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Tytuł:
Lansoprazole is an uncompetitive inhibitor of tissue-nonspecific alkaline phosphatase
Autorzy:
Delomenède, Mélanie
Buchet, René
Mebarek, Saïda
Powiązania:
https://bibliotekanauki.pl/articles/1040588.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
phosphocholine
PHOSPHO1
inhibition
lansoprazole
alkaline phosphatase
osteoarthritis
Opis:
Lansoprazole, a known H/K+-ATPase inhibitor, is currently used as a therapeutical option for the initial treatment of gastroesophageal reflux disease. Recently, lansoprazole has been found to be an inhibitor of cytosolic PHOSPHO1 (a phosphatase which hydrolyses phosphocholine and phosphoethanolamine), providing a possible therapeutical target to cure pathological mineralization. Since PHOSPHO1 is present inside matrix vesicles, we tested the effect of lansoprazole on matrix vesicles containing several key enzymes for the mineralization process including tissue-nonspecific alkaline phosphatase. We found that lansoprazole can inhibit in an uncompetitive manner tissue-nonspecific alkaline phosphatase. A Ki value of 1.74 ± 0.12 mM has been determined for the inhibition of tissue-nonspecific alkaline phosphatase by lansoprazole. Lansoprazole, currently used for treating gastroesophageal disease, by inhibiting PHOSPHO1 and tissue-nonspecific alkaline phosphatase could prevent hydroxyapatite-deposition disease and could serve as an adjunct treatment for osteoarthritis.
Źródło:
Acta Biochimica Polonica; 2009, 56, 2; 301-306
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Plasma membrane homing of tissue nonspecific alkaline phosphatase under the influence of 3-hydrogenkwadaphnin, an antiproliferative agent from Dendrostellera lessertii
Autorzy:
Sadeghirizi, Akram
Yazdanparast, Razieh
Powiązania:
https://bibliotekanauki.pl/articles/1041081.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
alkaline phosphatase
anti-proliferative
Dendrostellera lessertii
Opis:
Several mammalian enzymes are anchored to the outer surface of the plasma membrane by a covalently attached glycosylphosphatidylinositol (GPI) structure. These include acetylcholinesterase, alkaline phosphatase (AP) and 5'-nucleotidase among other enzymes. Recently, it has been reported that these membrane enzymes can be released into the serum by the GPI-dependent phospholipase D under various medical disturbances such as cancer and/or by chemical and physical manipulation of the biological systems. Treatment of MCF-7 cells with two consecutive effective concentrations of 3-hydrogenkwadaphnin (3-HK, 3 nM) for 48 h enhanced membrane AP activity by almost 330% along with a 40% reduction in the AP activity of the cell culture medium. In addition, our data indicate that 3-HK is capable of inducing mainly the tissue-nonspecific alkaline phosphatase (TNAP) isoenzyme, along with enhancing its thermostability. These findings, besides establishing a correlation between the antiproliferative activity of 3-HK and the extent of plasma membrane AP activity, might assist in the development of new diagnostic tools for following cancer medical treatments.
Źródło:
Acta Biochimica Polonica; 2007, 54, 2; 323-329
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The roles of annexins and alkaline phosphatase in mineralization process.
Autorzy:
Balcerzak, Marcin
Hamade, Eva
Zhang, Le
Pikula, Slawomir
Azzar, Gérard
Radisson, Jacqueline
Bandorowicz-Pikula, Joanna
Buchet, Rene
Powiązania:
https://bibliotekanauki.pl/articles/1043372.pdf
Data publikacji:
2003
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mineralization
hydroxyapatite
alkaline phosphatase
annexin
matrix vesicle
Opis:
In this review the roles of specific proteins during the first step of mineralization and nucleation are discussed. Mineralization is initiated inside the extracellular organelles-matrix vesicles (MVs). MVs, containing relatively high concentrations of Ca2+ and inorganic phosphate (Pgi), create an optimal environment to induce the formation of hydroxyapatite (HA). Special attention is given to two families of proteins present in MVs, annexins (AnxAs) and tissue-nonspecific alkaline phosphatases (TNAPs). Both families participate in the formation of HA crystals. AnxAs are Ca2+- and lipid-binding proteins, which are involved in Ca2+ homeostasis in bone cells and in extracellular MVs. AnxAs form calcium ion channels within the membrane of MVs. Although the mechanisms of ion channel formation by AnxAs are not well understood, evidence is provided that acidic pH or GTP contribute to this process. Furthermore, low molecular mass ligands, as vitamin A derivatives, can modulate the activity of MVs by interacting with AnxAs and affecting their expression. AnxAs and other anionic proteins are also involved in the crystal nucleation. The second family of proteins, TNAPs, is associated with Pi homeostasis, and can hydrolyse a variety of phosphate compounds. ATP is released in the extracellular matrix, where it can be hydrolyzed by TNAPs, ATP hydrolases and nucleoside triphosphate (NTP) pyrophosphohydrolases. However, TNAP is probably not responsible for ATP-dependent Ca2+/phosphate complex formation. It can hydrolyse pyrophosphate (PPi), a known inhibitor of HA formation and a byproduct of NTP pyrophosphohydrolases. In this respect, antagonistic activities of TNAPs and NTP pyrophosphohydrolases can regulate the mineralization process.
