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    Tytuł:
    In vivo and in vitro activity of 1-aminocyclopropane-1-carboxylic acid oxidase in germinating seeds of China aster (Callistephus chinensis Nees)
    Autorzy:
    Chojnowski, M.
    Skorupinska, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/1078232.pdf
    Data publikacji:
    2020
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    1-amino-cyclopropane-1-carboxylic acid oxidase
    Callistephus chinensis
    China aster
    germinating seed
    protein determination
    oxidase activity
    Opis:
    The activity of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO; EC 1.4.3.3) in germinating seeds of Callistephus chinensis was studied. For maximum recovery of ACO activity in vitro, the presence of 10% (w/v) insoluble polyvinylpolypyrrolidone (PVPP) and 30% of glycerol in the extraction medium was necessary. The optimum pH for this activity was 7.0. Ethylene production by whole achenes or enzymatic extract increased due to increasing 1-aminocyclopropane-1-carboxylic acid (ACC) concentrations. Saturation level of ACC for in vivo ACO activity was 10-1 M and Vmax was 10.89 nL C2H4·mg protein-1·h-1. For in vitro ACO activity, the saturation level of ACC was 10-3 M and Vmax was 2.299 nL C2H4·mg protein-1·h-1. Both, in vivo and in vitro ACO activities did not follow Michaelis-Menten kinetics. The Hill coefficients (h) were estimated on the basis of non-linear estimation. Their values were 0.63 for in vivo ACO activity and 1.73 for in vitro ACO activity. The experimental data show that ACO from C. chinensis seeds is an oligomeric enzyme with at least two active sites. During seed germination, in vitro ACO activity was detectable after 12 hours of imbibition, while in vivo ACC conversion to ethylene was observed after 24 h, i.e. – after radicle protrusion. The activity of ACO in C. chinensis seeds is associated with germination sensu stricto, and might be a good marker of this process.
    Źródło:
    Journal of Horticultural Research; 2020, 28, 2; 11-20
    2300-5009
    Pojawia się w:
    Journal of Horticultural Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Wpływ krótkoterminowego przechowywania nasion osiemnastu genotypów pomidora (Solanum lycopersicum L.) na ich kiełkowanie
    Effect of short-term storage of seeds of eighteen tomato (Solanum lycopersicum L.) genotypes on their germination
    Autorzy:
    Chojnowski, M.
    Wawrzyniak, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2118938.pdf
    Data publikacji:
    2021
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    tomato
    genotypes
    seed storage
    germination
    primary dormancy
    Opis:
    The aim of the research was to determine the occurrence of primary dormancy in seeds of 18 tomato genotypes (Solanum lycopersicum L.) – 16 cultivars and two local genotypes – regenerated for the active collection of the National Institute for Horticultural Research. The plants were grown in the field in accordance with the adopted methodology of seed reproduction in the collection of genetic resources of vegetable plants. Tomato fruits were harvested twice a week from July 18 to September 26, 2019. Immediately after harvest, the seeds were extracted from the fruit, cleaned and dried according to FAO standards for gene banks. The seeds prepared in this way were stored for a period of sixty weeks at a temperature of 4±2°C in airtight containers with the addition of silica gel. During this period, after twenty, forty and sixty weeks of storage, their germination was tested. The studied tomato genotypes differed significantly in terms of depth of primary dormancy and rate of releasing from dormancy. Two of the tested cultivars – ‘Parteno’ and ‘Reper’, were characterized by seed dormancy at the level of 44.5% and 35.5%, two genotypes – ‘Amber’ and the local genotype POLPOB17-27 were characterized by dormancy levels of 15% and 18%. Seeds of two of the genotypes studied that did not show primary dormancy twenty weeks after harvest – ‘Zealand’ and the local genotype POLPOB17-28 – after storage for sixty weeks showed a reduced germination rate and a longer mean germination time. It is concluded that the optimum time for testing the germination of tomato seeds stored after harvest at 4 °C is forty weeks after harvest. The level of primary dormancy of the seeds is then the lowest, but the induction of secondary dormancy does not occur yet. When testing the seed germination in an earlier period, dormancy breaking procedures should be considered to obtain correct results.
    Źródło:
    Zeszyty Naukowe Instytutu Ogrodnictwa; 2021, 29; 15-24
    2300-5882
    2391-8969
    Pojawia się w:
    Zeszyty Naukowe Instytutu Ogrodnictwa
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Kolekcja aktywna nasion zasobów genowych roślin ogrodniczych w Regionalnym Centrum Bioróżnorodności Ogrodniczej Instytutu Ogrodnictwa - Państwowego Instytutu Badawczego w Skierniewicach
    Active collection of seeds of genetic resources of horticutular plants in the Rgional Centre for Horticulatural Biodiversity of the National Institute of Horticultural Research in Skierniewice
    Autorzy:
    Chojnowski, M.G.
    Dostatny, D.F.
    Bakalarska, A.
    Kapusta, E.
    Szyszkowska, K.
    Powiązania:
    https://bibliotekanauki.pl/articles/28763063.pdf
    Data publikacji:
    2022
    Wydawca:
    Instytut Ogrodnictwa
    Tematy:
    bank nasion
    bank nasion aktywny
    rosliny ogrodnicze
    Instytut Ogrodnictwa w Skierniewicach
    Źródło:
    Zeszyty Naukowe Instytutu Ogrodnictwa; 2022, 30; 1-14
    2300-5882
    2391-8969
    Pojawia się w:
    Zeszyty Naukowe Instytutu Ogrodnictwa
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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