Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Wyszukujesz frazę "M.H." wg kryterium: Autor


Tytuł:
Effects of low ozone concentrations and short exposure times on the mortality of immature stages of the Indian meal moth, Plodiainterpunctella (Lepidoptera: Pyralidae)
Autorzy:
Keivanloo, E.
Namaghi, H.S.
Khodaparast, M.H.H.
Powiązania:
https://bibliotekanauki.pl/articles/66028.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
In Iran, the Indian meal moth, Plodia interpunctella (Hübner), is one of the most important pests of such stored products as date fruits and pistachio nuts. Ozone was applied as a gas at four concentrations (0, 2, 3, and 5 ppm) for four different periods (30, 60, 90, and 120 min) on the immature stages of P. interpunctella. The results indicated that by increasing the concentration and exposure time, the rate of mortality increased for all tested stages. This study showed that 12-day-old larvae were more susceptible than other stages when exposed to 5 ppm ozone for 120 min. The next in order of susceptibility were pupae, then 5-day-old larvae, and 17-dayold larvae had the highest sensitivity to ozonation. At the highest concentration of ozone, for the longest time, the least mortality rate was recorded for one-day-old eggs. According to the results, a reduction in the population density of P. interpunctella in laboratory experiments is promising. However, validation studies will be necessary to fully determine the potential of ozone as a replacement for the current post harvest chemical control of P. interpunctella on either pistachio nuts or date fruits.
Źródło:
Journal of Plant Protection Research; 2014, 54, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The effect of Beauveria bassiana on Brazilian poplar moth Condylorrhiza vestigialis (Lepidoptera: Crambidae)
Autorzy:
Pogetto, M.H.F.
Wilcken, C.F.
Powiązania:
https://bibliotekanauki.pl/articles/66450.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The Brazilian poplar moth is the most important pest of poplar plantations in Brazil. This research evaluated the effect of Beauveria bassiana Bals. (Vuill.) on the mortality and development of Condylorrhiza vestigialis Guen. (Lepidoptera: Crambidae). The aim was to develop alternative methods for management of this pest. The pathogens were sprayed on poplar leaves and consequently, the pathogens reached the caterpillars. Bacillus thuringiensis var. kurstaki Berliner was sprayed as the standard treatment. The spray for the control was distilled water. Both pathogens B. bassiana and B. thuringiensis affected insect development with increase mortality at each stage of the insect cycle, reaching a satisfactory control level. Microbial control of Brazilian poplar moth with B. bassiana is promising. Tests with other strains and species of pathogens, mainly under field conditions, were also encouraging. This is the first report about the action of B. bassiana against C. vestigialis.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of a new chemical formula on postharvest decay incidence in citrus fruit
Autorzy:
El-Mougy, N.S.
Abdel-Kader, M.M.
Aly, M.H.
Powiązania:
https://bibliotekanauki.pl/articles/66793.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Postharvest diseases caused by Geotricum candidum (sour rot), Penicillium digitatum (green mould), and P. italicum (blue mould) are the most important negative factors affecting handling and marketing of citrus fruits in Egypt. A new formula containing stevia leaf powder and a mixture of the three commercial chemical active ingredients: ketoconazole, fluconazole, and itraconazole has been successfully applied. Either chitosan or water wax were used as carriers, against fruit mould pathogenic fungi under laboratory and storage conditions. Results of the in vitro test showed that a complete reduction in linear fugal growth was observed when the ingredients of the new formula were used individually at a concentration of 400 μg/ml each, while a mixture of all the tested chemicals had a superior effect with all fungal growth completely inhibited with the use of the mixture at a concentration of 100 μg/ml. Similar results were recorded on citrus fruits which were coated with the suggested formula of chitosan or wax containing chemical compounds as a semi applicable technique using navel orange peel discs. Furthermore, the obtained results were confirmed using in vivo testing on navel orange and lime fruits, under artificial inoculation conditions of the pathogenic fungi within a storage period extended for four weeks. The proposed approach provides the treated agricultural products with long acting protection against microbial invasion and even association. This formula could be used as a fungicide alternative for protecting the agricultural products which have high moisture contents. The formula can be used against mould pathogens to prolong the healthy shelf life of the agricultural products. Such a treatment is safe, cheap, easily applied, and without residues which are harmful to people and the environment.
