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    Wyświetlanie 1-11 z 11
    Tytuł:
    Development and validation of rapid LC-MS with electrospray ionization for the quantification of pramipexole in human plasma
    Autorzy:
    Lavudu, P.
    Rani, A. P.
    Sekharan, C. B.
    Powiązania:
    https://bibliotekanauki.pl/articles/411617.pdf
    Data publikacji:
    2014
    Wydawca:
    Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
    Tematy:
    Pramipexole
    LC-MS
    human plasma
    bioanalytical metod
    Opis:
    A rapid, accurate and precise LC-MS method is described for the quantitative determination of pramipexole in human plasma matrix using ropinirole as internal standard. Pramipexole and ropinirole were extracted from plasma by liquid-liquid extraction technique. The method was validated over the concentration range of 100-2514 pg/mL. The method was found to have acceptable accuracy, precision, linearity and selectivity. The mean extraction recovery from spiked plasma samples was in the range of 79.415-87.00 %. The intra-day accuracy of the assay ranged from 98.924 to 112.236 % and intra-day precision ranged from 3.489 to 6.756 %. Inter-day accuracy and precision results for quality control samples ranged between 100.340 and 107.443% of nominal and precision is observed to be 3.970-5.714 %. The pramipexole was found to be stable after several stability studies. The proposed method yielded a quick, simple and reliable protocol for estimating pramipexole concentrations in human plasma.
    Źródło:
    International Letters of Chemistry, Physics and Astronomy; 2014, 18; 1-15
    2299-3843
    Pojawia się w:
    International Letters of Chemistry, Physics and Astronomy
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Mass Spectrometric Detection of New Betalains in Mammillaria Flowers
    Autorzy:
    Szot, Dominika
    Starzak, Karolina
    Skopińska, Anna
    Wybraniec, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1034229.pdf
    Data publikacji:
    2015
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Cactaceae
    LC-MS
    Mammillaria
    betacyanins
    natural pigments
    Opis:
    Betacyanins are natural, red-violet betalain pigments which can be found in plenty of plants of the Cactaceae family. The Mammillaria is the widest genus of the Cactaceae family of which fruit was examined for betacyanins contents. As a result of the investigation, a new pigment structure, mammillarinin, was identified. Heretofore, no betacyanins of Mammillaria coronata flowers has been extensively studied. In this report, the results of betacyanin analysis by LC-DAD-ESI-MS/MS in the flower extract are presented.
    Źródło:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe; 2015, 10; 189-196
    2082-3827
    2084-977X
    Pojawia się w:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phytochemical composition and antibacterial activity of ethanolic extract from trunk of Rothmannia wittii (Craib) Bremek. (Rubiaceae) newly found in Vietnam
    Autorzy:
    Van, H.T.
    Nguyen, T.T.
    Ton, H.T.T.
    Dinh, K.T.T.
    Le, V.S.
    Pham, T.V.
    Tran, G.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2096309.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Rothmannia wittii
    extract
    phytochemical composition
    antibacterial activity
    LC-MS
    Opis:
    Rothmannia wittii, which is found in Binh Chau-Phuoc Buu Nature Reserve, Bung Rieng ward, Xuyen Moc District, Ba Ria-Vung Tau Province, Vietnam, is a rare species of Rubiaceae family. The present study aimed to determine the phytochemical composition and antimicrobial activity of the ethanolic extract of the R. wittii trunk. A total of 10 compounds were identified by liquid chromatography-mass spectrophotometry (LC-MS), including 1) benzene, 1-(1,5-dimethyl-4- hexenyl)-4-methyl, 2) diphenylsulfone, 3) 6β-hydroxy-10-O-acetylgenipin, 4) cinnamyl-cinnamate, 5) genipin, 6) megastigmatrienone, 7) ethyl p-methoxy-trans-cinnamate, 8) rothalazepane, 9) 1,3,6-trimethylpyrano[2,3-d ]imidazole-2,5(1H,3H)-dione, and 10) shanzhiol. The extract also exhibited an antibacterial activity against six bacterial strains: Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Salmonella enteritidis, as determined via disk diffusion agar method.
