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Wyszukujesz frazę "Wu, L." wg kryterium: Autor

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    Wyświetlanie 1-15 z 15
    Tytuł:
    The ascorbate metabolism as a target for the breeding of stress resistant rice
    Autorzy:
    Frei, M.
    Holler, S.
    Ueda, Y.
    Wu, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/80002.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    conference
    ascorbate
    abiotic stress
    plant tissue
    oxidative stress
    rice
    stress resistance
    zinc deficiency
    tropospheric ozone
    iron toxicity
    Źródło:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2013, 94, 2
    0860-7796
    Pojawia się w:
    BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of porcine epidemic diarrhea virus infection on tight junction protein gene expression and morphology of the intestinal mucosa in pigs
    Autorzy:
    Zong, Q.F.
    Huang, Y.J.
    Wu, L.S.
    Wu, Z.C.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087524.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pig
    tight junction protein
    PEDV
    diarrhea
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 345-353
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A fault diagnosis method for substation grounding grid based on the square-wave frequency domain model
    Autorzy:
    Zhang, P.-H.
    He, J.-J.
    Zhang, D.-D.
    Wu, L.-M.
    Powiązania:
    https://bibliotekanauki.pl/articles/221635.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    square-wave
    frequency characteristics
    grounding grid
    fault diagnosis
    Opis:
    Current methods of fault diagnosis for the grounding grid using DC or AC are limited in accuracy and cannot be used to identify the locations of the faults. In this study, a new method of fault diagnosis for substation grounding grids is proposed using a square-wave. A frequency model of the grounding system is constructed by analyzing the frequency characteristics of the soil and the grounding conductors into which two different frequency square-wave sources are injected. By analyzing and comparing the corresponding information of the surface potentials of the output signals, the faults of the grounding grid can be diagnosed and located. Our method is verified by software simulation, scale model experiments and field experiments.
    Źródło:
    Metrology and Measurement Systems; 2012, 19, 1; 63-71
    0860-8229
    Pojawia się w:
    Metrology and Measurement Systems
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    An EL-model based passivity control of four-phase interleaved PFC
    Autorzy:
    Hua-Wu, L.
    Hong-Xing, M.
    Jian-Feng, J.
    Xi-Jun, Y.
    Xing-Hua, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/140472.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    four-phase interleaved PFC
    passivity based control
    damping injection
    current sparing
    Opis:
    With the continuous increase of output power ratings, multi-phase (multichannel) interleaved power factor corrector (IPFC) is gradually employed in domestic and commercial inverter air-conditioners. IPFC can solve several main problems, such as power rating increase, power device selection, input current ripple reduction as well as inductor on-board mounting. But for a multi-phase IPFC, the key problem is that it should show rapid dynamic responds and good current sharing capability, so in this paper the aim is to improve the dynamic performance and current sharing capability by means of passivity control theory. Considering the power circuit topology of a four-phase IPFC, an EL (Euler-Lagrange) mathematical model is established when the IPFC operates in continuous conduction mode (CCM). Then the passivity of the four-phase IPFC is proved, and the passivity-based controller using the state variables feedback and damping injection method is designed. The proposed control scheme, which is easy to control and needs no proportion integral controller, has strong robustness on disturbance from singlephase AC input voltage, the load as well as the parameters of the employed devices. Even in wide-range load condition, the mains current has a fast dynamic response and the average output voltage almost keep unchanged. As a result, the main functions of the four-phase IPFC are implemented including nearly unitary power factor and constant DC output voltage. Meanwhile, the four-phase IPFC acquires an excellent current sparing effect after using passivity-based controller. The above analysis has been proved with simulated results by means of MATLAB/SIMULINK and experimental results, showing that the passivity-based IPFC controller has superior performances and feasibility.
    Źródło:
    Archives of Electrical Engineering; 2013, 62, 4; 613-628
    1427-4221
    2300-2506
    Pojawia się w:
    Archives of Electrical Engineering
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effect of bamboo vinegar powder on the expression of the immune-related genes MyD88 and CD14 in weaning piglets
    Autorzy:
    Qin, W.Y.
    Gan, L.N.
    Dong, L.
    Yu, L.H.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087674.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    bamboo vinegar powder
    pig
    antibiotics
    MyD88 gene
    CD14 gene
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 525-532
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Effects of porcine epidemic diarrhea virus infection on Toll-like receptor expression and cytokine levels in porcine intestinal epithelial cells
    Autorzy:
    Wang, F.
    Wang, S.Q.
    Wang, H.F.
    Wu, Z.C.
    Bao, W.B.
