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Tytuł:
Changes in expression of calbindin 28 kDa in the small intestine of red kidney bean (Phaseolus vulgaris) lectin-treated suckling piglets
Autorzy:
Zacharko-Siembida, A.
Valverde Piedra, J.L.
Arciszewski, M.B.
Powiązania:
https://bibliotekanauki.pl/articles/30868.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Calbindin (CB) is a calcium binding protein playing a role in calcium uptake and anti-apoptotic cellular protection. The expression of CB was immunohistochemically studied in the small intestine of normal and red bean kidney lectin-treated suckling piglets. In the duodenum and jejunum (but not ileum) of lectin-treated animals overexpression of CB was noted in chromogranin A-immunoreactive (CgA-IR) neuroendocrine (NE) cells. In both control and experimental group a small population of CB-IR NE cells exhibited the presence of somatostatin (but not serotonin, histamine or CRF). After the lectin treatment, an increased (however not statistically significant) immunoreactivity to CB was found in a small subpopulation of neurons of outer submucous (but not inner submucous and myenteric) plexus. It is suggested that there is a functional interaction between lectin administration and CB-expression in the porcine small intestine. Future studies will be needed to clarify this processes.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of a handheld device for the measurement of beta-hydroxybutyrate in capillary blood obtained by the puncture of the vulva as well as in venous whole blood in cattle
Autorzy:
Khol, J.L.
Freigassner, K.
Stanitznig, A.
Tichy, A.
Wittek, T.
Powiązania:
https://bibliotekanauki.pl/articles/2087481.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
beta-hydroxybutyrate
subclinical ketosis
capillary blood
handheld device
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 557-564
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prevalence of pathogenicity island ETT2 in Escherichia coli isolated from piglets with diarrhea in northeast of China
Autorzy:
Yuan, C.W.
Liu, W.X.
Hou, J.L.
Zhang, L.G.
Wang, G.Q.
Powiązania:
https://bibliotekanauki.pl/articles/2087783.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Escherichia coli
pathogenicity island
ETT2
piglets
diarrhea
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 5-12
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Indirect enzyme-linked immunosorbent assay method based on Streptococcus agalactiae rSip-Pgk-FbsA fusion protein for detection of bovine mastitis
Autorzy:
Bu, R.-E.
Wang, I.-L.
Wu, J.-H.
Xilin, G.W.
Chen, J.-L.
Wang, H.
Powiązania:
https://bibliotekanauki.pl/articles/30156.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Objective: The aim of this study was to establish a rapid and accurate method for the detection of the Streptococcus agalactiae antibody (SA-Ab) to determine the presence of the bovine mastitis (BM)-causative pathogen. Methods: The multi-subunit fusion protein rSip-Pgk-FbsA was prokaryotically expressed and purified. The triple activities of the membrane surface-associated proteins Sip, phosphoglycerate kinase (Pgk), and fibronectin (FbsA) were used as the diagnostic antigens to establish an indirect enzyme-linked immunosorbent assay (ELISA) method for the detection of SA-Ab in BM. Results: The optimal antigen coating concentration was 2 μg/mL, the optimal serum dilution was 1:160, and the optimal dilution of the enzyme-labeled secondary antibody was 1:6000. The sensitivity, specificity, and repeatability tests showed that the method established in this study had no cross-reaction with antibodies to Streptococcus pyogenes, Escherichia coli, Staphylococcus aureus, and Staphylococcus epidermidis in the sera. The results of the sensitivity test showed that a positive result could be obtained even if the serum dilution reached 1:12,800, indicating the high sensitivity and good repeatability of the method. The positive coincidence rate of this method was 98.6%, which is higher than that of previous tests established with the Sip or Pgk mono-antigen fusion protein, respectively, demonstrating the relatively higher sensitivity of this newly established method. The detection rate for 389 clinical samples was 46.53%. Conclusions: The indirect ELISA method established in this study could provide a more accurate and reliable serological method for the rapid detection of S. agalactiae in cases of BM.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage
Autorzy:
Jovicić, M.
Pintus, E.
Fenclova, T.
Simonik, O.
Chmelikova, E.
Ros-Santaella, J. L.
Sedmikova, M.
Powiązania:
https://bibliotekanauki.pl/articles/2087766.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar spermatozoa
nitric oxide
nitric oxide synthases
NO donor
NOS inhibitor
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 73-82
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Expression and immunological evaluation of elongation factor Tu of Streptococcus suis serotype 2
Autorzy:
Xia, X.J.
Wang, L.
Cheng, L.K.
Shen, Z.Q.
Li, S.G.
Wang, J.L.
Powiązania:
https://bibliotekanauki.pl/articles/31256.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Streptococcus suis serotype 2 (SS2) is considered as a major pathogen that causes sepsis and meningitis in piglets and humans, but knowledge of its antigenic proteins remains limited so far. The surface-related proteins of pathogens often play significant roles in bacterium-host interactions and infection. Here, we obtained the elongation factor Tu (EF-Tu) gene of Streptococcus suis and constructed the recombinant expression plasmid successfully. The target recombinant plasmid was then expressed in Escherichia coli and the immuno-protection of the recombinant protein was subsequently evaluated as well. The EF-Tu gene of Streptococcus suis is 1197 bp in length, encodes 398 amino acids. The target recombinant EF-Tu (rEF-Tu) protein can recognize the antiserum of Streptococcus suis and can provoke obvious humoral immune responses in rabbits and conferred protection to rabbits against Streptococcus suis ear-vein challenge, implying that the EF-Tu may be used as an attractive candidate antigen for a component of subunit vaccine.
Źródło:
Polish Journal of Veterinary Sciences; 2017, 20, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Concentration levels of cadmium and lead in the raw and processed meat of Helix pomatia snails
Autorzy:
Ziomek, M.
Drozd, Ł.
Chałabis-Mazurek, A.
Szkucik, K.
Paszkiewicz, W.
Valverde Piedra, J. L.
Bełkot, Z.
Maćkowiak-Dryka, M.
Gondek, M.
Knysz, P.
Powiązania:
https://bibliotekanauki.pl/articles/2087661.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cadmium
lead
GFAAS
snail meat
Helix pomatia
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 483-489
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
17 beta-estradiol affects proliferation and apoptosis of canine bone marrow mesenchymal stem cells in vitro
Autorzy:
Zhou, Z.-H.
Gu, C.-W.
Li, J.
Huang, X.-Y.
Deng, J.-Q.
Shen, L.-H.
Cao, S.-Z.
Deng, J.-L.
Zuo, Z.-C.
Wang, Y.
Ma, X.-P.
Ren, Z.-H.
Yu, S.-M.
Powiązania:
https://bibliotekanauki.pl/articles/2087308.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
apoptosis
bone marrow mesenchymal stem cells (BMSCs)
canine
proliferation
17 beta-estradiol (E2)
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 2; 235-245
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Anti-babesial potential and chemical composition of essential oil from yarrow Achillea millefolium
Autorzy:
Guz, L.
Wawrzykowski, J.
Adaszek, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/2087200.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Achillea millefolium
anitbabesial activity
essential oil
Źródło:
Polish Journal of Veterinary Sciences; 2021, 24, 1; 79-84
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In vitro antioxidant and antibabesial activities of the extracts of Achillea millefolium
Autorzy:
Guz, L.
Adaszek, Ł.
Wawrzykowski, J.
Ziętek, J.
Winiarczyk, S.
Powiązania:
https://bibliotekanauki.pl/articles/2087537.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
yarrow
plant extracts
babesiosis
tick borne diseases
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 360-376
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Inhibitory activities of essential oils against Babesia canis
Autorzy:
Guz, L.
Ziętek, J.
Puk, K.
Adaszek, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/2087360.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Babesia canis
essential oil
natural product
Źródło:
Polish Journal of Veterinary Sciences; 2020, 23, 1; 161-163
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Experimental allogenic transplantation of cornea endothelial cells in cats
Autorzy:
Kielbowicz, Z.
Kuryszko, J.
Strzadala, L.