Źródło:
Acta Biochimica Polonica; 2003, 50, 4; 1019-1038
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Spatiotemporal variation of alkaline phosphatase activity in coastal waters off Trivandrum
Autorzy:
Shivaramu, M.S.
Randive, A.K.
Kumari, R.
Gauns, M.
Ponnapakkam, L.A.
Powiązania:
https://bibliotekanauki.pl/articles/48698.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Instytut Oceanologii PAN
Tematy:
phosphatase
alkaline phosphatase
chlorophyll a
phytoplankton
bacteria
inorganic nutrient
organic carbon
aquatic environment
spatial variation
temporal variation
Źródło:
Oceanologia; 2019, 61, 1
0078-3234
Pojawia się w:
Oceanologia
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insertion of GPI-anchored alkaline phosphatase into supported membranes: a combined AFM and fluorescence microscopy study.
Autorzy:
Rieu, Jean-Paul
Ronzon, Frédéric
Place, Christophe
Dekkiche, Fairouz
Cross, Benjamin
Roux, Bernard
Powiązania:
https://bibliotekanauki.pl/articles/1043341.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fluorescent beads
atomic force microscopy (AFM)
GPI anchors
alkaline phosphatase
supported lipid membranes
Opis:
A new method based on combined atomic force microscopy (AFM) and fluorescence microscopy observations, is proposed to visualize the insertion of glycosylphosphatidyl inositol (GPI) anchored alkaline phosphatase from buffer solutions into supported phospholipid bilayers. The technique involves the use of 27 nm diameter fluorescent latex beads covalently coupled to the amine groups of proteins. Fluorescence microscopy allows the estimation of the relative protein coverage into the membrane and also introduces a height amplification for the detection of protein/bead complexes with the AFM. The coupling of the beads with the amine groups is not specific; this new and simple approach opens up new ways to investigate proteins into supported membrane systems.
Źródło:
Acta Biochimica Polonica; 2004, 51, 1; 189-197
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Structural research of thermosensitive chitosan-collagen gels containing alp
Autorzy:
Skwarczyńska, Agata
Biniaś, Dorota
Modrzejewska, Zofia
Powiązania:
https://bibliotekanauki.pl/articles/1034675.pdf
Data publikacji:
2016
Wydawca:
Sieć Badawcza Łukasiewicz - Polskie Towarzystwo Chitynowe
Tematy:
alkaline phosphatase
chitosan
collagen
thermosensitive hydrogels
Opis:
Introducing collagen, which is basic ingredient of bone tissue, into the structure of chitosan gels which are formed at the physiological body temperature, is aimed at creating the so-called biomimetic structures, i.e. close in their composition to the natural composition of bone tissue. Within the research the influence of collagen on structural properties of thermosensitive chitosan gels and the influence of ALP on structural properties of chitosan and chitosan-collagen gels was determined.
Źródło:
Progress on Chemistry and Application of Chitin and its Derivatives; 2016, 21; 176-186
1896-5644
Pojawia się w:
Progress on Chemistry and Application of Chitin and its Derivatives
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Difficulties in diagnosis and treatment of Paget’s disease
Autorzy:
Kawalec, Aleksandra
Zdrojowy-Wełna, Aleksandra
Bednarek-Tupikowska, Grażyna
Powiązania:
https://bibliotekanauki.pl/articles/551935.pdf
Data publikacji:
2016
Wydawca:
Stowarzyszenie Przyjaciół Medycyny Rodzinnej i Lekarzy Rodzinnych
Tematy:
Paget’s disease
alkaline phosphatase
bisphosphonates.
Źródło:
Family Medicine & Primary Care Review; 2016, 4; 501-505
1734-3402
Pojawia się w:
Family Medicine & Primary Care Review
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Remediation of soil contaminated with cadmium
Autorzy:
Zaborowska, M.
Kucharski, J.
Wyszkowska, J.
Powiązania:
https://bibliotekanauki.pl/articles/958240.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
soil contamination
cadmium
alkaline phosphatase
acid phosphatase
soil microorganism
soil remediation
Opis:
The search for the best solutions to restore soil balance is essential for attaining a stable and sustainable agricultural development worldwide. This research, which makes a contribution to these investigations, focuses on four substances (two innovative ones: basalt flour, brown algal extract, and two classic soil improvers: finely ground barley straw and compost) which can potentially alleviate the inhibitory effect of Cd2+ on the soil environment. The following were analyzed: the activity of acid phosphatase and alkaline phosphatase, counts of Pseudomonas sp., cellulolytic bacteria, copiotrophic bacteria and copiotrophic spore-forming bacteria, and the yield of spring barley. Cadmium (Cd2+) was applied as CdCl2 · 2.5H2O in the following doses: 0, 4, 40, 80, 120, 160, and 200 mg Cd2+ kg-1 of soil. For a more complete assessment of the soil, its biochemical properties and the counts of microorganisms were scrutinized with the following indices: RS – soil resistance, R:S – rhizosphere effect and EF – fertilization effect of the contamination alleviating substances. It was found that alkaline phosphatase is more sensitive to cadmium contamination of the soil than acid phosphatase. Cadmium did not exert any inhibitory effect on the number of microorganisms present or the yield of spring barley. Cellulolytic bacteria were the least sensitive to stress associated with the accumulation of high cadmium doses in the soil, whereas copiotrophic bacteria were the most sensitive microorganisms to the above stressor. The ability of cadmium-polluted soil to restore homeostasis depended on the type of a soil improver and the level of soil contamination. Negative consequences of cadmium pollution were effectively mitigated by straw, but less so by brown algal extract and basalt flour.
Źródło:
Journal of Elementology; 2015, 20, 3
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isotope effects on enzymatic and nonenzymatic reactions of phosphorothioates
Autorzy:
Catrina, I.
Czyryca, P.
Hengge, A.
Powiązania:
https://bibliotekanauki.pl/articles/146931.pdf
Data publikacji:
2002
Wydawca:
Instytut Chemii i Techniki Jądrowej
Tematy:
alkaline phosphatase
isotope effect
phosphoryl transfer
phosphorothioate
thio effect
Opis:
Kinetic isotope effects have been measured for the aqueous hydrolysis reactions of p-nitrophenyl phosphorothioate (pNPPT) and the diester ethyl p-nitrophenyl phosphorothioate, and for the alkaline phosphatase-catalyzed reaction with pNPPT. The results show that the transition states of the uncatalyzed reactions of the phosphorothioate mono- and diesters are very similar to those of the corresponding phosphate ester reactions. The secondary 18O nonbridge isotope effects in reactions of phosphate esters become more normal as the mechanism changes from dissociative, metaphosphate-like to associative, phosphorane-like. The opposite trend occurs in phosphorothioate esters, due to differences in the relative contributions of bond-order changes and bending modes to this isotope effect. The KIEs for the alkaline phosphatase-catalyzed reaction of pNPPT are most consistent with a tight, triester-like transition state, probably a result of perturbations resulting from the larger size of sulfur that lead to a nucleophile attack angle that is unfavorable for an in-line process with a loose transition state.
Źródło:
Nukleonika; 2002, 47,suppl.1; 17-23
0029-5922
1508-5791
Pojawia się w:
Nukleonika
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ poli(ε-kaprolaktonu) na ekspresję i aktywność fosfatazy alkalicznej w ludzkich komórkach osteogennych
The effect of poly(ε-caprolactone) on the expression and activity of alkaline phosphatase in human osteogenic cells
Autorzy:
Leszczyńska, J.
Wójtowicz, J.
Olkowski, R.
Komasa, J.
Ulański, P.
Lewandowska-Szumieł, M.
