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Tytuł:
Zmiany patologiczne wątroby w inwazji przywry Fasciola hepatica
Autorzy:
Gajewska, A.
Smaga-Kozłowska, K.
Wiśniewski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2146376.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przywry
Fasciola hepatica
parazytologia
pasozyty
inwazja pasozytnicza
watroba
zmiany patologiczne
Opis:
Pathological changes of liver in infection of Fasciola hepatica. Fasciolosis caused by Fasciola hepatica is a hepatic parasitic infection that affects numerous mammal species, mainly ruminants, in several countries of Europe, Asia and America. The economic significance of fasciolosis is mainly due to direct losses caused by a decrease of weight rate, milking capacity and the confiscation of altered livers in slaughterhouse. In this review we present mechanisms of damaging of host liver during invasion of Fasciola hepatica. Parasites digest hepatic tissue and cause extensive parenchymal destruction with intensive haemorrhagic lesions and immunological reactions. Mechanical liver damage is due to migrating juvenile fluke. Macerated hepatic cells have been observed inside the oral sucker and pharynx. Moreover, the process of ulceration of liver tissue was observed in regions adjacent to the spiny body of fluke tegument. On the other hand, the injury of the liver can be induced chemically by factors produced or induced by the fluke. Probably, fluke proteases and other tissue-degrading enzymes can be responsible for negative effect on liver parenchyma. There is no evidence to link fluke-enzymes with tissue penetration. The consequences of liver damage resulting from the migrating flukes compromises liver function which is reflected in changes of plasma protein concentration (albumin, globulin). Additianally, changes of levels of hepatic enzymes released into the blood as a result of damage of liver tissue are used to monitor the progress of the infection in a variety of Fasciola hepatica hosts and as a sensitive diagnostic aid in field infection.
Źródło:
Wiadomości Parazytologiczne; 2005, 51, 2; 115-123
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
NIEKTÓRE SPECYFICZNE I NIESPECYFICZNE FOSFATAZY W SPOROCYSTACH FASCIOLA HEPATICA. III. ENZYMY ZWIĄZANE Z GLIKOLIZĄ
Autorzy:
Humiczewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148926.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Wiadomości Parazytologiczne; 1997, 43, 4; 411-423
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood leucocyte responses in rats vaccinated with cDNA encoding glutathione-s-transferase of Fasciola hepatica
Autorzy:
Wedrychowicz, H
Jedlina-Panasiuk, L.
Szymanski, P.
Bienkowska-Szewczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/839010.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glutathione s-transferase
blood
Fasciola hepatica
leucocyte level
vaccinated rat
cDNA
rat
leucocyte
Opis:
Changes in blood leucocyte levels were investigated in Spraque-Dowley rats vaccinated with cDNA or protein of glutathione S-transferase (GST) of F. hepatica and subsequently challenged with metacercariae of the liver fluke. The analysis of the leucocyte responses measured in vaccinated rats suggests that the form of antigen used for vaccination intluenced dynamics of white blood cell response to the fluke infection. The most clear differences were observed in neutrophil and eosinophil levels. The weakest reaction of these cells to the challenge infection was observed in rats vaccinated twice with cDNA. In contrast, in rats which received the first antigen dose as cDNA and the second vaccination with GST protein, both neutrophil and eosinophil responses were much higher, especially at 5 and 9 WAI.
Badano reakcję leukocytów krwi szczurów Spraque-Dowley immunizowanych cDNA lub białkiem transferazy S-glutationowej (GST) F. hepatica na inwazję metacerkarii tej przywry. Zwierzęta grupy 3 otrzymały pierwszą dawkę antygenu w formie cDNA a drugą w postaci białka. Próbki krwi do badań pobrano w dniu zarażenia a następne w 1, 5 i 9 tygodniu po zarażeniu (tpz). U szczurów szczepionych cDNA zaobserwowano statystycznie istotne obniżenie liczby limfocytów poczynając od 1 tpz. Największe różnice zaobserwowano w reakcji granulocytów. Szczury immunizowane dwukrotnie cDNA wykazywały najmniejszą eozynofilię zaś u szczurów grupy 3, wystąpiła w 9 tpz eozynofilia i neutrofilia silniej wyrażona niż u szczurów nie immunizowanych.
Źródło:
Annals of Parasitology; 2001, 47, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wplyw pasozytowania larw Fasciola hepatica na ultrastrukture watrobotrzustki Galba truncatula L.
