Autor: Hu, X.L. - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Emerging of canine kobuvirus in dogsin China, 2015
Autorzy:: Liu, D.F.
Liu, X.
Li, Z.J.
F. Liu
Hu, X.L.
Li, Z.G.
Liu, C.G.
Ma, J.Z.
Powiązania:: https://bibliotekanauki.pl/articles/2087863.pdf
Data publikacji:: 2017
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: canine kobuvirus (CaKoV)
dogs
phylogenetic
VP1
China
Źródło:: Polish Journal of Veterinary Sciences; 2017, 4; 707-711
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Prokaryotic expression, purification and antigenicity analysis of African swine fever virus pK205R protein
Autorzy:: Wu, X.
Xiao, L.
Peng, B.
Wang, Y.
Yang, Z.
Yao, X.
Hu, L.
Lin, X.
Powiązania:: https://bibliotekanauki.pl/articles/30963.pdf
Data publikacji:: 2016
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: African swine fever is an acute, febrile and highly virulent porcine disease causing serious economic losses worldwide. The pK205R protein of the African swine fever virus (ASFV) is largely expressed in the early stages of infection, which has given the K205R gene extensive attention. In this study, the ASFV K205R was cloned and expressed in Escherichia coli BL21 (DE3). Expression of histidine-tagged pK205R with a molecular mass of 44 kDa was determined by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. Optimisation of culture conditions allowed induction of the recombinant protein with 0.4 mM Isopropyl β-D-thiogalactoside (IPTG) at 37oC for 2 h. The protein existed in cellular supernatant and was purified using a Ni-NTA resin column. The purified protein was used to immunize rabbits four times to enable the production of polyclonal antibodies, and the antiserum titre was detected by ELISA. The results showed that the purified pK205R can react with ASFV positive serum specifically by Western blotting. The pK205R had high antigenicity, which indicated that pK205R could be used as an antigen for detection of ASFV-specific antibodies in ELISA testing, and the recombinant protein could contribute to further research of the action and structure of pK205R.
Źródło:: Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: A multiplex PCR for simultaneous detection of classical swine fever virus, African swine fever virus, highly pathogenic porcine reproductive and respiratory syndrome virus, porcine reproductive and respiratory syndrome virus and pseudorabies in swines
Autorzy:: Hu, L.
Lin, X.Y.
Yang, Z.X.
Yao, X.P.
Li, G.L.
Peng, S.Z.
Wang, Y.
Powiązania:: https://bibliotekanauki.pl/articles/30249.pdf
Data publikacji:: 2015
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:: In this assay, we developed and evaluated a multiplex PCR (mPCR) for its ability in detecting multiple infections of swine simultaneously. Four pairs of primers were used to detect five viruses. Specific primers were designed for classical swine fever virus (CSFV), African swine fever virus (ASFV) and pseudorabies (PRV). A pair of primers was designed prudently for two different types of porcine reproductive and respiratory syndrome virus that respectively were porcine reproductive and respiratory syndrome virus (PRRSV), highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV). The detection limits of the mPCR were 1.09×10⁴, 1.50×10³, 2.10×10³, 1.30×10³ and 8.97×10² copies/reaction for CSFV, ASFV, HP-PRRSV, PRRSV and PRV, respectively. A total of 49 clinical specimens were tested by the mPCR, and the result showed that co-infection by two or three viruses was 51%. In conclusion, the PCR is a useful tool for clinical diagnosis of not only single infections but also mixed infections in swines.
Źródło:: Polish Journal of Veterinary Sciences; 2015, 18, 4
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 4.

Tytuł:: Detection of IL-1β, IL-6 and TNF-αin Sprague-Dawely rats’ atrophic thymus induced by lipopolysaccharide
Autorzy:: Zhong, Y.B.
Zhang, X.L.
Lv, M.Y.
Hu, X.F.
Li, Y.
Powiązania:: https://bibliotekanauki.pl/articles/2087664.pdf
Data publikacji:: 2018
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: lipopolysaccharide
pro-inflammatory cytokines
thymus atrophy
rat
Źródło:: Polish Journal of Veterinary Sciences; 2018, 21, 3; 589-597
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 5.

Tytuł:: Characterization of serum adiponectin and leptin in healthy perinatal dairy cows or cows with ketosis, and their effectson ketosis involved indices
Autorzy:: Shen, L.
Qian, B.
Xiao, J.
Zhu, Y.
Hussain, S.
Deng, J.
Peng, G.
Zuo, Z.
Zou, L.
Yu, S.
Ma, X.
Zhong, Z.
Ren, Z.
Wang, Y.
Liu, H.
Zhou, Z.
Cai, D.
Hu, Y.
Zong, X.
Cao, S.
Powiązania:: https://bibliotekanauki.pl/articles/2087289.pdf
Data publikacji:: 2020
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: adiponectin
dairy cows
ketosis
leptin
perinatal period
Źródło:: Polish Journal of Veterinary Sciences; 2020, 23, 3; 373-381
1505-1773
Pojawia się w:: Polish Journal of Veterinary Sciences
Dostawca treści:: Biblioteka Nauki

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