    Wu, S.L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087351.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    pig
    porcine epidemic diarrhea virus
    TLRs
    innate immunity
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 1; 119-126
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    LBP gene methylation involved in mRNA expression and resistance to E. coli F18 in weaned piglets
    Autorzy:
    Gan, L.N.
    Bao, W.B.
    Wu, S.L.
    Qin, W.Y.
    Sun, L.
    Zhao, C.X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087872.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    LBP gene
    methylation analysis of the LBP region
    E.coli F18 strain
    weaned piglets
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 4; 643-650
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Differential expression of Toll - like receptor 4 signaling pathway genes in Escherichia coli F18 - resistant and - sensitive Meishan piglets
    Autorzy:
    Liu, Y.
    Gan, L.N.
    Qin, W.Y.
    Sun, S.Y.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31946.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The Toll-like receptor 4 (TLR4) signaling pathway is an important inflammatory pathways associated with the progression of numerous diseases. The aim of the present study was to investigate the relationship between TLR4 signaling and resistance to Escherichia coli F18 in locally weaned Meishan piglets. Using a real-time PCR approach, expression profiles were determined for key TLR4 signaling pathway genes TLR4, MyD88, CD14, IFN-α, IL-1β and TNF-α in the spleen, thymus, lymph nodes, duodenum and jejunum of E. coli F18-resistant and -sensitive animals. TLR4 signaling pathway genes were expressed in all the immune organs and intestinal tissues, and the expression was generally higher in the spleen and lymph nodes. TLR4 transcription was higher in the spleen of sensitive piglets (p<0.05), but there was no significant difference in TLR4 mRNA levels in other tissues. Similarly, CD14 transcription was higher in lymph nodes of sensitive animals (p<0.05) but not in other tissues. IL-1β expression was higher in the spleen and in the duodenum of resistant piglets (p<0.05, p<0.01, respectively), and there were no significant differences in other tissues. There were also no significant differences in the expression of MyD88, TNF-α and IFN-α between sensitive and resistant piglets (p>0.05). These results further confirm the involvement of the TLR4 signaling pathway in resistance to E. coli F18 in Meishan weaned piglets. The resistance appeared to be mediated via downregulation of TLR4 and CD14, and upregulation of MyD88 that may promote the release of cytokines TNF-α, IL-1β, IFN-α and other inflammatory mediators which help to fight against E. coli F18 infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Use of fluorescence quantitative polymerase chain reaction (PCR) for the detection of Escherichia coli adhesion to pig intestinal epithelial cells
    Autorzy:
    Dai, C.H.
    Gan, L.N.
    Qin, W.U.
    Zi, C.
    Zhu, G.Q.
    Wu, S.L.
    Bao, W.B.
    Powiązania:
    https://bibliotekanauki.pl/articles/31510.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    An efficient and accurate method to test Escherichia coli (E. coli) adhesion to intestinal epithelial cells will contribute to the study of bacterial pathogenesis and the function of genes that encode receptors related to adhesion. This study used the quantitative real-time polymerase chain reaction (qPCR) method. qPCR primers were designed from the PILIN gene of E. coli F18ab, F18ac, and K88ac, and the pig β-ACTIN gene. Total deoxyribonucleic acid (DNA) from E. coli and intestinal epithelial cells (IPEC-J2 cells) were used as templates for qPCR. The 2−ΔΔCt formula was used to calculate the relative number of bacteria in cultures of different areas. We found that the relative numbers of F18ab, F18ac, and K88ac that adhered to IPEC-J2 cells did not differ significantly in 6-, 12-, and 24-well culture plates. This finding indicated that there was no relationship between the relative adhesion number of E. coli and the area of cells, so the method of qPCR could accurately test the relative number of E. coli. This study provided a convenient and reliable testing method for experiments involving E. coli adhesion, and also provided innovative ideas for similar detection methods.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    High drilling methane drainage in fracturing zones formed by water injection into boreholes
    Odprowadzania metanu metodą odwiertów dokonywanych w strefie szczelin powstałych wskutek wprowadzania wody do otworów
    Autorzy:
    Wang, G.
    Huang, W.
    Sun, L.
    Wu, M.
    Zhang, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/220166.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    odwierty
    odprowadzenie metanu
    wtrysk wody
    szczelinowanie
    odkształcenia
    high drilling
    methane drainage
    water injection
    fracturing zones
    strain
    Opis:
    Methane drainage method should be used before coal mining of many modern collieries because venti lation air methane is in sufficient to keep methane level within regulation values. The technology of high drilling methane drainage (HDMD) has been used for methane drainage although its effect is not very stable due to parameter design. The height of the fracturing zones is determined mostly according to empirical formula, on-site observation and numerical simulation analysis. In this paper, a method was introduced for determining the height of the air f ract uring zones (AFZs) based on its high similarity to the characteristics of Fracturing zones and the relationship between the height of Fracturing zones and the strain of overl ying rock strata. The application of water injection in both Shuangdingshan and Dongrong collieries found that the theoretically calculated the height of the Fracturing zones was approximately equal to the measured one in field tests within a permissible error of less than 5%, proving that the method is feasible. Based on the designed drainage parameters, the u tilization of HDMD technology in the collieries mentioned above found that the methane concentrations in both tail gate and upper corner were controlled in the ranges of 0.17% to 0.32% and 0.26% to 0.84%, respectively. These results showed that the water injection verified HDMD in Fracturing zones could effectively solve the problem of metha ne overrun and also verified the accuracy and reliability of its related theory.
    Przed rozpoczęciem wydobycia węgla w wielu obecnie eksploatowanych kopalniach wskazane jest odprowadzenie metanu, ponieważ stosowane systemy wentylacji powietrza są niewystarczające aby utrzymać stężenia metanu na dopuszczalnym poziomie. Technologia odprowadzania metanu metodą odwiertów prowadzonych na różnej wysokości (HDMD) wykorzystywana jest w tym celu, choć jej wyniki nie zawsze są stabilne ze względu na konieczność doboru parametrów obliczeniowych. Wysokość strefy szczelinowania określa się zazwyczaj empirycznie, na podstawie obserwacji w terenie oraz drogą symulacji numerycznych. W pracy tej określono wysokość strefy wykonania szczelin w oparciu o podobieństwo do charakterystyk Strefy szczelinowania oraz o analizę odkształceń warstw nadkładu. Zastosowano wtrysk wody w kopalniach Shuangdingshan i Dongrong i stwierdzono, że teoretycznie obliczona wysokość stref szczelinowania była w przybliżeniu równa wysokości zmierzonej empirycznie w trakcie badań terenowych, z dopuszczalnym poziomem błędu poniżej 5%, co wskazuje na możliwość zastosowania metody. W oparciu o parametry obliczeniowe stwierdzono, że zastosowanie metody HDMD w wyżej wymienionych kopalniach spowodowało, że stężenia metanu rejestrowane w chodniku nadścianowym i górnych narożach utrzymywały się odpowiednio na poziomie 0.17%, 0.32% i 0.26%-0.84%. Wyniki te pokazują, że wtrysk wody w strefie szczelin pomaga skutecznie rozwiązać problem obecności zbyt wysokich ilości metanu, ponadto potwierdza dokładność i wiarygodność teorii na której metoda jest oparta.
    Źródło:
    Archives of Mining Sciences; 2016, 61, 1; 137-156
    0860-7001
    Pojawia się w:
    Archives of Mining Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
    Autorzy:
    Wu, X.
    Xiao, L.
    Peng, B.
    Wang, Y.
    Yang, Z.
    Yao, X.
    Hu, L.
    Lin, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/30963.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
    Autorzy:
    Bu, R.-E.
    Wang, I.-L.
    Wu, J.-H.
    Xilin, G.W.
    Chen, J.-L.
    Wang, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/30156.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 20, 2
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Polymorphic analysis of peptide binding domain of major histocompatibility complex class I in domestic ducks
    Autorzy:
    Yu, S.
    Wu, J.
    Bai, J.
    Ding, Y.
    Qiu, W.
    Zhang, L.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087551.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    domestic duck
    highly variably sites
    MHC class I
    peptide binding domain
    phylogenetic tree
    polymorphism
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 2; 415-422
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Optimal expression and purification of sapelovirus A structural protein VP1, and its immunogenicity in mice
    Autorzy:
    Zhao, T.T.
    Cui, L.
    Chen, L.
    Li, J.J.
    Liang, Q.L.
    Wu, P.J.
    Yu, X.Q.
    Zhang, Z.H.
    Hua, X.G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087686.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    sapelovirus A
    prokaryotic expression
    purification
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 573-579
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The distribution of different virulence grass carp reovirus strains in some neglected tissues
    Autorzy:
    Liang, H.R.
    Fu, X.Z.
    Li, N.Q.
    Liu, L.H.
    Lin, Q.
    Li, Y.G.
    Peng, Y.A.
    Huang, Z.B.
    Wu, S.Q.
    Powiązania:
    https://bibliotekanauki.pl/articles/30668.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-15 z 15

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