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 4; 301-307
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Betaxanthin Profiling in Beta vulgaris Leaves and Gymnocalycium mihanovichii Grafted Cacti: A Comprehensive Study
    Autorzy:
    Spórna-Kucab, Aneta
    Tekieli, Anna
    Knapek, Magdalena
    Zużałek, Marta
    Wybraniec, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/24024625.pdf
    Data publikacji:
    2023-12-05
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    beet
    betalains
    colorants
    grafted cactus
    LC-MS
    secondary metabolites
    Opis:
    This study was focused on the identification and quantification of betaxanthins using high-performance liquid chromatography with a diode array detector coupled to electrospray ionisation mass spectrometry (HPLC-DAD-ESI-MS) in leaves of various cultivars of Beta vulgaris (beet) and Gymnocalycium mihanovichii grafted cacti. In G. mihanovichii grafted cacti, four betaxanthins, namely histidine-Bx, histamine-Bx, serine-Bx, and proline-Bx, were tentatively identified in the yellow, orange, pink, and red varieties, with contents ranging from 0.09 to 1.55 mg/kg fresh weight (FW). Betaxanthins were not detected in the green cactus. Histidine-Bx was the prevailing betaxanthin compound in the majority of cultivars. Fifteen betaxanthins were successfully identified in the leaves of five B. vulgaris cultivars (cv.): Snow Ball, Boldor, Cylindra, Rhubarb, and Round Dark Red. Leaves of yellow beet (cv. Boldor) had the highest total betaxanthin content (20.4 mg/kg FW), while white beet (cv. Snow Ball) had the lowest one (3.43 mg/kg FW). The leaves of red cultivars had comparable betaxanthin contents, ranging from 13.4 to 18.8 mg/kg FW, similary to the yellow cultivar, indicating their potential as valuable sources of betaxanthins. There was no single dominant betaxanthin in B. vulgaris leaves. The leaves of B. vulgaris were found to be a richer source of betaxanthin than the grafted cactus G. mihanovichii, which opens up new prospects for research into the potential utilization of these compounds in the industry.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 2023, 73, 4; 375-384
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Reduction of Selected Betacyanins
    Autorzy:
    SZOT, DOMINIKA
    STARZAK, KAROLINA
    SKOPIŃSKA, ANNA
    WYBRANIEC, SŁAWOMIR
    Powiązania:
    https://bibliotekanauki.pl/articles/1034140.pdf
    Data publikacji:
    2014
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    1
    7-diazaheptamethin
    LC-MS
    betanin
    chromophore
    decarboxy-betacyanins
    reduction
    Opis:
    Betacyanins are conjugates of betalamic acid and glycosylated cyclo-DOPA which bring about the creation of a specific chromophoric system, the 1,7-dia- zaheptamethin chain. Betanin, a betacyanin pigment from red beet (Beta vulga- ris L.), as well as its decarboxylated derivatives were subjected to reduction with the use of NaBH4. The pigment structures contain a vinyl linker which is postu- lated to be protonated during reaction with NaBH4. In this study, 17-decarboxy- betanin and 2,17-bidecarboxy-betanin were obtained from betanin, which had been previously isolated from red beet root (Beta vulgaris L.). Purified pigments were tested in the presence of the reduction reagent while looking for the prod- ucts of the reaction identified by LC-MS.
