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    Wyświetlanie 1-4 z 4
    Tytuł:
    Micropropagation of Calycanthus fertilis
    Autorzy:
    Kulpa, D.
    Kubus, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/41353.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    in vitro culture
    micropropagation
    Calycanthus fertilis
    multiplication
    plant growth regulator
    Opis:
    Calycanthus fertilis Walt. is a shrub belonging to the family Calycanthaceae, has great potential as ornamental. In the literature there are no reports on methodpropagation of this shrub in in vitro cultures. Therefore, the aim of this study was a development of the method micropropagation of Calycanthus fertilis Walter. Shoot explants of the size 1 cm, with an apex or node with lateral meristems were placed on the media with mineral composition according to MS and WPM supplemented with BAP (from 0.5 to 2.0 mg·dm–3) and TDZ (from 0.1 to 0.5 mg·dm–3). BAP turnedout to significantly increase initiation frequency whereas TDZ inhibitedthe formation of adventitious shoots andcausedexplant death. The highest percentage of initiated explants were found in shoot fragments placed on WPM medium supplemented with 1.0 mg·dm–3 BAP. Primary explants which initiatedgrowth were transferredon the proliferation media containing WPM macroand microelement, with the addition of different cytokinin: BAP (from 0.5 to 2.5 mg·dm–3), KIN (1.0 to 5.0 mg·dm–3) or TDZ (from 0.1 to 0.5 mg·dm–3). Calycanthus multiplication in vitro shouldbe conductedon WPM media with 1.0 mg·dm–3 BAP. Proliferatedshoots were placedon the WPM rooting medium supplemented with auxins: IBA, IAA or NAA at the concentration from 0.1 to 2.0 mg·dm–3. Maximum rooting was obtained on WPM medium supplemented with 0.5 mg·dm–3 IBA. To sum up, it shouldbe statedthat an efficient method of micropropagation of Calycanthus fertilis Walt. has been developed.
    Źródło:
    Dendrobiology; 2010, 64
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Influence of growth retardants on growth and flower bud formation in rhododendron and azalea
    Autorzy:
    Marosz, A
    Matysiak, B.
    Powiązania:
    https://bibliotekanauki.pl/articles/41091.pdf
    Data publikacji:
    2005
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    azalea
    rhododendron
    flower bud formation
    plant growth
    growth retardant
    growth inhibition
    growth regulator
    plant cultivar
    Opis:
    Plants of ‘Catawbiense Boursault’ and ‘Eskimo’ rhododendrons or ‘Cannon’s Double’ and ‘Kilian’ azalea grown in 4 l containers were treated twice with chlormequat (2000, 4000 and 8000 mg · dm-3), trinexapac-ethyl (50, 100, 200, 400 mg · dm-3), daminozide (2500, 5000, 7500 mg · dm-3), proxeadione calcium (75, 150, 300 mg · dm-1) and once with paclobutrazol (50, 100, 200, 400 mg · dm-3). Shoot length of the subsequent growth flush following the treatments decreased with increasing rates of the growth retardants. The number of flower buds per plant increased with increasing rates of pacloburazol, chlormequat and daminozide. Prohexadione calcium was less effective in flower bud initiation and the worst results were obtained with trinexapac-ethyl.
    Źródło:
    Dendrobiology; 2005, 54; 35-40
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The effect of culture medium formulation on Pinus nigra somatic embryogenesis
    Autorzy:
    Salaj, T.
    Fraterova, L.
    Carach, M.
    Salaj, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/41577.pdf
    Data publikacji:
    2014
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    culture medium
    formulation
    Pinus nigra
    European black pine
    histology
    conifer
    plant growth regulator
    somatic embryo
    somatic embryogenesis
    Opis:
    The potential of immature zygotic embryos to produce embryogenic tissue was tested using culture media differed in inorganic salt composition as well as plant growth regulator content. Explants cultured on DCR as well as MLV media gave the highest initiation percentages with maximum values of 8.8% (MLV) to 10.4% (DCR). Plant growth regulators content (standard or reduced) had no profound effect on embryogenic tissue initiation. Somatic embryo maturation as well as germination was dependent on the cell line and salt composition of the medium. Plantlet regeneration occurred in three cell lines out of five tested. During the maturation process profound changes occurred in the internal organisation of somatic embryos such differentiation of root meristem in precotyledonary somatic embryos and formation of provascular strands in cotyledonary somatic embryos.
    Źródło:
    Dendrobiology; 2014, 71
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Micropropagation of Stryphnolobium japonicum
    Autorzy:
    Kobus, M
    Kulpa, D.
    Powiązania:
    https://bibliotekanauki.pl/articles/41239.pdf
    Data publikacji:
    2009
    Wydawca:
    Polska Akademia Nauk. Instytut Dendrologii PAN
    Tematy:
    Styphnolobium japonicum zob.Sophora japonica
    Sophora japonica
    Japanese pagoda tree
    pagoda tree zob.Japanese pagoda tree
    scholar zob.japanese pagoda tree
    Chinese scholar zob.Japanese pagoda tree
    micropropagation
    vegetation
    development
    in vitro culture
    old tree
    growth regulator
    primary regeneration
    shoot multiplication
    rooting
    Opis:
    Observations of Japanese pagoda trees indicate that they undergo a full cycle of vegetative and generative development without self-renovation. The aim of this study was to obtain a successive media protocol propagation of Japanese pagoda tree by in vitro cultures. The effects of growth regulators were studied with reference to primary regeneration, shoot multiplication and rooting. As explants source were used the part of shoot of 90-year-oldtree. Explants were placed on MS (Murashige and Skoog 1962) basal medium with the addition of 6-benzylaminopurine – BAP (1.0–2.0 mg dm-3), thidiazuron – TDZ (0.1–0.3 mg dm-3) and indole-3-acetic acid – IAA (0.5–1.0 mg dm-3). BAP was the growth regulator which significantly increased shoot regeneration on initial explants. TDZ in turn, inhibited the formation of adventitious shoots and caused the explants which had been placed on the medium to die. The multiplication of the Japanese pagoda tree by in vitro cultures should be conducted on MS media with the addition of 0.5 mg dm-3 BAP, and they should be rooted on media with the addition of 0.3 mg dm-3 indole-3-butyric acid – IBA. It seems, that devising an efficient method of Japanese pagoda tree micropropagation is realistic.
    Źródło:
    Dendrobiology; 2009, 61; 23-26
    1641-1307
    Pojawia się w:
    Dendrobiology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-4 z 4

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