Źródło:
Journal of Plant Protection Research; 2012, 52, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Detection of significant wavelengths for identifying and classifying Fusarium oxysporum during the incubation period and water stress in Solanum lycopersicum plants using reflectance spectroscopy
Autorzy:
Ortiz, J.C.M.
Carvajal, L.M.H.
Fernandez, V.B.
Powiązania:
https://bibliotekanauki.pl/articles/65540.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Spectroscopy has become one of the most used non-invasive methods to detect plant diseases before symptoms are visible. In this study it was possible to characterize the spectral variation in leaves of Solanum lycopersicum L. infected with Fusarium oxysporum during the incubation period. It was also possible to identify the relevant specific wavelengths in the range of 380–1000 nm that can be used as spectral signatures for the detection and discrimination of vascular wilt in S. lycopersicum. It was observed that inoculated tomato plants increased their reflectance in the visible range (Vis) and decreased slowly in the near infrared range (NIR) measured during incubation, showing marked differences with plants subjected to water stress in the Vis/NIR. Additionally, three ranges were found in the spectrum related to infection by F. oxysporum (510–520 nm, 650–670 nm, 700–750 nm). Linear discriminant models on spectral reflectance data were able to differentiate between tomato varieties inoculated with F. oxysporum from healthy ones with accuracies higher than 70% 9 days after inoculation. The results showed the potential of reflectance spectroscopy to discriminate plants inoculated with F. oxysporum from healthy ones as well as those subjected to water stress in the incubation period of the disease.
Źródło:
Journal of Plant Protection Research; 2019, 59, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Weed hosts of root - knot nematodes in tomato fields
Autorzy:
Gharabadiyan, F.
Jamali, S.
Yazdi, A.A.
Hadizadeh, M.H.
Eskandari, A.
Powiązania:
https://bibliotekanauki.pl/articles/66816.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
Root-knot nematodes (Meloidogyne spp.) are one of the three most economically damaging genera of plant parasitic nematodes on horticultural and field crops. Root-knot nematodes are distributed worldwide, and are obligate parasites of the roots of thousands of plant species. All major field crops, vegetable crops, turf, ornamentals, legumes and weeds are susceptible to one or more of the root-knot species. In this study, nineteen weed species were found to be hosts for Meloidogyne incognita, M. javanica, M. arenaria race 2, and M. hapla in tomato fields in Khorasan Province, Iran. Egg mass production and galling differed (p < 0.05) among these weed species: Amaranthus blitoides, Portulaca oleracea, Polygonum aviculare, Convolvulus arvensis, Cyperus rotundus, Plantago lanceolatum, Rumex acetosa, Solanum nigrum, Datura stramonium, Acroptilon repens, Alcea rosa, Alhaji camelorum, Chenopodium album, Echinochla crusgalli, Hibiscus trionum, Kochia scoparia, Malva rotundifolia, Setaria viridis, Lactuca serriola. The species P. oleracea, A. blioides, S. nigrum, P. lanceolatum, Ch. album, and C. arvensis are major threats to the natural ecosystem in the Iranian province of Khorasan. A. blitoides collected from tomato fields was a good host for 4 Meloidogyne species. C. arvensis, as an important weed, was a distinguished appropriate host for M. hapla, M. incognita, M. javanica. S. nigrum and Ch. album were good hosts for M. hapla, M. javanica, M. incognita race 1, and M. arenaria race 3. In this survey, we reported E. crusgalli as a new host of M. javanica and C. rotundus was a good host for M. arenaria and M. incognita. S. nigrum was also reported as a new host of M. hapla. R. acetosella was reported as a host of M. arenaria. M. incognita was recently described as a new species infecting D. stramonium worldwide.
Źródło:
Journal of Plant Protection Research; 2012, 52, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Air-assistance in spray booms which have different spray volumes and nozzle types for chemically controlling Spodoptera frugiperda on corn
Autorzy:
Gimenes, M.J
Raetano, C.G.
Ferreira, M.H
Pereira-Prado, E.
Souza-Christovam, R.
Tourino-Rezende, D.
Almeida-Costa, S.I.
Powiązania:
https://bibliotekanauki.pl/articles/66410.pdf
Data publikacji:
2012
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The study aimed to evaluate the performance of air assistance in spray booms using different types of nozzles and spray volumes. We took into account spray deposits, fall armyworm control and crop corn performance in a narrow row cropping system. The experiment was carried out at the experimental area of Sao Paulo State University, Botucatu/SP, Brazil, during the 2008/2009 agricultural season, in randomized blocks with a factorial scheme (2×2+1) and four replications. Two spray nozzles (flat fan nozzle and hollow cone nozzle) were tested, combined with two air assistance levels in the spray boom (with and without air assistance) and a treatment control. In the experimental spraying, Spinosad insecticide was sprayed in amounts of 48 g active substance (a.s.)/ha. The air assistance in the spray boom increased the spray deposits in the V4 growth stage of the corn plants. Moreover, the application of this technology showed higher efficiency on fall armyworm control, reaching a 100% level 15 days after spraying, in the V10 growth stage of the plants. The hollow cone nozzle increased the spray deposit level on the corn plants compared with the flat fan nozzle, at growth stage V4. However, the flat fan nozzle, combined with air assistance technology, was more effective for controlling fall armyworm in the same growth stage (V4), although the hollow cone nozzle increased the deposit levels on the plants. All the technologies tested in the study promoted a reduction of plant damage from fall armyworm attack. Corn productivity is directly related to the control efficiency of fall armyworm.
Źródło:
Journal of Plant Protection Research; 2012, 52, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Purification and characterization of the cuticle-degrading proteases produced by an isolate of Beauveria bassiana using the cuticle of the predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae)
Autorzy:
Firouzbakht, H.
Zibaee, A.
Hoda, H.
Sohani, M.M.
Powiązania:
https://bibliotekanauki.pl/articles/65065.pdf
Data publikacji:
2015
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
The entomopathogenic fungi-like Beauveria bassiana must penetrate via the integument of an insect to reach the hemocoel. Since proteins are the molecules responsible for integument strength in insects, the proteins must synthesise the cuticle degrading proteases which will then enable the proteases to penetrate. It is important to determine the biochemical properties of these proteases so that fungal virulence can be better understood. In the current study, a recently collected isolate of B. bassiana, namely AM-118, was inoculated in liquid media containing 0.5% of Andrallus spinidens Fabricus cuticle to obtain specific proteases. The crude samples were purified via a three step process using ammonium sulfate, Sepharyl G-100, and DEAE-Cellulose Fast Flow. The results revealed two proteases known as subtilisin-like (Pr1), and trypsin-like (Pr2), with the molecular weights of 105 and 103 kDa. The optimal pH and temperature values were found to be 8 and 35°C for Pr1 and 8 and 40°C for Pr2, respectively. Inhibitors like AEBSF, EDTA, TPCK, and phenanthroline significantly affected proteolytic activities. Here, we reported two fungal proteases by high molecular weight from an Iranian isolate of B. bassiana. These findings will help us to better understand fungal virulence against insects.
Źródło:
Journal of Plant Protection Research; 2015, 55, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Marker-assisted selection for scald (Rhynchosporium commune L.) resistance gene(s) in barley breeding for dry areas
Autorzy:
Sayed, H.
Baum, M.
Powiązania:
https://bibliotekanauki.pl/articles/65161.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
marker-assisted selection
marker aided selection zob.marker-assisted selection
leaf blotch
scald zob.leaf blotch
Rhynchosporium commune
resistance gene
barley
plant breeding
dry climate
foliar disease
plant disease
fungal disease
Opis:
Barley scald, caused by Rhynchosporium commune is one of the most prevalent diseases in barley (Hordeum vulgare L.) worldwide. The primary loss from scald is reduced yield, which can exceed 25% in dry areas. In our earlier studies, we developed a low-resolution linkage map for recombinant inbred lines of the cross Tadmor/WI2291. Quantitative trait loci (QTLs) for scald were localized on chromosomes 2H and 3H flanked by Simple Sequence Repeat (SSR) markers HVM54 and Bmac0093b on 2H and HVLTPP8, HVM62 and Bmag0006 on 3H. These chromosome 3H markers were found to be located close to the Rrs1 − R. commune resistance gene(s) on chromosome 3H. In this study, 10 homozygous resistant and 10 homozygous susceptible plants each from the F7 population of Tadmor/ Sel160, a panel of 23 barley varieties used routinely in the International Centre for Agricultural Research in the Dry Areas (ICARDA) breeding program and three populations were used for scald resistance screening using 25 DNA markers that are located very close to scald resistance gene(s) on barley chromosomes. Only five of those markers clearly discriminated co-dominantly between resistant and susceptible plants. These markers, Ebmac0871- SSR, HVS3-SCAR, Bmag0006-SSR, reside on different arms of barley chromosome 3H. Ebmac871 is localized on the short arm of 3H and HVS3 and Bmag0006 are localized on the long arm of 3H. This result indicates that the scald resistance genes which they tag are probably close to the centromeric region of this chromosome. Scald resistance from several sources map to the proximal region of the long arm of chromosome 3H, forming the complex Rrs1 locus. The availability of highly polymorphic markers for the discrimination of breeding material would be extremely useful for barley breeders to select for the trait at the DNA level rather than relying on phenotypic expression and infection reaction.
Źródło:
Journal of Plant Protection Research; 2018, 58, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The occurrence of Septoria tritici and its teleomorph Mycosphaerella graminicola in the Region of Upper Silesia in 1996
Autorzy:
Glazek, M
Sikora, H.
Powiązania:
https://bibliotekanauki.pl/articles/66312.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Upper Silesian region
plant disease
Septoria tritici
wheat
Polska
morphology
occurrence
leaf
winter wheat
Mycosphaerella graminicola
Opis:
The occurrence of leaf blotch of wheat caused by Septoria tritici (teleomorph Mycosphaerella graminicola) was more frequent in 1996 in the Upper Silesia region, south-western Poland, than in previous years. Visual scoring and serological tests proved the most rapid development of the disease from the emergence of flag leaf (GS 49) onwards. At the end of dough ripe stage (GS 85) on necrotic spots appearing on leaves the teleomorph of the fungus was stated, although asci were not fully mature. After a period of storage, on the necrotic tissue with pseudothecia, placed on water agar-streptomycin and subjected to low temperatures for 2-3 weeks, mature asci and germinating ascospores forming single conidia on ramifying mycelium were abundant.
Występowanie plamistości liści powodowanych przez Septoria tritici (teleomorf Mycosphaerella graminicola) było częściej spotykane w 1996 roku niż w latach poprzednich w Rejonie Górnego Śląska. Wizualne oceny i test serologiczny wykazały najszybszy rozwój choroby od fazy rozwiniętego liścia flagowego (GS 49) i później. W końcu fazy dojrzałości ciastowatej pszenicy (GS 85) na znekrotyzowanych plamach stwierdzono teleomorf grzyba Septoria tritici, jednakże worki nie były w pełni dojrzałe. Po okresie przechowywania znekrotyzowane tkanki z pseudotecjami umieszczano na wodnym agarze ze streptomycyną i poddawano niskiej temperaturze przez 2-3 tygodnie. Obserwowano liczne dojrzałe worki i kiełkujące askospory formujące pojedyncze konidia na rozgałęzionej grzybni.