    Źródło:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe; 2014, 8; 265-273
    2082-3827
    2084-977X
    Pojawia się w:
    Zeszyty Naukowe Towarzystwa Doktorantów Uniwersytetu Jagiellońskiego. Nauki Ścisłe
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of the Addition of Apple Pomace and Erythritol on the Antioxidant Capacity and Antidiabetic Properties of Shortbread Cookies
    Autorzy:
    Raczkowska, Ewa
    Wojdyło, Aneta
    Nowicka, Paulina
    Powiązania:
    https://bibliotekanauki.pl/articles/31341114.pdf
    Data publikacji:
    2024-05-17
    Wydawca:
    Instytut Rozrodu Zwierząt i Badań Żywności Polskiej Akademii Nauk w Olsztynie
    Tematy:
    apple pomace
    erythritol
    shortbread cookies
    LC-MS
    antidiabetic activity
    antioxidant activity
    Opis:
    Apple processing into juice generates vast amounts of a by-product, namely fruit pomace, which poses a serious problem for the processing industry. At the same time, fruit pomace features a high health potential. The aim of the present study was to develop recipes of 8 variants of cookies with wheat flour substituted by apple pomace (0, 10, 30 and 50% of flour weight), sweetened with sucrose and erythritol. The cookies were analyzed for their nutritional value; antioxidant capacity (ABTS•+ scavenging activity and oxygen radical absorption capacity – ORAC); the ability to inhibit α-amylase, α-glucosidase and pancreatic lipase; and consumer acceptability. In total, 13 phenolic compounds were identified in the cookies with pomace. Cookies with 50% addition of apple pomace had an approximately 8-fold higher content of dietary fibre than traditional products (without the apple pomace) and simultaneously reduced energy value (by 32.6 and 40.5 kcal/100 g of cookies sweetened with sucrose and erythritol, respectively). The antioxidant capacity of cookies was 0.032–0.316 mmol TE/100 g in the ABTS assay and 1.153–2.070 mmol TE/100 g when ORAC was determined. The IC50 enabling α-amylase and α-glucosidase inhibition ranged from 138.1 to 221.8 mg/mL and from 976.4 to 1374.9 mg/mL, respectively. The anti-lipase activity of cookies with the addition of 50% apple pomace and erythritol was the highest (IC50 of 7.3 mg/mL). Both antioxidant capacity and antidiabetic potential increased significantly with the increasing proportion of pomace in cookies. Replacing sucrose with erythritol favorably influenced the consumer assessment. The study results show that the proposed products can be a perfect alternative to traditional sweet snack products, especially for consumers with diet-related diseases. The feasibility of using waste raw materials, which are a challenge to the food industry, has been proven as well.
    Źródło:
    Polish Journal of Food and Nutrition Sciences; 2024, 74, 2; 147-161
    1230-0322
    2083-6007
    Pojawia się w:
    Polish Journal of Food and Nutrition Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chromatographic fractionation of betacyanins from flowers of Gomphrena globosa
    Autorzy:
    Szot, D.
    Starzak, K.
    Skopińska, A.
    Wybraniec, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/115427.pdf
    Data publikacji:
    2015
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    betacyanins
    Gomphrena globosa
    plant pigments
    LC-MS
    betacyjaniny
    Gomfrena kulista
    barwniki roślinne
    Opis:
    In this study, a chromatographic fractionation of betacyanin pigments from extract of purple Gomphrena globosa petals was performed by preparative high performance liquid chromatography (prep-HPLC). The particular betacyanins in each collected fractions were tentatively identified by chromatography with optical amd mass spetrometric detection (LC-DAD-ESI-MS). Betacyanins are natural pigments, which are confirmed to have an antioxidant activity. It was reported that betacyanins can prevent civilization diseases, because of an ability of free radicals scavenging. Betacyanins are present in tissue of plants from Amaranthaceae family, e.g. in petals of Gomphrena globosa. Preparative separation of these particular pigments is difficult, because they are structurally very similar to each other and tend to coelute. However, in this study, fractions, containing a dominant amount of the principal pigments of Gomphrena globosa inflorescences, were obtained successfully. In the first fractions, two isomeric pigments assigned to gomphrenin I and isogomphrenin I were detected. The fractions of significant amounts of gomphrenin III and isogomphrenin III were obtained separately with sufficient purity. The presence of other, minor gomphrenin-type betacyanins were also confirmed as: gomphrenin II, cis-isomer of gomphrenin II and sinapoyl-gomphrenin I as well as their 15S-diastereomers. Moreover, in other fractions, various unknown pigments were detected.