Powiązania:
https://bibliotekanauki.pl/articles/286189.pdf
Data publikacji:
2011
Wydawca:
Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
Tematy:
poli(ε-kaprolakton)
osteoblasty
fosfataza zasadowa
różnicowanie
poly(ε-caprolactone)
osteoblasts
alkaline phosphatase
differentiation
Opis:
Poli(ε-kaprolakton) jest materiałem wykorzystywanym jako rusztowanie dla komórek w inżynierii tkankowej kości. Na podstawie danych z literatury oraz naszych własnych badań nad reakcją komórek osteogennych na bezpośredni kontakt z poli(ε-kaprolaktonem) można przypuszczać, iż materiał ten może wpływać na poziom markerów różnicowania komórek w kierunku osteoblastów. Celem niniejszej pracy było zbadanie wpływu poli(ε-kaprolaktonu) na ekspresję oraz aktywność wczesnego markera procesu różnicowania komórek osteogennych, jakim jest fosfataza zasadowa. Przy użyciu reakcji łańcuchowej polimerazy DNA z analizą ilości produktu w czasie rzeczywistym (real-time PCR) analizowano ekspresję genu fosfatazy zasadowej natomiast aktywność enzymu oznaczono kolorymetrycznym testem firmy Sigma. Otrzymane wyniki wskazują, iż kontakt ludzkich osteoblastów z powierzchnią poli(ε-kaprolaktonu) powoduje podwyższoną ekspresję genu fosfatazy zasadowej oraz podwyższoną aktywność tego enzymu. Fosfataza zasadowa nie jest specyficznym markerem osteoblastów, jednakże jej podwyższony poziom towarzyszy wczesnym etapom różnicowania w kierunku fenotypu komórek osteogennych. Uzyskane wyniki uzasadniają podjęcie dalszych badań nad możliwym wpływem poli(ε-kaprolaktonu) na różnicowanie osteoblastów.
Poly(ε-caprolactone) is a material used as a scaffold for cells in bone tissue engineering. On the basis of data from literature as well as own research it was concluded that this material can influence the levels of markers of cell differentiation towards osteoblasts. The aim of this paper was to investigate the effect of poly(ε-caprolactone) on the expression and the activity of the early marker of the cell osteogenic differentiation process – alkaline phosphatase (ALP). Using the quantitative real time polymerase chain reaction (real-time PCR) gene expression of the alkaline phosphatase was analyzed; however, the activity of the enzyme was determined with colorimetric assay from the Sigma company. The obtained results indicated that the contact of human osteoblasts with the surface of poly(ε-caprolactone) causes an increased gene expression of alkaline phosphatase and an increased activity of this enzyme. Although a high level of ALP does not prove the PCL influence on the osteogenic differentiation of cells into mature osteoblasts, because this enzyme is a non-specific marker of the differentiation process. The obtained results justify undertaking further studies on the possible impact of poly(ε-caprolactone) on osteoblast differentiation.
Źródło:
Engineering of Biomaterials; 2011, 14, 103; 13-16
1429-7248
Pojawia się w:
Engineering of Biomaterials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of different zinc doses and organic fertilization on soil’s enzymatic activity
Autorzy:
Kuziemska, B.
Wysokinski, A.
Klej, P.
Powiązania:
https://bibliotekanauki.pl/articles/1192090.pdf
Data publikacji:
2020
Wydawca:
Uniwersytet Warmińsko-Mazurski w Olsztynie / Polskie Towarzystwo Magnezologiczne im. Prof. Juliana Aleksandrowicza
Tematy:
zinc
organic materials
urease
dehydrogenases
acid phosphatase
alkaline phosphatase
Opis:
The enzymatic activity of soil was studied in a three-year pot experiment, where different amounts of zinc were introduced: 0, 200, 400, and 600 mg of Zn kg-1 of soil, as well as organic materials (control object, bovine manure, laying hens’ litter, spent mushroom substrate), used separately with the soil application dose of 2 g Corg kg-1 soil. Zinc and organic materials were used once, only in the first year of research, before sowing the test plant. In each year of the experiment, the test plant was orchard grass (Dactylis glomerata L.), harvested after 4 cuts. The activity of urease, dehydrogenases, as well as acid and alkaline phosphatase were determined in the soil collected after the last grass cutting, in each year of the study. Application of zinc to soil, regardless of its dose, resulted in a decrease in the activity of urease, dehydrogenase and alkaline phosphatase, although the significance of differences in relation to the control object was demonstrated only at higher doses, such as 400 and 600 mg Zn kg-1 soil. The study showed no negative effect of zinc on acid phosphatase activity. Organic fertilizers generally resulted in an increase in the enzymatic activity of the analyzed soil, and simultaneously limited the negative effects of higher zinc doses (400 and 600 mg Zn kg-1 of soil) on urease and dehydrogenase activity. During the consecutive years, urease and alkaline phosphatase activity decreased, while acid phosphatase activity increased. Dehydrogenase activity did not change significantly in the subsequent years of the study.
Źródło:
Journal of Elementology; 2020, 25, 3; 1089-1099
1644-2296
Pojawia się w:
Journal of Elementology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic activity in soil treated with exogenous organic matter
Autorzy:
Siebielec, Sylwia
Siebielec, Grzegorz
Ukalska-Jaruga, Aleksandra
Urbaniak, Magdalena
Powiązania:
https://bibliotekanauki.pl/articles/2148185.pdf
Data publikacji:
2021-12-30
Wydawca:
Instytut Uprawy Nawożenia i Gleboznawstwa – Państwowy Instytut Badawczy
Tematy:
dehydrogenases
acidic phosphatase
alkaline phosphatase
manure
sewage sludge
bottom sediment
Opis:
The content of organic matter affects the quality and productivity of agricultural soils. Due to the shortage of manure as the basic source of organic matter for agricultural soils, al- ternative and commonly available exogenous sources of organic matter, such as sewage sludge and bottom sediments, are taken into account. The aim of the study was to evaluate the effect of the addition of various types of manure, municipal sewage sludge and bottom sediments on the enzymatic activity of soils (dehydrogenases, acidic and alkaline phosphatases). Manure and sludge were applied to the soil in two doses, corresponding to 20 and 40 tons of dry matter of material per ha. The control was soil fertilized with ammonium nitrate in the amount corresponding to 170 kg N per ha. The experiment included 2 different variants of the same fertilization combinations: without plants and with a test plant (Sinapis alba L.). The greenhouse experiment showed the stimulating effect of one of the municipal sewage sludge and poultry manure. On the other hand, the introduction of bottom sediments to the soil limited its enzymatic activity.
Źródło:
Polish Journal of Agronomy; 2021, 47; 87-94
2081-2787
Pojawia się w:
Polish Journal of Agronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Impact of chlortetracycline and sulfapyridine antibiotics on soil enzyme activities
Autorzy:
Molaei, Ali
Lakzian, Amir
Datta, Rahul
Haghnia, Gholamhosain
Astaraei, Alireza
Rasouli-Sadaghiani, MirHassan
T. Ceccherini, Maria
Powiązania:
https://bibliotekanauki.pl/articles/972897.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
alkaline phosphatase
chlortetracycline
dehydrogenase
sulfapyridine
urease
Opis:
Pharmaceutical antibiotics are frequently used in the livestock and poultry industries to control infectious diseases. Due to the lack of proper guidance for use, the majority of administrated antibiotics and their metabolites are excreted to the soil environment through urine and feces. In the present study, we used chlortetracycline and sulfapyridine antibiotics to screen out their effects on dehydrogenase, alkaline phosphatase and urease activity. Factorial experiments were conducted with different concentrations of antibiotic (0, 10, 25 and 100 mg kg-1 of soil) mixed with soil samples, and the enzyme activity was measured at intervals of 1, 4 and 21 days. The results show that the chlortetracycline and sulfapyridine antibiotics negatively affect the dehydrogenase activity, but the effect of sulfapyridine decreases with time of incubation. Indeed, sulfapyridine antibiotic significantly affect the alkaline phosphatase activity for the entire three-time interval, while chlortetracycline seems to inhibit its activity within 1 and 4 days of incubation. The effects of chlortetracycline and sulfapyridine antibiotics on urease activity appear similar, as they both significantly affect the urease activity on day 1 of incubation. The present study concludes that chlortetracycline and sulfapyridine antibiotics have harmful effects on soil microbes, with the extent of effects varying with the duration of incubation and the type of antibiotics used.
Źródło:
International Agrophysics; 2017, 31, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw odczynu [pH] na aktywnosc alkalicznych fosfataz w wodach powierzchniowych mierzona metoda spektrofotometryczna
Autorzy:
Siwek, H
Lewandowska, L.