Źródło:
Journal of Plant Protection Research; 1998, 38, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Acaricidal, ovicidal, and repellent effects of Tagetes patula leaf extract against Tetranychus urticae Koch (Acari: Tetranychidae)
Autorzy:
Ismail, M.S.M.
Tag, H.M.
Rizk, M.A.
Powiązania:
https://bibliotekanauki.pl/articles/65234.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
This study investigates the acaricidal, ovicidal, and repellent effects of the Tagetes patula Linn. (Asteraceae) leaf extract against both the adult female and egg stages of Tetranychus urticae Koch (Trombidiformes: Tetranychidae) under laboratory conditions. The Tagetes patula ethanolic leaf extract [TpEtOH70%] was screened for adulticide and ovicide bioassays in order to consider its acute toxicity. One sublethal concentration was used to assess egg-laying capacity (fecundity), repellent, and oviposition deterrent activities. The chemical characterization was conducted by gas chromatography-mass spectrometry (GC-MS) analysis to identify the TpEtOH70% bioactive components. Results showed that the LC50 value of TpEtOH70% leaf extract predicted by Probit analysis against T. urticae adult females at 24 h was 0.99%.The TpEtOH70% leaf extract showed a significant toxic effect as the highest mean mortality rates (± SE) of the treated adult females was 88.9 ± 3.7%. However, the TpEtOH70% leaf extract was insignificant in affecting the egg-laying capacity of the adult females treated with a sublethal dose of 0.5% even after 72 h. The TpEtOH70% leaf extract was classified repellent since the repellent index (RI) value was lower than 1 – SD. In addition, it had a high oviposition deterring effect based on a 100% reduction of the total number of eggs. The TpEtOH70% leaf extract had a significant ovicidal effect on T. urticae eggs, with 56.04% reduction in hatching. Five bioactive compounds from various classes of phytochemicals were identified in the TpEtOH70% leaf extract and the major compound was phytol (62.72%). This pioneering investigation reveals the adulticidal, ovicidal, and repellent activities of the TpEtOH70% leaf extract against T. urticae. A combination of multiple modes of action of different plant components may act alone or in synergism to delay the development of mite resistance.
Źródło:
Journal of Plant Protection Research; 2019, 59, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Chemical constituents and ovicidal effects of mahlab, Prunus mahaleb L. kernels oil on cotton leafworm, Spodoptera littoralis (Boisd.) eggs
Autorzy:
Mead, H.M.
El-Shafiey, S.N.
Sabry, H.M.
Powiązania:
https://bibliotekanauki.pl/articles/65600.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
cotton leafworm
chemical constituent
enzyme
mahlab zob.mahleb
mahleb
spice
Prunus mahaleb
oil
Spodoptera littoralis
egg
Opis:
The carried out investigations evaluated ovicidal activity of mahlab, Prunus mahaleb L. kernel oil against cotton leafworm, Spodoptera littoralis (Boisd.). The chemical constituents of the fixed oil of mahlab were analyzed using gas-liquid chromatography (GLC). Timnodonic (33.07%), oleic (28.71%) and linoleic (24.35%) were the basic fatty acids, while the major hydrocarbon and sterol were found to be heneicosane (62.57%) and β-sitosterol (10.57%). The LC50 values for the one-day-old egg masses were found to be more susceptible than 3-day-old ones. Moreover, the leaf dip technique occurred to be more efficient than spraying technique. The results also showed abnormalities in the external morphology of egg shell, chorion surface, shell imprints and aeropyles of S. littoralis eggs treated with mahlab and KZ oils as compared to a control by using scanning electron microscope. Generally, the tested oils significantly reduced the activities of transaminase enzymes (AST and ALT), acid and alkaline phosphatases and total soluble protein except mahlab oil on acid phosphatase as compared to a control. Additionally, the oils of both mahlab and KZ oil affected some biological aspects such as incubation period, larval duration, larval mortality and pupal weight comparing to a control.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of amino acid application on induced resistance against citrus canker disease in lime plants
Autorzy:
Hasabi, V.
