The usage of antibiotics for prevention of contamination in 3T3-L1 preadipocyte culture

Background: 3T3-L1 preadipocyte is the most commonly used cell line in in vivo studies of obesity. One of the main concerns in 3T3-L1 preadipocyte culture is microorganism contaminations. The objective of this study was to determine the appropriate antibiotics to prevent contaminations in 3T3-L1 cultures. Method: This study used descriptive analysis. Frozen 3T3-L1 preadipocytes were thawed and cultured in DMEM-10% FBS-1% penicillin-streptomycin, DMEM-10% FBS-1% penicillin-streptomycin-fungiezone, or DMEM-10% FBS-0.2% ciprofloxacin 200 mg/100 ml. After 24-hour incubation, the cells were observed under the microscope for any change in the medium colour, presence of abnormal structures, and abnormality in cell morphology. Results: The usage of 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml maintained the clean medium and conserved normal fibroblast-like morphology of the cells. Conclusion: This study suggested that 1% penicillin-streptomycin, 1% penicillin-streptomycin-fungiezone, or 0.2% ciprofloxacin 200 mg/100 ml can be utilized in 3T3-L1 preadipocyte cultures to prevent contaminations.