Temat: sperm - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Effects of seminal plasma concentration on sperm motility and plasma and acrosome membrane integrity in chilled canine spermatozoa
Autorzy:: Pan, C.
Wu, Y.
Yang, Q.
Ye, J.
Powiązania:: https://bibliotekanauki.pl/articles/2087767.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: seminal plasma
chill
canine
sperm
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 1; 133-138
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Influence of splicing mutation within the lysophosphatidic acid receptor 1 gene (LPAR1) on semen quality in Holstein-Friesian bulls
Autorzy:: Kamiński, S.
Hering, D.M.
Kordan, W.
Lecewicz, M.
Sazanov, A.
Powiązania:: https://bibliotekanauki.pl/articles/2087732.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bull
sperm
quality
LPAR1
polymorphism
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 2; 419-421
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Activity of resveratrol on the influence of aflatoxin B1 on the testes of Sprague dawley rats
Autorzy:: Omur, A.D.
Yildirim, B.
Saglam, Y.S.
Comakli, S.
Ozkaraca, M.
Powiązania:: https://bibliotekanauki.pl/articles/2087503.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: aflatoxin B1
rat
resveratrol
sperm
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 313-320
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Quality parameters and fertility of ram semen cryopreserved in egg yolk and soybean lecithin supplemented extenders
Autorzy:: Gogol, P.
Bryła, M.
Trzcińska, M.
Bochenek, M.
Powiązania:: https://bibliotekanauki.pl/articles/2087594.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ram semen
cryopreservation
soybean lecithin
sperm quality
fertility
Źródło:: Polish Journal of Veterinary Sciences; 2019, 1; 177-179
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Genome-wide association studies of cryostability of semen in roosters
Autorzy:: Dementieva, N.V.
Kudinov, A.A.
Pozovnikova, M.V.
Nikitkina, E.V.
Pleshanov, N.V.
Silyukova, Y.L.
Krutikova, A.A.
Plemyashov, K.V.
Powiązania:: https://bibliotekanauki.pl/articles/2087292.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Genome-Wide Association Studies
cryostability
rooster sperm
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 461-463
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed spermin Holstein-Friesian bulls
Autorzy:: Kaminski, S.
Hering, D.M.
Kordan, W.
Lecewicz, M.
Powiązania:: https://bibliotekanauki.pl/articles/2087549.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bull
sperm
polymorphism
CFTR gene
antioxidant enzyme activity
Źródło:: Polish Journal of Veterinary Sciences; 2019, 2; 221-225
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: Method agreement between three different chambers for comparative bull semen computer assisted sperm motility analysis
Autorzy:: Basioura, A.
Tsousis, G.
Boscos, C.
Lymberopoulos, A.
Tsakmakidis, I.
Powiązania:: https://bibliotekanauki.pl/articles/2087300.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: analysis chamber
bull semen
computer assisted sperm analysis
motility
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 325-331
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Royal jelly protection on flunixin meglumine-induced spermiotoxicity and testicular degeneration in mice
Autorzy:: Temamoğulları, F.
Aral, F.
Yılmaz, R.
Powiązania:: https://bibliotekanauki.pl/articles/2087694.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: flunixin meglumine
royal jelly
male mice
sperm
testes toxicity
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 3; 497-506
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Evaluation of the effects of photostimulation on freeze-thawed bull sperm cells in terms of reproductive potential
Autorzy:: Ömür, A.D.
