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Wyświetlanie 1-15 z 130
Tytuł:
Effect of different egg yolk sources on dog semen quality following cryopreservation
Autorzy:
Strzeżek, R.
Reksa, A.
Powiązania:
https://bibliotekanauki.pl/articles/16539109.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
dog
semen
spermatozoa
extender
egg yolk
Opis:
The aim of this study was to compare the cryoprotective effects of egg yolk from different avian species (hen, goose and quail) on post-thaw quality of dog semen. Total motility (TMOT) and progressive motility (PMOT) of frozen-thawed spermatozoa were not significantly differed among the extenders, but were higher in the quail-egg yolk based extender compared with extender containing hen or goose egg yolk. It was found that post-thaw sperm motion parameters, velocity VCL and ALH, were significantly higher in the quail-egg yolk based extender. No marked differences in post-thaw sperm plasma membrane integrity (PMI) and mitochondrial membrane potential (MMP) were observed among the extenders. In conclusion, the results of the present study suggest that goose or quail egg yolk is a suitable alternative to hen egg yolk for the cryopreservation of dog semen.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 187-189
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Oxidative parameters and expression of 70kDa heat shock proteins in pig heart tissue after transport and slaughter
Autorzy:
Urban-Chmiel, R.
Pyz-Lukasik, R.
Dudzic, A.
Wernicki, A.
Powiązania:
https://bibliotekanauki.pl/articles/31697.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In view of the significant role of Hsp70 in protecting the organism against the destructive effects of stress, and the possibility of using this protein as a marker of the infarction process in the heart, the aim of this study was to conduct an evaluation of the expression of 70kDa heat shock proteins (Hsp70) and the concentration of TBARS (thiobarbituric acid reactive substances) and nitric oxide ions (NO), determined as nitrite ions, as markers of oxidative stress in hearts obtained from healthy pigs following slaughter and pigs which had died during or immediately after transport with symptoms of sudden cardiac death. The material consisted of hearts obtained from 90 pigs following slaughter and from pigs which had died. Oxidative stress was determined in heart lysates based on the concentration of TBARS and nitrite ions. Expression and concentration of Hsp70 were determined using SDS-PAGE, Western blotting, and ELISA. Expression of Hsp70 was observed in hearts lysates obtained from slaughtered pigs and from those which had died with symptoms of sudden death. The strongest reaction in the Western Blotting was noted in hearts lysates from pigs with no pathological changes. The highest TBARS concentration was observed in lysates from hearts in pigs which had died during or immediately after transport. The highest concentration of NO ions, determined as nitrite ions, was noted in hearts from pigs with myocardial infarction lesions. The significant decrease observed in Hsp70 concentration in heart tissue obtained from the pigs which had died in comparison to the hearts from healthy pigs indicates the important role of this protein in protecting the heart muscle against the destructive effects of stress, which limits the occurrence of post-stress cardiomyopathy in pigs following transport.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Factors affecting acetone concentration in blood of dairy cows at the first stage of lactation
Autorzy:
Bialon, K.A.
Skrzypek, R.
Powiązania:
https://bibliotekanauki.pl/articles/30499.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of this paper was to determine effects of selected genetic and environmental factors on the concentration of acetone in blood of cows in the first trimester of lactation. The study included 124 Polish Holstein-Friesian primiparous cows born, reared and managed on the same farm. The samples of blood were collected on day 2, 5, 30, 60 and 90 after calving. The highest serum acetone concentration in the animals examined was observed on day 5 after calving. The level of this compound was inversely related to the contribution of the original Holstein-Friesian breed in gene pool. Significantly higher serum acetone level was observed in cows calved in January-March period when compared to other months of the year. Besides, the level of acetone determined on day 5 after calving was associated positively with body weight determined at the same day and negatively with body weight changes between days 5 and 60 after calving.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 2
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Agreement of different methods for assessment of progesterone concentrations in beef cattle serum
Autorzy:
Rekešiūtė, A.
Januškauskas, A.
Šiukščius, A.
Riškevičienė, V.
Vitkauskienė, A.
Verkauskienė, R.
Antanaitis, R.
Žilinskas, H.
Powiązania:
https://bibliotekanauki.pl/articles/2087384.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
agreement
confidence interval
assessment methods
progesterone
sample extraction
ELISA
RIA
Źródło:
Polish Journal of Veterinary Sciences; 2019, 4; 695-701
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Computed tomographic and densitometric analysis of tibiotarsal bone mineral density and content in postnatal Peking ducks (Anas platyrhynchos var. domestica) as influenced by age and sex
Autorzy:
Charuta, A.
