Temat: DPPH· - Katalog OPAC zbiorów

Skocz do pozycji: 1.

Tytuł:: Determination of antiradical and antioxidant activity: basic principles and new insights
Autorzy:: Tirzitis, Gunars
Bartosz, Grzegorz
Powiązania:: https://bibliotekanauki.pl/articles/1040394.pdf
Data publikacji:: 2010
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: DPPH
ABTS
hydroxyl radical
antiradical
antioxidant
Opis:: Although the term 'antioxidant' is used very frequently, there are problems with the definition of antioxidants and estimation of antioxidant activity. The distinction between antioxidant and antiradical activities is not always obvious. This minireview discusses critically the principles, advantages and limitations of the most frequently used methods of estimation of antiradical and antioxidant activities.
Źródło:: Acta Biochimica Polonica; 2010, 57, 2; 139-142
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 2.

Tytuł:: Antioxidant capacity of crude extracts containing carotenoids from the berries of various cultivars of Sea buckthorn (Hippophae rhamnoides L.)
Autorzy:: Kruczek, Michał
Świderski, Adam
Mech-Nowak, Aleksandra
Król, Katarzyna
Powiązania:: https://bibliotekanauki.pl/articles/1039800.pdf
Data publikacji:: 2012
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: carotenoids
FRAP
antioxidant capacity
DPPH
sea buckthorn
Opis:: Comparative analysis of antioxidant capacity was performed using FRAP and DPPH methods on extracts containing carotenoids acquired from fruits of Sea buckthorn. The examination included nine varieties of Sea buckthorn growing in the comparative cultivation. Conducted analysis allowed to compare the antioxidant capacity with carotenoids content measured with spectrophotometric and HPLC methods. Three of the examined cultivars indicating high antioxidant activity in both, FRAP and DPPH methods, also revealed highest ('Aromatnaya') and high ('Botanicheskaya', 'Arumnyj') total carotenoids content in HPLC analysis.
Źródło:: Acta Biochimica Polonica; 2012, 59, 1; 135-137
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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Skocz do pozycji: 3.

Tytuł:: Influence of 2,5-dichloro-1,4-benzoquinone on jack bean urease activity. Inhibitory effect, total reducing capacity and DPPH radical scavenging activity
Autorzy:: Kot, Mirosława
Olech, Zofia
Powiązania:: https://bibliotekanauki.pl/articles/1039867.pdf
Data publikacji:: 2011
Wydawca:: Polskie Towarzystwo Biochemiczne
Tematy:: reducing capacity
inhibition
urease
Folin-Ciocalteu method
2,5-dichloro-1,4-naphthoquinone
DPPH scavenging activity
Opis:: Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inactivated. Application of Wilson-Kitz method proved that the urease-DCBQ interaction followed a simple bimolecular process and the presence of intermediate complex was undetectable. The determined second order rate constant of the inactivation was 0.053 (μM min)-1. Thiols such as l-cysteine, glutathione and dithiothreitol (DTT) protected urease from inhibition by DCBQ but DCBQ-modified urease did not regain its activity after DTT application. The thiol protective studies indicated an essential role of urease thiol(s) in the inhibition. The irreversibility of the inactivation showed that the process was a result of a direct modification of urease thiol(s) by DCBQ (DCBQ chlorine(s) substitution). The decomposition of DCBQ in aqueous solution at natural light exposure was monitored by visible spectrophotometry, determination of the total reducing capacity (Folin-Ciocalteu method) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging ability. The DCBQ conversion resulted in a decrease of the inhibition power and was well correlated with the increase of the total reducing capacity and DPPH scavenging ability. These findings were attributed to DCBQ transformation by photolysis and the hydrolysis effect was found to be negligible.
Źródło:: Acta Biochimica Polonica; 2011, 58, 4; 627-633
0001-527X
Pojawia się w:: Acta Biochimica Polonica
Dostawca treści:: Biblioteka Nauki

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