- Tytuł:
- The usage of antibiotics for prevention of contamination in 3T3-L1 preadipocyte culture
- Autorzy:
-
Ariyanto, Eko Fuji
Wikayani, Tenny Putri
Qomarilla, Nurul - Powiązania:
- https://bibliotekanauki.pl/articles/1076667.pdf
- Data publikacji:
- 2019
- Wydawca:
- Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
- Tematy:
-
3T3-L1 preadipocytes
antibiotics
contamination - Opis:
- Background: 3T3-L1 preadipocyte is the most commonly used cell line in in vivo studies of obesity. One of the main concerns in 3T3-L1 preadipocyte culture is microorganism contaminations. The objective of this study was to determine the appropriate antibiotics to prevent contaminations in 3T3-L1 cultures. Method: This study used descriptive analysis. Frozen 3T3-L1 preadipocytes were thawed and cultured in DMEM-10% FBS-1% penicillin-streptomycin, DMEM-10% FBS-1% penicillin-streptomycin-fungiezone, or DMEM-10% FBS-0.2% ciprofloxacin 200 mg/100 ml. After 24-hour incubation, the cells were observed under the microscope for any change in the medium colour, presence of abnormal structures, and abnormality in cell morphology. Results: The usage of 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml maintained the clean medium and conserved normal fibroblast-like morphology of the cells. Conclusion: This study suggested that 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml can be utilized in 3T3-L1 preadipocyte cultures to prevent contaminations.
- Źródło:
-
World News of Natural Sciences; 2019, 26; 218-223
2543-5426 - Pojawia się w:
- World News of Natural Sciences
- Dostawca treści:
- Biblioteka Nauki
Artykuł