    Źródło:
    Challenges of Modern Technology; 2015, 6, 2; 19-23
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of phenolic profile and in vitro antioxidant capacity of Sorbus domestica L. leaf samples from Poland and Croatia
    Autorzy:
    Rutkowska, M.
    Dybicka, M.
    Olszewska, M.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2117865.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    antioxidant activity
    extract quality
    LC-MS
    leaves
    polyphenolic composition
    Sorbus
    domestica L.
    Opis:
    This work presents a comparative analysis of the phenolic composition (UHPLC-PDA-ESI-MS 3 , HPLC-PDA- fingerprint, UV-spectrophotometric methods) and antioxidant activity (DPPH, FRAP) of leaf samples from two vegetation seasons of a medicinal and dietary plant Sorbus domestica growing in its natural habitat (Croatia, C) and cultivated in Poland (P). The samples from both sources were rich in structurally diverse polyphenols (44 analytes; P: 73.4–76.6 and C: 98.3–106.7 mg GAE/g dry leaves) including the dominating flavan-3-ols and flavonoids. The greatest qualitative and quantitative differences were observed for flavonoids (P: 14.3–20.3%; C: 27.5–34.1% of polyphenols) – in the Polish samples flavonoid diglycosides predominated, in the Croatian samples the contents of both monoglycosides and diglycosides were similar. In the case of dry methanolic extracts, despite the higher extraction efficiency obtained for the Croatian samples (32–36% vs 23–24%), the quality of the extracts was comparable, both in terms of the total phenolic content (P: 269.4–280.0; C: 297.6–304.4; mg GAE/g dry extract) and antioxidant activity parameters (DPPH, EC 50 , μg/mL. P: 10.5–10.9, C: 10.0–10.3; and FRAP, mmol Fe 2+/g, P: 6.64–7.13, C: 7.06–7.11). As a result, the study confirmed the influence of environmental conditions on the phenolic profile and antioxidant capacity of S. domestica leaves, as well as showed that despite some differences, plant materials from both Poland and Croatia might be suitable for production of natural health products.
    Źródło:
    Acta Biologica Cracoviensia. Series Botanica; 2019, 61, 1; 63-73
    0001-5296
    Pojawia się w:
    Acta Biologica Cracoviensia. Series Botanica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Profiles of products generated during betacyanins reduction by 2-methylpyridine borane
    Autorzy:
    Szot, D.
    Starzak, K.
    Skopińska, A.
    Wybraniec, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/115550.pdf
    Data publikacji:
    2015
    Wydawca:
    Fundacja na Rzecz Młodych Naukowców
    Tematy:
    betanina
    neobetanina
    betacyjaniny
    redukcja
    2-2-metylopirydyna boranu
    LC-MS
    betanin
    neobetanin
    betacyanins
    reduction
    2-methylpyridine borane
    Opis:
    Betanin and neobetanin (14,15-dehydrogenated betanin), natural betacyanin pigments were subjected to a gentle reduction by 2-methylpyridine borane. During each reduction reaction, the visible spectra were collected. The results were tentatively confirmed by LC-DAD-ESI-MS. In comparison to a previous study with sodium borohydride, various types of products were obtained. Depending on pH, one or two double bonds are reduced. Interestingly, a preliminary decarboxylation of the pigments is also noticed. Betacyanins are plant red-violet pigments applied as colorants in food and pharmaceutical industry which are proven to have an antioxidant activity. They are confirmed to be beneficial for human health as free radical scavengers. Therefore, betacyanins can prevent from many diseases which would be induced by an excessive accumulation of free radicals in the human body. There are more and more reports of their pro-health properties. However, the mechanism of betacyanins oxidation remains not completely elucidated. It is postulated that the rearrangement in the chromophoric system is a significant part of this mechanism. The products obtained as a result of reduction of betanin and neobetanin can be useful in oxidation investigations. Betacyanins with partially destroyed chromophoric system by reduction of the double bonds would by perfect models for further studies on their oxidation.