Powiązania:: https://bibliotekanauki.pl/articles/16539165.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: in vitro analysis
laser
LED (Light Emitting Diode)
photostimulation
sperm cell
Opis:: The aim of this study was to determine the time-dependent effectiveness of photo-stimulation against bovine sperm cells using a multi-wavelength LED (Light Emitting Diode). Spermatological parameters were evaluated for viability, acrosome structure and motility. In addition, the effect of photo-stimulation on frozen-thawed sperm cells subjected to in vitro capacitation was evaluated in terms of changes in mitochondrial membrane potential. The study consisted of two separate experiments and a total of 32 sperm samples obtained from separate bulls were used. All sperm samples were obtained from Holstein bulls using an artificial vagina. Semen was diluted to a final concentration of 92 x 106 spermatozoa per ml in 0.25 ml straws. The sperm cells were frozen using the conventional method. Straws were kept in a 37°C water bath for 20 seconds and diluted 1:4 in phosphate buffered saline (PBS) to eliminate the potentially deleterious effect of glycerol, the main permeable cryoprotectant in the freezing medium for bull sperm. This dilution also helped in the evaluation of sperm quality parameters. In the first experiment, whereas the 15-10-15 showed no differences with the control, other treatments such as 10-10-10, 5-5-5, and 3-1-3 exhibited significantly higher percentages of viable spermatozoa at 24h. The results obtained for acrosome integrity were pretty much similar to those observed in the sperm viability assessment. In effect, while the treatment consisting of 15-10-15 had no positive effects, shorter treatments exerted a much more positive effect. The percentages of acrosome-intact spermatozoa in 2-1-2 and 1-1-1 were significantly higher than those obtained in the control. The significant differences in mitochondrial membrane potential were observed at 0, 2, 4 and 24h post-photo-stimulation in all treatments, except 15-10-15. The highest increase in the percentage of spermatozoa exhibiting high mitochondrial membrane potential was found in 10-10-10, 5-5-5 and 3-1-3 treatments. With regard to total and progressive motility, whereas 10-10-10 was the best regime, 5-5-5 and 3-1-3 treatments also had a positive effect. However, 15-10-15 appeared to have a stimulating effect upon progressive motility at 2h and 4h but later declined and showed no significant differences with regard to the control at 24h. In the second experiment, not immediately after thawing but after having been kept at room temperature for up to 24h, it was observed that there was no statistical difference in terms of viability, acrosome integrity and total/progressive motility between photostimulation and the control group. This indicates that photo- stimulation is less able to exert a beneficial effect when post-thawed sperm are not immediately stimulated. As a result it was determined that photo-stimulation at a pattern of 10-10-10, 5-5-5, 3-1-3 and, to a lesser extent 2-1-2, increases the resilience of frozen-thawed bull sperm when applied upon thawing.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 2; 249-259
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: The quality and fertilizing ability of the ram semen cryopreserved around 40 years ago
Autorzy:: Gogol, P.
Powiązania:: https://bibliotekanauki.pl/articles/16647392.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: ram semen
ex situ storage
gene banks
cryopreservation
sperm quality
fertility
Opis:: The aim of the study was to verify the quality of ram semen, frozen in 1982-1983, from the historical collection of the Bank of Biological Material of the National Research Institute of Animal Production. A total of 18 ejaculates from 3 Świniarka type rams were analyzed to assess sperm motility (subjectively), total motility, progressive motility, sperm concentration (CASA), membrane integrity (SYBR-14/PI) and chromatin structure (SCSA). In order to determine sperm fertilizing ability 49 ewes were intracervically inseminated (200×106 sperm per AI) with frozen- thawed semen 12 and 24 hours after detection of estrus. Sperm motility parameters, membrane intact spermatozoa and DFI did not differ among the analyzed rams. Spermatozoa concentration was significantly higher for ram no. 2 than for rams no. 1 and 3. The lambing rates (27.3 to 36.0%) did not differ significantly for individual rams. The ram semen, which had been stored for around 40 years, showed satisfactory quality and fertilizing capacity, allowing for its use in artificial insemination.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 4; 631-633
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Effect of sperm concentration in an ejaculate on morphometric traits of spermatozoa in Duroc boars
Autorzy:: Kondracki, S.
Wysokinska, A.
Iwanina, M.
Banaszewska, D.
Sitarz, D.
Powiązania:: https://bibliotekanauki.pl/articles/31784.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: sperm concentration
ejaculate
morphometry
spermatozoon
Duroc boar
boar
morphology
animal reproduction
mammal
Opis:: The experimental material consisted of 75 ejaculates collected form 8 Duroc boars. The ejaculates were divided into three groups according to sperm concentration in an ejaculate. An ejaculate was obtained from each boar monthly and it was used to make microscopic preparations to examine spermatozoa morphology. In each preparation morphometric measurements were taken of fifteen randomly selected spermatozoa characterized by normal morphology. The following measurements of spermatozoa were taken: length and width of the spermatozoa head, head area, length of the flagellum, perimeter of the spermatozoon head and total spermatozoon length. The results were used to calculate indicators of spermatozoa morphology. Moreover, assessments were made of frequency of morphological defects to isolate spermatozoa with primary and secondary abnormalities following the Blom classification system. It was found that the concentration of spermatozoa in the ejaculate influenced the morphometric characteristics of spermatozoa. Ejaculates with low sperm concentrations are characterized by larger spermatozoa as compared to ejaculates with high sperm concentrations. However, sperm concentration in the ejaculate does not much influence the shape of spermatozoa.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Boar variability affects sperm metabolism activity in liquid stored semen at 5 degrees Celsius
Autorzy:: Dziekonska, A.
Strzezek, J.
