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Wyszukujesz frazę "fluorescence" wg kryterium: Temat


Tytuł:
Fluorescence optical analysis method for assessing homogeneity of granular mixtures
Autorzy:
Matuszek, Dominika B.
Królczyk, Jolanta B.
Powiązania:
https://bibliotekanauki.pl/articles/1848991.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fluorescence
ultraviolet radiation
homogeneity
multicomponent granular mixtures
tracer
Opis:
This paper presents a method of optical fluorescence analysis for the evaluation of homogeneity of multicomponent grain mixtures. This method is based on the evaluation of the content of fluorescent marker. Maize with two degrees of fineness d1 = 1.25 mm and d2 = 2.00 mm was used as a tracer. Maize was covered with Rhodamine B, which emits red light under the influence of ultraviolet radiation. The tracer was introduced into the mixture before the mixing process began. Nine multicomponent grain mixtures were used. The proportion of fluorescent maize was evaluated on the basis of computer image analysis. Additionally, the fraction of the tracer was evaluated using a control method (validation of the accuracy of the proposed method). The results indicate that the degree of the tracer’s fineness influences the results obtained. The use of fluorescent maize with particle size d2 = 2.00 mm allowed to obtain results which differed less from the control method. The average size of the difference in results ranged from 0.20-0.38 for the 2.00 mm tracer and 0.38-1.34 for the 1.25 mm tracer.
Źródło:
Metrology and Measurement Systems; 2021, 28, 1; 41-54
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
1;25 centric fusion recognition in the cattle chromosome by fluorescence in situ hybridization
Autorzy:
Ladon, D
Solinas-Toldo, S
Fries, R
Stranzinger, G F
Powiązania:
https://bibliotekanauki.pl/articles/2047680.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cattle chromosome
hybridization
fluorescence
cattle
centric fusion
Opis:
Fluorescence in situ hybridization (FISH) experiments with specific probes for chromosome 29 and 25 were carried out on a Brown Swiss bull, previously diagnosed as a carrier of 1;29 centric fusion. The hybridization of the chromosome 29-specific probe (BMC 4216 - already located on 29q13), produced signals on two small acrocentrics, but not on the translocated chromosome. The signals appeared on the translocated chromosome and on a single chromosome 25 after hybridization of the chromosome-specific probe (BMC 3224 - previously located on 25q24). According to the actual nomenclature, the analysed aberration is a robertsonian translocation involving chromosomes 1 and 25.
Źródło:
Journal of Applied Genetics; 1996, 37, 1; 101-104
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Light-triggered protochlorophyllide to chlorophyllide reduction – new insights into enzyme-substrate interactions
Autorzy:
Gabruk, M.
Kruk, J.
Mysliwa-Kurdziel, B.
Powiązania:
https://bibliotekanauki.pl/articles/79867.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biosynthesis
chlorophyll
photosynthetic pigment
protochlorophyllide
chlorophyllide
enzyme substrate interaction
fluorescence
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning
Autorzy:
Walczak, R.
Powiązania:
https://bibliotekanauki.pl/articles/202285.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fluorescence
microfluidics
lab-on-a-chip
point-of-care
Opis:
The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from “large-scale” analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor – based detection instrumentation co-working with a “clever” software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author’s works.
Źródło:
Bulletin of the Polish Academy of Sciences. Technical Sciences; 2011, 59, 2; 157-165
0239-7528
Pojawia się w:
Bulletin of the Polish Academy of Sciences. Technical Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Unraveling protein-protein interactions using fluorescence imaging techniques
Autorzy:
Michalak, M.
Wojtaszek, P.
Powiązania:
https://bibliotekanauki.pl/articles/79933.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
protein-protein interaction
imaging technique
fluorescence
physiological process
living cell
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Protochlorophyllide photoreduction in angiosperms: focus on protochlorophyllide – surroundings interaction in model system
Autorzy:
Mysliwa-Kurdziel, B.
Gabruk, M.
Kruk, J.
Strzalka, K.
Powiązania:
https://bibliotekanauki.pl/articles/79978.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
protochlorophyllide
angiosperm
chlorophyll
photosynthetic reaction
fluorescence intensity
imidazole
model system
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 3
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A low ratio of red/far-red in the light spectrum accelerates senescence in nest leaves of Platycerium bifurcatum
Autorzy:
Oliwa, Jakub
Kornas, Andrzej
Skoczowski, Andrzej
Powiązania:
https://bibliotekanauki.pl/articles/952109.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
hlorophyll a fluorescence
fern
nest leaves
photomorphogenesis
r/fr ratio
reflectance
Opis:
The fern Platycerium bifurcatum is a valuable component of the flora of tropical forests, where degradation of local ecosystems and changes in lighting conditions occur due to the increasing anthropogenic pressure. In ferns, phytochrome mechanism responsible for the response to changes in the value of R/FR differs from the mechanism observed in spermatophytes. This study analyzed the course of ontogenesis of nest leaves in P. bifurcatum at two values of the R/FR ratio, corresponding to shadow conditions (low R/FR) and intense insolation (high R/FR). The work used only non-destructive research analysis, such as measurements of reflectance of radiation from the leaves, their blue-green and red fluorescence, and the chlorophyll a fluorescence kinetics. This allowed tracing the development and aging processes in the same leaves. Nest leaves are characterized by short, intense growth and rapid senescence. The study identified four stages of development of the studied leaves related to morphological and anatomical structure and changing photochemical efficiency of PSII. Under the high R/FR ratio, the rate of ontogenesis of the leaf lamina was much slower than under the low R/FR value. As shown, the rapid aging of the leaves was correlated with faster decline of the chlorophyll content. It was shown that leaf senescence was accompanied by accumulation of polyphenols, anthocyanins and carotenoids on the basis of reflectance and fluorescence measurements in the blue-green range.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2017, 59, 2
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Indentification of three different chromosomal additions by chromosome painting using fluorescence in situ hybridization [FISH] technique
Autorzy:
Bocian, E
Stankiewicz, P
Stanczak, H
Obersztyn, E
Mazurczak, T
Powiązania:
https://bibliotekanauki.pl/articles/2047283.pdf
Data publikacji:
1996
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosomal addition
fluorescence in situ hybridization method
hybridization
chromosome painting
cytogenetics
Opis:
Fluorescence in situ hybridization (FISH) is a very useful method for assessing chromosome rearrangements. When neither banding pattern nor clinical symptoms are sufficient to determine the origin of additional chromosomal fragment, FISH with multiple chromosome-specific libraries (chromosome painting), allows to solve this diagnostic problem rapidly. Three chromosomal additions, 7q+, 13p+ and 22q+, found in routine cytogenetic studies performed in children with phenotypic abnormalities were analysed using FISH. This technique documented the origin of the extra material to be derived from chromosome 16[der(7)t(7; 16)(q36.3;p 13.11)], 18[der(13)t(13; 18)(p12;q 12.2)] and 22[dup(22)(q11.2q13.1)], respectively. In two cases the abnormality arose de novo, while in the third case the product of translocation t(13;18) was maternal by origin. It was present in 30% of mother's lymphocytes, and in 70% of them a balanced Robertsonian translocation t(13q;15q) was found. In the presented cases the chromosome analysis with both traditional banding and chromosome painting techniques, allowed to establish final clinical diagnosis.
Źródło:
Journal of Applied Genetics; 1996, 37, 2; 197-204
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Photosynthetic activity in three vascular species of Spitsbergen vegetation during summer season in response to microclimate
Autorzy:
Barták, Miloš
Váczi Peter
Hájek, Josef
Powiązania:
https://bibliotekanauki.pl/articles/2051428.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Arctic
Svalbard
vascular plants
plant ecophysiology
net photosynthesis
chlorophyll fluorescence
Źródło:
Polish Polar Research; 2012, 4; 443-462
0138-0338
2081-8262
Pojawia się w:
Polish Polar Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Simultaneous velocity measurement of phases in a liquid-gas system
Autorzy:
Musoski, R.
Stelmach, J.
Powiązania:
https://bibliotekanauki.pl/articles/185238.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
fluorescence
self-aspirating impeller
phase velocity
PIV
fluorescencja
samozasysający wirnik
prędkość fazowa
Opis:
Results of velocity measurements of liquid and gas bubbles in a tank with a self-aspirating disk impeller are analysed. Studies were carried out using a fluorescent dye tracer in the measuring system with two cameras (simultaneous phase velocity measurement) and with one camera (sequential measurement of phase velocity). Based on a comparative analysis of the acquired data it was found that when differences in the phase velocities were small the simultaneous velocity measurement gave good results. However, sequential measurement gives greater possibilities for setting the measuring system and if the analysis of instantaneous velocities is not necessary, it seems to be a better solution.
Źródło:
Chemical and Process Engineering; 2017, 38, 4; 577-585
0208-6425
2300-1925
Pojawia się w:
Chemical and Process Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Estimation of mating-compatibility of the wild service tree [Sorbus torminalis [L.] Crantz] by observing pollen tube germination
Autorzy:
Wojciechowski, A
Bednorz, L.
Powiązania:
https://bibliotekanauki.pl/articles/2042041.pdf
Data publikacji:
2000
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
self-incompatibility
mating-compatibility
pollen tube
fluorescence
tree
Sorbus torminalis
germination
Źródło:
Journal of Applied Genetics; 2000, 41, 4; 253-258
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
FISH mapping of 18S-5.8S-26S rRNA genes and fluorochrome banding in the triploid viviparous onion Allium x cornutum Clementi ex Visiani, 1842
Autorzy:
Lepen, I.
