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Wyświetlanie 1-14 z 14
Tytuł:
Biotechnology of temperate fruit trees and grapevines
Autorzy:
Laimer, Margit
Mendonça, Duarte
Maghuly, Fatemeh
Marzban, Gorji
Leopold, Stephan
Khan, Mahmood
Balla, Ildiko
Katinger, Hermann
Powiązania:
https://bibliotekanauki.pl/articles/1041374.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
plant biotechnology
patogen detection
molecular markers
Opis:
Challenges concerning fruit trees and grapevines as long lived woody perennial crops require adapted biotechnological approaches, if solutions are to be found within a reasonable time frame. These challenges are represented by the need for correct identification of genetic resources, with the foreseen use either in conservation or in breeding programmes. Molecular markers provide most accurate information and will be the major solution for questions about plant breeders rights. Providing healthy planting material and rapid detection of newly introduced pathogens by reliable methods involving serological and molecular biological tools will be a future challenge of increases importance, given the fact that plant material travels freely in the entire European Union. But also new breeding goals and transgenic solutions are part of the biotechnological benefits, e.g. resistance against biotic and abiotic stress factors, modified growth habits, modified nutritional properties and altered processing and storage qualities. The successful characterization of transgenic grapevines and stone fruit trees carrying genes of viral origin in different vectors constructed under ecological consideration, will be presented. Beyond technical feasibility, efficiency of resistance, environmental safety and Intellectual Property Rights, also public acceptance needs consideration and has been addressed in a specific project. The molecular determination of internal quality parameters of food can also be addressed by the use of biotechnological tools. Patient independent detection tools for apple allergens have been developed and should allow to compare fruits from different production systems, sites, and genotypes for their content of health threatening compounds.
Źródło:
Acta Biochimica Polonica; 2005, 52, 3; 673-678
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of Potato Resistance to Synchytrium Endobioticum
Autorzy:
Przetakiewicz, Jarosław
Plich, Jarosław
Powiązania:
https://bibliotekanauki.pl/articles/2199702.pdf
Data publikacji:
2017-12-20
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
potato wart disease
resistance
molecular markers
Opis:
In Poland the Plant Breeding and Acclimatization Institute - National Research Institute (IHAR-PIB) is responsible for officially assessing the resistance of potato breeding lines and cultivars to Synchytrium endobi-oticum. In the official assessment of wart resistance the modified Glynne-Lemmerzahl method is used. A full cycle of assessment of the wart disease resistance requires 42 - 45 tubers per cultivar/breeding line. Forty two tubers are used in laboratory tests. To complete the laboratory tests, another 10 tubers are inoculated with winter sporangia of the fungus, using ring test. The final results are available after 3 years of investigation. If necessary, the full cycle of resistance tests to S. endobioticum can be performed during one year on 15 tubers in each of the 3 replications (45 tubers in total).Molecular verification of potato genotypes resistance to pathotype 1(D1) is possible with the use of SCAR marker Nl25-1400. Nevertheless, an official phenotypical assessment of advanced breeding lines, as the final verification of their resistance, is required.
Źródło:
Plant Breeding and Seed Science; 2017, 76; 37-43
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of circulating breast cancer cells in peripheral blood by a two-marker reverse transcriptase-polymerase chain reaction assay.
Autorzy:
Fabisiewicz, Anna
Kulik, Jadwiga
Kober, Paulina
Brewczyńska, Elżbieta
Pieńkowski, Tadeusz
Siedlecki, Janusz
Powiązania:
https://bibliotekanauki.pl/articles/1041553.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
RT-PCR
breast cancer
molecular markers
Opis:
The aim of this study was to use a two-marker assay for the detection of breast cancer cells circulating in patients' blood. We have applied a PCR-based methodology to follow up the possibility of the development of metastatic disease in stage I and II patients who had undergone curative surgery. Since the number of circulating cancer cells in peripheral blood is very low, the technique for their detection needs to be not only highly sensitive, but also very specific. The reverse transcriptase-polymerase chain reaction (RT-PCR) technique may improve the sensitivity of breast cancer cell detection up to only a few cells per one million. The principle of the RT-PCR assay is to amplify a messenger RNA characteristic for breast epithelial cells in a blood sample. Since we do not expect such cells to be circulating in peripheral blood of healthy subjects, detection of the characteristic mRNA should indicate the presence of circulating breast cancer cells. We analyzed the usefulness of three mRNA markers: cytokeratin 19 (CK19), mammaglobin (hMAM) and β subunit of human chorionic gonadotropin (β-hCG) for this test. Blood samples (112) were obtained from 55 patients, in stages I and II, with or without metastasis to regional lymph nodes (N0 or N1). We found that a two-marker assay increases the sensitivity of detection of breast cancer cells in comparison with a single-marker one. Combination of two tumor-specific mRNA markers, hMAM/CK19 or β-hCG/CK19, allowed the detection of circulating breast cancer cells in 65% of N1 patients and 38% of N0 patients. By comparison, the combination hMAM/β-hCG allowed the detection of circulating breast cancer cells in the blood of 68% of N1 patients and 46% of N0 patients. Addition of the third marker did not significantly increase the detection sensitivity.
Źródło:
Acta Biochimica Polonica; 2004, 51, 3; 747-755
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of RAPD, ISSR and SSR markers in assessing genetic diversity among rye*(Secale cereale L.) inbred lines
Autorzy:
Myśków, Beata
Milczarski, Paweł
Masojc, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/2199589.pdf
Data publikacji:
2010-12-01
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
dendrogram
genetic diversity
molecular markers
rye
Opis:
Forty eight inbred lines of winter rye, of various origin and pedigree, were analysed using 19 RAPD (random amplified polymorphic DNA) primers, 8 ISSR (inter-simple sequence repeats) primers and 13 SSR (simple sequence repeats) primer pairs. On the basis of particular marker types, there were created three separate dendrograms and one combined similarity tree, prepared on account of the whole data. Correlation coefficients for individual technique based on genetic similarity matrices were not significant. By comparing the GS data obtained on the basis of singular methods with collective matrix, it was observed that the highest correlation rate was for ISSR method (r=0.68). The utility of each marker technique was compared by using marker index MI. Diversity detecting index (DDI) was suggested in the paper, which may prove helpful in planning and comparing researches on phenetic relationships...
Źródło:
Plant Breeding and Seed Science; 2010, 62; 107-116
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Current and potential oncomarkers in diagnosing breast cancer
Obecnie używane i potencjalne markery nowotworowe w diagnostyce raka piersi
Autorzy:
Bilecová-Rabajdová, M.
Urban, P.
Grešová, A.
Varga, J.
Gregová, K.
Stupák, M.
Mareková, M.
Powiązania:
https://bibliotekanauki.pl/articles/271594.pdf
Data publikacji:
2012
Wydawca:
Górnośląska Wyższa Szkoła Pedagogiczna im. Kardynała Augusta Hlonda
Tematy:
rak piersi
diagnostyka
markery molekularne
markery nowotworowe TVMs
breast cancer
diagnostics
molecular markers
tumour vascular markers
Opis:
Breast cancer is a very serious disease from both an economical and social point of view, because of its high incidence and mortality, as well as its prevalence. Genetic and epigenetic changes as well as the resultant deregulation of mechanisms that affect cellular processes are reflected in the final stage, like significant variability in phenotype. These changes have multiple factors in clinical, morphological but also molecular terms, and they greatly affect the possibility of accurate diagnosis of breast cancer in its early stages. Since no combination of clinical examination, mammography and ultrasonography together with CT and MRI can achieve 100% sensitivity, clinical practice is supported with diagnostic methods based on the monitoring of tumour markers with the emphasis on molecular markers. This review describes the most common molecular markers already used for earlier clinical diagnostics and also proposes new possible markers from the tumour vascular markers group. Any new oncomarkers to improve diagnostic sensitivity and specificity will be of great benefit, and in clinical practice, they may provide an opportunity for early diagnostic and therapeutic intervention, and subsequently better prognosis for the patient as well.
