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    Wyświetlanie 1-10 z 10
    Tytuł:
    Solubilization and one-step purification of mannosylphosphodolichol synthase from Trichoderma reesei
    Autorzy:
    Kruszewska, Joanna
    Perlińska-Lenart, Urszula
    Palamarczyk, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1045160.pdf
    Data publikacji:
    1996
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1996, 43, 2; 397-401
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    10-Undecynoic acid, an inhibitor of cytochrome P450 4A1, inhibits ethanolamine-specific phospholipid base exchange reaction in rat liver microsomes
    Autorzy:
    Lenart, Jacek
    Pikuła, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1044558.pdf
    Data publikacji:
    1999
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1999, 46, 1; 203-210
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Evidence for the involvement of a 66 kDa membrane protein in the synthesis of sterolglucoside in Saccharomyces cerevisiae
    Autorzy:
    Lenart, Urszula
    Haplova, Jana
    Magdolen, Peter
    Farkas, Vladimir
    Palamarczyk, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1045263.pdf
    Data publikacji:
    1995
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1995, 42, 2; 269-274
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Alterations in protein secretion caused by metabolic engineering of glycosylation pathways in fungi
    Autorzy:
    Kruszewska, Joanna
    Perlińska-Lenart, Urszula
    Górka-Nieć, Wioletta
    Orłowski, Jacek
    Zembek, Patrycja
    Palamarczyk, Grażyna
    Powiązania:
    https://bibliotekanauki.pl/articles/1040697.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycosylation
    protein secretion
    Trichoderma
    Opis:
    Due to its natural properties, Trichoderma reesei is commonly used in industry-scale production of secretory proteins. Since almost all secreted proteins are O-glycosylated, modulation of the activity of enzymes of the O-glycosylation pathway are likely to affect protein production and secretion or change the glycosylation pattern of the secreted proteins, altering their stability and biological activity. Understanding how the activation of different components of the O-glycosylation pathway influences the glycosylation pattern of proteins and their production and secretion could help in elucidating the mechanism of the regulation of these processes and should facilitate creation of engineered microorganisms producing high amounts of useful proteins. In this review we focus on data concerning Trichoderma, but also present some background information allowing comparison with other fungal species.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 3; 447-456
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Protein production and secretion in an Aspergillus nidulans mutant impaired in glycosylation.
    Autorzy:
    Perlińska-Lenart, Urszula
    Kurzątkowski, Wiesław
    Janas, Piotr
    Kopińska, Agnieszka
    Palamarczyk, Grażyna
    Kruszewska, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1041477.pdf
    Data publikacji:
    2005
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    glycosylation
    protein secretion
    DPM1 gene
    Aspergillus
    dolichylphosphate mannose synthase
    Opis:
    O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion.
    Źródło:
    Acta Biochimica Polonica; 2005, 52, 1; 195-205
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The induction of cytochrome P450 isoform, CYP4A1, by clofibrate coincides with activation of ethanolamine-specific phospholipid base exchange reaction in rat liver microsomes
    Autorzy:
    Lenart, Jacek
    Komańska, Izabela
    Jasińska, Renata
    Pikuła, Sławomir
    Powiązania:
    https://bibliotekanauki.pl/articles/1044862.pdf
    Data publikacji:
    1998
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Źródło:
    Acta Biochimica Polonica; 1998, 45, 1; 119-126
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimization of a retroviral vector for transduction of human CD34 positive cells
    Autorzy:
    Szyda, Anna
    Paprocka, Maria
    Krawczenko, Agnieszka
    Lenart, Katarzyna
    Heimrath, Jerzy
    Grabarczyk, Piotr
    Mackiewicz, Andrzej
    Duś, Danuta
    Powiązania:
    https://bibliotekanauki.pl/articles/1041181.pdf
    Data publikacji:
    2006
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    transduction optimization
    retroviral vector
    CD34+ cells
    Opis:
    Human stem and progenitor cells have recently become objects of intensive studies as an important target for gene therapy and regenerative medicine. Retroviral vectors are among the most effective tools for genetic modification of these cells. However, their transduction efficiency strongly depends on the choice of the ex vivo transduction system. The aim of this study was to elaborate a system for retroviral vector transduction of human CD34 positive cells isolated from cord blood. The retroviral vector pMINV EGFP was chosen for transduction of two human erythroblastoid cell lines: KG-1a (CD34 positive) and K562 (CD34 negative). For vector construction, three promoters and two retroviral vector packaging cell lines were used. To optimize the physicochemical conditions of the transduction process, different temperatures of supernatant harvesting, the influence of centrifugation and the presence of transduction enhancing agents were tested. The conditions elaborated with KG-1a cells were further applied for transduction of CD34 positive cells isolated from cord blood. The optimal efficiency of transduction of CD34 positive cells with pMINV EGFP retroviral vector (26% of EGFP positive cells), was obtained using infective vector with LTR retroviral promoter, produced by TE FLY GA MINV EGFP packaging cell line. The transduction was performed in the presence of serum, at 37°C, with co-centrifugation of cells with viral supernatants and the use of transduction enhancing agents. This study confirmed that for gene transfer into CD34 positive cells, the detailed optimization of each element of the transduction process is of great importance.