Powiązania:: https://bibliotekanauki.pl/articles/30420.pdf
Data publikacji:: 2011
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: boar
metabolic activity
metabolism
semen
spermatozoon
sperm
variability
individual variation
temperature
cold shock
Opis:: Metabolic activity of boar spermatozoa, liquid stored for three days at 5℃, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5℃ may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.
Źródło:: Polish Journal of Veterinary Sciences; 2011, 14, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 13.

Tytuł:: Effect of platelet activating factor (PAF) supplementation in semen extender on viability and ATP content of cryopreserved canine spermatozoa
Autorzy:: Kordan, W.
Lecewicz, M.
Strzezek, R.
Dziekonska, A.
Fraser, L.
Powiązania:: https://bibliotekanauki.pl/articles/30804.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: platelet-activating factor
supplementation
semen
viability
spermatozoon
cryopreservation
cryopreserved spermatozoon
dog
adenosine triphosphate content
sperm
Opis:: The aim of this study was to investigate the effect of platelet activating factor (PAF) on the quality characteristics of cryopreserved canine spermatozoa. Cryopreserved semen of 5 mixed-breed dogs was treated with different concentrations of exogenous PAF (1 × 10⁻³M, 1 × 10⁻⁴M, 1 × 10⁻⁵M and 1 × 10-6M) and examined at different time intervals (0, 30, 60 and 120 min). Cryopreserved semen treated without PAF was used as the control. Sperm quality was evaluated for motility (computer-assisted semen analysis, CASA), mitochondrial function (JC-1/PI assay) and plasma membrane integrity (SYBR-14/PI assay and Hoechst 33258). Also, ATP content of spermatozoa was determined using a bioluminescence assay. Treatment of cryopreserved semen with 1 × 10⁻³ M PAF at 120 min of incubation resulted in significantly higher total sperm motility compared with the control. It was observed that PAF-improved total sperm motility was concurrent with enhanced sperm motility patterns after treatment of cryopreserved semen. Treatment of cryopreserved semen with PAF did not improve either sperm mitochondrial function or plasma membrane integrity, as monitored by different fluorescent membrane markers. Furthermore, ATP content of cryopreserved spermatozoa was significantly higher when PAF was used at a concentration of 1 × 10⁻³ M compared with the control and other PAF treatments, regardless of the incubation time. The findings of this study indicated that treatment with 1 × 10⁻³ M PAF at 120 min of incubation rendered better quality of cryopreserved canine semen, which was associated with improved sperm motility parameters and ATP content. It can be suggested that exogenous PAF addition is beneficial as a supplement for canine semen extender used for cryopreservation.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 14.

Tytuł:: Acrosin system of dog spermatozoa and reproductive tract secretions
Autorzy:: Strzezek, R
Koziorowska-Gilun, M.
Filipowicz, K.
Powiązania:: https://bibliotekanauki.pl/articles/32251.pdf
Data publikacji:: 2010
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: dog
semen
proacrosin
acrosin
spermatozoon
sperm-rich fraction
whole ejaculate
antitrypsin activity
antitrypsin inhibitor
seminal plasma
ejaculate
Beagle dog
mixed breed
Opis:: The aim of this study was to determine the activity of proacrosin and acrosin in spermatozoa originating from the sperm-rich fractions (SRF) and whole ejaculates (WE) of dog semen. In addition, experiments were conducted to determine the activity of antitrypsin inhibitors in the fluids of different ejaculate fractions and whole seminal plasma. Ejaculates were collected from five dogs of mixed breed and one Beagle dog (aged from 2 to 9 years). In the SRF, it was confirmed that the activity of the free acrosin form was predominant (acrosin / proacrosin; 2.38 ± 0.22 / 1.05 ± 0.08 mIU / 106 spermatozoa). On the other hand, spermatozoa originating from the WE exhibited significantly higher (p<0.05) proacrosin activity (proacrosin / acrosin; 2.19 ± 0.19 /1.30 ± 0.11 mIU / 106 spermatozoa). Furthermore, acrosin inhibitor activity was lower in the fluids of the pre-sperm fraction (0.09 ± 0.006 IU / cm3), whereas it was higher in the fluids of the post-sperm fraction (0.11 ± 0.007 IU / cm3). Using PAGE analysis, the antitrypsin activity of the enzyme was represented by the presence of one electrophoretic band in the fluids of the pre-sperm and post-sperm fractions and whole seminal plasma. Furthermore, two electrophoretic bands were detected in the fluids of the SRF. The findings of this study indicate that specific proteinase inhibitors present in the individual ejaculate fractions of dog semen may act by stabilizing the sperm acrosin system.
Źródło:: Polish Journal of Veterinary Sciences; 2010, 13, 1; 69-73
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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