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    Wyświetlanie 1-5 z 5
    Tytuł:
    Molecular epidemiology and phylogenetic analysis of Tams I gene of Theileria annulata in Khyber Pakhtunkhwa, Pakistan
    Autorzy:
    Shah, S.S.A.
    Saddique, U.
    Khan, M.A.
    Khan, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647390.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    crossbred cattle
    Pakistan
    PCR
    risk factors
    sequencing
    theileriosis
    Opis:
    Theileriosis is a hemoparasitic disease that affects a wide range of different animal species and is caused by various species of Theileria. This study aimed to determine the molecular epidemiology of Theileria annulata through microscopy and PCR, in crossbred cattle in some districts of Khyber Pakhtunkhwa, Pakistan. For this study, a total of 384 blood samples were collected from cattle in the Peshawar (n=120), Charsadda (n=94), Nowshera (n=84), and Swabi (n=86) districts. Microscopy and PCR were used to determine the overall prevalence of theileriosis, which was found to be 15.8 and 22.6%, respectively. Theileria annulata was detected in blood samples through PCR in the study area, and the target gene i.e., Tams 1, of positive samples was sequenced. The sequences in the current study revealed high sequence homology (ranging from 96 to 100%) with Tams 1 sequences of neighboring countries present in the NCBI database. Season, breed, age, and sex were found to be important risk factors among the several risk factors examined, whereas, among different clinical manifestations, lymphadenopathy showed a strong association with theileriosis. According to Cohen’s kappa and ROC analysis, microscopy was proven to be a fair diagnostic test for detecting theileriosis in cattle, and may be used in combination with molecular techniques for screening a large number of animals.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 4; 625-629
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Biodiversity in morphological and physico-chemical characteristics of wild raspberry (Rubus idaeus L.) germplasm collected from temperate region of Azad Jammu and Kashmir (Pakistan)
    Różnorodność biologiczna w morfologicznych i fizyko-chemicznych cechach materiału genetycznego maliny właściwej (Rubus idaeus L.) zbieranej w regionie umiarkowanym Azad Jammu i Kaszmiru (Pakistan)
    Autorzy:
    Ahmed, M.
    Anjum, M.A.
    Khaqan, K.
    Hussain, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/11542966.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    biodiversity
    morphological characteristics
    physicochemical characteristics
    wild plant
    raspberry
    Rubus idaeus
    germplasm
    temperate region
    Azad Jammu and Kashmir
    Pakistan
    Opis:
    Wild raspberry (Rubus idaeus L.) is abundantly grown and widely distributed in temperate regions of lower Himalayan range which represents rich biological diversity for raspberry. Thirty accessions were explored from three different locations of Azad Jammu and Kashmir (Pakistan), several traits were visualized at sampling locations and some characteristics were evaluated and quantified after harvest of leaf and fruit samples. The accessions differed greatly in growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, fruit shape and color. Concerning quantitative characteristics, significant differences were found in plant height, leaf length and width, number of braches per plant, duration of flowering, fruit setting percentage, days to maturity, fruit length, width and weight among the samples collected from different locations. Ripe fruits were evaluated for their chemical composition i.e. moisture, carbohydrates, protein, fat, fiber, ash, TSS, acidity, pH and mineral (K, Ca, Na, Fe, Cu, Zn, Mn, Pb, and Cr) contents. The location also had significant effect on chemical composition of ripe fruits.
    Malina właściwa (Rubus idaeus L.) jest często hodowana i szeroko rozprowadzana w umiarkowanych rejonach niższego pasma Himalajów, które reprezentuje bogatą różnorodność biologiczną malin. Zbadano trzydzieści próbek z trzech różnych miejsc Azad Jammu i Kaszmiru (Pakistan), dokonano wizualizacji kilkunastu cech w miejscach pobierania próbek oraz oceniono i ilościowo określono kilka cech po zebraniu próbek liści i owoców. Próbki znacznie różniły się wzrostem, czasem kwitnienia, intensywnością zakwitania, zawiązywaniem owoców, czasem dojrzewania, plonowaniem oraz kształtem i kolorem owoców. Jeśli chodzi o cechy ilościowe, to stwierdzono istotne różnice w wysokości roślin, długości i szerokości liści, liczbie gałązek na roślinie, długości kwitnienia, procencie zawiązywanych owoców, liczbie dni do dojrzałości, długości owoców, szerokości i wadze pomiędzy próbkami zebranymi z różnych stanowisk. Dojrzałe owoce oceniono pod względem składu chemicznego, tzn. wilgotności, zawartości węglowodanów, białka, tłuszczu, błonnika, popiołu, TSS, kwasowości, pH oraz zawartości minerałów (K, Ca, Na, Fe, Cu, Zn, Mn, Pb oraz Cr). Stanowisko także miało istotny wpływ na skład chemiczny dojrzałych owoców.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2014, 13, 4; 117-134
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Genetic diversity of Brassica rapa germplasm of Khyber Pakhtunkhwa Pakistan revealed by molecular markers
    Autorzy:
    Ali, N.
    Ali, S.
    Khan, N.U.
    Jan, S.A.
    Rabbani, M.A.
    Hussain, I.
    Powiązania:
    https://bibliotekanauki.pl/articles/12690092.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pakistan
    plant breeding
    Brassica rapa
    germplasm
    genetic diversity
    plant genotype
    molecular marker
    SSR marker
    Opis:
    A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 6; 57-65
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Seed priming and foliar application of plant growth regulators affect the growth and yield of okra under calcareous soils
    Autorzy:
    Tahir, M.T.
    Anjum, M.A.
    Saqib, M.
    Khalid, M.F.
    Hussain, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/12664203.pdf
    Data publikacji:
    2019
    Wydawca:
    Uniwersytet Przyrodniczy w Lublinie. Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie
    Tematy:
    Pakistan
    plant cultivation
    okra
    Abelmoschus esculentus
    seed priming
    plant growth
    growth regulator
    growth regulator effect
    foliar application
    naphthaleneacetic acid
    gibberellin A3
    Opis:
    A field study was conducted to evaluate the effect of different plant growth regulators (PGRs) applied through seed priming and foliar spray on growth and yield of three okra cultivars grown under calcareous soils. The cultivars of Punjab Selection and Sabzpari produced significantly higher number of branches and leaves per plant, pod length and diameter and pod yield as compared to cv. Green ferry, while, cv. Punjab selection produced significantly greater number of flowers and pods per plant as compared to other two cultivars. Seed germination (%), plant height, and fruit set (%) were not affected by the cultivars. Among the PGR treatments, seed primed with GA3 resulted in significantly higher germination percentage and greater plant height at flowering. Seed priming and foliar spray with NAA and GA3 were effective in increasing the final plant height, number of branches, number of leaves, number of flowers and number of pods per plant and fruit set (%), fresh weight per pod and pod yield. However, pod diameter, pod moisture content and dry weight per pod were not influenced by the PGR treatments applied. These results suggested that the PGRs have great potential to improve seed germination, enhance growth and increase yield of okra cultivars under calcareous soils.
    Źródło:
    Acta Scientiarum Polonorum. Hortorum Cultus; 2019, 18, 4; 25-33
    1644-0692
    Pojawia się w:
    Acta Scientiarum Polonorum. Hortorum Cultus
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan
    Autorzy:
    Saeed, M.A.
    Waheed, U.
    Ehtisham-ul-Haque, S.
    Khan, A.U.
    Kashif, M.
    Qamar, M.F.
    Ghafoor, A.
    Saqlain, M.
    Asghar, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647457.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    ESBL
    genotypic characterization
    Salmonella enterica
    Escherichia coli
    avifauna
    Pakistan
    Opis:
    Members of Enterobacteriaceae are known to produce extended-spectrum beta-lactamases (ESBL) which hydrolyze the beta-lactam group of antibiotics. The existence of ESBL-producing Salmonella enterica (S. enterica) and Escherichia coli (E. coli) harbored by urban avifauna was investigated in this study. Dropping samples (n= 180) were collected from six different bird species in the district Jhang, Punjab province, Pakistan. Isolation and identification of ESBL isolates were made by using cefotaxime- (4 mg/L) supplemented MacConkey agar and double disc synergy test (DDST). Polymerase chain reaction (PCR) was performed for the detection of four different ESBL genes including bla CTX-M, bla TEM, bla SHV and bla OXA. A total of 42.69% isolates were confirmed as ESBL via DDST including 30.64% S. enterica and 49.54% E. coli. The incidence of ESBL S. enterica and ESBL E. coli was found highest in egret (Ardea alba) and pigeon (Columba livia) as 64.28% and 78.95%, respectively. The bla CTX-M gene was detected in 57.89% and 64.81% of isolates of S. enterica and E. coli, respectively. Among other genes in S. enterica and E. coli, bla TEM (21.05%, 20.4%); bla SHV (15.78%, 9.26%), and bla OXA (5.26%, 5.56%) were detected, respectively. All of the tested isolates were found resistant to at least one of the thirteen antimicrobial agents except meropenem. To the best of our knowledge, this is the first study reporting the incidence and genetic diversity of ESBL bacteria associated with urban avifauna in Pakistan. The urban avifauna can serve as a potential subject of bio-surveillance to monitor the emergence of antimicrobial-resistant bacteria.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 47-55
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-5 z 5

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