Askari, H.
Alavi, S.M.
Zamanizadeh, H.
Powiązania:
https://bibliotekanauki.pl/articles/65104.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
amino acid
citrus bacterial canker
canker
plant disease
lime
induced resistance
Opis:
Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc), is a destructive disease. So far, the chemicals used to control this pathogen are either ineffective or harmful to the environment. To improve control of this disease, lime (Citrus aurantifolia) were treated with L-arginine, L-methionine, L-ornithine, and distilled water. Plants were inoculated with Xcc, 48 hours post treatment. Lesion diameters of inoculated leaves were evaluated four weeks after inoculation with a bacterial suspension. Changes in β-1,3-glucanase transcript levels and activity of antioxidant enzymes, catalase, peroxidase, and phenylalanine ammonia-lyase were investigated at 48 hours post treatment and 24, 48, and 72 hours post inoculation. Based on the results of phenotypic, antioxidant enzyme activity and a molecular study of the stressed plants, it was found that those plants treated with the amino acid methionine significantly increased the plant induced resistance as well as decreased the severity of disease by reducing necrotic lesion size.
Źródło:
Journal of Plant Protection Research; 2014, 54, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Agar-agar a promising edible coating agent for management of postharvest diseases and improving banana fruit quality
Autorzy:
Ziedan, E.S.H.
El Zahaby, H.M.
Maswada, H.F.
Zoeir, H.A.
Powiązania:
https://bibliotekanauki.pl/articles/66110.pdf
Data publikacji:
2018
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
banana
Musa
fruit
storage
fruit disease
fungi
agar
edible coating
postharvest disease
Opis:
This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable gelling agent, as a promising coating agent to improve and protect banana fruit against fungal postharvest diseases i.e., crown, finger, neck and flower end rots which are caused by fungal isolates of Colletotrichum musae and Fusarium moniliforme. Coated-banana fruit samples with different concentrations of agar-agar suspension particularly at 2.0 g · l−1 exhibited a significant reduction in incidence and severity of postharvest diseases compared to untreated fruit. Banana fruits dipped in agar suspension at 2.0 g · l−1 for 5, 10 and 15 min showed significant reduction in disease incidence and severity. Moreover, application of agar suspension as a coating agent at 2.0 g · l−1 significantly decreased weight loss (%), firmness loss (%), and soluble solid concentration of banana fruit for 15 days at 25 ± 2°C. Scanning electron microscopy observation confirmed that the fruit coated with agar colloid at 2.0 g · l−1 had significantly fewer cracks and showed smoother surfaces than untreated fruit. This explains the quality improvement in agar-coated fruit compared to uncoated fruit. Overall, agar colloid, a safe coating agent, could be used to protect banana fruit against postharvest rot diseases and extend fruit storage life during ripening and storage.
Źródło:
Journal of Plant Protection Research; 2018, 58, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The invasive flea beetle Luperomorpha xanthodera (Coleoptera: Chrysomelidae: Alticinae), potentially noxious to ornamental plants - first record in Poland
Autorzy:
Kozlowski, M.W.
Legutowska, H.
Powiązania:
https://bibliotekanauki.pl/articles/65358.pdf
Data publikacji:
2014
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Opis:
A specimen of a flea beetle collected by plant sweeping from an experimental plot of oregano in Warsaw showed characteristics of the invasive species Luperomorpha xanthodera. This beetle, originating from Far East, appeared in Europe at the beginning of this century and seems to extend its range continuously. So far, it has been noted from England, Italy, France, The Netherlands, Germany, Austria and Hungary but not from Poland. Being polyphagous, adult flea beetles of this species may appear in numbers on flowers of various ornamental plants causing considerable damage.
Źródło:
Journal of Plant Protection Research; 2014, 54, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The use of reverse transcription and polymerase chain reaction [RT-PCR] for the detection of hop latent viroid [HLVd]
Wykrywanie wiroida latentnego chmielu [HLVd] przy pomocy odwrotnej transkrypcji reakcji lancuchowej polimerazy [RT-PCR]
Autorzy:
Cajza, M
Wypijewski, K.
