Autor: Wang, Y.-L. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Perspectives on novel vaccine development
Autorzy:: Wang, Y.B.
Wang, L.P.
Li, P.
Powiązania:: https://bibliotekanauki.pl/articles/2087687.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: vaccination
vaccinology
novel vaccine development
perspectives
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 3; 643-649
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Emergence and complete genome of Senecavirus A in pigs of Henan Province in China, 2017
Autorzy:: Wang, H.B.
Tian, B.
Lv, H.L.
Wang, F.
Zhang, T.
Wang, C.Y.
Zhang, Y.D.
Dong, J.J.
Powiązania:: https://bibliotekanauki.pl/articles/2087575.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Senecavirus A
vesicular disease
phylogenetic analysis
Źródło:: Polish Journal of Veterinary Sciences; 2019, 1; 187-190
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China
Autorzy:: Yu, H.
Zhang, L.
Cai, Y.
Hao, Z.
Luo, Z.
Peng, T.
Liu, L.
Wang, N.
Wang, G.
Deng, Z.
Zhan, Y.
Powiązania:: https://bibliotekanauki.pl/articles/16539345.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: classical swine fever virus (CSFV)
porcine reproductive and respiratory syndrome virus (PRRSV)
pig
seroprevalence
antibody
Opis:: Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 3; 375-381
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Telomerase enhances osteogenic ifferentiation of sheep bone marrow mesenchymal stem cells (BMSCs) by up-regulating PI3K/Akt pathway in vitro
Autorzy:: Zhu, X.
Zhou, L.
Liu, Z.
Chen, X.
Wei, L.
Zhang, Z.
Liu, Y.
Zhu, Y.
Wang, Y.
Yang, X.
Han, Y.
Powiązania:: https://bibliotekanauki.pl/articles/2087304.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bone marrow mesenchymal stem cells
telomerase
proliferation
osteogenic differentiation
PI3K/Akt signalling pathway
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 359-372
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
Autorzy:: Yang, Z.
Wang, K.
Hu, Y.
Yuan, P.
Yang, Y.
Xie, L.Y.
Huang, S.L.
Liu, J.
Ran, L.
Song, Z.H.
Powiązania:: https://bibliotekanauki.pl/articles/2087759.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: swine HEV
ORF2
baculovirus
immunogenicity
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 6.

Tytuł:: Magnolol inhibits Streptococcus suis-induced inflammation and ROS formation via TLR2/MAPK/NF-κB signaling in RAW264.7 cells
Autorzy:: Wang, J.
Sun, Y.
Zhang, L.
Xu, W.
You, J.
Lu, H.
Song, Y.
Wei, J.
Li, L.
Powiązania:: https://bibliotekanauki.pl/articles/2087779.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: magnolol
Streptococcus suis
Toll-like receptor2
mitogen-activated protein kinase
nuclear factor Kappa B
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 1; 111-118
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 7.

Tytuł:: A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:: Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:: https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 8.

Tytuł:: Development of a SYBR Green I real-time PCR assay for detection of novel porcine parvovirus 7
Autorzy:: Li, Y.D.
Yu, Z.D.
Bai, C.X.
Zhang, D.
Sun, P.
Peng, M.L
Liu, H.
Wang, J.
Wang, Y.
Powiązania:: https://bibliotekanauki.pl/articles/2087207.pdf
Data publikacji:: 2021
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: Capsid gene
PPV7
SYBR Green I real-time PCR
Źródło:: Polish Journal of Veterinary Sciences; 2021, 24, 1; 43-49
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 9.

Tytuł:: Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
Autorzy:: Wu, X.
Xiao, L.
Peng, B.
Wang, Y.
Yang, Z.
Yao, X.
Hu, L.
Lin, X.
Powiązania:: https://bibliotekanauki.pl/articles/30963.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
Źródło:: Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 10.

Tytuł:: Biological characteristics of a new antibacterial peptide and its antibacterial mechanisms against Gram-negative bacteria
Autorzy:: Pei, Z.
Ying, X.
Tang, Y.
Liu, L.
Zhang, H.
Liu, S.
Zhang, D.
Wang, K.
Kong, L.
Gao, Y.
Ma, H.
Powiązania:: https://bibliotekanauki.pl/articles/2087656.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: antimicrobial peptide
biological characteristics
antibacterial mechanism
Gram-negative bacteria
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 3; 533-542
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 11.

Tytuł:: Pharmacokinetics of the recombinant ovine interferon-tau in lambs
Autorzy:: Zhao, J.
Yu, H.Y.
Zhao, Y.
Li, S.Q.
Fu, X.L.
Zhou, W.
Xia, B.B.
Wang, M.L.
Chen, J.
Powiązania:: https://bibliotekanauki.pl/articles/2087588.pdf
Data publikacji:: 2019
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: bioavailability
ovine interferon-τ
cytopathic effect inhibition assay
pharmacokinetic study
Źródło:: Polish Journal of Veterinary Sciences; 2019, 1; 75-82
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 12.

Tytuł:: Rapid screening of monoclonal antibodies against porcine circovirus type 2 using colloidal gold-based paper test
Autorzy:: Jin, Q.Y.
Feng, L.L.
Wang, Y.B.
Li, P.
Yang, J.F.
Teng, M.
Chai, S.J.
Xing, G.X.
Zhang, G.P.
Powiązania:: https://bibliotekanauki.pl/articles/16539073.pdf
Data publikacji:: 2022
Wydawca:: Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:: porcine circovirus type 2 (PCV2)
screening of MAbs
paper test
Opis:: A proof of concept for using paper test as a suitable method in the production of monoclonal antibodies (MAbs) is reported. The paper test which detects antibodies against porcine circovirus type 2 (PCV2) using colloidal gold-labelled capsid protein as the antigen probe was applied exclusively in the screening of anti-PCV2 MAbs. It allowed the detection of 118 single cell clones within 30 min using naked eyes. MAbs with specific binding to authentic epitopes on the virus were selected using a blocking strategy in which the antibody was pre-incubated with PCV2 viral sample before applying to the test paper. Five hybridomas secreting MAbs against the capsid protein were obtained, with only three of them capable of binding to PCV2. The results were validated and confirmed using enzyme-linked immunosorbent assay and immunofluorescence assay. The paper test is simple, rapid, and independent on professional technicians and proves to be an excellent approach for the screening of MAbs against specific targets.
Źródło:: Polish Journal of Veterinary Sciences; 2022, 25, 1; 27-34
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki