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Wyszukujesz frazę "Mierzejewska, E.J." wg kryterium: Autor

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    Tytuł:
    Molecular study of transovarial transmission of Babesia canis in the Dermacentor reticulatus tick
    Autorzy:
    Mierzejewska, E.J.
    Dwużnik, D.
    Bajer, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2081946.pdf
    Data publikacji:
    2018
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Dermacentor reticulatus
    Babesia canis
    transovarial transmission
    Opis:
    The Dermacentor reticulatus tick is a main vector of Babesia canis in Europe. The risk of canine babesiosis is unpredictable, due to significant differences in the prevalence of B. canis between ticks originating from closely situated regions. This phenomenon may be explained by vertical transmission of the pathogen in a vector population. Thus, molecular techniques were applied to investigate the occurrence of transovarial transmission in D. reticulatus ticks. DNA of B. canis was detected in 20.7% (6/29) of engorged female ticks collected from dogs, in every pool of eggs laid by positive females (100%, 6/6) and in larvae hatched from these eggs. In the pools of eggs collected from two positive females (2/6; 33.3%), no larvae hatched and no embryos were observed inside the eggs. Conclusions. Transovarial transmission of B. canis can be an important mechanism supporting maintenance of the pathogen in the environment without the presence of a reservoir vertebrate host. However, the efficiency of transovarial transmission in the maintenance of B. canis in natural conditions requires further field research.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2018, 25, 4; 669-671
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Comparison of the detection efficiency of haemoparasite DNA in blood and faecal samples – the way to eco-epidemiological studies
    Autorzy:
    Bajer, A.
    Dwużnik, D.
    Tołkacz, K.
    Alsarraf, M.
    Mierzejewska, E.J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2085115.pdf
    Data publikacji:
    2019
    Wydawca:
    Instytut Medycyny Wsi
    Tematy:
    Babesia canis
    Babesia microti
    Babesia vulpes
    Hepatozoon canis
    red fox
    faecal samples
    faeces
    Opis:
    Introduction and objective. It is easier and non-invasive to obtain faecal samples compared with blood samples. Molecular techniques may enable detection of parasites even in tiny amounts of blood-containing faeces. We aimed to compare the sensitivity of detection of three Babesia species and Hepatozoon canis in blood and faecal samples, including samples derived from naturally infected hosts. Materials and method. Three groups were involved: 1) Nine BALB/c mice infected with Babesia microti sampled during acute (n=3), post-acute (n=3) and chronic phases of infection (n=3); 2) Eight dogs with symptoms of babesiosis; 3) Six red foxes infected with B. vulpes, one fox infected with B. canis, four foxes infected with H. canis. Genomic DNA was extracted from blood and faeces by use of commercial kits and amplified with genus-specific primers in one-step or nested PCR reactions. Selected PCR products were sequenced. Results. No positive results for faecal samples were obtained from H. canis-positive foxes in contrast to Babesia spp. infections. Positive results from PCRs were obtained for all BALB/c mice (100%), five dogs (62.5%) and four of seven foxes (57.1%). Successful sequencing was obtained for six selected murine samples (B. microti), four canine samples (B. canis) and for one fox sample (B. vulpes). The success of B. microti detection in murine faecal samples from acute, post-acute and chronic phases was identical (100%). Conclusions. Detectability of Babesia spp. infections was lower in naturally infected dogs and foxes, compared to experimentally infected mice. Detection of DNA in faecal samples can be useful in the detection of Babesia infection in populations from which blood samples are hard to obtain, but due regard must be given to the possibility that prevalence of infection may be severely underestimated.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2019, 26, 4; 538-543
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The first evidence for vertical transmission of Babesia canis in a litter of Central Asian Shepherd dogs
    Autorzy:
    Mierzejewska, E.J.
    Welc-Faleciak, R.
    Bednarska, M.
    Rodo, A.
    Bajer, A.
    Powiązania:
    https://bibliotekanauki.pl/articles/49587.pdf
    Data publikacji:
    2014
    Wydawca:
    Instytut Medycyny Wsi
    Opis:
    Introduction and objective. Tick-borne infections constitute an increasing health problem in dogs and may lead to death, especially in young or elderly individuals. Canine babesiosis constitutes a serious health problem in dogs worldwide. The aim of the study was to verify the probability of vertical transmission of Babesia canis between the bitch and the pups. Materials and methods. In Autumn 2011, cases of babesiosis were diagnosed in a litter of 6-week-old puppies of a Central Asian Shepherd dog. Immediately following the first case of infection, blood samples were collected from all the pups in the litter (n=10) and from the female. Detection of Babesia infection was performed by molecular and microscopical techniques. Results. The presence of B. canis DNA was detected using PCR in three pups, presenting at the time or 24–48 hours later with babesiosis symptoms, and in their asymptomatic mother. The isolates derived from the pups and the female – 520 bp 18S rRNA gene fragment – were compared and analyzed. All isolates from the pups and their mother were identical and showed 100% homology with B. canis group B (EU622793), supporting the same source of infection. Additionally, the USG of the peritoneal cavity was performed in the female, presenting evidence for splenomegaly. Conclusions. On the basis of (1) the same timing of three pup cases; (2) the identical B. canis sequences derived from all positive dogs; (3) evident splenomegaly in the asymptomatic female, this provides the first evidence of the vertical transmission of this piroplasm in dogs.
    Źródło:
    Annals of Agricultural and Environmental Medicine; 2014, 21, 3
    1232-1966
    Pojawia się w:
    Annals of Agricultural and Environmental Medicine
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
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