Pospieszny, H.
Powiązania:
https://bibliotekanauki.pl/articles/65322.pdf
Data publikacji:
1997
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
patogeny roslin
lancuchowa reakcja polimerazy
wiroid latentny chmielu
wiroidy
odwrotna transkrypcja
wykrywanie
Opis:
The simple method of nucleic acids extraction, based on guanidine thiocyanate extraction buffer, was sufficient for obtaining a good templates for RT-PCR. The RT-PCR reactions were performer from the start to the end (both the reverse transcription and the HLVd-cDNA amplification) in the same reaction mixture and in the very small volume of reaction (10 μI). Both pairs of primers designed by authors were good for reverse transcription and later for amplification of the HLVd-cDNA. The presence of gelatin as a stabilizer of DNA polymerase was indispensable for successful performance of RT-PCR.
Wiroidy, najmniejsze obecnie znane patogeny roślin, zbudowane z pojedynczej nici kolistego RNA, nie zawierające w swej budowie i nie kodujące żadnych białek, są groźnymi patogenami roślin wyższych, rozpowszechnionymi we wszystkich rejonach świata, szczególnie w uprawach roślin rozmnażanych wegetatywnie. Wiroid latentny chmielu (ang. hop latent viroid - HLVd) został wykryty dopiero w 1988 roku w chmielu. Do tej pory odnotowano go w rejonach uprawy chmielu na całym świecie. Chmiel, jedyny znany żywiciel tego patogena, ulega tylko infekcjom utajonym (latentnym). HLVd powoduje zarówno spadek masy plonu szyszek jak i zawartości alfa-kwasów w szyszkach. Bezobjawowe infekcje oraz brak białek strukturalnych i innych produktów translacji powodują, że obecnie patogena tego można wykrywać tylko przy pomocy elektroforezy (metoda bardzo zawodna, wymagająca wysokiego stężenia RNA patogena w tkance), sond hybrydyzacyjnych i rozwijanej w ostatnich latach metody RT-PCR. Podczas opracowywania RT-PCR do wykrywania HLVd w ekstraktach kwasów nukleinowych wykorzystywano dwie pary primerów specyficznych dla sekwencji nukleotydowej HLVd, charakteryzujących się różnymi temperaturami topnienia produktu. Wiroida wykrywano w ekstraktach kwasów nukleinowych z blaszek liściowych i z ogonków liściowych chmielu. Uproszczona metoda guanidynowa do izolacji totalnych kwasów nukleinowych okazała się wystarczająca do przeprowadzenia reakcji RT-PCR. Opracowana metoda charakteryzowała się bardzo dużą czułością, specyficznością (produkty amplifikacji cDNA-HLVd uzyskiwano tylko z próbek materiału roślinnego pochodzącego z roślin zawierających tego wiroida) i szybkością wykonania (dwa dni łącznie z ekstrakcją kwasów nukleinowych). Ponadto w zastosowanej metodzie opracowano warunki do przeprowadzenia zarówno odwrotnej transkrypcji i następnie amplifikacji powstałego cDNA wiroida w jednej probówce, w tej samej mieszaninie reakcyjnej . Oprócz tego wszystkie reakcje RT-PCR prowadzono w bardzo małej objętości równej 10 μl (2 μl zawiesiny kwasów nukleinowych i 8 μl mieszaniny reakcji RT-PCR). Maksymalne uproszczenie metody, wielokrotne obniżenie kosztów reakcji oraz charakterystyczna dla RT-PCR czułość, specyficzność i szybkość przeprowadzenia testu sprawiają, że może być ona wykorzystywana do rutynowego wykrywania nie tylko HLVd ale również innych wiroidów, wirusoidów i wirusów RNA.
Źródło:
Journal of Plant Protection Research; 1997, 37, 1-2; 52-58
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies