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Wyświetlanie 1-15 z 16
Tytuł:
Isolation and sequence analysis of the complete VP2 gene of canine parvovirus from Chinese domestic pets and determination of the pathogenesis of these circulating strains in beagles
Autorzy:
Chen, M.R.
Guo, X.Y.
Wang, Z.Y.
Jiang, Y.T.
Yuan, W.F.
Xin, T.
Hou, S.H.
Song, T.Q.
Lin, W.D.
Zhu, H.F.
Jia, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087542.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
canine parvovirus
molecular epidemiology
phylogenetic analysis
pathogenesis
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 287-296
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Vertical transmission of PCV2b to fetuses in sows intramuscularly infected with PCV2b
Autorzy:
Wang, N.D.
Li, J.J.
Wang, A.B.
Zhu, Z.
Chang, Y.
Mao, Y.
Zan, Y.
Wang, Z.F.
Deng, Z.B.
Powiązania:
https://bibliotekanauki.pl/articles/31535.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In order to investigate whether PCV2b can be transmitted across the placenta in sows thereby causing vertical infection of fetuses, six sows in 75 day of pregnancy were either intramuscularly inoculated with a PCV2b culture supernatant (n = 4) or mock infected with cell culture supernatant (n = 2). At parturition, 3 newborn piglets from each litter were randomly selected and euthanatized prior to suckling. Samples of the mesenteric lymph nodes, spleens, kidneys, hearts and lungs were collected for PCR, histopathological and immunohistochemical assays. The results showed that the newborn piglets from PCV2b-infected sows had histopathological lesions in the tested tissues. Moreover, PCV2b antigen and DNA were detected as well. These findings therefore suggested that porcine circovirus type 2b can be transmitted across the placenta of sows, thereby leading to PCV2b vertical infection of the fetuses.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomics profiles reveal the potential roles of proteins involved in chicken macrophages stimulated by Lipopolysaccharide
Autorzy:
Li, S.
Chen, Y.
Xue, W.
Wang, Q.
Huai, Z.
An, C.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16647586.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
chicken macrophage (HD11)
immune response
lipopolysaccharide (LPS)
proteomics
Opis:
Lipopolysaccharide (LPS), a core part of gram-negative bacteria, is crucial for inducing an inflammatory response in living things. In the current study, we used LPS from Salmonella to stimulate chicken macrophages (HD11). Proteomics was used to investigate immune-related proteins and their roles further. Proteomics investigation revealed 31 differential expression proteins (DEPs) after 4 hours of LPS infection. 24 DEPs expressions were up-regulated, while seven were down-regulated. In this investigation, ten DEPs were mainly enriched in S. aureus infection, complement, and coagulation cascades, which were all implicated in the inflammatory response and clearance of foreign pathogens. Notably, complement C3 was shown to be up-regulated in all immune-related pathways, indicating that it is a potential protein in this study. This work contributes to a better understanding and clarification of the processes of Salmonella infection in chickens. It might bring up new possibilities for treating and breeding Salmonella-infected chickens.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 2; 265-274
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
Autorzy:
Yu, H.
Zhang, L.
Cai, Y.
Hao, Z.
Luo, Z.
Peng, T.
Liu, L.
Wang, N.
Wang, G.
Deng, Z.
Zhan, Y.
Powiązania:
https://bibliotekanauki.pl/articles/16539345.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
classical swine fever virus (CSFV)
porcine reproductive and respiratory syndrome virus (PRRSV)
pig
seroprevalence
antibody
Opis:
Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro
Autorzy:
Zhu, X.
Zhou, L.
Liu, Z.
Chen, X.
Wei, L.
Zhang, Z.
Liu, Y.
Zhu, Y.
Wang, Y.
Yang, X.
Han, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087304.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
bone marrow mesenchymal stem cells
telomerase
proliferation
osteogenic differentiation
PI3K/Akt signalling pathway
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 359-372
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
SYBR Green-based real-time polymerase chain reaction assay for detection of porcine parvovirus 6 in pigs
Autorzy:
Sun, P.
Bai, C.X.
Zhang, D.
Wang, J.
Yang, K.K.
Cheng, B.Z.
Li, Y.D.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087328.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
porcine parvovirus 6
real-time polymerase chain reaction
SYBR Green
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 197-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:
Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:
Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:
Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:
https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of a rapid immunochromatographic assay for detection of antibodies against porcine epidemic diarrhea virus
Autorzy:
Li, R.
Ma, J.
Tian, X.
Yu, Y.
Qiao, S.
Wang, Z.
Powiązania:
https://bibliotekanauki.pl/articles/2087760.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
porcine epidemic diarrhea virus
immunochromatographic test strip
nucleocapsid protein
diagnosis
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 139-147
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
Autorzy:
Yang, Z.
Wang, K.
Hu, Y.
Yuan, P.
Yang, Y.
Xie, L.Y.
Huang, S.L.
Liu, J.
Ran, L.
Song, Z.H.
Powiązania:
https://bibliotekanauki.pl/articles/2087759.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
swine HEV
ORF2
baculovirus
immunogenicity
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method for tracing exogenous DNA uptake in live spermatozoa and embryos
Autorzy:
Mu, Y.
Wang, J.
Liu, Z.
Zhao, Y.
Zhang, X.
Jiao, M.
Lv, J.
Hao, J.
Kong, Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087751.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
spermatozoa
SMGT
exogenous DNA
IVF
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 193-202
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Molecular cloning, expression, purification and osteoblasts proliferation activity of sika deer thymosin beta10
Autorzy:
Zhao, D.
Zhao, Y.
Zhang, H.
Xu, Z.
Liu, M.
Wang, J.
Bai, X.
Powiązania:
https://bibliotekanauki.pl/articles/2087874.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
affinity chromatography
cell proliferation
prokaryotic expression
sika deer
thymosin 10
Źródło:
Polish Journal of Veterinary Sciences; 2017, 4; 751-758
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:
Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
Autorzy:
Wu, X.
Xiao, L.
Peng, B.
Wang, Y.
Yang, Z.
Yao, X.
Hu, L.
Lin, X.
Powiązania:
https://bibliotekanauki.pl/articles/30963.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
Autorzy:
Pei, Z.
Ying, X.
Tang, Y.
Liu, L.
Zhang, H.
Liu, S.
Zhang, D.
Wang, K.
Kong, L.
Gao, Y.
Ma, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087656.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antimicrobial peptide
biological characteristics
antibacterial mechanism
Gram-negative bacteria
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 16

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