Powiązania:
https://bibliotekanauki.pl/articles/31702.pdf
Data publikacji:
2010
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
experimental transplantation
transplantation
cornea
endothelial cell
cat
endothelium
Opis:
Background: The aim of the present study was assessing the possibility of experimental allogenic transplantation of cat cornea endothelial cells, multiplied in vitro, into the anterior chamber of the eyeball in recipient cats. The reason for undertaking the research is the need to develop a method that would help in the cornea treatment in animals with corneal opacification following cataract surgery, as well as lens dislocation, injuries and endothelium degeneration. Methods: Cats aged 10-12 months were used in the experiment. Cornea fragments consisting of the posterior limiting membrane and posterior epithelium were placed in Iscove;s medium with addition of 10% foetal calf serum. Multiplied in vitro cells were injected into the anterior chamber of recipient cats. The cornea was subject to histological, histometric and SEM examination on the 3rd, 7th, 20th and 30th day after the surgery. Results: Micromorphological examination of the cornea showed full restitution of its endothelium 30 days after transplantation. Complete regeneration of structures indispensable for normal functioning of the posterior epithelium occurred as a result of implantation. Conclusions: In this study the results show that implantation of the cells of posterior corneal epithelium of donor cats, multiplied into vitro and injected into the anterior chamber of recipient cats. The cornea regained its full function, the layer of the posterior epithelium was regenerated and the stroma stabilized, presenting the image of full and proper corneal translucency.
Źródło:
Polish Journal of Veterinary Sciences; 2010, 13, 4
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of the anti-mycobacterial activity and composition of Carlina acaulis L. root extracts
Autorzy:
Puk, K.
Wawrzykowski, J.
Guz, L.
Powiązania:
https://bibliotekanauki.pl/articles/16647458.pdf
Data publikacji:
2023
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Carlina acaulis
mycobacterium
NTM
minimum inhibitory concentration
Opis:
Nontuberculous mycobacteria (NTM) have recently emerged as important bacterial pathogens of animals and humans. Of particular concern is the high level of antimicrobial resistance displayed by these organisms, which complicates treatment and potential successful outcomes. Here, we evaluated the potential of Carlina acaulis L. as a source of novel anti-mycobacterial agents. Our goal was to measure the activity of aqueous, ethanol, and chloroform C. acaulis root extracts against 99 NTM strains. GC-MS spectroscopy analyses were performed to deliver qualitative and quantitative data on the composition of C. acaulis extract. In our study, we have shown for the first time the activity of C. acaulis extracts against NTM. The highest activity was exhibited by the chloroform extract, which inhibited the growth of more than 90% of the strains at the dose of 100 μg/mL (MIC90 = 100 μg/mL). The results of the GC-MS analysis of the C. acaulis chloroform extract contributed to the identification of 37 compounds, with carlina oxide as the most representative compound (69.52%) followed by 3,4-dihydro-2H-phenanthren- -1-one (6.54%) and stigmast-5-en-3-ol (4.14%). Our results indicate that C. acaulis chloroform and ethanol extracts have potential for treatment of NTM infections and that this plant contains anti-mycobacterial compounds.
Źródło:
Polish Journal of Veterinary Sciences; 2023, 26, 1; 57-63
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Interactions of ostrich egg yolk lipoproteins with seminal plasma of fractionated ejaculates and their effects on post-thaw boar semen quality
Autorzy:
Fraser, L.
Zasiadczyk, Ł.
Strzeżek, J.
Kordan, W.
Mańkowska, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087484.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
boar semen
ejaculate fractions
egg yolk lipoproteins
cryopreservation
Źródło:
Polish Journal of Veterinary Sciences; 2019, 3; 463-473
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The concentration of urea in hemolymph as a marker of health in Lissachatina fulica and Cornu aspersum edible snails – a preliminary study
Autorzy:
Ziętek, J.
Guz, L.
Wójcik, A.
Winiarczyk, S.
Adaszek, Ł.
Powiązania:
https://bibliotekanauki.pl/articles/2087559.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Lissachatina fulica
Cornu aspersum
hemolymph
urea
markers
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 259-262
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł

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