Puizina, J.
Powiązania:
https://bibliotekanauki.pl/articles/19898.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
rRNA gene
fluorochrome banding
triploid
Allium cepa var.viviparum
fluorescence in situ hybridization
FISH zob.fluorescence in situ hybridisation
mapping
onion
Allium
viviparous onion
Allium cepa var.proliferum
Opis:
Triploid viviparous onions [Allium × cornutum Clementi ex Visiani 1842, syn Allium cepa L. var. viviparum Metzg. (ALEF.), auct.] (2n = 3x = 24), are known in some countries only as rare relict crops. In other parts of the world they are still traditionally or even commercially cultivated. In previous cytogenetic studies of the Croatian triploid viviparous onion Ljutika, Giemsa C-banding, chromosome pairing analysis during meiosis, and genomic hybridization in situ indicated a complex hybrid with highly heterozygous karyotype structure, with possible triparental genome organization. This study continues an analysis of the karyotype structure of Ljutika. Staining with fluorochromes CMA3 (Chromomycin A3) and DAPI (4,6-diamidino-2-phenylindole) confirmed previous results from Giemsa C-banding and revealed GC-rich heterochromatic regions associated mainly with chromosome ends and nucleolus organizing regions (NORs), and only a few interstitial bands. FISH mapping of the ribosomal 18S-5.8S-26S genes revealed two major rDNA signals on the short arms of two subtelocentric satellite chromosomes in almost all metaphase plates of Ljutika. The largest subtelocentric chromosome lacked rDNA signals. A significantly smaller rDNA signal was occasionally located on one small submetacentric chromosome. These results are in agreement with previously published results from identification of NORs by silverstaining technique, which confirmed a maximum three nucleoli in interphase nuclei. We discuss the molecular mechanisms underlying rearrangements and activity of ribosomal genes in the triploid karyotype.
Źródło:
Acta Biologica Cracoviensia. Series Botanica; 2011, 53, 1
0001-5296
Pojawia się w:
Acta Biologica Cracoviensia. Series Botanica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular versus classical cytogenetics - evaluation of 20 Prader-Willi syndrome patients
Autorzy:
Stankiewicz, P
Lebiocka, J
Szpecht-Potocka, A
Bocian, E
Stanczak, H
Obersztyn, E
Mazurczak, T
Powiązania:
https://bibliotekanauki.pl/articles/2047150.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
chromosomal deletion
in situ
patient
hybridization
fluorescence
cytogenetics
chromosome region
Prader-Willi syndrome
Opis:
Prader-Willi syndrome (PWS) is a developmental disorder caused by a deficiency of paternal contribution of the chromosome region 15q11.2-q13 arising from differently sized deletions, maternal disomy, or rarely imprinting mutations. We have analyzed 20 PWS patients using combined cytogenetic high resolution technique (HRT), fluorescence in situ hybridization (FISH) and molecular studies to identify parental origin (uniparental disomy) or molecular defect (deletion) of the Prader-Willi region. Lack of a paternal copy of 15q11.2-q13 resulting from its deletion was found in 16 patients. Using high resolution GTG banding on prometaphase chromosomes, deletion in the 15q11.2-q13 region was detected in only 8 patients. Application of FISH with different sets of PWS specific unique sequence probes (D15S11, SNRPN, D15S10, GABRß3) revealed microdeletions in 12 patients. In 12 out of 20 cases FISH confirmed HRT studies, while in 8 cases inconsistent results were obtained. No discrepancies between results of FISH and molecular studies were found, although the latter had a higher sensitivity. We conclude that FISH appears to be a rapid and reliable method of microdeletion identification and should be performed as a method of choice in cytogenetic diagnosis of Prader-Willi syndrome.
Źródło:
Journal of Applied Genetics; 1997, 38, 2; 217-226
1234-1983
Pojawia się w:
Journal of Applied Genetics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
De-etiolation of cucumber cotyledons under different light sources: effect of nitric oxide
Autorzy:
Bargareche, C.
Rebula, D.
Moysset, L.
Simon, E.
Powiązania:
https://bibliotekanauki.pl/articles/80365.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
conference
Cucumis sativus
cucumber
cotyledon
light source
nitric oxide
photosynthetic pigment content
chlorophyll fluorescence
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro cultures of animal and human cells
Autorzy:
Slomski, R.
Nowak, A.
Hryhorowicz, M.
Powiązania:
https://bibliotekanauki.pl/articles/951215.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
in vitro culture
tissue culture
animal cell
human cell
homeostasis
fluorescence in situ hybridization
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2015, 96, 1
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł

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