    Źródło:
    Challenges of Modern Technology; 2015, 6, 4; 19-22
    2082-2863
    2353-4419
    Pojawia się w:
    Challenges of Modern Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Addition of bisulfite ions to methyl vinyl ketone and methacrolein relevant to atmospheric processes
    Przyłączanie anionów wodorosiarczynowych do ketonu metylowo-winylowego i metakroleiny o znaczeniu dla procesów atmosferycznych
    Autorzy:
    Szmigielski, R.
    Rudziński, K. J.
    Powiązania:
    https://bibliotekanauki.pl/articles/953669.pdf
    Data publikacji:
    2013
    Wydawca:
    Towarzystwo Chemii i Inżynierii Ekologicznej
    Tematy:
    proces atmosferyczny
    wtórny aerosol atmosferyczny
    stałe szybkości
    spektrometria mas
    detekcja elektrosprej
    LC MS
    izopren
    metakroleina
    keton metylowo-winylowy
    ditlenek siarki
    addycja wodorosiaczynu
    związki karbonylowe
    atmospheric processes
    secondary organic aerosols
    rate constants
    electrospray mass spectrometry
    LC-MS
    isoprene
    methacrolein
    methyl vinyl ketone
    sulfur dioxide
    bisulfite addition
    carbonyls
    Opis:
    Metakroleina oraz keton metylowo-winylowy są bardzo reaktywnymi zawiązkami karbonylowymi, które odgrywają istotną rolę w procesach atmosferycznych, w tym – w tworzeniu pyłów zwieszonych w powietrzu. Obydwa związki są produktami utleniania izoprenu – węglowodoru emitowanego do atmosfery w ogromnych ilościach. W pracy przedyskutowano mechanizmy chemiczne zaniku metakroleiny oraz ketonu metylowo-winylowego w wyniku addycji anionu wodorosiarczanowego w rozcieńczonych roztworach wodnych, odzwierciedlające procesy zachodzące w kroplach wód atmosferycznych w rejonach o znaczącym stężeniu ditlenku siarki. W pracy pokazano, że reakcje addycji anionu wodorosiarczynowego do badanych związków karbonylowych prowadzą do tworzenia pierwotnych i wtórnych hydroksysulfonianów, które mogą uczestniczyć w tworzeniu aerozoli atmosferycznych. Wyznaczono stałe szybkości wszystkich reakcji w mechanizmie addycji. Pierwotna addycja anionów wodorosiarczynowych do metakroleiny okazała się znacznie szybsza niż do ketonu metylowo-winylowego (stałe szybkości kMAC1f = 8 i kMVK1f = 0.18 mol–1 dm3 s–1 w temperaturach pokojowych). Stała szybkości addycji do cząsteczki metakroleiny była dziesięciokrotnie większa od stałej opublikowanej w literaturze. Analiza kinetyczna i badania produktów reakcji za pomocą spektrometrii mas wykazały, że w przypadku każdego z badanych związków karbonylowych dominującym produktem reakcji był C4 alfa-hydroksysulfonian (addukt pierwotny), natomiast C4 hydroksydwusulfonian (addukt wtórny, diaddukt) powstawał w ilościach śladowych. Pierwotna addycja anionów wodorosiarczynowych do metakroleiny i ketonu metylowo-winylowego może mieć znaczenie w konwersji reaktywnych związków karbonylowych w atmosferze i powinna być uwzględniana w badaniach dotyczących rejonów o znaczącej obecności ditlenku siarki, gwarantującej wysokie stężenia anionów wodorosiarczynowych w wodach atmosferycznych.
