterial genera using the polymerase chain reaction (PCR). The results showed that water had a variable nature, depending on the substances in it. It recorded varying chemical and physical property values, ranging between 6.36 and 7.82 for pH and from 2500 to 7100 mg∙dm-3 for total alkalinity. Additional values were 713–2051 μS∙cm-1 for electrical conductivity (EC), 5.90–9.80 mg∙dm-3 for chemical oxygen demand (COD), and 480–960 mg∙dm-3 for total hardness. The given values were also 0.20–0.65 μg∙dm-3, 0.03-0.23 μg∙dm-3, and 0–107 mg∙dm-3 for nitrite (NO2), phosphate (PO4) oils, respectively.
Źródło:
Journal of Water and Land Development; 2021, 51; 163-167
1429-7426
2083-4535
Pojawia się w:
Journal of Water and Land Development
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hantavirus RNA not detected in Ixodes ricinus ticks
Autorzy:
Wojcik-Fatla, A.
Zajac, V.
Knap, J.P.
Dutkiewicz, J.
Powiązania:
https://bibliotekanauki.pl/articles/49890.pdf
Data publikacji:
2011
Wydawca:
Instytut Medycyny Wsi
Tematy:
hantavirus
RNA
Ixodes ricinus
tick
epidemiology
polymerase chain reaction
Polska
Źródło:
Annals of Agricultural and Environmental Medicine; 2011, 18, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
Autorzy:
Woloszyn, A.
Kotlowski, R.
Powiązania:
https://bibliotekanauki.pl/articles/875623.pdf
Data publikacji:
2017
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
identification method
gene encoding
amatoxin
phallotoxin
poisonous mushroom
polymerase chain reaction
Opis:
Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic peptides - amanitins and phallotoxins present in the most dangerous poisonous mushrooms. Material and Methods. Degenerate primers in the PCR were designed on the basis of amanitins (n=13) and phallotoxins (n=5) genes in 18 species of poisonous mushrooms deposited to Genbank of the National Center for Biotechnology Information. Results. The specificity of the PCR assays was confirmed against 9 species of edible mushrooms, death cap - Amanita phalloides and panther cap - Amanita pantherina. Conclusions. Designed two couples of PCR-primers specific to amanitins and phallotoxins genes can be recommended for detection of Amanita phalloides and other mushroom species producing hepatotoxic cyclic peptides - amanitins and phallotoxins.
Wprowadzenie. Ponieważ, obecnie znane diagnostyczne cele molekularne w genomach trujących grzybów kapeluszowych amplifikowane metodą PCR mają swoje odpowiedniki u grzybów jadalnych, istnieje potrzeba zastosowania specyficznych sekwencji starterowych wobec amanityn i fallotoksyn, występujących jedynie u grzybów trujących. Cel. Celem prowadzonych badań było sprawdzenie przydatności sekwencji starterowych do reakcji PCR specyficznych wobec wszystkich aktualnie poznanych genów kodujących hepatotoksyczne cykliczne peptydy - amanityny oraz fallotoksyny trujących grzybów kapeluszowych. Materiał i Metody. Sekwencje oligonokleotydowe starterów do reakcji PCR zaprojektowane zostały w oparciu o zdeponowane w Genbanku geny amanityn (n=13) oraz fallotoksyn (n=5). Wyniki. Specyficzność opracowanych testów PCR potwierdzono wobec 9 gatunków grzybów jadalnych oraz muchomora sromotnikowego - Amanita phalloides, jak i muchomora plamistego - Amanita pantherina. Wnioski. Zastosowane sekwencje starterowe do wykrywania genów kodujących amanityny i fallotoksyny metodą PCR, mogą być wykorzystane do wykrywania muchomora sromotnikowego oraz innych gatunków zdolnych do syntezy amanityn oraz fallotoksyn.
Źródło:
Roczniki Państwowego Zakładu Higieny; 2017, 68, 3
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation of the DNA fragment reflecting the open reading frame II of the I-18 C gene of Chironomus tentans by the polymerase chain reaction. IV. The product of the applied technologies
Autorzy:
Borowicz, B P
Powiązania:
https://bibliotekanauki.pl/articles/65915.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
I-18 C gene
Chironomus tentans
polymerase chain reaction
DNA fragment
Opis:
The Polymerase Chain Reaction and other molecular technologies were applied to isolate the DNA fragment reflecting the Open Reading Frame II of the I-18 C gene. This research object was realized and the importance of this result has been discussed in view of the mechanisms of the regulation of gene expression.
Reakcja Łańcuchowa Polimerazy i inne technologie molekularne zostały zastosowane w celu izolacji fragmentu DNA odpowiadającego Otwartej Ramie Odczytu II genu I-18 C. Cel badawczy został zrealizowany i omówiono znaczenie tego wyniku w świetle mechanizmów regulacji ekspresji genu.