Rak piersi jest bardzo poważną chorobą, zarówno z punktu widzenia ekonomicznego jak i społecznego, ze względu na dużą zachorowalność i umieralność, a także na zakres jego występowania. Zmiany genetyczne i epigenetyczne, jak również wynikająca z nich deregulacja mechanizmów, które wpływają na procesy komórkowe są odzwierciedlone w ostatnim stadium choroby, jak znaczna zmienność fenotypu. Zmiany te mają wiele przyczyn z punktu widzenia klinicznego, morfologicznego, ale również molekularnego oraz znacznie wpływają na możliwość dokładnego rozpoznania raka piersi w jego wczesnych fazach. Ponieważ żadna kombinacja badań klinicznych, mammografii i USG wraz z tomografią komputerową i obrazowaniem magnetyczno-rezonansowym nie może osiągnąć 100% czułości, praktyka kliniczna jest wspierana metodami diagnostycznymi opartymi na monitoringu markerów nowotworowych, ze szczególnym uwzględnieniem markerów molekularnych. Niniejsza recenzja opisuje najbardziej typowe markery molekularne wykorzystywane we wczesnej diagnostyce klinicznej, a także proponuje nowe potencjalne markery z grupy markerów nowotworowych TVMs . Wszelkie nowe markery nowotworowe poprawiające czułość i dokładność diagnostyki będą bardzo pomocne, a w praktyce klinicznej mogą pozwolić na wczesną diagnostykę i leczenie, co polepszy także rokowania pacjenta.
Źródło:
Journal of Ecology and Health; 2012, R. 16, nr 3, 3; 138-143
2082-2634
Pojawia się w:
Journal of Ecology and Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Application of the High Resolution Melting analysis for genetic mapping of Sequence Tagged Site markers in narrow-leafed lupin (Lupinus angustifolius L.)
Autorzy:
Kamel, Katarzyna
Kroc, Magdalena
Święcicki, Wojciech
Powiązania:
https://bibliotekanauki.pl/articles/1039001.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
HRM
molecular markers
genotyping
narrow-leafed lupin
Opis:
Sequence tagged site (STS) markers are valuable tools for genetic and physical mapping that can be successfully used in comparative analyses among related species. Current challenges for molecular markers genotyping in plants include the lack of fast, sensitive and inexpensive methods suitable for sequence variant detection. In contrast, high resolution melting (HRM) is a simple and high-throughput assay, which has been widely applied in sequence polymorphism identification as well as in the studies of genetic variability and genotyping. The present study is the first attempt to use the HRM analysis to genotype STS markers in narrow-leafed lupin (Lupinus angustifolius L.). The sensitivity and utility of this method was confirmed by the sequence polymorphism detection based on melting curve profiles in the parental genotypes and progeny of the narrow-leafed lupin mapping population. Application of different approaches, including amplicon size and a simulated heterozygote analysis, has allowed for successful genetic mapping of 16 new STS markers in the narrow-leafed lupin genome.
Źródło:
Acta Biochimica Polonica; 2015, 62, 3; 533-540
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Fast and cheap identification of elite aspen clones in the North-West of Russia using ISSR markers
Autorzy:
Zhigunov, Anatoly V.
Shabunin, Dmitrii A.
Butenko, Olesia Yu.
Lebedeva, Marina V.