    Methacrolein and methyl vinyl ketone are highly reactive carbonyls that play a pivotal role in the formation of secondary organic aerosols in the Earth’s atmosphere. Both carbonyls are the major products of isoprene oxidation. We show that among the atmospheric sinks of methacrolein and methyl vinyl ketone, the aqueous-phase addition of bisulfite anions to their molecules can be relevant under polluted conditions with the increased presence of sulfur dioxide. We demonstrate that aqueous-phase reactions of methyl vinyl ketone and methacrolein with bisulfite anions lead to the formation of primary and secondary organic hydroxysulfonates which currently are not included in the atmospheric chemistry modelling, but can be relevant in mechanisms explaining the formation and growth of the secondary organic aerosols from atmospheric carbonyls. The rate constants for all aqueous-phase reactions involved were determined. The primary addition of bisulfite anions to methacrolein was found significantly faster than that to methyl vinyl ketone, with rate constants kMAC1f = 8 and kMVK1f = 0.18 mol–1 dm3 s–1 at room temperatures, respectively. The rate constant for the bisulfite addition to methacrolein was ten times faster than reported in the literature. The kinetic and mass spectrometric analyses revealed that in both cases, the dominating product was the C4 alpha-hydroxysulfonate (a primary adduct), while the C4 alpha-hydroxy disulfonate (a secondary adduct or diadduct) was produced only in trace quantities. The primary addition of bisufite anions to methacrolein and methyl vinyl ketone should be considered in atmospheric studies relevant to areas with enhanced presence of sulfur dioxide providing sufficiently high concentrations of bisulfite ions in atmospheric waters.
    Źródło:
    Ecological Chemistry and Engineering. A; 2013, 20, 9; 935-949
    1898-6188
    2084-4530
    Pojawia się w:
    Ecological Chemistry and Engineering. A
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Metabolomic analysis of biological material using LC-MS in the quest for urinary system cancer biomarkers - review
    Autorzy:
    Szuberla, Wiktoria
    Wrona, Maria
    Powiązania:
    https://bibliotekanauki.pl/articles/31342652.pdf
    Data publikacji:
    2024
    Wydawca:
    Politechnika Rzeszowska im. Ignacego Łukasiewicza. Oficyna Wydawnicza
    Tematy:
    mass spectrometry
    metabolomics
    LC-MS
    kidney cancer
    renal cell carcinoma
    bladder cancer
    spektrometria mas
    metabolomika
    rak nerki
    rak nerkowokomórkowy
    rak pęcherza moczowego
    Opis:
    Renal cell carcinoma (RCC) and bladder cancer (BC) are among the most frequently diagnosed urinary system cancers worldwide. They are characterized by high mortality and recurrence rates. In response to the rising incidence and mortality rates, scientists are exploring innovative diagnostic and therapeutic methods. Metabolomics, which analyzes metabolite levels, may enable early diagnosis and monitoring of therapy progress. Compared to other omics technologies, it focuses on the outcomes of metabolite activity, providing a unique perspective on processes occurring in cancer cells. Metabolomic analyses utilize techniques such as mass spectrometry. These methods allow the identification of biomarkers and precise determination of the chemical composition of biological samples. However, the most commonly used method is liquid chromatography-mass spectrometry (LC-MS), which enables the most comprehensive screening of cancer metabolomes. Recent studies show significant progress in recognizing characteristic metabolites associated with urological cancers, although this area remains partially unexplored. Research on circulating metabolites, especially those present in easily accessible samples like blood or urine, demonstrates promising potential in clinical practice. Study results reveal differences in metabolic profiles between various stages of cancer development, which may have clinical significance. The future of this field involves an increasing number of clinical cohorts, standardization of sample preparation, and further improvements in instrument sensitivity and speed. LC-MS-based metabolomics has the potential to contribute to the improvement of diagnostics, therapy, and the quality of life of patients with some urological cancers. However, challenges, such as the lack of uniform methodologies and understanding of metabolite determinants, require further research and innovation.
    Źródło:
    Chemical Technology & Biotechnology; 2024, 4; 70-75
    2720-6793
    Pojawia się w:
    Chemical Technology & Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-11 z 11

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