Autorzy:
Humiczewska, M
Powiązania:
https://bibliotekanauki.pl/articles/841516.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
Fasciola hepatica
Galba truncatula
slimaki
parazytologia
inwazja pasozytnicza
ultrastruktura
larwy
watrobotrzustka
Opis:
Ultrastructure of the digestive gland has been studied in G. truncatula, uninfected and infected with F. hepatica larvae. The digestive gland of G. truncatula consist of seccretory-resorption cells and calcareous cells. On the 20 day post infection the damage to the secretory cells were small and did not inhibit their activity. On the 60 day post infection the gland structure was totally altered: reduced height of secretory cells, lack of Golgi apparatus, myelin-like degradation of mitochondria, reduction and desintegrating of rough endoplasmic reticulum. These damage of secretory and calcareous cell to bring about a total inhibition of their secretory activities.
Źródło:
Annals of Parasitology; 2001, 47, 4; 647-649
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The prevalence of Fasciola hepatica L. infection in cattle in the Lublin province in the years 2009-2012
Autorzy:
Kozlowska-Loj, J.
Loj-Maczulska, A.
Powiązania:
https://bibliotekanauki.pl/articles/6671.pdf
Data publikacji:
2013
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
prevalence
Fasciola hepatica
animal infection
cattle
slaughtered animal
Lublin province
2009-2012 period
Źródło:
Annals of Parasitology; 2013, 59, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ pasożytowania larw Fasciola hepatica na ultrastrukturę wątrobotrzustki Galba truncatula L.
Autorzy:
Humiczewska, M.
Powiązania:
https://bibliotekanauki.pl/articles/2148132.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
pasozyty zwierzat
Fasciola hepatica
Galba truncatula
slimaki
parazytologia
inwazja pasozytnicza
ultrastruktura
larwy
watrobotrzustka
Opis:
Ultrastructure of the digestive gland has been studied in G. truncatula, uninfected and infected with F. hepatica larvae. The digestive gland of G. truncatula consist of seccretory-resorption cells and calcareous cells. On the 20 day post infection the damage to the secretory cells were small and did not inhibit their activity. On the 60 day post infection the gland structure was totally altered: reduced height of secretory cells, lack of Golgi apparatus, myelin-like degradation of mitochondria, reduction and desintegrating of rough endoplasmic reticulum. These damage of secretory and calcareous cell to bring about a total inhibition of their secretory activities.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 647-649
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Some specific and non-specific phosphatases in the sporocyst of Fasciola hepatica. 3. Enzymes associated with glycolysis
Autorzy:
Humiczewska, M
Powiązania:
https://bibliotekanauki.pl/articles/837871.pdf
Data publikacji:
1997
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glucose-6-phosphatase
aldolase
enzyme
sporocyst
glycolytic enzyme
Fasciola hepatica
phosphatase
hexokinase
Opis:
Using histochemical and cytophotometric methods, enzymes associated with anaerobie glycolysis: glucose-6-phosphatase, hexokinase, and aldolase in the developing sporocysts of Fasciola hepatica were studied. Highest activity of these enzymes was found in the germ balls in the sporocysts, at all phases of their development, which is related to intensive proliferation and differentiation of the embryos developing inside the germ balls.
Źródło:
Annals of Parasitology; 1997, 43, 4
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of Fasciola hepatica L. infection in cattle in the Lublin province (Poland) in the years 2005-2008
Autorzy:
Kozlowska-Loj, J.
Powiązania:
https://bibliotekanauki.pl/articles/837100.pdf
Data publikacji:
2011
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
prevalence
Fasciola hepatica
animal infection
cattle
slaughtered animal
Polska
2005-2008 period
Lublin region
Opis:
Prevalence of Fasciola hepatica L. infection among cattle slaughtered in the Lublin province in the years 2005–2008 was evaluated. The study draws its data from annual reports of the Veterinary Inspectorate in Lublin. The results of the study are as follows: the prevalence of Fasciola hepatica infection in the cattle of the Lublin province between 2005 and 2008 amounts to 21.24%.
Źródło:
Annals of Parasitology; 2011, 57, 2
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ inwazji Fasciola hepatica na wybrane parametry jakościowe mleka u krów
Autorzy:
Michalski, M.
Powiązania:
https://bibliotekanauki.pl/articles/2147693.pdf
Data publikacji:
2002
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
Fasciola hepatica
krowy mleczne
parametry jakosciowe
motylica watrobowa
parazytologia
mleko krowie
pasozyty
inwazja pasozytnicza
Opis:
The influence of Fasciola hepatica invasion on the assorted milk quality parameten in cows. The investigations of the influence of Fasciola hepatica invasion on the assorted parameters of milk quality were performed in cows. Mean protein content in the milk from infected animals ammounted 3.60% and was higher than in the milk from uninfected cows (3.30%). Similar tendency was observed in the case of the fat (5.08% and 3.91 %) and lactose (4.36% and 4.34%). The count of somatic cells in milk from infected cows was lower than in milk from uninfected animals (356.07 and 1776.92). It can indicate immunosupressive activity of Fasciola hepatica on the host.
