- Tytuł:
- Acid alpha-galactosidase is involved in D-chiro-inositol accumulation during tartary buckwheat germination
- Autorzy:
-
Jia, C.-F.
Hu, W.-H.
Chang, Z.
Gao, H.-L. - Powiązania:
- https://bibliotekanauki.pl/articles/59199.pdf
- Data publikacji:
- 2015
- Wydawca:
- Polskie Towarzystwo Botaniczne
- Tematy:
-
alpha-galactosidase
D-chiro-inositol
accumulation
tartary buckwheat
Fagopyrum tataricum
duckwheat zob.tartary buckwheat
India buckwheat zob.tartary buckwheat
India wheat zob.tartary buckwheat
green buckwheat zob.tartary buckwheat
germination
gene expression - Opis:
- Tartary buckwheat seed and especially its sprouts are rich in D-chiroinositol (DCI). The research was to evaluate when DCI was most accumulated in tartary buckwheat sprouts. In addition, we explored the activity and expression pattern of α-galactosidase during tartary buckwheat seed germination. The results showed that DCI contents steadily increased at early stage of germination and reached the highest level of 33.42 μg/seed at 24 h during the 72 h trail. However, the total fagopyritol contents sharply decreased from 214.6 μg/seed to 46 μg/seed at the end of the germination. The activity of acid α-galactosidase increased gradually to the peak of 0.36 nkat/seed at 24 h after the primed seed imbibition. We cloned the gene fragment of α-galactosidase in tartary buckwheat for the first time. The deduced amino acid sequence is 93% identical to that of P. vulgaris. The quantitative PCR result of gene expression pattern was consistent with its enzyme activity during seed germination.
- Źródło:
-
Acta Societatis Botanicorum Poloniae; 2015, 84, 1
0001-6977
2083-9480 - Pojawia się w:
- Acta Societatis Botanicorum Poloniae
- Dostawca treści:
- Biblioteka Nauki
Artykuł