Powiązania:
https://bibliotekanauki.pl/articles/2045732.pdf
Data publikacji:
2018
Wydawca:
Instytut Badawczy Leśnictwa
Tematy:
aspen
elite clones
molecular markers
ISSR
clonal plantations
Opis:
In 2001–2006, several experimental aspen plantations were established in the North-West of Russia (Leningrad region). Three in vitro propagated elite aspen (Populus tremula L.) clones from the Kostroma Forest Research Station were used as the planting stock for plantations. The planting plans of some experimental plantations were lost, which made it impossible to identify the genetic lineages. 13-years old unknown aspen clones demonstrated prominent growth rates, and reliably overtook natural aspen coppice. ISSR markers were used for fast and cheap restoring of the missing planting plan of the experimental aspen plantation under study; as a result, progenies of three elite aspen clones were recognized. The best fast-growing and stem rot resistant aspen clones was identified and called “Kostroma”.
Źródło:
Folia Forestalia Polonica. Series A . Forestry; 2018, 60, 4; 207-213
0071-6677
Pojawia się w:
Folia Forestalia Polonica. Series A . Forestry
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interval mapping of genes controlling growth of rye plants
Autorzy:
Milczarski, Paweł
Masojć, Piotr
Powiązania:
https://bibliotekanauki.pl/articles/2198924.pdf
Data publikacji:
2003-12-21
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
QTLs
growth
genetic map
molecular markers
Secale cereale L.
Opis:
The F2-type population derived from the cross between DS2 and RXL1O inbred lines was used for interval mapping of five growth related traits i.e. plant height, spike length, thousand grain weight, kernel length and kernel thickness. Scanning of the whole 1,140 cM length of rye genetic map consisting of 286 marker loci revealed the existence of 6 regions containing QTL5 on chromosomes 1R-5R. Plant height was strongly affected by 1-3 linked dwarfing genes from a distal region of the chromosome 5RL and by 1 gene on the chromosome 3RL, tightly linked to a marker loci Xpsr4 75. These same genes regulated also thousand grain weight and kernel length and thickness. Spike length was determined only by the QTL from chromosome 5RL. In addition a single QTL from chromosome 2R affecting thousand grain weight and kernel thickness was identified, near the molecular marker locus Xrsq8OS. 1. Kernel length and kernel thickness were additionally controlled by QTL5 on chromosomes 2R and 1R and 4R, respectively.
Źródło:
Plant Breeding and Seed Science; 2003, 48; 135-142
1429-3862
2083-599X
Pojawia się w:
Plant Breeding and Seed Science
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Analysis of molecular markers as IL-12, IL-22 and IFN-γ in correlation with a clinical course in patients with psoriasis
Autorzy:
Kutwin, Magdalena
Migdalska-Sęk, Monika
Brzeziańska-Lasota, Ewa
Zelga, Piotr
Woźniacka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/2116535.pdf
Data publikacji:
2020-09-17
Wydawca:
Instytut Medycyny Pracy im. prof. dra Jerzego Nofera w Łodzi
Tematy:
psoriasis
disease activity
proinflammatory cytokines
skin disease
immunology
molecular markers
Opis:
ObjectivesAs a chronic, recurrent, immunologically mediated systemic disease and a common cause of dermatological problems, psoriasis is often a subject of scientific research. Skin changes located on the hands can cause difficulties and limitations in the performance of professional activities, especially manual ones. The main role in pathogenesis is played by immunological factors – improper functioning of the components of the immune system, among others, T lymphocytes and cytokines like interleukin-12 (IL-12), interleukin-22 (IL-22) and interferon gamma (IFN-γ).Material and MethodsThe obtained tissue and blood were destined for RNA isolation. The RNA was then subjected to a reverse transcription reaction. The relative gene expression level was evaluated by the real-time polymerase chain reaction for IL-12B, IL-22 and IFN-γ genes, and presented as the relative quantification (RQ) value, relative to the reference gene GAPDH. In addition, a correlation analysis of the expression level of selected genes with the clinical course of the disease, as assessed by the Psoriasis Area and Severity Index (PASI), the Body Surface Area (BSA) and the Dermatology Life Quality Index (DLQI) scores was performed.ResultsStatistical analysis confirmed a significant increase in RQ values for IL-12B, IL-22 and IFN-γ in the group of psoriatic patients vs. the control group. A positive correlation was also found between BSA and PASI and RQ for the IL-12B gene.ConclusionsIncreased expression levels of IL-12B, IL-22 and IFN-γ genes in psoriatic skin confirm that selected cytokines play an important role in the initiation and sustenance of psoriasis.
Źródło:
International Journal of Occupational Medicine and Environmental Health; 2020, 33, 5; 635-647
1232-1087
1896-494X
Pojawia się w:
International Journal of Occupational Medicine and Environmental Health
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
β-Glucan-Mediated Alleviation of NaCl Stress in Ocimum basilicum L. in Relation to the Response of Antioxidant Enzymes and Assessment DNA Marker
Autorzy:
Alhasnawi, Arshad Naji
Powiązania:
https://bibliotekanauki.pl/articles/124951.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
NaCl-stress
beta-glucan
antioxidants
molecular markers
DNA variations
genetic stability
Opis:
Salinity is one of the most important abiotic stresses which can negatively affect the plant metabolic processes in the world. This can impact the plant production, either for economic or sustenance benefits. The salinity stress can cause many physiological and biochemical changes in the plants. β-glucans are important polysaccharides, which are present in the cell walls of various cereal grains. They protect the plant responses and occur in plant suspensions. In this study, the researchers attempted to investigate various physiological mechanisms and determine the role of the β-glucans in the NaCl-mediated stress conditions on the Ocimum basilicum L. seedlings. For this purpose, they carried out an experiment for assessing various shoot and root parameters along with the antioxidant enzyme activities, proline levels and the ISSR markers. When the seedlings were exposed to the NaCl stress conditions, they showed a significant decrease in the growth parameters and an increase in the antioxidant and proline levels compared to the control seedlings grown under normal saline conditions. On the other hand, the β-glucantreated seeds, when grown under the saline stress conditions, showed better growth parameters as well as high antioxidant enzyme activities and proline levels, compared to the control and NaCl-treated plants. Furthermore, a PCR analysis was carried out using the ISSR-marker technology, which could help in evaluating the DNA fingerprints and genetic variations in the plants. The results indicated that the exogenous application of the β-glucans could protect the antioxidant enzyme activities and protect the plants against the salinity stresses, without affecting the DNA-markers without affecting the genetic variations and could be a better choice for use in DNA-markers.
Źródło:
Journal of Ecological Engineering; 2019, 20, 8; 90-99
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Metody oparte o amplifikację DNA techniką PCR wykorzystywane w ocenie bioróżnorodności mikroorganizmów glebowych
Methods based on DNA PCR-amplification for evaluation of the soil microbial diversity
Autorzy:
Łyszcz, Małgorzata
Gałązka, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1034148.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Przyrodników im. Kopernika
Tematy:
soil microorganism
genetic diversity
molecular markers
PCR based methods
ARDRA
PCR-RFLP
RAPD
REP-PCR
TRFLP
Opis:
Mikroorganizmy glebowe, pod względem cech genomowych i fenotypowych, stanowią wysoce zróżnicowaną grupę organizmów żywych. Z powodu tak dużej różnorodności ważne jest dobranie odpowiednich metod, dających największy stopień różnicowania mikroorganizmów. Narzędziami umożliwiającym analizę zmienności genetycznej mikroorganizmów są techniki genetyczne, a wśród nich jedną z najważniejszych jest łańcuchowa reakcja polimerazy, czyli PCR (Polymerase Chain Reaction), technika opracowana w latach 1980. Niniejsza praca stanowi przegląd podstawowych zagadnień dotyczących badania zmienności genetycznej mikroorganizmów glebowych w oparciu o markery molekularne z wykorzystaniem technik bazujących na reakcji PCR tj. PCR-RFLP, TRFLP, ARDRA, RAPD.