Powiązania:
https://bibliotekanauki.pl/articles/799598.pdf
Data publikacji:
2006
Wydawca:
Szkoła Główna Gospodarstwa Wiejskiego w Warszawie. Wydawnictwo Szkoły Głównej Gospodarstwa Wiejskiego w Warszawie
Tematy:
spektrofotometria
aktywnosc enzymatyczna
fosfataza zasadowa
odczyn wody
wody powierzchniowe
spectrophotometry
enzyme activity
alkaline phosphatase
water reaction
pH
surface water
Opis:
W pracy badano zmiany aktywności alkalicznych fosfataz w próbach wód naturalnych w obecności buforów o różnym odczynie pH (7,5 - 9,0). Wraz ze wzrostem odczynu pH wzrastała szybkość maksymalna (vmax) oraz stała Michaelisa-Menten (KM) enzymatycznej reakcji hydrolizy p-nitrofenylofosforanu disodu. Optymalny odczyn pH przy oznaczaniu aktywności alkalicznej fosfatazy w wodach naturalnych wyniósł 8,5.
The changes of alkaline phosphatase activity (APA) in water samples with the buffers at different pH (7.5 - 9.0) were studied. The Michaelis-Menten constant (KM) and maximum rate (vmax) of disodium p-nitrophenylphosphate hydrolysis increased with an increase of pH. The optimum reaction for phosphohydrolitic activity was observed at pH 8.5.
Źródło:
Zeszyty Problemowe Postępów Nauk Rolniczych; 2006, 515; 365-370
0084-5477
Pojawia się w:
Zeszyty Problemowe Postępów Nauk Rolniczych
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ selenu i fluoru na aktywność fosfataz w glebie lekkiej
Effect of selenium and fluoride on phoshatase activities in sandy soil
Autorzy:
Onyszko, M.
Telesiński, A.
Powiązania:
https://bibliotekanauki.pl/articles/401499.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
fosfatazy
fluor
gleba
selen
wskaźnik oporności
acid phosphatase
alkaline phosphatase
fluoride
soil
selenium
resistance index
Opis:
Celem podjętych badań było określenie oddziaływania związków fluoru oraz selenu (na dwóch stopniach utlenienia: IV i VI), wprowadzonych osobno oraz w mieszaninie na aktywność fosfatazową gleby lekkiej. Doświadczenie przeprowadzono w warunkach laboratoryjnych na piasku gliniastym o zawartości węgla organicznego 8,7 g/kg, do którego wprowadzono w różnych kombinacjach fluor (10 mmol/kg) oraz selen (0,05 mmol/kg). W odstępach tygodniowych przez okres 28 dni oznaczano spektrofotometrycznie aktywność fosfatazy alkalicznej i fosfatazy kwaśnej. Na podstawie otrzymanych wyników obliczono wskaźniki oporności enzymów (RS). Wykazano że wprowadzenie do gleby fluoru i selenu spowodowało istotne statystycznie obniżenie aktywności oznaczanych enzymów. Aktywność fosfatazy alkalicznej charakteryzowała się większą wrażliwością na obecność fluoru i selenu w glebie niż fosfataza kwaśna. Dla fosfatazy alkalicznej największe obniżenie RS wystąpiło po aplikacji fluoru wraz z selenem na stopniu utlenienia IV, a dla fosfatazy kwaśnej – fluoru wraz z selenem na stopniu utlenienia VI.
The aim of the study was to determine the impact of fluoride and selenium (in two oxidation states IV and VI), introduced separately and in a mixture on soil phosphatase activity soil. The experiment was carried out in laboratory conditions on loamy sand samples with organic carbon content 8.7 g/kg. Various combinations of fluoride (10 mmol kg) and selenium (0.05 mmol/kg) were added into soil samples. Over a period of 28 days, at weekly intervals. Alkaline phosphatase and acid phosphatase activities were determined by spectrophotometry. Based on the obtained results, the resistance index (RS) for enzymes was calculated. It was found that the addition of fluoride and selenium into the soil resulted in a statistically significant decrease of assayedenzyme activities. Alkaline phosphatase activity was more sensitive to the presence of fluoride and selenium in the soil than acid phosphatase. The values of RS were lowest for alkaline phosphatase in soil treated with fluoride and selenium in the oxidation state IV, and for acid phosphatase in soil treated with fluoride and selenium in the oxidation state VI.
Źródło:
Inżynieria Ekologiczna; 2015, 43; 109-114
2081-139X
2392-0629
Pojawia się w:
Inżynieria Ekologiczna
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 29

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