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Wyświetlanie 1-15 z 36
Tytuł:
Heavy metals in the cell nucleus - role in pathogenesis
Autorzy:
Sas-Nowosielska, Hanna
Pawlas, Natalia
Powiązania:
https://bibliotekanauki.pl/articles/1039119.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
heavy metal
nucleus
chromatin
DNA
RNA
telomere
Opis:
People are exposed to heavy metals both in an occupational and natural environment. The most pronounced effects of heavy metals result from their interaction with cellular genetic material packed in form of chromatin. Heavy metals influence chromatin, mimicking and substituting natural microelements in various processes taking place in the cell, or interacting chemically with nuclear components: nucleic acids, proteins and lipids. This paper is a review of current knowledge on the effects of heavy metals on chromatin, exerted at the level of various nuclear components.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 7-13
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Abscisic acid - an overlooked player in plant-microbe symbioses formation?
Autorzy:
Stec, Natalia
Banasiak, Joanna
Jasiński, Michał
Powiązania:
https://bibliotekanauki.pl/articles/1038840.pdf
Data publikacji:
2016
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
abscisic acid
symbioses
nodulation
arbuscular mycorrhiza
Opis:
Abscisic acid (ABA) is an ubiquitous plant hormone and one of the foremost signalling molecules, controlling plants' growth and development, as well as their response to environmental stresses. To date, the function of ABA has been extensively investigated as an abiotic stress molecule which regulates the plants' water status. However, in the context of symbiotic associations, ABA is less recognized. In contrast to well-described auxin/cytokinin and gibberellin/strigolactone involvement in symbioses, ABA has long been underestimated. Interestingly, ABA emerges as an important player in arbuscular mycorrhiza and legume-rhizobium symbiosis. The plant's use of stress hormones like ABA in regulation of those interactions directly links the efficiency of these processes to the environmental status of the plant, notably during drought stress. Here we provide an overview of ABA interplay in beneficial associations of plants with microorganisms and propose ABA as a potential factor determining whether the investment in establishing the interaction is higher than the profit coming from it.
Źródło:
Acta Biochimica Polonica; 2016, 63, 1; 53-58
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Synthesis of a quaternary polyprenyl ammonium salt
Autorzy:
Sizova, Olga
Utkina, Natalia
Kalinchuk, Nadezhda
Danilov, Leonid
Maltsev, Sergey
Powiązania:
https://bibliotekanauki.pl/articles/1040889.pdf
Data publikacji:
2007
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
polyprenol
quaternary ammonium salts
cationic lipids
Opis:
Reaction of primary C55-allylic alcohol moraprenol (WT3C7-9-OH, a polyprenol from mulberry leaves) with triethylamine in the presence of phosphorus oxychloride leads to a quaternary ammonium chloride with a good yield (72%) and high cis-stereoselectivity of the terminal isoprene unit. Cationic polyprenyl derivatives may be useful for transfection and immunological studies.
Źródło:
Acta Biochimica Polonica; 2007, 54, 4; 869-872
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of tunicamycin on the biogenesis of hepatitis C virus glycoproteins
Autorzy:
Reszka, Natalia
Krol, Ewelina
Patel, Arvind
Szewczyk, Boguslaw
Powiązania:
https://bibliotekanauki.pl/articles/1040320.pdf
Data publikacji:
2010
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
glycoproteins
glycosylation inhibition
hepatitis C virus
tunicamycin
Opis:
Hepatitis C virus (HCV) infects humans, with a prevalence around 3% of population, causing acute and chronic hepatitis and hepatocellular carcinoma. We studied the effect of inhibition of glycosylation on the assembly of the HCV particle. HCV possesses two envelope glycoproteins E1 and E2 that are highly modified by N-glycans. These glycan residues are crucial for viral entry and maturation of the progeny. Here, we examined the influence of inhibition of N-glycosylation on expression of E1 and E2. Since the propagation of HCV in cell culture is limited, we used a recombinant baculovirus producing viral-like particles in insect cells. Our data showed that blocking of N-glycan transfer to the nascent polypeptide chain with the antibiotic tunicamycin resulted in the loss of E1 and E2. We also found that a dose of tunicamycin that did not influence the cell viability significantly reduced the E2 level in infected cells. The results indicate that blocking of glycosylation at an early step efficiently reduces the assembly of HCV virions. Thus, we suggest that derivatives of tunicamycin that preferentially block glycosylation of viral proteins may become potential therapeutic agents against HCV.
Źródło:
Acta Biochimica Polonica; 2010, 57, 4; 541-546
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Size, shape and secondary structure of calponin.