Soil microorganisms represent a highly diverse group of living organisms in terms of genomic and phenotypic characteristics. Due to such a large diversity, it is important to select appropriate identification methods which would secure its most complete determination. Genetic techniques are proper tools of choice for analyzing genetic variability of microorganism, the most important of which is the polymerase chain reaction (PCR), developed in the 1980s. This work presents an overview of the basic issues concerning studies on genetic variability of soil microorganisms with help of molecular markers and application of PCR techniques such as PCR-RFLP, TRFLP, ARDRA, RAPD.
Źródło:
Kosmos; 2017, 66, 2; 193-206
0023-4249
Pojawia się w:
Kosmos
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
mRNA expressions MMP-2 and MMP-9 as potential molecular markers for distinguishing the boundary between tumour and normal tissue in basal cell carcinoma
mRNA dla MMP-2 i MMP-9 jako potencjalne markery molekularne pozwalające na rozróżnienie granicy między guzem a tkanką prawidłową w przypadku raka BCC
Autorzy:
Goździalska, Anna
Pasek, Małgorzata
Jochymek, Małgorzata
Goździalska, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2129676.pdf
Data publikacji:
2022
Wydawca:
Krakowska Akademia im. Andrzeja Frycza Modrzewskiego
Tematy:
BCC
MMPs
molecular markers
collagen type I and IV
markery molekularne
kolagen typu I i IV
MMP
Opis:
Introduction: Non-melanoma skin cancers (NMSC) are the most common malignant neoplasms, the number of which continues to increase. BCC is characterized by slow growth, its main clinical feature being that it infiltrates adjacent tissues and destroys adjacent structures. Material and methods: The expression of mRNA transcripts for collagen types I, IV and MMP-2 and MMP-9 were compared in skin biopsies from patients with BCC of the skin and in the biopsies of healthy skin from the tumour margin. The study involved seventy patients diagnosed with BCC. Results: The differences between mRNA expressions for MMP-2 and MMP-9, type I collagen and type IV collagen were investigated in nodular and infiltrative BCC both in tumour tissue samples and normal tissue samples taken from the tumour margins of the same patients. Conclusions: Significantly higher levels of mRNA expressions for type I collagen, MMP-2 and MMP-9, as well as consistently lower levels of mRNA expression for type IV collagen in tumour tissue compared to tumour margin tissue obtained from the same patients, were identified in both types of BCC. These differences indicate a different role for collagen I and collagen IV in the pathomechanism of BCC.
Wprowadzenie: Nieczerniakowe nowotwory skóry (NMSC) są najczęstszymi nowotworami złośliwymi, których liczba stale rośnie. BCC charakteryzuje się powolnym wzrostem, a główną cechą kliniczną tego guza jest naciekanie okolicznych tkanek i niszczenie sąsiadujących struktur. Materiał i metody: Ekspresję transkryptów mRNA dla kolagenu typu I i IV oraz MMP-2 i MMP-9 porównano w biopsjach skóry od pacjentów z BCC skóry oraz w biopsjach zdrowej skóry z marginesu guza. W badaniu wzięło udział 70 pacjentów, u których zdiagnozowano BCC. Wyniki: Stwierdzono różnice między ekspresją mRNA dla MMP-2 i MMP-9, kolagenu typu I i IV w BCC guzkowym i naciekowym w próbkach tkanek nowotworowych i tkanek prawidłowych pobranych z marginesów guza tych samych pacjentów. Wnioski: Istotnie wyższe poziomy ekspresji mRNA dla kolagenu typu I, MMP-2 i MMP-9, a także zawsze niższe poziomy ekspresji mRNA dla kolagenu typu IV w tkance nowotworowej w porównaniu z tkanką marginesu guza uzyskaną od tych samych pacjentów wykazano w obu typach BCC. Postrzegane różnice wskazują na inną rolę kolagenu I i kolagenu IV w patomechanizmie BCC.