Źródło:
Journal of Plant Protection Research; 1997, 37, 1-2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Segmented hybridization probes: modulating target affinity and base pairing selectivity
Autorzy:
Egetenmeyer, S.
Geiger, E.
Richert, C.
Powiązania:
https://bibliotekanauki.pl/articles/81071.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
oligonucleotide
DNA
RNA
hybridization
base pairing
molecular biology
polymerase chain reaction
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2012, 93, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polymorphism of DNA in nematodes of genus Trichinella Railliet, 1895 according to the data of polymerase chain reaction
Autorzy:
Shendrick, A.
Benedictov, I.
Bessonov, A.
Powiązania:
https://bibliotekanauki.pl/articles/838885.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
polymorphism
Trichinella pseudospiralis
Trichinella
nematode
polymerase chain reaction
DNA
Trichinella spiralis
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Virulence and antibiotic resistance of Escherichia coli isolated from rooks
Autorzy:
Kmet, V.
Drugdova, Z.
Kmetova, M.
Stanko, M.
Powiązania:
https://bibliotekanauki.pl/articles/51141.pdf
Data publikacji:
2013
Wydawca:
Instytut Medycyny Wsi
Tematy:
virulence
antibiotic resistance
Escherichia coli
isolation
rook
polymerase chain reaction
DNA microarray
Opis:
With regard to antibiotic resistance studies in various model animals in the urban environment, the presented study focused on the rook, many behavioural and ecological aspects of which are important from an epidemiological point of view. A total of 130 Escherichia coli strains isolated from rook faeces during a two-year period (2011–2012) were investigated for antibiotic resistance and virulence. Resistance to ampicillin (60%) and streptomycin (40%) were the most frequent, followed by resistance to fluoroquinolones (ciprofloxacin-22% and enrofloxacin-24%), tetracycline (18%), cotrimoxazol (17%) and florfenicol (14%). Ceftiofur resistance occured in 10.7 % of strains and cefquinom resistance in 1.5 % of strains. Twenty-five E.coli strains with a higher level of MICs of cephalosporins (over 2mg/L of ceftazidime and ceftriaxon) and fluoroquinolones were selected for detection of betalactamase genes (CTX-M, CMY), plasmid-mediated quinolone resistance qnrS, integrase 1, and for APEC (avian pathogenic E.coli) virulence factors (iutA, cvaC, iss, tsh, ibeA, papC, kpsII). Genes of CTX-M1, CMY-2, integrase 1, papC, cvaC, iutA were detected in one strain of E.coli, and qnrS, integrase 1, iss, cvaC, tsh were detected in another E.coli. DNA microarray revealed the absence of verotoxin and enterotoxin genes and pathogenicity islands. The results show that rooks can serve as a reservoir of antibiotic-resistant E. coli with avian pathogenic virulence factors for the human population, and potentially transmit such E.coli over long distances.
Źródło:
Annals of Agricultural and Environmental Medicine; 2013, 20, 2
1232-1966
Pojawia się w:
Annals of Agricultural and Environmental Medicine
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of nivalenol and deoxynivalenol chemotypes produced by Fusarium graminearum species complex isolated from barley in Iran using specific PCR assays
Autorzy:
Chehri, K.
Godini, R.
Powiązania:
https://bibliotekanauki.pl/articles/66014.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
detection
nivalenol
deoxynivalenol
Fusarium graminearum
isolation
polymerase chain reaction
barley
trichothecene
Iran
Opis:
In order to identify trichothecenes chemotypes produced by Fusarium graminearum species complex (FGSC) isolated from barley, 68 barley samples were collected from markets in Kermanshah and Hamedan provinces, Iran. Thirty-one Fusarium isolates were obtained from grains and morphologically classified into three species FGSC (14), F. equiseti (9), and F. proliferatum (8). The identification of the members of FGSC was confirmed molecularly using Fg16F/Fg16R primers. Fusarium asiaticum isolates (4) were distinguished from other FGSC using Fg6CTPSf177/Fg16R primers. Polymerase chain reaction-based (PCRbased) detection of mycotoxin-synthesis-pathway gene was also used to determine the potential of the analysed strains to produce deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-AcDON), 3-acetyldeoxynivalenol (3-AcDON), and nivalenol (NIV). Of 14 tested isolates, 10 and 4 isolates belonged to DON and NIV chemotype, respectively. Also, the results of DON chemotype survey using specific primers MinusTri7F/R and Tri315F/R showed 1 and 9 isolates produced 3-AcDON and 15-AcDON, respectively. These results show that DON was the most common chemotype in western Iran. To our knowledge, this is the first report on 15-AcDON, 3-AcDON, and NIV isolated from barley in Iran.
Źródło:
Journal of Plant Protection Research; 2017, 57, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 112

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