Temat: somatic - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Effect of genotype and media composition on embryoid induction and plant regeneration from anther culture in triticale
Autorzy:: Slusarkiewicz-Jarzina, A
Ponitka, A
Powiązania:: https://bibliotekanauki.pl/articles/2046679.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: plant regeneration
microspore
triticale
haploid plant
anther culture
embryonic structure
androgenesis
somatic embryo
Opis:: Anthers of twenty triticale genotypes were cultured on three different media: 1 - PII (Chuang et al. 1978) with increased 2,4-D to 2 mg L⁻¹ and agarose 6 g L⁻¹ , 2 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 90 g L⁻¹ sucrose; 3 - Macro-, micronutrients and vitamines like in MN6 (Chu, Hill, 1988) + 2 mg L⁻¹ 2.4-D + 0.5 mg L⁻¹ KIN + 5 mg L⁻¹ FeEDTA + 120 g L⁻¹ sucrose. Embryoid induction and plant regeneration were influenced by donor plant genotype and induction medium. Medium 1 was the best for embryoid induction, while for green plant regeneration the best were media 1 and 2. Out of 300 anthers from each genotype plated on each of the three media, 64-1250, 12-486 and 6-212 somatic embryos and 8-86, 3-136 and 1-26 green plants were recorded, on media 1, 2 and 3, respectively.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 253-258
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 2.

Tytuł:: Rice improvement, involving altered flower structure more suitable to cross-pollination, using in vitro culture in combination with mutagenesis
Autorzy:: Min, S K
Powiązania:: https://bibliotekanauki.pl/articles/2048292.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: japonica rice
radiation
China
mutagenesis
mutant
anther culture
in vitro
cross-pollination
hybridization
indica rice
somatic cell
Oryza sativa
rice
Opis:: Anther and somatic tissue culture in combination with mutagenesis were carried out to evaluate the efficiency of different mutagenic treatments of various in vitro culture materials, and to obtain some promising variants for rice improvement. Results indicated that in japonica rice radiation treatment of dry seeds and young panicles influenced the percentage of green plantlets regeneration from anther culture. Both treatments increased significantly the percentage of regenerated green plantlets in comparison with the control. Irradiation with 30 Gy of rice callus increased also the percentage of regenerated green plantlets. For indica rice, the combination of the suitable dose of gamma rays irradiation on seeds and an improved medium, increased the percentage of callus induction. This approach made it possible to use anther culture in indica rice breeding. Somatic tissue cultures combined with radiation-induced mutagenesis led to the development of a number of promising mutants including some new cytoplasm-nucleus interacting male-sterile lines with almost 100% stigma exsertion. Their development would be of practical significance for increasing the genetic diversity for production of hybrid rice.
Źródło:: Journal of Applied Genetics; 1998, 39, 2; 151-162
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 3.

Tytuł:: Anther culture response in F1 hybrids of winter wheat [Triticum aestivum L.]
Autorzy:: Ponitka, A
Slusarkiewicz-Jarzina, A
Powiązania:: https://bibliotekanauki.pl/articles/2047258.pdf
Data publikacji:: 1996
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: haploid production
plant regeneration
F1 hybrid
green plant
cold treatment
albino plant
wheat
anther structure
hybrid
Triticum aestivum
winter wheat
somatic embryo
Opis:: The effect of cold pretreatment of spikes on somatic embryo induction and anther culture response of 25 F₁ winter wheat hybrids was investigated. The efficiency of androgenic embryos was the highest when spikes were incubated at 4°C for 6-9 days. A total of 2242 (73.0%) green and 885 (27.0%) albino plants were obtained from 9900 cultured anthers. Anther culture response in wheat was found to be markedly affected by the genotype of donor plants. The percentage of green plants varied from 0 to 115.7%. A great majority of anther-derived regenerants were haploids (82.35%), while the remaining plants were spontanoeus diploids (13.73%) and aneuploids (3.92%).
Źródło:: Journal of Applied Genetics; 1996, 37, 3; 253-260
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 4.

Tytuł:: Is the recently discovered EDA gene associated with anhidrotic ectodermal dysplasia?
Autorzy:: Kobielak, K
Kobielak, A
Trzeciak, W H
Powiązania:: https://bibliotekanauki.pl/articles/2046683.pdf
Data publikacji:: 1997
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: ectodermal-mesodermal interaction
X chromosome
submicroscopic deletion
hyperthermia
Christ-Siemens-Touraine syndrome
hybridization
ectodermal dysplasia
mutation
somatic cell
linkage analysis
embryonic life
transmembrane receptor
Opis:: The evidence from literature strongly suggests that Christ-Siemens-Touraine (CST) syndrome is associated with mutations of the newly discovered EDA gene. The gene is situated on the long arm of the X chromosome (Xq12.2-q13.1) and contains two exons separated by a 200 kbp intron. The 5’-untranslated region and most of the coding sequence are localized in exon 1, while three C-terminal amino acids are encoded by exon 2. The coding sequence was interrupted by translocations in three affected females: t(X;l), t(X;12), t(X;9), and submicroscopic deletions of the EDA gene were found in five males with CST syndrome, and point mutations were discovered in exon 1 in nine other patients. Northern blot analysis and in situ hybridization studies revealed that the EDA gene was expressed in the foetus, and postnatally in a specific type of skin cell and that the expression was limited to cells of ectodermal origin. A predicted protein product of the EDA gene contains 135 to 140 amino acids, organized in three distinct domains and may belong to class II transmembrane receptors.
Źródło:: Journal of Applied Genetics; 1997, 38, 3; 343-357
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 5.

Tytuł:: Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber [Cucumis sativus L.]
Autorzy:: Plader, W
Hoshi, Y.
Malepszy, S.
Powiązania:: https://bibliotekanauki.pl/articles/2044260.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: heterochromatin
4',6-diamidino-2-phenylindole
euchromatin segment
hybridization
chromosome identification
mitotic chromosome
prometaphase chromosome
somatic cell
cucumber
DNA
chromomycin A3
Cucumis sativus
Opis:: Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A₃ (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA⁺) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA⁺DAPI⁻) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA⁺DAPI⁻ band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA⁺ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA⁺ band at the short arm and a clear CMA⁺DAPI⁻ band at the pericentromeric region. Chromosome 6 had a CMA⁺ band at the distal region and a weak CMA⁺ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA⁺ band in the long arm and a CMA⁺ DAPI⁻ band in the pericentromeric region.
Źródło:: Journal of Applied Genetics; 1998, 39, 3; 249-258
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 6.

Tytuł:: Cytogenetic markers of Brassica napus L. chromosomes
Autorzy:: Hasterok, R
Maluszynska, J.
Powiązania:: https://bibliotekanauki.pl/articles/2043142.pdf
Data publikacji:: 2000
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: oil seed
in situ
silver staining
chromosome number
morphology
molecular cytogenetics
in vitro
karyotype
rDNA
morphometric analysis
plant breeding
oilseed rape
Brassica napus
somatic hybridization
transformation
cytogenetic marker
fluorescence
hybridization
genomic origin
Źródło:: Journal of Applied Genetics; 2000, 41, 1; 1-9
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki

Artykuł

Zmień widok

na półce

Skocz do pozycji: 7.

Tytuł:: Somaclonal selection of physiological mutants and several problems related to rice cell breeding
Autorzy:: Kinoshita, T
Mori, K.
Powiązania:: https://bibliotekanauki.pl/articles/2044460.pdf
Data publikacji:: 1998
Wydawca:: Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:: chromosome variation
japonica rice
Oryza
African rice
physiological mutant
gamma-radiation
anther culture
hybrid plant
protoplast isolation
callus induction
callus formation
aneuploidy
indica rice
herbicide tolerance
hybrid
Kitaake cultivar
gametoclonal variation
somatic hybrid
rice
Opis:: Tolerance to a new herbicide, pyributycarb, was evaluated both at the plant and cellular levels. Several highly or moderately tolerant strains chosen at the plant level, showed a parallel relation of to tolerance at the cellular level. However, on the whole, correlation between total tolerance indices and survival rates of calli was not significant in 18 out of the 80 studied strains. As a result of somaclonal selection for two herbicides, lines NB-200 and NM-100 were regenerated from the tolerant calli screened with benthiocarb at 200 ppm, and molinate at 100 ppm, respectively. In the R₂ generation, both the lines displaned a stable tolerance both at the plant and cellular levels. Thus the highly tolerant mutant lines were developed from a moderately tolerant line, N-61, via in vitro selection. To achieve a short-cut method in the interspecific genetic exchange, a series of techniques related to cell fusion were established in rice and related species. Two kinds of somatic hybrids between the cultivar Kitaake and tetraploid Oryza species, O. punctata and O. officinalis, were successfully produced. Among the somatic hybrid plants, a wide range of chromosomal variation was observed. Aneuploid plants with a chromosome number around 2n = 72 (hexaploid), which are expected from a symmetric fusion between diploid and tetraploid strains, were obtained showing mixoploidy within a plant. Most of the somatic hybrids were characterized by intermediate features of plant-type showing high sterility, shattering of spikelets and reduced plant height. As an exception, a diploid plant, which was identified by RFLP analysis using the rDNA gene probe, closely resembled Kitaake and produced viable seeds. A tetraploid hybrid plant was also promising for the introduction of economically important characters through the reduction of chromosome numbers by doubled haploids. Gametoclonal variation and gamma radiation was applied to Kitaake. The mutation frequency was prominently increased by gamma ray treatment, especially at high doses of 200 Gy or 300 Gy. In the M₃R₂ or M₄R₃ generations, most of the variants showed unfavourable characters. Most of the mutant characters were governed by single or double recessive genes. Several mutants such as short culm and early flowering time might be used for rice breeding.
Źródło:: Journal of Applied Genetics; 1998, 39, 1; 9-35
1234-1983
Pojawia się w:: Journal of Applied Genetics
Dostawca treści:: Biblioteka Nauki