Źródło:
Państwo i Społeczeństwo; 2022, 1; 35-53
1643-8299
2451-0858
Pojawia się w:
Państwo i Społeczeństwo
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Charakterystyka wybranych markerów molekularnych
Characteristic of selected molecular markers
Autorzy:
Bolc, Paulina
Powiązania:
https://bibliotekanauki.pl/articles/2199379.pdf
Data publikacji:
2020-10-22
Wydawca:
Instytut Hodowli i Aklimatyzacji Roślin
Tematy:
markery molekularne
RFLP
AFLP
RAPD
SSR
ISSR
SRAP
SNP
PCR
polimorfizm
różnorodność genetyczna
molecular markers
polymorphism
genetic diversity
Opis:
Postęp, jaki nastąpił w biologii molekularnej poprzez wprowadzenie markerów molekularnych nowej generacji, w ciągu ostatnich 20 lat umożliwił znaczny rozwój wielu dziedzin badań. Możliwe stało się uzyskanie dokładniejszych informacji genetycznych pozwalających na lepsze zrozumienie zasobów genetycznych organizmów. Markerem molekularnym może być każda sekwencja nukleotydowa (wybrany fragment DNA), rozproszony w całym genomie, której zmienność między osobnikami lub grupami taksonomicznymi umożliwia precyzyjną identyfikację osobnika/taksonu. Kompilacja właściwości enzymów restrykcyjnych jak również reakcji łańcuchowej polimerazy (PCR) w technikach generujących markery molekularne pozwoliła na efektywne wykorzystanie ich w taksonomicznych, ewolucyjnych i ekologicznych badaniach roślin.
Over the last 20 years, the progress in molecular biology through the introduction of new generation molecular markers has allowed many areas to move forward. It has now become possible to obtain more accurate genetic information to better understand the genetic resources of organisms. A molecular marker can be any nucleotide sequence (selected DNA fragment) scattered throughout the genome, whose variability between individuals or taxonomic groups allows precise identification of the individual/taxon. The effectiveness of restriction digestion and polymerase chain reaction based on molecular markers has already proved their usefulness in taxonomic, evolutionary and ecological plant research.
Źródło:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin; 2020, 290; 27-32
0373-7837
2657-8913
Pojawia się w:
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Zmienność genetyczna cisa w wybranych rezerwatach Polski
Genetic variability of yew in the selected Polish nature reserves
Autorzy:
Nawrocka-Grześkowiak, U.
Skuza, L.
Szucko, I.
Powiązania:
https://bibliotekanauki.pl/articles/989813.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Leśne
Tematy:
lesnictwo
drzewa lesne
cis pospolity
Taxus baccata
zmiennosc genetyczna
rezerwaty przyrody
populacje roslin
zroznicowanie genetyczne
markery genetyczne
markery RAPD
genetic diversity
molecular markers
taxus baccata
nature reserv
Opis:
Genetic variability of yew (Taxus baccata L.) trees in seven nature reserves (Jasień, Czarne Człuchowskie, Choczewo, Wierzchlas, Wirty, Rokita and Mogilno/Stary Sącz) in Poland was investigated by RAPD methods. The results showed high genetic differences between populations ranging from 22.7% among the population of Stary Sącz and hedge (+) to 86.1% between Wierzchlas and Rokita (+). Based on the results of RAPD analyses, the studied populations were divided into three groups of similarity. The first include Jasień and Choczewo the populations, the second Rokita (+), hedge (–) and Stary Sącz, and the third remaining populations. High level of genetic diversity was showed among the studied populations, is essential to the adaptability of the population of yew. This theoretically increases the chances of survival species thanks to the possibility of passing to preferred combinations of genes to the future generations.
Źródło:
Sylwan; 2015, 159, 06; 491-497
0039-7660
Pojawia się w:
Sylwan
Dostawca treści:
Biblioteka Nauki
Artykuł
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