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Wyszukujesz frazę "Chmielewska, M." wg kryterium: Autor

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    Wyświetlanie 1-10 z 10
    Tytuł:
    The expression of mitochondrial, cytoplasmic and extracellular superoxide dismutase in the colonic wall of pigs suffering from swine dysenteria
    Autorzy:
    Chmielewska, M.
    Losiewicz, K.
    Podlasz, P.
    Wasowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30510.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The expression of 3 types of peroxide dismutase (SOD1, SOD2 and SOD3) was studied with Real-Time PCR in the colonic wall of domestic pig suffering from swine dysentery. The expression of enzymes was studied separately in the mucosa and the muscular membrane. It was found that in the mucosa the expression of SOD1 (cytoplasmic) did not change, while the levels of expression of mitochondrial SOD2 and extracellular SOD3 were raised in inflamed colon. More dramatic changes were seen in the muscular mebrane where expression of SOD1 rose twice, this of SOD2 rose ca. 5-fold and the expression of SOD3 rose dramatically, even 30-fold.The obtained data are contradictory to findings in other types of colonic inflammation, which were studied either in the whole colonic wall, or in mucosa alone. The results show a very strong reaction of antioxidant systems in the muscular membrane in the enteritis.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 3
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Chemical stability and sanitary properties of pelletized organo-mineral waste-derived fertilizer
    Stabilność chemiczna i właściwości sanitarne peletyzowanego nawozu organiczno-mineralnego wytworzonego z odpadów
    Autorzy:
    Kasprzycka, A.
    Lalak-Kańczugowska, J.
    Tys, J.
    Chmielewska, M.
    Pawłowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/204774.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    filamentous fungi
    digestate
    ashes from biomass combustion
    fertilizer pellets
    grzyby nitkowate
    poferment
    popioły ze spalania biomasy
    granulki nawozu
    Opis:
    Different types of organic waste can be used as fertilizers or soil improvers, but such materials are susceptible to chemical and biochemical decomposition, and their storage can entail a deterioration in their quality. Evaluation of the infl uence of water content and ambient temperature on the chemical and microbiological properties of pelletized organo-mineral fertilizer produced on the basis of digestate from biogas plant and ashes from biomass combustion was the aim of the study. The results of the short (one month’s storage in the – laboratory silo) and long-term (one year’s storage in – unheated compartment) studies showed that the quality of the fertilizer depends on the temperature, fertilizer humidity, and air accessibility. The growth in the temperature from 20 to 30°C decreased the content of total and ammonium nitrogen, while the increase in water content stimulated the development of the fungi in fertilizer stored at 20 and 30°C. The access of the ambient air resulted in the increase of moisture and volatile solids content in the fertilizer. It was stated that fertilizer should be stored without access to the atmospheric air, at the temperature below 20°C to ensure their long-term chemical stability and microbial safety.
    Celem pracy jest ocena wpływu wilgotności, temperatury i dostępu powietrza na właściwości chemiczne i mikrobiologiczne granulowanego nawozu wytworzonego na bazie pofermentu z biogazowni i popiołów ze spalania biomasy. Wyniki badań krótkoterminowych i długoterminowych wykazały, że jakość nawozu zależy od temperatury, wilgotności nawozu i dostępności powietrza. Wzrost temperatury od 20 do 30°C znacząco obniżył zawartość azotu, a wzrost wilgotności stymulował rozwój grzybów. Dostęp powietrza powodował wzrost wilgotności i zawartości substancji organicznej w nawozie. Stwierdzono, że w celu zapewnienia długoterminowej stabilności chemicznej i bezpieczeństwa mikrobiologicznego nawóz należy przechowywać bez dostępu powietrza, w temperaturze poniżej 20°C.
    Źródło:
    Archives of Environmental Protection; 2018, 44, 3; 106-113
    2083-4772
    2083-4810
    Pojawia się w:
    Archives of Environmental Protection
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The application of circulating tumor cells detecting methods in veterinary oncology
    Autorzy:
    Chmielewska, M.
    Losiewicz, K.
    Socha, P.
    Mecik-Kronenberg, T.
    Wasowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/30619.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Cancers are one of the most common diseases affecting dogs. Many of them develop spontaneously and their biology and histopathology shows many similarities to human cancers. What more, it is proved that there are much more analogies in molecular mechanisms of cancer development between these two species. Human oncology is seeking more and more efficient methods for an early disease detection which results directly in the extended life expectancy of patients affected. One of the most modern trends in the diagnosis of cancer is to detect circulating tumor cells (CTC) in the blood of patients. It is known that these cells are responsible for the formation of metastases in distant organs what results in the patient death. Moreover, it’s confirmed that CTC are already present in patients’ bloodstream in the early stages of tumor development. There is no doubt that mechanism of metastasis development in dogs is identical and thus the CTC are also present in their bloodstream. Despite the intense researches there is still no optimal method of isolating cancer cells from the blood where they occur extremely rarely. The purpose of this study is to analyze the implications of the detection methods of tumor cells in the blood in veterinary oncology.
    Źródło:
    Polish Journal of Veterinary Sciences; 2013, 16, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Stability of αB-crystallin gene expression in canine mammary gland neoplasms. Should it be considered as circulating tumor cell genetic marker?
    Autorzy:
    Chmielewska-Krzesińska, M.
    Jakimiuk, A.
    Wąsowicz, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087495.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    αB-crystallin (CRYAB)
    canine mammary gland tumor
    circulating tumor cells (CTC)
    cytokeratin 17 (CK17)
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 523-529
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Sex-dependent differences in the effect of early weaning on the chosen hormones secretion in sheep during the postnatal transition to puberty – Preliminary results
    Autorzy:
    Cieslak, .
    Chmielewska, N.
    Romanowicz, K.
    Mlynarczuk, J.
    Wankowska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087989.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    The influence of early weaning on the cortisol, follicle-stimulating hormone (FSH), luteinizing hormone (LH) and growth hormone (GH) secretion in lambs of both sexes and testosterone (T4) level in male lambs during the postnatal transition to puberty was investigated by radioimmunoassay. It was hypothesized that this influence is long-term and sexually dimorphic. Hence, the effect of weaning at 5 weeks of age in comparison with the weaning at 9 weeks of age on hormone concentrations in peripheral blood plasma of 5-, 9-, 12-, and 16-week-old lambs of both sexes was investigated. The cortisol concentrations were greater (P<0.05) in control and early weaned female lambs than in male lambs at investigated stages. Weaning at 5 weeks of age resulted in the lover (P<0.05) cortisol secretion in male lambs in contrast to the greater (P<0.05) cortisol secretion in female lambs at 16 weeks of age. Weaning at 5 weeks of age stimulated (P<0.001) the FSH secretion, but reduced (P<0.001) the LH, GH and T4 secretion in 16-week-old male lambs. In female lambs early weaning inhibited (P<0.05) the FSH secretion at 9 weeks of age, LH secretion after 9 weeks of age and GH secretion after 12 weeks of age. Thus, early weaning results in the sexually dimorphic stress reaction that is more potent and long-lasting in female in contrast to male lambs. This maternal deprivation stress contributes to the inhibition of LH and GH secretion in lambs of both sexes and T4 secretion in male lambs during the postnatal transition to puberty.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Fast Prototyping for Video Monitoring Systems with the Use of DSP Module
    Autorzy:
    Chmielewska, A.
    Weychan, R.
    Marciniak, T.
    Dąbrowski, A.
    Hartwich, M.
    Owczarczak, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/226392.pdf
    Data publikacji:
    2013
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    video processing
    CCTV system
    urban surveillance monitoring
    threat detection
    Matlab / Simulink
    Code Composer Studio
    DSP
    Opis:
    This paper presents techniques for fast prototyping of real-time hardware / software video processing systems for urban surveillance monitoring equipment. During the experimental research the evaluation module with the TMS320DM6437 signal processor programmed with the use of the Code Composer Studio and Matlab / Simulink environments has been used. Analyzed algorithms can support the work of monitoring video operators. In particular, we analyzed efficiency of implementation of the algorithms using two examples: detection of painting theft and signaling of crossing a pedestrian pass at the red light.
    Źródło:
    International Journal of Electronics and Telecommunications; 2013, 59, 4; 375-381
    2300-1933
    Pojawia się w:
    International Journal of Electronics and Telecommunications
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
    Autorzy:
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Dzieciatkowski, T.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30665.pdf
    Data publikacji:
    2012
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    xCELLigence system
    real-time analysis
    label-free analysis
    neuronal cell response
    dermal cell response
    cell response
    equine herpesvirus 1
    animal infection
    herpesvirus
    neuron
    neuronal cell
    dermal cell
    Opis:
    Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2012, 15, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
    Autorzy:
    Drebert, Z.
    Golke, A.
    Cymerys, J.
    Slonska, A.
    Chmielewska, A.
    Tucholska, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/30687.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Equine herpesvirus type 1 (EHV-1) replication in primary murine neurons culture
    Autorzy:
    Cymerys, J.
    Dzieciatkowski, T.
    Slonska, A.
    Bierla, J.
    Tucholska, A.
    Chmielewska, A.
    Golke, A.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/31974.pdf
    Data publikacji:
    2010
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    replication
    animal disease
    horse
    equine herpesvirus 1
    respiratory tract infection
    neurological disorder
    abortion
    neuron
    Opis:
    Equine herpesvirus-1 (EHV-1) infections cause significant economic losses for equine industries worldwide as a result of abortion, respiratory illness, and neurologic disease in all breeds of horses. The occurrence of abortions caused by EHV-1 has repeatedly been confirmed in Poland, but neurological manifestations of the infection have not been described yet. Also it is unknown how the infection of neurons with non-neuropathogenic strains is regulated. To further understand the virus- neuron interaction we studied two strains of EHV-1 in murine primary neuron cell cultures. Both strains were isolated from aborted fetuses: Rac-H, a reference strain isolated by Woyciechowska in 1959 (Woyciechowska 1960) and Jan-E isolated by Bańbura et al. (Bańbura et al. 2000). Upon infection of primary murine neuronal cell cultures with Jan-E or Rac-H strains, a cytopathic effect was observed, manifested by a changed morphology and disintegration of the cell monolayer. Positive results of immunofluorescence, nPCR and real-time PCR tests indicated high virus concentration in neurons, meaning that both EHV-1 strains were likely to replicate in mouse neurons in vitro without the need for adaptation. Moreover, we demonstrated that some neurons may survive (limited) virus replication during primary infection, and these neurons (eight weeks p.i.) harbour EHV-1 and were still able to transmit infection to other cells.
    Źródło:
    Polish Journal of Veterinary Sciences; 2010, 13, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line
    Autorzy:
    Cymerys, J.
    Slonska, A.
    Brzezicka, J.
    Tucholska, A.
    Chmielewska, A.
    Rola, J.
    Malik, P.
    Banbura, M.W.
    Powiązania:
    https://bibliotekanauki.pl/articles/32263.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-10 z 10

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