- Tytuł:
- Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.
- Autorzy:
-
Perlińska-Lenart, Urszula
Kurzątkowski, Wiesław
Janas, Piotr
Kopińska, Agnieszka
Palamarczyk, Grażyna
Kruszewska, Joanna - Powiązania:
- https://bibliotekanauki.pl/articles/1041477.pdf
- Data publikacji:
- 2005
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
glycosylation
protein secretion
DPM1 gene
Aspergillus
dolichylphosphate mannose synthase - Opis:
- O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion.
- Źródło:
-
Acta Biochimica Polonica; 2005, 52, 1; 195-205
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł