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    Wyświetlanie 1-12 z 12
    Tytuł:
    Construction of a recombinant baculovirus expressing swine hepatitis E Virus ORF2 and preliminary research on its immune effect
    Autorzy:
    Yang, Z.
    Wang, K.
    Hu, Y.
    Yuan, P.
    Yang, Y.
    Xie, L.Y.
    Huang, S.L.
    Liu, J.
    Ran, L.
    Song, Z.H.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087759.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    swine HEV
    ORF2
    baculovirus
    immunogenicity
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 1; 47-54
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
    Autorzy:
    Shen, L.
    Qian, B.
    Xiao, J.
    Zhu, Y.
    Hussain, S.
    Deng, J.
    Peng, G.
    Zuo, Z.
    Zou, L.
    Yu, S.
    Ma, X.
    Zhong, Z.
    Ren, Z.
    Wang, Y.
    Liu, H.
    Zhou, Z.
    Cai, D.
    Hu, Y.
    Zong, X.
    Cao, S.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087289.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    adiponectin
    dairy cows
    ketosis
    leptin
    perinatal period
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of an indirect enzyme-linked immunosorbent assay for the detection of novel chicken orthoreovirus
    Autorzy:
    Wei, Z.
    Yang, J.
    Hu, J.
    Tang, Y.
    Diao, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16539093.pdf
    Data publikacji:
    2022
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    novel avian orthoreovirus
    σC gene
    recombinant plasmid
    indirect enzyme linked immunosorbent assay
    epidemiological investigation
    Opis:
    A novel avian orthoreovirus (N-ARV) variant characterized with obvious arthritis and synovial inflammation, was isolated from Shandong, China in May 2016. It caused chicken poor growth and enormous economic losses to the poultry industry of China. However, there are few effective methods for detecting the antibody levels of N-ARV. In this study, a viral structural protein σC was expressed using the prokaryotic expression vector pET32a (+). The target protein was obtained by inducing for 6 hours at an IPTG concentration of 0.6mM. The optimal dilution of the coating antigen and serum antibody were determined to be 1000 fold and 10 fold respectively. A specificity test showed that there was no positive reactivity between N-ARV and other pathogens, and when the positive serum was diluted 100 times detection results were still checkable. The repeatability of this method was determined by the inter assay and intra assay tests with variability ranging from 4.85% to 7.93%. In conclusion, this indirect enzyme linked immunosorbent assay (ELISA) will be useful for large-scale serological surveys and monitoring antibody levels in N-ARV infection.
    Źródło:
    Polish Journal of Veterinary Sciences; 2022, 25, 1; 109-118
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Detection of IL-1β, IL-6 and TNF-αin Sprague-Dawely rats’ atrophic thymus induced by lipopolysaccharide
    Autorzy:
    Zhong, Y.B.
    Zhang, X.L.
    Lv, M.Y.
    Hu, X.F.
    Li, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087664.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    lipopolysaccharide
    pro-inflammatory cytokines
    thymus atrophy
    rat
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 589-597
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    The expression profile of miR-222b-5p/MAPK10 in spleens of SPF chickens infected with REV-SNV at 28-42 dpi
    Autorzy:
    Jiang, H.
    Gao, S.
    Mao, M.
    Diao, Y.
    Tang, Y.
    Hu, J.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087117.pdf
    Data publikacji:
    2021
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    reticuloendotheliosis virus strain SNV
    RLRs signaling pathway
    gga-miR-222b-5p
    mitogen-activated protein kinase 10
    a dual-luciferase reporter gene experiment
    Źródło:
    Polish Journal of Veterinary Sciences; 2021, 24, 3; 439-443
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Plasma metabolic differences in cows affected by inactive ovaries or normal ovarian function post partum
    Autorzy:
    Zhao, C.
    Hu, P.
    Bai, Y.L.
    Xia, C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087365.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    dairy cows
    inactive ovaries
    gas chromatography/mass spectrometry
    multivariate statistical analysis
    differential metabolites
    Źródło:
    Polish Journal of Veterinary Sciences; 2020, 23, 1; 59-67
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Serological surveillance of avian influenza virus and canine distemper virus in captive Siberian Tigers in Northeastern China
    Autorzy:
    Wang, K.
    Wang, H.
    Feng, N.
    Zhao, Y.
    Gao, Y.
    Hu, G.
    Xia, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087698.pdf
    Data publikacji:
    2018
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    influenza A virus
    canine distemper virus
    serological
    Siberian Tiger
    Źródło:
    Polish Journal of Veterinary Sciences; 2018, 21, 3; 491-495
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of an indirect ELISA based on glycoprotein B gene for detecting of Feline herpesvirus type 1
    Autorzy:
    Tan, Y.
    Dong, G.
    Niu, J.
    Guo, Y.
    Yi, S.
    Sun, M.
    Wang, K.
    Hu, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087468.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    feline herpesvirus type 1
    gB protein
    indirect ELISA
    prokaryotic expression
    Źródło:
    Polish Journal of Veterinary Sciences; 2019, 3; 631-633
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
    Autorzy:
    Hu, L.
    Lin, X.Y.
    Yang, Z.X.
    Yao, X.P.
    Li, G.L.
    Peng, S.Z.
    Wang, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/30249.pdf
    Data publikacji:
    2015
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
    Źródło:
    Polish Journal of Veterinary Sciences; 2015, 18, 4
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Selection of reference genes for quantitative real-time RT-PCR on gene expression in Golden Pompano (Trachinotus ovatus)
    Autorzy:
    Chen, X.J.
    Sun, Y.
    Zhang, X.Q.
    Huang, S.
    Cao, Z.J.
    Qin, Q.W.
    Hu, W.T.
    Zhou, Y.C.
    Powiązania:
    https://bibliotekanauki.pl/articles/2087908.pdf
    Data publikacji:
    2017
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Źródło:
    Polish Journal of Veterinary Sciences; 2017, 3; 583-594
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus
    Autorzy:
    Lin, A.
    Hu, X.
    Cui, S.
    Yang, T.
    Zhang, Z.
    Li, P.
    Guo, M.
    Lu, Y.
    Powiązania:
    https://bibliotekanauki.pl/articles/16647453.pdf
    Data publikacji:
    2023
    Wydawca:
    Polska Akademia Nauk. Czasopisma i Monografie PAN
    Tematy:
    Getah virus
    real-time PCR
    TaqMan
    detection
    Opis:
    To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
    Źródło:
    Polish Journal of Veterinary Sciences; 2023, 26, 1; 21-28
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
    Autorzy:
    Wu, X.
    Xiao, L.
    Peng, B.
    Wang, Y.
    Yang, Z.
    Yao, X.
    Hu, L.
    Lin, X.
    Powiązania:
    https://bibliotekanauki.pl/articles/30963.pdf
    Data publikacji:
    2016
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Opis:
    African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
    Źródło:
    Polish Journal of Veterinary Sciences; 2016, 19, 1
    1505-1773
    Pojawia się w:
    Polish Journal of Veterinary Sciences
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-12 z 12

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