Autorzy:
Czuryło, Edward
Eimer, Wolfgang
Kulikova, Natalia
Hellweg, Thomas
Powiązania:
https://bibliotekanauki.pl/articles/1044328.pdf
Data publikacji:
2000
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
dynamic light scattering
secondary structure prediction
calponin
circular dichroism
secondary structure estimation
Opis:
The overall size and shape of the chicken gizzard calponin (CaP) h1 molecule was investigated by dynamic light scattering (DLS) measurements. From the DLS experiments, a z-averaged translational diffusion coefficient is derived (5.75 ± 0.3) × 10-7cm2s-1, which corresponds to a hydrodynamic radius of 3.72 nm for calponin. The frictional ratio (1.8 for the unhydrated molecule and 1.5 for the hydrated one) suggests a pronounced anisotropic structure for the molecule. An ellipsoidal model in length 19.4 nm and with a diameter of 2.6 nm used for hydrodynamic calculations was found to reproduce the DLS experimental data. The evaluation of the secondary structure of CaP h1 from the CD spectra by two independent methods has revealed that it contains, on average, 23% helix, 19% β-strand, 18% β-turns and loops, and 40% of remainder structures. These values are in good agreement with those predicted from the amino-acid sequence. Predictions used for CaP h1 were applied to other isoforms of known sequences and revealed that all calponins share a common secondary structure. Moreover, the predicted structure of the calponin CH domain is identical to that found by X-ray studies of the spectrin, fimbrin and utrophin CH domains.
Źródło:
Acta Biochimica Polonica; 2000, 47, 3; 791-806
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Real-time PCR approach in dermatophyte detection and Trichophyton rubrum identification
Autorzy:
Kobylak, Natalia
Bykowska, Barbara
Nowicki, Roman
Brillowska-Dąbrowska, Anna
Powiązania:
https://bibliotekanauki.pl/articles/1039146.pdf
Data publikacji:
2015
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
fungal infections
dermatophytosis
DNA extraction
real-time PCR
Opis:
Dermatophytes are keratinophilic molds that infect human hair, nails and skin. Diagnosis of dermatophytosis is based on morphological, serological and biochemical features. However, identification is difficult and laborious due to similarities between microorganisms. Thus, there is considerable interest to develop mycological diagnostic procedures based on molecular biology methods. In this study, fast, two-step DNA extraction method and real-time PCR was used for detection of dermatophytes DNA using pan-dermatophyte primers and identification of Trichophyton rubrum from pure cultures. The applied method allowed correct detection of all dermatophytes and correct identification of Trichophyton rubrum in less than 2 hours.
Źródło:
Acta Biochimica Polonica; 2015, 62, 1; 119-122
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Possible role of aphidicolin-sensitive DNA polymerase(s) in N-methyl-N-nitro-N-nitrosoguanidine and bleomycin induced cellular repair synthesis and virus reactivation
Autorzy:
Mazurowa, Natalia
Anuszewska, Elżbieta
Koziorowska, Jadwiga
Powiązania:
https://bibliotekanauki.pl/articles/1045702.pdf
Data publikacji:
1991
Wydawca:
Polskie Towarzystwo Biochemiczne
Źródło:
Acta Biochimica Polonica; 1991, 38, 3; 295-302
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Evaluation of P1 substrate specificity of staphylococcal SplB protease
Autorzy:
Pustelny, Katarzyna
Stach, Natalia
Wladyka, Benedykt
Dubin, Adam
Dubin, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1039355.pdf
Data publikacji:
2014
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
serine protease
serine protease-like
SplB
Staphylococcus aureus
substrate specificity
Opis:
Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance. Virulence of S. aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role. Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role. Initial data demonstrated that Spl proteases exhibit restricted substrate specificity. This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position. Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.
Źródło:
Acta Biochimica Polonica; 2014, 61, 1; 149-152
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity
Autorzy:
Borodynko, Natasza
Hasiów-Jaroszewska, Beata
Rymelska, Natalia
Pospieszny, Henryk
Powiązania:
https://bibliotekanauki.pl/articles/1040498.pdf
Data publikacji:
2009
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
BSBV
genetic diversity
Polymyxa betae
Opis:
The complete nucleotide sequence of a Polish isolate of Beet soil-borne virus was determined for the first time. The genome organization was identical with those previously established for isolates from Germany and China. A comparison of the Polish isolate with others deposited in GenBank reveled high level of nucleotide identity, about 98-100%, throughout the genome analyzed. The ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. The non-synonymous mutations were particulary frequent in triple gene block.
Źródło:
Acta Biochimica Polonica; 2009, 56, 4; 729-731
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Prenyl sulfates as alkylating reagents for mercapto amino acids
Autorzy:
Maltsev, Sergey
Sizova, Olga
Utkina, Natalia
Shibaev, Vladimir
Chojnacki, Tadeusz
Jankowski, Wieslaw
Swiezewska, Ewa
Powiązania:
https://bibliotekanauki.pl/articles/1040690.pdf
Data publikacji:
2008
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cysteamine
geraniol
cysteine
S-prenylation
nerol
lipidated amino acids
Opis:
A new methodology for prenylation of thiol compounds has been developed. The approach is based on the use of prenyl sulfates as new reagents for S-prenylation of benzenethiol and cysteamine in aqueous systems. The C10-prenols geraniol and nerol that differ in the configuration (E or Z, correspondingly) of the α-isoprene unit were efficiently O-sulfated in the presence of a pyridine-SO3' complex. The obtained geranyl and neryl sulfates were tested as alkylating agents. These compounds were chosen to reveal the influence of the α-isoprene unit configuration on their alkylation (prenylation) ability. S-Geranyl cysteine was prepared to demonstrate the applicability of this method for prenylation of peptides containing mercapto amino acids.
Źródło:
Acta Biochimica Polonica; 2008, 55, 4; 807-813
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differences in editing of mitochondrial nad3 transcripts from CMS and fertile carrots.
Autorzy:
Rurek, Michał
Szklarczyk, Marek
Adamczyk, Natalia
Michalik, Barbara
Augustyniak, Halina
Powiązania:
https://bibliotekanauki.pl/articles/1044099.pdf
Data publikacji:
2001
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
cytoplasmic male sterility
Daucus carota
RNA editing
nad3 gene
Opis:
A high level of the nucleotide sequence conservation was found for mitochondrial nad3 gene of carrot. Three silent nucleotide substitutions differentiate nad3 open reading frames from cytoplasmic male sterile and male fertile carrots. All these differences are preserved on the RNA level. Partial and silent editing also distinguished both carrots. Three of the C to U conversions were specific to the fertile line. In the two examined carrot lines editing did not affect the mode of alteration of encoded amino acids.
Źródło:
Acta Biochimica Polonica; 2001, 48, 3; 711-717
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Characterization of conditions and determination of practical tips for mtDNA level estimation in various human cells
Autorzy:
Jędrak, Paulina
Sowa, Natalia
Barańska, Sylwia
Węgrzyn, Grzegorz
Powiązania:
https://bibliotekanauki.pl/articles/1038562.pdf
Data publikacji:
2017
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
mtDNA level
leukocytes
fibroblasts
qPCR methodology
Huntington disease
Opis:
Determination of mtDNA copy number in the cell is crucial to understand many cellular processes. Recently, the number of studies with the use of mitochondrial DNA (mtDNA) content as the determinant of mitochondrial abnormalities increased greatly and is still growing, therefore, optimization of technical conditions for this analysis is crucial. Despite using similar laboratory protocols, some results cannot be compared between research centers, thus causing discrepancies in the assessment of mtDNA content. The aim of this work was to test which conditions of biological sample collection and storage affect estimation of mtDNA level relative to the nuclear DNA (nDNA) in the blood samples and dermal fibroblasts. We found that the time and temperature of sample storage, as well as the type of the blood sample (whole blood or leukocytes) influence the estimate of mtDNA/nDNA ratio in the blood. In the case of dermal fibroblasts collected from healthy control and Huntington disease patients, our data indicate that the passage number of cells is essential to obtain reliable results.
Źródło:
Acta Biochimica Polonica; 2017, 64, 4; 699-704
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The favourable effect of catechin in electrochemotherapy in human pancreatic cancer cells
Autorzy:
Michel, Olga
Przystupski, Dawid
Saczko, Jolanta
Szewczyk, Anna
Niedzielska, Natalia
Rossowska, Joanna
Kulbacka, Julita
Powiązania:
https://bibliotekanauki.pl/articles/1038385.pdf
Data publikacji:
2018
Wydawca:
Polskie Towarzystwo Biochemiczne
Tematy:
electroporation
cisplatin
catechin
pancreatic cancer
in vitro
Opis:
Until recently, green tea polyphenols were considered strong antioxidants. However, the latest reports have revealed that bioflavonoids can play a multiple role in anticancer therapy, including the inhibition of cell proliferation and generation of the oxidative stress in a dose-dependent manner. The presented research was designed to examine the potential of the green tea (±)-catechin as a reinforcement of the electrochemotherapy (ECT) with cisplatin in pancreatic cancer in vitro. The study was performed on two cell lines of the pancreatic ductal adenocarcinoma (PDA) - parental EPP85-181P and multidrug-resistant EPP85-181RNOV. Prior to the ECT protocol the cells were preincubated with high or low concentration of catechin for 2 or 24 hours, respectively. We assessed the influence of preincubation on the cisplatin toxicity with and without electroporation (EP), the electrosensitivity of PDA cell lines and the uptake of the daunorubicin and propidium iodide. Additionally, we evaluated the antioxidative properties of catechin by the measurement of the ROS-related fluorescence and the immunoreactivity of the oxidative stress-related enzymes superoxide dismutase (SOD2) and glutathione S-transferase (GST). We found that co-treatment with catechin can firmly enhance the efficacy of electroporation with cisplatin in vitro. More favorable effect was obtained for 2-hour incubation, which indicates the involvement of the transcriptional-independent mechanisms of catechin action. The effect may be partially explained by the increased oxidative stress level, which was higher in multidrug-resistant cells. However, further studies on cisplatin-catechin interplay and the thorough examination of the catechin-cell membrane interaction need to be performed.
Źródło:
Acta Biochimica Polonica; 2018, 65, 2; 173-184
0001-527X
Pojawia się w:
Acta Biochimica Polonica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 36

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