Cooper, R.G.
Powiązania:
https://bibliotekanauki.pl/articles/31058.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
computer tomography
densitometric analysis
tibio-tarsal bone
mineral density
bone density
volumetric density
postnatal period
Pekin duck
Anas platyrhynchos f.domestica
age
sex
Opis:
The bone mineral density BMD and content BMC were analysed in the tibiotarsal bones of ducks in the postnatal development as influenced by age and sex. One hundred birds from the nesting till the slaughtering maturity were included in the experiment. The analysis was conducted using a densitometer, Norland - Excell Plus and pQCT computed tomography, XCT Research SA Plus. The statistical analysis was conducted using the Kruskal-Wallis one-way analysis of variance (age) and the U-Mann -Withney test (sex). All calculations were performed in Statistica 9.0 (StatSoft, Inc. Tulsa, USA), at P ≤ 0.05. Two-factor analysis of variance ANOVA was applied and Pearson’s correlation coefficients were calculated. The densitometer research showed that BMD and BMC increased in the postnatal development for both sexes. The volumetric bone mineral density vBMD analysis using computed tomography showed that volumetric bone mineral density vBMD of the middle of the diaphyses in situ gradually attenuated during the postnatal development both in males and females, i.e. from 620 mg/cm3 (2 wk) to 500 mg/cm3 (8 wk). The biggest vBMD loss was observed in the diaphyses of females in 4 and 6 wk (r = - 0.63 and r = - 0.79; P ≥ 0.05). The BMC decrease was observed in the proximal metaphyses between 4 and 6 wk for both sexes, r = - 0.52 (males), r = - 0.53 (females); P ≤ 0.05. The gradual loss may be the cause of deformities and fractures of the tibiotarsal bones observed from 4 wk in particular bone sections of both sexes. The achieved results may constitute a helpful source of information for water poultry breeders to prevent limb diseases.
Źródło:
Polish Journal of Veterinary Sciences; 2012, 15, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of feeding system and sex on the performance and selected gastrointestinal features of fattening pigs
Autorzy:
Grela, E.R.
Czech, A.
Kusior, G.
Szczotka-Bochniarz, A.
Klebaniuk, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087786.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Feeding system
gender
immunocastration
performance
digestibility
gut microbiology
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 1; 157-165
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phenotypical and genotypical antimicrobial resistance of coagulase-negative staphylococci isolated from cow mastitis
Autorzy:
Klimiene, I.
Virgailis, M.
Pavilonis, A.
Siugzdiniene, R.
Mockeliunas, R.
Ruzauskas, M.
Powiązania:
https://bibliotekanauki.pl/articles/31429.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13.7%). CNS isolates (78.0%) were resistant to at least one antimicrobial compound, and 22.0% were multiresistant. The multiresistant isolates were predominantly Staphylococcus chromogenes (28.6%), Staphylococcus warneri (19%) and Staphylococcus haemolyticus (14.3%). According to MIC pattern data, multiresistant isolates showed the highest resistance (p<0.05) rates to penicillin (85.7%), tetracycline (66.7%), and erythromycin (48.2%), but all of them were sensitive to daptomycin, oxacillin, qiunupristin/dalfopristin, and vancomycin. S. chromogenes (9.5%), S. haemolyticus (4.8%), and S. capitis ss capitis (2.4%) strains were resistant to methicillin; their resistance to oxacillin and penicillin was more than 8 mg/l. A high rate of resistance to penicillin was linked to a blaZ gene found in 66.6% of the isolated multiresistant CNS strains. Resistance to tetracycline via the tetK (38.1%) gene and penicillin via the mecA (23.8%) gene were detected less frequently. Gene msrAB was responsible for macrolides and lincosamides resistance and detected in 28.6% of the CNS isolates. Antimicrobial resistance genes were identified more frequently in S. epidermidis, S. chromogenes, and S. warneri.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
An electrophoretic characterization of iron-transporting proteins in Mannheimia haemolytica A1
Autorzy:
Puchalski, A.
Urban-Chmiel, R.
Dec, M.
Wernicki, A.
Powiązania:
https://bibliotekanauki.pl/articles/30623.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Iron-regulated outer membrane proteins (IROMPs) in Mannheimia haemolytica A1, which function as a receptor for complexes containing iron ions, are induced by iron deficiency in the growth environment of the bacteria. Densitometric analysis of SDS-PAGE separation showed expression of IROMPs of 71, 77, and 100 kDa in the case of bacteria grown in a medium with 2,2-dipyridyl. The electrophoregrams obtained in 2-DE separations confirmed the presence of protein fractions with these molecular weights and isoelectric points ranging from 5.4 to 6.4. The results of the study also confirmed the ability of M. haemolytica A1 proteins involved in iron uptake to induce a protective immune response. In Western blot with serum from convalescent calves naturally infected with M. haemolytica A1, distinct reactions were obtained for IROMPs of 71, 77, and 100 kDa.
Źródło:
Polish Journal of Veterinary Sciences; 2013, 16, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Assessment of biofilm formation by faecal strains of Enterococcus hirae from different species of animals
Autorzy:
Bino, E.
Lauková, A.
Kandričáková, A.
Nemcová, R.
Powiązania:
https://bibliotekanauki.pl/articles/2087614.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Enterococcus hirae
various animals
biofilm
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 4; 747-754
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Mapping of polar fox renal cortex proteins using two-dimensional gel electrophoresis and mass spectrometry – a preliminary study
Autorzy:
Ciechanowicz, A.K.
Ozgo, M.
Stanski, L.R.
Herosimczyk, A.
Piotrowska, A.
Szymeczko, R.
Laszczynska, M.
Skrzypczak, W.F.
Powiązania:
https://bibliotekanauki.pl/articles/30461.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to establish protein map of polar fox (Alopex lagopus) renal cortex. Kidney cortex proteins of isoelectric point ranging from 3 to 10 were analysed using two-dimensional electrophoresis and MALDI-TOF mass spectrometry. Sixteen protein spots corresponding to thirteen different gene products were identified. These proteins were divided into following groups: lipid and fatty acid metabolism, amino acid metabolism, energetic pathways, regulatory proteins, transport proteins and structural proteins. This is the first attempt to create reproducible 2-D map, of renal cortex proteins characteristic for polar foxes, used as animal model for carnivores. It is worth emphasizing that the results of this study may broaden currently available protein databases.
Źródło:
Polish Journal of Veterinary Sciences; 2014, 17, 2; 231-237
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A novel orchiectomy surgical procedure in donkeys (Equus asinus africanus) with parascrotal access
Autorzy:
Barrêto JR, R.A.
Rodrigues, L.A.
Albuquerque, J.P.
de Sousa, F.J.A.
Firmino, P.R.
Sousa, R.S.
Pedrosa, V.J.
do Amaral, T.R.
Minervino, A.H.H.
Powiązania:
https://bibliotekanauki.pl/articles/16539181.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
inflammation
donkeys
peritoneal fluid
surgical approach
castration
Opis:
Donkeys are a public health concern in the Northeast region of Brazil, with thousands of stray animals. Orchiectomy is an important population control measure; however, the long postoperative period with daily treatment of open wounds in the scrotum makes it difficult to perform a large number of castrations in sheltering centers. We evaluate a novel surgical procedure for orchiectomy in donkeys using parascrotal access. Twelve donkeys were used, divided into two groups: I - submitted to orchiectomy through parascrotal surgical access (novel procedure), and II - submitted to orchiectomy through scrotal access (conventional). Postoperative evaluations consisted of a macroscopic evaluation of the surgical wound (bleeding and intensity of edema), hematological parameters, and peritoneal fluid, which occurred in both groups at the moments (M): M0 - before the surgical procedure. The others moments occurred after surgery: M12 (twelve hours); M24 (twenty-four hours); M48 (forty-eight hours); M72 (seventy-two hours); M8D (eight days); and M16D (sixteen days). The surgical techniques did not generate an important systemic inflammatory response to the point detected by the leukogram, fibrinogen dosage, and peritoneal fluid. The parascrotal technique required long surgery but promoted less bleeding, less edema, and faster healing. The techniques used did not promote sufficient systemic inflammation to alter the number of leukocytes and the fibrinogen concentration; however, evaluation of the peritoneal fluid proved to be important for evaluating inflammatory processes involving the scrotum and inguinal canal. We describe a novel surgical procedure for orchiectomy in Donkeys using a parascrotal access that promoted less risk of bleeding, shorter period of edema, and healing time, but required longer surgery time.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 2; 295-302
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Milk production and ruminal parameters of dairy cows fed diets containing Lactobacillus sakei KTU05-6 and Pediococcus pentosaceus BaltBio02
Autorzy:
Lele, V.
Zelvyte, R.
Monkeviciene, I.
Kantautaite, J.
Stankevicius, R.
Ruzauskas, M.
Sederevicius, A.
Antanaitis, R.
Bartkiene, E.
Powiązania:
https://bibliotekanauki.pl/articles/2087548.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
dairy cows
lactic acid bacteria
antimicrobial activities
rumen
milk
Źródło:
Polish Journal of Veterinary Sciences; 2019, 2; 327-335
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Phylogenetic characterization of Canine Parvovirus VP2 partial sequences from symptomatic dogs samples
Autorzy:
Zienius, D.
Lelesius, R.
Kovaliauskis, H.
Stankevicius, A.
Salomskas, A.
Powiązania:
https://bibliotekanauki.pl/articles/31615.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The aim of the present study was to detect canine parvovirus (CPV) from faecal samples of clinically ill domestic dogs by polymerase chain reaction (PCR) followed by VP2 gene partial sequencing and molecular characterization of circulating strains in Lithuania. Eleven clinically and antigen-tested positive dog faecal samples, collected during the period of 2014-2015, were investigated by using PCR. The phylogenetic investigations indicated that the Lithuanian CPV VP2 partial sequences (3025-3706 cds) were closely related and showed 99.0-99.9% identity. All Lithuanian sequences were associated with one phylogroup, but grouped in different clusters. Ten of investigated Lithuanian CPV VP2 sequences were closely associated with CPV 2a antigenic variant (99.4% nt identity). Five CPV VP2 sequences from Lithuania were related to CPV-2a, but were rather divergent (6.8 nt differences). Only one CPV VP2 sequence from Lithuania was associated (99.3% nt identity) with CPV-2b VP2 sequences from France, Italy, USA and Korea. The four of eleven investigated Lithuanian dogs with CPV infection symptoms were vaccinated with CPV-2 vaccine, but their VP2 sequences were phylogenetically distantly associated with CPV vaccine strains VP2 sequences (11.5-15.8 nt differences). Ten Lithuanian CPV VP2 sequences had monophyletic relations among the close geographically associated samples, but five of them were rather divergent (1.0% less sequence similarity). The one Lithuanian CPV VP2 sequence was closely related with CPV-2b antigenic variant. All the Lithuanian CPV VP2 partial sequences were conservative and phylogenetically low associated with most commonly used CPV vaccine strains.
Źródło:
Polish Journal of Veterinary Sciences; 2016, 19, 1
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A comparison of the effectiveness of the microscopic method and the multiplex PCR method in identifying and discriminating the species of Nosema spp. spores in worker bees (Apis mellifera)from winter hive debris
Autorzy:
Michalczyk, M.
Sokol, R.
Szczerba-Turek, A.
Bancerz-Kisiel, A.
Powiązania:
https://bibliotekanauki.pl/articles/32256.pdf
Data publikacji:
2011
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The objective of this study was to compare the effectiveness of the multiplex PCR method and traditional light microscopy in identifying and discriminating the species of Nosema spp. spores in worker bees from winter hive debris in the Province of Warmia and Mazury (NE Poland). A total of 1000 beesdead after from the bottom of the hive from bee colonies were analyzed. Spores were identified with the use of a light microscope (400-600x magnification). Spores were assigned to species by the multiplex PCR method. The microscopic evaluation revealed the presence of Nosema spp. spores in 803 samples (80.3%). Nosema ceranae spores were observed in 353 positive samples (43.96%), Nosema apis spores were found in 300 samples (37.35%), while 150 samples (19.67%) showed signs of a mixed infection. A multiplex PCR analysis revealed that 806 samples were infested with Nosema spp., of which 206 were affected only by Nosema ceranae, 600 showed signs of mixed invasion, while no samples were infected solely by Nosema apis parasites. In two cases, the presence of spores detected under a light microscope was not confirmed by the PCR analysis. The results of the study indicate that Nosema ceranae is the predominant parasitic species found in post-winter worker bees from the bottom of the hive in the region of Warmia and Mazury.
Źródło:
Polish Journal of Veterinary Sciences; 2011, 14, 3
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of rumination time, subsequent yield, and milk trait changes dependenton the period of lactation and reproductive status of dairy cows
Autorzy:
Antanaitis, R.
Žilaitis, V.
Juozaitienė, V.
Noreika, A.
Rutkauskas, A.
Powiązania:
https://bibliotekanauki.pl/articles/2087676.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cow
rumination
milk yield
reproduction
Nauki Biologiczne i Rolnicze
Źródło:
Polish Journal of Veterinary Sciences; 2018, 21, 3; 567-572
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 130

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