    Źródło:
    Acta Biochimica Polonica; 2006, 53, 4; 815-823
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Disruption of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition
    Autorzy:
    Górka-Nieć, Wioletta
    Pniewski, Michał
    Kania, Anna
    Perlińska-Lenart, Urszula
    Palamarczyk, Grażyna
    Kruszewska, Joanna
    Powiązania:
    https://bibliotekanauki.pl/articles/1040737.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    Trichoderma reesei
    pmt1 gene disruption
    protein glycosylation
    cell wall composition
    Opis:
    In fungi transfer of the first mannosyl residue to proteins during their O-glycosylation is catalyzed by protein O-mannosyltransferases encoded by pmt genes. Disruption of the pmt1 gene in Trichoderma caused a significant decrease in the total activity of protein O-mannosyltransferases. Moreover, disruption of the pmt1 gene also led to osmotic sensitivity of the strain, indicating an essential role of the PMTI protein activity for cell wall synthesis. At the same time, the strain was defective in septa formation, producing only half the number of septa per unit length of hypha compared with the wild type. Disruption of the pmt1 gene decreased protein secretion but had no effect on glycosylation of secreted proteins, which suggests that PMTI protein O-mannosyltranferase does not take part in glycosylation of these proteins.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 2; 251-259
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of specific lytic and latent transcripts can help to predict the status of Epstein-Barr virus infection in transplant recipients with high virus load
    Autorzy:
    Zawilinska, Barbara
    Kosinska, Anna
    Lenart, Marzena
    Kopec, Jolanta
    Piatkowska-Jakubas, Beata
    Skotnicki, Aleksander
    Kosz-Vnenchak, Magdalena
    Powiązania:
    https://bibliotekanauki.pl/articles/1040672.pdf
    Data publikacji:
    2008
    Wydawca:
    Polskie Towarzystwo Biochemiczne
    Tematy:
    bone marrow transplantation
    Epstein-Barr virus
    latency
    EBV-lymphoproliferative disorder
    productive infection
    Opis:
    Epstein-Barr virus (EBV), a member of the family Herpesviridae, is widely spread in the human population and has the ability to establish lifelong latent infection. In immunocompetent individuals the virus reactivation is usually harmless and unnoticeable. In immunocompromised patients productive infection or type III latency may lead to EBV-associated post-transplant lymphoproliferative disorder (PTLD). The aim of our research was to investigate the utility of PCR-based methods in the diagnosis and monitoring of EBV infections in bone marrow transplant recipients. Thirty-eight peripheral blood leukocyte samples obtained from 16 patients were analysed, in which EBV DNA was confirmed by PCR. We used semi-quantitative PCR to estimate the viral load and reverse-transcription PCR (RT-PCR) to differentiate between latent and productive EBV infection. In 14 patients we confirmed productive viral infection. We observed a correlation between higher number of EBV genome copies and the presence of transcripts specific for type III latency as well as clinical symptoms.
    Źródło:
    Acta Biochimica Polonica; 2008, 55, 4; 693-699
    0001-527X
    Pojawia się w:
    Acta Biochimica Polonica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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