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Wyszukujesz frazę "bioassay" wg kryterium: Temat


Wyświetlanie 1-6 z 6
Tytuł:
"Battery of Bioassays" for Diagnostics of Toxicity of Natural Water when Pollution with Aluminum Compounds
Autorzy:
Olkova, Anna
Berezin, Grigory
Powiązania:
https://bibliotekanauki.pl/articles/1839077.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
aluminum
battery of bioassay
Daphnia magna
Ceriodaphnia affinis
Paramecium caudatum
Escherichia coli
Opis:
The article presents the results of an experimental comparison of the sensitivity of biotests using Daphnia magna Straus, Ceriodaphnia affinis Lilljeborg, Paramecium caudatum Ehrenberg, and Escherichia coli Migula (strain M-17) to water pollution with aluminum compounds. The research was carried out under simulated conditions: the model toxicant was aluminum sulfate Al2(SO4)3∙18H2O, the concentration range per Al was 0.04–2.8 mg/dm3, and the pH of the tested waters was close to the neutral level of 7.2–7.8. The bioluminescence of E. coli significantly decreased at an Al concentration of 0.8 mg/dm3 (toxicity index was 93.3±1.2, which refers to a high level of toxicity). The reaction of P. caudatum was weaker: a high level of toxicity was achieved at an Al concentration of 2.8 mg/dm3. These doses did not cause the death of D. magna and C. affinis in short-term experiments (28 and 96 hours, respectively). However, in the tests for the chronic toxicity of aluminum, we showed that the doses of 0.8 and 2 mg/dm3 Al cause high death of individuals (more than 50%) and a significant decrease in the number of offspring. The range of sensitivity of the bioassay methods to water pollution with aluminum turned out to be as follows: bioassay for the bioluminescence of E. coli > bioassay for the changes in chemotaxis of P. caudatum > bioassay for the changes in fertility of D. magna > bioassay for the changes in fertility of C affinis.
Źródło:
Journal of Ecological Engineering; 2021, 22, 2; 195-199
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Bioremediation Possibilities of Oil-Contaminated Soil by Biosurfactant Based on Bacillus Strain
Autorzy:
Nedoroda, Vladyslav
Trokhymenko, Ganna
Magas, Nataliya
Powiązania:
https://bibliotekanauki.pl/articles/2173345.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
biodegradation
bioremediation
biosurfactant
petroleum
phytotoxicity
soil
Opis:
This paper represents the results of the investigation of soil phytotoxicity with a high level of oil pollution. The artificially contaminated soils were exposed to an oil-destroying biosurfactant based on the strains of Bacillus amyloliquefaciens and Bacillus subtilis. The main objective of the work was to evaluate the effectiveness of a consortium of microorganisms in reducing the phytotoxicity of soils with high levels of oil contamination. The determination was performed on the reactions of test organisms to the pollution of the soil environment. The test system in the experiment comprised Sorghum bicolor subsp. Drummondii, Phleum pretense, Galéga officinális, Trifolium pretense, Medicágo sativa, and Sinapis arvensis. The variety of the applied indicator plants has provided a comprehensive analysis of the results of oil destruction and allowed an assessment of the sensitivity of the seeds of each species to toxic substances.
Źródło:
Journal of Ecological Engineering; 2022, 23, 8; 49--55
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Behavioral and Lethal Effects of La Salt and a Mixture of Cu and La Salt on Daphnia magna Straus
Autorzy:
Olkova, Anna Sergeewna
Sysolyatina, Maria
Powiązania:
https://bibliotekanauki.pl/articles/2105282.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
lanthanum
copper
bioassay
Daphnia magna
motor activity
joint effect
synergism
potentiation
Opis:
The study of joint effects of REE and HM is relevant, since they are often satellite deposits, their areas of application are similar, and an increase in concentrations of elements of these groups in the areas that are not places of their extraction and enrichment is proven. The purpose of this work was to find out the pre-lethal and lethal effects of La, Cu and their equimolar mixtures in tests for Daphnia magna Straus. Bioassays of artificially polluted natural waters, initially free of toxic elements, was carried out. In bioassays on the mortality of D. magna in the space of 96 hours it was found that acute toxicity of copper sulfate solutions is observed at the calculated concentration of Cu2+ 0.1 mg/L (0.0016 mmol/L), and the acute toxicity of lanthanum sulfate is when the dose of La3+ is equal to 50 mg/L (0.36 mmol/L). In the solutions comprising mixtures of Cu and La salts (1:1 calculated using metals), the concentrations of which are equimolar to the investigated solutions of copper sulfate, the mortality of D. magna begins in the solution containing 10 times less toxic elements. It was found that 25% of individuals died in the variant “0.00016 mmol/L”, the mortality of 100% of individuals was at the total metal concentration of 0.0008 mmol/L. The solutions containing La (0.072–0.72 mmol/L) and Cu (0.00016–0.0016 mmol/L) naturally inhibit the motor activity of D. magna by 1.3–5.3 times and 1.2–1.9 times in 1 hour and 1.7–2.8 and 1.4–2.2 times in 24 hours, respectively. The solutions containing mixtures of Cu and La salts inhibited the motor activity of D. magna in the same way as copper sulfate solutions with the Cu2+ concentrations equimolar “Cu2+ + La3+”. Therefore, when testing the solutions with the same molar concentrations of Cu2+ and the mixture of “Cu2+ + La3+” it was shown that La potentiates the pre-lethal effect of Cu to the level of individual effects of Cu. The additions of La salt to the solutions containing pre-lethal doses of Cu lead to lethal effects of such mixtures for D. magna.
Źródło:
Journal of Ecological Engineering; 2022, 23, 6; 245--252
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Use of the Triad Approach to Ecological Assessment of the State of a Reservoir Receiving Wastewater From a Thermal Power Plant
Autorzy:
Mahanova, Elena
Powiązania:
https://bibliotekanauki.pl/articles/1839092.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
thermal power plant
chemical analysis of water
bioassay
bioindication
pollution
trophicity
Opis:
Thermal power plants (TPP) belong to the enterprises that accompany the development of any city. These industrial facilities consume and discharge large amounts of water. Therefore, thermal power plants are often located near water bodies that need to assess their ecological state and predict the consequences of the anthropogenic impact. The purpose of the work was to assess the ecological state of the floodplain Lake Ivanovskoye (Russia, Kirov region), which receives the wastewater from a thermal power plant, using a triadic approach and comparing the results of chemical analysis, bioassay and bioindication. It was shown that the main pollutant in the lake and the canal connecting Lake Ivanovskoye with the nearest river Vyatka is ammonium ion (up to 3.2 mg/dm3). In addition, the Russian standards for biological oxygen consumption are exceeded (analysis period is 20 days). The tests for the toxicity of wastewater showed inhibition of Paramecium caudatum and Escherichia coli reactions. In contrast, natural surface water from Lake Ivanovskoye stimulated the test functions of Scenedesmus quadricauda, P. caudatum, and E. coli. The death of Daphnia magna in the samples were not recorded. The totality of the facts pointed to the organic nature of pollution, leading to the anthropogenic eutrophication of the lake. The hypothesis was tested by using the bioindication method and by analyzing the totality of species of coastal aquatic plants. It was shown that the reservoir belongs to the mesotrophic type with a moderate degree of pollution, which decreases with distance from the wastewater discharge points. The increase in the trophicity of the reservoir is facilitated by thermal pollution (up to +15℃) and an alkaline pH of wastewater (up to 9.2). Thus, the joint analysis of the results of chemical analysis, bioassay and bioindication made it possible to carry out an objective assessment of the ecological state of the reservoir and identify the reasons for the revealed facts.
Źródło:
Journal of Ecological Engineering; 2021, 22, 2; 155-160
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intraspecific Sensitivity to Toxicants – a Methodological Problem of Bioassay: Review
Autorzy:
Olkova, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1955380.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
bioassay
environmental pollution
intraspecific sensitivity
genetic factors
genetically unconditioned factors
Opis:
The article discusses the scientific studies that reveal the mechanisms of intraspecific differences in responses of organisms to chemical exposure. The factors of individual sensitivity to toxicants are represented by genetic differences between individuals of the same species and genetically unconditioned factors. The genetic factors are mutations, including under-researched mitochondrial DNA mutations and genomic drift, genetic polymorphism, and gender-related differences. Age, conditions of keeping and state of organisms, seasonal variations of body functions, and biotic interactions are considered as the factors that are not related to the genetic apparatus. The article considers a number of works, in which the effects of the combined action of external factors on sensitivity of organisms in model groups are studied.
Źródło:
Journal of Ecological Engineering; 2021, 22, 7; 113-122
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Comparison of Sensitivity of Autotrophic and Heterotrophic Microorganisms to the Pollution of Natural Water with Rare Earth Elements (Lanthanum and Cerium)
Autorzy:
Lozhkina, Roza A.
Olkova, Anna S.
Koval, Ekaterina V.
Tomilina, Irina I.
Sysolyatina, Maria A.
Powiązania:
https://bibliotekanauki.pl/articles/2086379.pdf
Data publikacji:
2022
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
rare earth elements
lanthanum
cerium
bioassay
Nostoc linckia
Chlorella vulgaris
Paramecium caudatum
Escherichia coli
Opis:
The purpose of the work was to compare the sensitivity of autotrophic and heterotrophic organisms used in bioassay to lanthanum and cerium under the conditions of a model experiment with aqueous media. Using bioassay methods, the pre-lethal effects of La and Ce in heterotrophic Paramecium caudatum and Escherichia coli, as well as autotrophic Chlorella vulgaris and Nostoc linckia were determined. Model solutions of La2 (SO4)3∙8H2O and Ce2 (SO4)3∙8H2O were tested in the concentration range of 0.1–200 mg/l. As a result, it was shown that heterotrophic organisms are more sensitive to water pollution with La and Ce than autotrophic ones. According to the totality of experiments, cerium turned out to be more toxic than lanthanum. When planning the environmental studies of wastewater or reservoirs polluted with REE, it is recommended to focus on comparative sensitivity of bioassay methods, taking into account the test-functions used: bioassay for chemotaxis of P. caudatum > bioassay for changes in bioluminescence of E. coli (strain M-17) > bioassay for the content of chlorophyll а and intensity of lipid peroxidation in N. linckia > bioassay on the increase in the number of Cl. vulgaris.
Źródło:
Journal of Ecological Engineering; 2022, 23, 4; 58--63
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-6 z 6

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