- Tytuł:
- An improved method for RNA isolation from plants using commercial extraction kits
- Autorzy:
-
Kalinowska, Elżbieta
Chodorska, Maria
Paduch-Cichal, Elżbieta
Mroczkowska, Karolina - Powiązania:
- https://bibliotekanauki.pl/articles/1039717.pdf
- Data publikacji:
- 2012
- Wydawca:
- Polskie Towarzystwo Biochemiczne
- Tematy:
-
total RNA
secondary metabolites - Opis:
- Isolation of RNA from plants rich in secondary metabolites using commercial kits often results in contaminated preparations which are not suitable for downstream applications. Although many specific protocols appropriate for plants with a high content of phenolics, anthocyanins and polysaccharides have been developed, these are often expensive, time consuming and not applicable to different types of tissues. This study presents a simple and efficient modification of RNA extraction from different types of tissues using two commercial reagent kits. By simple improvement, we routinely obtained high-quality RNA of the following plants: the blackcurrant bush, black chokeberry bush, pear tree, apricot tree, apple tree, hardy kiwi, tangerine tree, highbush blueberry and cranberry plant.
- Źródło:
-
Acta Biochimica Polonica; 2012, 59, 3; 391-393
0001-527X - Pojawia się w:
- Acta Biochimica Polonica
- Dostawca treści:
- Biblioteka Nauki
Artykuł