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    Wyświetlanie 1-3 z 3
    Tytuł:
    Effect of Gluconacetobacter xylinus cultivation conditions on the selected properties of bacterial cellulose
    Autorzy:
    Fijałkowski, K.
    Żywicka, A.
    Drozd, R.
    Kordas, M.
    Rakoczy, R.
    Powiązania:
    https://bibliotekanauki.pl/articles/778981.pdf
    Data publikacji:
    2016
    Wydawca:
    Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
    Tematy:
    bacterial cellulose
    bacterial culture
    cultivation time
    culture conditions
    Gluconacetobacter xylinus
    Opis:
    The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.
    Źródło:
    Polish Journal of Chemical Technology; 2016, 18, 4; 117-123
    1509-8117
    1899-4741
    Pojawia się w:
    Polish Journal of Chemical Technology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Determination of volatile organochalcogens using liquid phase microextraction and gas chromatography with fluorine-induced chemiluminescence detection
    Autorzy:
    Hashemi, M.
    Montes, R. A.
    Chasteen, T. G.
    Powiązania:
    https://bibliotekanauki.pl/articles/363124.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Warmińsko-Mazurski w Olsztynie
    Tematy:
    bacterial culture
    fluorine-induced chemiluminescence
    gas chromatography
    microextraction
    volatile organochalcogens
    hodowla bakterii
    chemiluminescencja indukowana fluorem
    chromatografia gazowa
    mikroekstrakcja
    lotne organochalkogeny
    Opis:
    In this study, a simple and rapid liquid phase microextraction technique coupled with gas chromatography and fluorineinduced chemiluminescence detection has been developed for determination of volatile organochalcogens (dimethyl sulfide, dimethyl disulfide, dimethyl trisulfide and dimethyl diselenide and dimethyl telluride). The effects of extraction parameters such as extracting solvent and its volume, stirring time extraction time and salt effect were optimized. Also, analytical figures of merit such as response linearity, repeatability and reproducibility, and limits of detection have been evaluated. The proposed method was found to be a simple and rapid analytical procedure for determination of volatile organochalcogens in real aqueous samples with detection limits of 5-620ng·l-1. The relative recoveries of these analytes for spiked bacterial cultures, tap water and lake water ranged from 95 to 105% with the relative standard deviations of 0.3-11.0%.
    Źródło:
    Environmental Biotechnology; 2012, 8, 2; 55-62
    1734-4964
    Pojawia się w:
    Environmental Biotechnology
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    In situ-formed bacterial exopolysaccharide (EPS) as a potential carrier for anchorage-dependent cell cultures
    Autorzy:
    Komorowski, Piotr
    Kołodziejczyk, Agnieszka
    Makowski, Krzysztof
    Kotarba, Sylwia
    Walkowiak, Bogdan
    Powiązania:
    https://bibliotekanauki.pl/articles/1844871.pdf
    Data publikacji:
    2021
    Wydawca:
    Akademia Górniczo-Hutnicza im. Stanisława Staszica w Krakowie. Polskie Towarzystwo Biominerałów
    Tematy:
    bacterial exopolysaccharides
    dextran- -based “microcarriers”
    scanning electron microscopy
    atomic force microscopy
    roughness parameters
    three-dimensional cell culture
    Opis:
    The study involved the use of a bacterial strain isolated from environmental samples which produce the biopolymer in the form of pellets in the submerged culture. This material (bacterial exopolysaccharide) is produced by bacteria of the Komogateibacter xylinus which are prevalent in the environment. The aim of this study was to characterize bacterial exopolysaccharides and commercial dextran-based “microcarriers” in terms of their roughness and cell culture effects, including the morphology and viability of the human hybridoma vascular endothelial cell line EA.hy926. The pellets were characterized using scanning electron microscopy (SEM) and atomic for¬ce microscopy (AFM). The resulting structures were used for cell culture of adherent cells (anchorage¬-dependent cells). At the same time, the cultures with commercial, dextran-based “microcarriers” were carried out for comparative purposes. After com¬pletion of the cell culture (24 hours of culture), the cellulose and commercial “carriers” were analyzed using SEM and AFM. Finally, the obtained cell dens¬ities (fluorescence labelling) and their morphological characteristics (SEM) were compared. The obtained results strongly support the applicability of bacterial exopolysaccharide (EPS) in tissue engineering to build innovative 3D scaffolds for cell culture, the more so that it is technologically possible to produce EPS as spatially complex structure
    Źródło:
    Engineering of Biomaterials; 2021, 24, 159; 18-23
    1429-7248
    Pojawia się w:
    Engineering of Biomaterials
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-3 z 3

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