Źródło:
Wiadomości Parazytologiczne; 2002, 48, 2; 225-229
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad wystepowaniem Fasciola hepatica na wybranych obszarach Polski w oparciu o metody molekularne i serologiczne
The occurrence of Fasciola hepatica in chosen regions of Poland based on molecular and serological methods
Autorzy:
Kozak-Cieszczyk, M
Powiązania:
https://bibliotekanauki.pl/articles/839741.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przywry
Fasciola hepatica
choroby czlowieka
Galba truncatula
zywiciele posredni
parazytologia
pasozyty
choroby zwierzat
Opis:
Fasciolosis, caused by the liver fluke (Fasciola hepatica) is an important issue for both human and animal health. The disease evokes economic losses which are a consequence of impaired animal productivity leading to higher costs of meat and milk production, as well as liver condemnation. The goals of this thesis were to: (1) elaborate a molecular method - PCR for the detection of F. hepatica DNA in intermediate and definite hosts; (2) estimate the usefulness of a recombinated cysteine proteinase produced in E. coli in the form of inclusive bodies in serological diagnosis of F. hepatica infection in definite hosts, using an enzyme-linked immunosorbent assay (ELISA); (3) conduct field research on the prevalence of infection among intermediate and definitive hosts (cattle) in chosen regions of Poland, utilizing the elaborated methods. Based on the results obtained in this study, it was established that it is possible to detect F. hepatica DNA in the feces of definite hosts with the elaborated PCR method. The amplification of a 124 base pair tandem repeat allows the detection of fluke larval stages in intermediate hosts within 12 hours of exposure and F. hepatica infection in definite hosts (by the 5th week in rats, 8th week in sheep and 10th week in cattle). Therefore, the PCR test is more sensitive than traditional microscopic methods. Furthermore, it was determined that, the recombinated cysteine proteinase in the form of inclusive bodies, after solubillization exhibits antigenic properties of the native protein and the ELISA method based on this antigen may be useful as a tool for diagnosing fasciolosis in sheep and cattle, in both serum and milk samples. The test achieves a greater sensitivity and specificity than an ELISA based on native excretory-secretory antigens. The results of field research indicate that Fasciola hepatica is a frequent parasite of cattle in central and eastern Poland. The mean prevalence was 34.86% (±16.95) in all studied areas. The prevalence among intermediate hosts varied greatly (0–100%). The elaborated tests were proved to be valuable, mutually complementing diagnostic tools, applicable to different epidemiological situations.
Źródło:
Annals of Parasitology; 2006, 52, 2; 137-139
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania nad występowaniem Fasciola hepatica na wybranych obszarach Polski w oparciu o metody molekularne i serologiczne
The occurrence of Fasciola hepatica in chosen regions of Poland based on molecular and serological methods
Autorzy:
Kozak-Cieszczyk, M.
Powiązania:
https://bibliotekanauki.pl/articles/2144329.pdf
Data publikacji:
2006
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
przywry
Fasciola hepatica
choroby czlowieka
Galba truncatula
zywiciele posredni
parazytologia
pasozyty
choroby zwierzat
Opis:
Fasciolosis, caused by the liver fluke (Fasciola hepatica) is an important issue for both human and animal health. The disease evokes economic losses which are a consequence of impaired animal productivity leading to higher costs of meat and milk production, as well as liver condemnation. The goals of this thesis were to: (1) elaborate a molecular method - PCR for the detection of F. hepatica DNA in intermediate and definite hosts; (2) estimate the usefulness of a recombinated cysteine proteinase produced in E. coli in the form of inclusive bodies in serological diagnosis of F. hepatica infection in definite hosts, using an enzyme-linked immunosorbent assay (ELISA); (3) conduct field research on the prevalence of infection among intermediate and definitive hosts (cattle) in chosen regions of Poland, utilizing the elaborated methods. Based on the results obtained in this study, it was established that it is possible to detect F. hepatica DNA in the feces of definite hosts with the elaborated PCR method. The amplification of a 124 base pair tandem repeat allows the detection of fluke larval stages in intermediate hosts within 12 hours of exposure and F. hepatica infection in definite hosts (by the 5th week in rats, 8th week in sheep and 10th week in cattle). Therefore, the PCR test is more sensitive than traditional microscopic methods. Furthermore, it was determined that, the recombinated cysteine proteinase in the form of inclusive bodies, after solubillization exhibits antigenic properties of the native protein and the ELISA method based on this antigen may be useful as a tool for diagnosing fasciolosis in sheep and cattle, in both serum and milk samples. The test achieves a greater sensitivity and specificity than an ELISA based on native excretory-secretory antigens. The results of field research indicate that Fasciola hepatica is a frequent parasite of cattle in central and eastern Poland. The mean prevalence was 34.86% (±16.95) in all studied areas. The prevalence among intermediate hosts varied greatly (0–100%). The elaborated tests were proved to be valuable, mutually complementing diagnostic tools, applicable to different epidemiological situations.
Źródło:
Wiadomości Parazytologiczne; 2006, 52, 2; 137-139
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Blood leucocyte responses in rats vaccinated with cDNA encoding glutathione-s-transferase of Fasciola hepatica
REAKCJA LEUKOCYTÓW KRWI SZCZURÓW SZCZEPIONYCH cDNA TRANSFERAZY s-GLUTATIONOWEJ FASCIOLA HEPATICA
Autorzy:
Wędrychowicz, H.
Jedlina-Panasiuk, L.
Szymański, P.
Bieńkowska-Szewczyk, K.
Powiązania:
https://bibliotekanauki.pl/articles/2148264.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
glutathione s-transferase
blood
Fasciola hepatica
leucocyte level
vaccinated rat
cDNA
rat
leucocyte
Opis:
Changes in blood leucocyte levels were investigated in Spraque-Dowley rats vaccinated with cDNA or protein of glutathione S-transferase (GST) of F. hepatica and subsequently challenged with metacercariae of the liver fluke. The analysis of the leucocyte responses measured in vaccinated rats suggests that the form of antigen used for vaccination intluenced dynamics of white blood cell response to the fluke infection. The most clear differences were observed in neutrophil and eosinophil levels. The weakest reaction of these cells to the challenge infection was observed in rats vaccinated twice with cDNA. In contrast, in rats which received the first antigen dose as cDNA and the second vaccination with GST protein, both neutrophil and eosinophil responses were much higher, especially at 5 and 9 WAI.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 551-557
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The construction of recombinant vectors for a DNA vaccination of rats against fasciolosis
Autorzy:
Kofta, W.
Wedrychowicz, H.
Powiązania:
https://bibliotekanauki.pl/articles/838715.pdf
Data publikacji:
1998
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
parasite
vaccination
cattle
economic loss
fluke
fasciolosis
mRNA
Fasciola hepatica
liver
cysteine proteinase
cDNA
rat
Źródło:
Annals of Parasitology; 1998, 44, 3
0043-5163
Pojawia się w:
Annals of Parasitology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Ekstensywność inwazji Fasciola hepatica L. u bydła na terenie woj. lubelskiego w latach 1993-2001
EXTENSIVITY OF INFECTION OF FASCIOLA HEPATICA L. IN CATTLE IN LUBLIN’S VOIVODSHIP IN THE YEARS 1993-2001
Autorzy:
Deryło, A.
Kozłowska-Łój, J.
Szilman, P.
Najda, N.
Seniuk, A.
Wasilewski, K.
Powiązania:
https://bibliotekanauki.pl/articles/2148213.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Parazytologiczne
Tematy:
bydlo
pasozyty zwierzat
Fasciola hepatica
motylica watrobowa
ekstensywnosc inwazji
woj.lubelskie
inwazja pasozytnicza
parazytologia weterynaryjna
Opis:
Extensivity of infection of Fasciola hepatica L. among cattle in Lublin's voivodship from 1993-2001. Aim of the work was to determine extensitivity of appearance of hepatica among catlle pul to slaughter in slaughtershouses in Lublin's voivodship from 1993 to 2001. Substance for the work was braught from the annual reports of official examination of slaughtered animals, reached from both main Inspectorate (department) of Veterinary in the Ministry of Agriculture and from Regional Institute of Veterinar's Hygiene in Lublin. Results of researches are as follow: extensivity of infection of Fasciola hepatica among cattle in Lublin and voivodship between 1993-1999 oscillates about 5%. In 2000 extensivity of invasion reached 10,9 %.
Źródło:
Wiadomości Parazytologiczne; 2001, 47, 4; 775-778
0043-5163
Pojawia się w:
Wiadomości Parazytologiczne
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 38

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