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Tytuł:
Study of the Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate (PHA) from Glucose
Autorzy:
Yasin, Akram R.
Al-Mayaly, Ithar K.
Powiązania:
https://bibliotekanauki.pl/articles/1955469.pdf
Data publikacji:
2021
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
polyhydroxyalkanoates
PHA
Bacillus cereus
biopolymer
glucose
Opis:
Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this study and demonstrated their efficiency in producing biopolymers.
Źródło:
Journal of Ecological Engineering; 2021, 22, 8; 41-53
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Jakość mikrobiologiczna rynkowych przypraw i ziół
Microbiological quality of market spices and herbs
Autorzy:
Berthold-Pluta, A.
Kurzyńska, D.
Powiązania:
https://bibliotekanauki.pl/articles/227907.pdf
Data publikacji:
2010
Wydawca:
Wyższa Szkoła Menedżerska w Warszawie
Tematy:
przyprawy
zioła
jakość mikrobiologiczna
Bacillus cereus
Opis:
Celem zaprezentowanych w artykule wyników pracy naukowo-badawczej było określenie ogólnej jakości mikrobiologicznej oraz występowania Bacillus cereus w 30 próbkach rynkowych przypraw i ziół, a także określenie wybranych cech biochemicznych i fizjologicznych wyizolowanych szczepów B. cereus. Ogólna liczba drobnoustrojów w przebadanych próbkach wahała się od 1,5x102 do 7,9x105 jtk/g, przy czym liczbę ponad 104 jtk/g stwierdzono w 40% próbek. Obecność Bacillus cereus w 1 g potwierdzono w 63,3% próbek, przy czym w połowie z nich najbardziej prawdopodobna liczba B. cereus nie przekraczała 101, a tylko w jednej próbce wynosiła ponad 103 w 1 g. Wszystkie 46 wyizolowane szczepy B. cereus rozkładały kazeinę. Zdolności do fermentacji laktozy i galaktozy nie wykazywało 91,3% i 82,6% szczepów, odpowiednio. Właściwości lipolityczne i amylolityczne wykazywało 63,0% i 82,6% szczepów, odpowiednio. Wszystkie szczepy wykazywały wzrost w warunkach 20, 30 i 37st. C/24 h, 78,3% szczepów w 43st. C/48 h, a 71,7% szczepów w 12st. C/3 dniach. Zdolności psychrotrofowe posiadało 23,9% szczepów (8st. C/10 dni), 6,5% szczepów (6st. C/10 dni) oraz 2,2% szczepów (4st. C/10 dni). Wszystkie badane próbki przypraw i ziół spełniały wytyczne ICMSF odnośnie OLD tj. <106 jtk/g, a poziom zanieczyszczenia B. cereus nie stwarzał niebezpieczeństwa zdrowotnego dla konsumentów (<104 jtk/g).
The aim of this work was to study the microbiological status of 30 retail samples of spices and herbs. The samples were examined among others for the presence of Bacillus cereus. The further aim of the study was to identify particular biochemical and physiological features of the isolated B. cereus strains. The total bacterial counts (TTC) for the samples ranged from 1.5x102 up to 7.9x105 cfu/g, however 40% of the samples had counts exceeding 104 cfu/g. The presence of B. cereus per 1g was detected in 63.3 % of the samples. However the most probable number of B. cereus population did not exceed 101 in half of the samples and the number exceeding 103 per 1 g was observed only in one sample. All 46 isolated B. cereus strains were capable of hydrolysing casein. It was also found that 91.3% and 82.6% of the strains did not exhibit the ability to ferment lactose and galactose, respectively. Furthermore, 63.0% and 82.6% of the strains respectively showed lipolytic and amylolytic activity. All the examined strains grew in the following conditions: 200, 300 and 37st. C/24 hr, 78.3% of the strains grew at 43 st. C/48 hr and 71.7% at 12 st. C/3 days. In this study 23.9% (8st. C/10 days) and 6.5% (6st. C/10 days) and 2.2% (4st. C/10 days) of the strains were identified as psychrotrophic. All the examined samples of spices and herbs conformed to the ICMSF specifications regarding TTC i. e. <106 cfu/g. It was concluded that the level of B. cereus contamination (<104 cfu/g) found in the samples did not pose any potential consumer health risk.
Źródło:
Postępy Techniki Przetwórstwa Spożywczego; 2010, 2; 42-46
0867-793X
2719-3691
Pojawia się w:
Postępy Techniki Przetwórstwa Spożywczego
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Polycarbonate biodegradation by newly isolated Bacillus strains
Autorzy:
Arefian, Mojgan
Tahmourespour, Arezoo
Zia, Mohammadali
Powiązania:
https://bibliotekanauki.pl/articles/205230.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biodegradation
Bacillus cereus
amylase
lipase
Polycarbonate
Bacillus megaterium
Opis:
As polycarbonate is frequently used in many products, its accumulation in landfills is absolutely harmful to the environment. The aims of this study were the screening and isolation of polycarbonate-degrading bacteria (PDB) and the assessment of their ability in the degradation of polycarbonate (PC) polymers. Nine-month buried- -PC films were used for PDB isolation and identification. The biodegradation ability of the isolates was determined by growth curve, clear zone formation, lipase and amylase production, AFM and FTIR. Bacillus cereus and Bacillus megaterium were identified and considered as PDB. The degradation ability of B. megaterium was significantly higher than that of B. cereus. Both were lipase and amylase positive. AFM and FTIR results showed the initiation of bacterial attachment. The PC biodegradation ability of isolates can be very efficient. Finding such efficient isolates (which was less studied before) will promise a decrease in plastic contamination in the future.
Źródło:
Archives of Environmental Protection; 2020, 46, 1; 14-20
2083-4772
2083-4810
Pojawia się w:
Archives of Environmental Protection
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Influence of the Culture Conditions on Lipolytic Activity of Bacillus cereus and Bacillus mycoides
Wpływ warunków środowiska na aktywność lipolityczną szczepów Bacillus cereus i Bacillus mycoides
Autorzy:
Nabradalik, M.
Grata, K.
Powiązania:
https://bibliotekanauki.pl/articles/388973.pdf
Data publikacji:
2011
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
Bacillus cereus
Bacillus mycoides
lipazy
tributyryna
Tween
lipases
tributyrin
Opis:
The aim of the research was the evaluation of lipolytic activity of B. cereus and B. mycoides strains, in reference to carbon source, pH and the temperature. In the research, two strains of Bacillus cereus and Bacillus mycoides each, isolated from the soil and water, were applied. The sources of carbon in culture media were fatty substrates: tributyrin, Tween 40, Tween 60, Tween 80 and glucose. The lipolytic activity was measured by means of titration at pH ranging from 5 to 8 and the temperature ranging from 30 oC to 60 oC. The results were presented in the units of lipolytic activity [U cm–3]. In the conducted research, the amount of liberated lipolytic activity depended on the type of fatty substrate in the medium, pH and the temperature. The strains under study showed the lowest activity at pH 5 and 6, and the highest at pH 7 and 8. In these conditions, most of the strains showed the lipolytic activity, even in case of the lack of fatty substrate in the medium. The highest amount of lipolytic activity was liberated at pH 8 in the medium with Tween 40, and the highest results (0.88 [U cm–3]) were noted for the soil strain B. cereus. When analysing the influence of the temperature on the lipolytic activity, it was stated that the highest amount of lipolytic activity was noted at 30 and 40 oC, and the lowest at 50 and 60 oC. The best results were obtained for most of the strains at 30 oC, in medium with Tween 40, and the most active was the soil strain of B. mycoides (0.88 [U cm–3]). The exception is B. cereus, as it liberated 1.38 [U cm–3], in the medium with glucose. Taking into account all analysed sources of carbon and parameters, it seems that the most active were B. mycoides strains.
Celem podjętych badań była ocena aktywności lipolitycznej szczepów B. cereus oraz B. mycoides w zależności od źródła węgla, pH oraz temperatury. Do badań wykorzystano 2 szczepy Bacillus cereus oraz 2 szczepy Bacillus mycoides wyizolowane z gleby i wody. ródłem węgla w pożywkach były substraty tłuszczowe: tributyryna, Tween 40, Tween 60, Tween 80 oraz glukoza. Aktywność lipolityczną oznaczono w zakresie pH od 5 do 8 oraz temperaturach od 30 do 60oC metodą miareczkową, a wyniki podano w jednostkach aktywności enzymatycznej [U cm-3]. W przeprowadzonym doświadczeniu aktywność lipolityczna uzależniona była od rodzaju substancji tłuszczowej zawartej w podłożu, pH oraz temperatury. I tak, badane szczepy B. cereus oraz B. mycoides wykazywały najmniejsza aktywność przy pH 5 oraz 6. Natomiast największą aktywność stwierdzono przy pH 7 i 8. W tych warunkach większość szczepów wykazywała aktywność lipolityczną nawet przy braku substratu tłuszczowego w podłożu. Największe wartości aktywności lipolitycznej uzyskano przy pH 8 na podłożu z dodatkiem Tween 40, a największe wartości (0,88 U cm-3) uzyskano dla glebowego szczepu B. cereus. Analizując wpływ temperatury na aktywność lipolityczną stwierdzono, iż najwyższą aktywność odnotowano w temperaturze 30 i 40 oC, a najmniejszą w 50 i 60 oC. Największe wartości, dla większości szczepów, uzyskano w temperaturze 30 oC na podłożu z dodatkiem Tween 40, gdzie najbardziej aktywnym okazał się glebowy B. mycoides (0,88 U cm-3). Wyjątek stanowi glebowy B. cereus, dla którego wartość aktywności lipolitycznej na podłożu z dodatkiem glukozy wynosiła aż 1,38 U cm-3. Uwzględniając wszystkie analizowane źródła węgla i parametry, najaktywniejszymi były szczepy B. mycoides.
Źródło:
Ecological Chemistry and Engineering. A; 2011, 18, 12; 1727-1736
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Skuteczność wybranych związków dezynfekcyjnych wobec przetrwalników Bacillus
Activity of selected compounds against spores of Bacillus
Autorzy:
Olesiak, P.
Stępniak, L.
Powiązania:
https://bibliotekanauki.pl/articles/297164.pdf
Data publikacji:
2012
Wydawca:
Politechnika Częstochowska. Wydawnictwo Politechniki Częstochowskiej
Tematy:
dezynfekcja
endospory
Bacillus subtilis
Bacillus mycoides
Bacillus cereus
disinfectants
endospores
Opis:
Badano wpływ trzech często stosowanych w antyseptyce i dezynfekcji związków: kwasu nadoctowego, nadtlenku wodoru w stężeniu 5 i 30% oraz aldehydu glutarowego na przetrwalniki bakterii. Kwas nadoctowy stanowił główny składnik preparatu komercyjnego pod nazwą Steridial P. Natomiast aldehyd glutarowy stosowano w postaci preparatu handlowego o nazwie Lysoformin 3000. Skuteczność dezynfektantów badano wobec przetrwalników bakterii z rodzaju Bacillus: B.subtilis, B.mycoides oraz B.cereus. Endospory, czyli przetrwalniki, są formami bakterii, które pozwalają im przeżyć skrajnie niekorzystne dla nich warunki, takie jak: suszę, wysoką temperaturę czy działanie środków dezynfekcyjnych. Zawdzięczają to odmiennej od komórek wegetatywnych budowie. Wykazano, że testowane związki są bardzo silnymi biocydami. Działanie sporobójcze wobec badanych szczepów obserwowano po trzydziestominutowej ekspozycji na każdy z testowanych związków.
The effect of three frequently used in antyseptic and disinfection of: peracetic acid, hydrogen peroxide at a concentration of 5 and 30%, and glutaraldehyde. Peracetic acid was the main ingredient Steridial P, in addition to acetic acid and hydrogen peroxide. Hydrogen peroxide in the form of 5% is much stronger than what we use hydrogen peroxide to antiseptic wounds in our homes. However, glutaraldehyde has performed under the trade name as Lysoformin 3000. Disinfecting measures are an especially important role in maintaining cleanliness in hospitals but also in our homes. Also are needed in the pharmaceutical and grocery industry. Thanks it did reduce the incidence of some diseases, and sometimes eliminate them. Disinfectants allow us to fight drug-resistant strains of bacteria, which occur in hospitals.The effectiveness of disinfectants affected by many factors. Their role in our lives is invaluable. Disinfectants were used against bacterial spores of the genus Bacillus: B.subtilis, B.mycoides and B.cereus. Bacillus-genus bacteria occur everywhere: in water, air and soil are transferred along with objects, and air movements on human hands. So it's easy to get infected. Endospores are forms of bacteria that allow them to survive extremely unfavorable to them, conditions such as drought, high temperatures or the action of disinfectants. Construction disputes differs significantly from the construction of cell vegetative. The most important part of sports is the cover phones, which represent up to 50% of the dry matter (d.m.) of the spore. It is the presence of these creations provides endosporom such "integrity". Guards are constructed primarily of proteins containing large amounts of cysteine. Endospores are forms very dehydrated, the water is only 15% of all cells. Compared with cell vegetative, spores have also about almost half proteins more and 75% carbohydrates less. They do not have beta-hydroxybutyrate, which is to 1/3 d.m. cells vegetative. In construction there are large quantities of endospores dipikolinic acid (DPA), associated mainly with calcium ions, but also other divalent elements. Complexes of Ca2+ - DPA may constitute up to 10% d.m. all disputes. DPA is the most frequently isolated from the mantle. This compound is not present in vegetative cells and spores is responsible for resistance to UV radiation from 5 to 50 times greater (depending on the strain) than vegetative forms. The lower sensitivity to ultraviolet radiation also correspond to protein family SASP (small amidesoluble proteins). They change the structure of DNA-stiffen and straighten - through the saturation of these biomolecules is on the outer side of the helix. These proteins are present only in disputes, and are destroyed during germination. Spore cell wall is much thicker, otherwise refracts light and is composed of many layers, so that is poorly permeable. It was shown that the tested compounds are very potent biocides. Sporicidial action against all tested strains was observed after a thirty-minute exposure to each of the tested.
Źródło:
Inżynieria i Ochrona Środowiska; 2012, 15, 1; 41-50
1505-3695
2391-7253
Pojawia się w:
Inżynieria i Ochrona Środowiska
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Isolation and Identification of Native Bacteria from Total Petroleum Hydrocarbon Polluted Soil in Wonocolo Public Oilfields, Indonesia
Autorzy:
Sari, Gina Lova
Trihadiningrum, Yulinah
Ni’matuzahroh, -
Powiązania:
https://bibliotekanauki.pl/articles/952448.pdf
Data publikacji:
2019
Wydawca:
Polskie Towarzystwo Inżynierii Ekologicznej
Tematy:
Bacillus sp.
Bacillus cereus
TPH
polluted soil
Wonocolo public oilfields
Opis:
The presented study concerns on isolation and identification of indigenous bacteria in total petroleum hydrocarbon (TPH) polluted soil.. The composite TPH polluted soil was collected from Wonocolo public oilfields, Indonesia.. Pour plate and plate count techniques were used to bacterial population analysis and enumeration, respectively. Two dominant bacterial colonies were isolated from 4.06x107 CFU/g population in polluted soil, then morphologically and biochemically were characterized using Microbact Identification Kits (MicrobactTM GNB12A and 12B). The identification of isolated bacteria was performed using Bergey’s Manual of Determinative Bacteriology. The results showed that the strains of bacteria are Bacillus sp. and B. cereus with probability of 72.00 and 77.00%, respectively. These strains potentially acted as biosurfactant producers and hydrocarbon degraders. Thus, biostimulation could be implemented to reduce the TPH levels in polluted soil at Wonocolo public oilfields.
Źródło:
Journal of Ecological Engineering; 2019, 20, 8; 60-64
2299-8993
Pojawia się w:
Journal of Ecological Engineering
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Wpływ Escherichia coli na wzrost Bacillus cereus
The effect of Escherichia coli on growth of Bacillus cereus
Autorzy:
Stec, E.
Powiązania:
https://bibliotekanauki.pl/articles/874920.pdf
Data publikacji:
1991
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
produkty spozywcze
antagonizm bakteryjny
drobnoustroje
Escherichia coli
Bacillus cereus
Źródło:
Roczniki Państwowego Zakładu Higieny; 1991, 42, 1
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Effect of different environmental factors on amylolytic activity of Bacillus cereus and Bacillus mycoides
Wpływ różnych czynników środowiskowych na aktywność amylolityczną Bacillus cereus i Bacillus mycoides
Autorzy:
Grata, K.
Nabrdalik, M.
Latała, A.
Powiązania:
https://bibliotekanauki.pl/articles/127324.pdf
Data publikacji:
2010
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
Bacillus cereus
Bacillus mycoides
amylolytic activity
pH
temperature
aktywność amylolityczna
temperatura
Opis:
The influence of different factors on amylolytic activity Bacillus cereus (2 strains) and Bacillus mycoides (2 strains), isolated from soil samples and water of Turawa Lake has been studied. Effect of physiological (temperature of reaction from 30 to 60 and pH of reaction mixture in the range of 4.0÷8.0) and nutritional parameters (the carbon sources and they concentration in medium) were examined spectophotometricaly for their effect on amylase production. The amylase activity was estimated on the basis of the reduction in blue colour intensity resulting from enzymatic hydrolysis of starch. The results obtained, that in view of all studied factors, the best pH level appear 7.0 or 8.0 and temperature 60ºC for all tested strains. Moreover amylase yield was the highest in media containing maltose or glucose as sole carbon source in all tested temperature.
Celem badań była ocena wpływu różnych czynników na aktywność amylolityczną Bacillus cereus (2 szczepy) i Bacillus mycoides (2 szczepy), wyizolowanych z gleby i jeziora Turawa. Badano wpływ pH mieszaniny reakcyjnej w zakresie od 5,0 do 8,0 i temperatury w zakresie od 30 do 60°C oraz źródła węgla i jego koncentracji w podłożu na poziom amylaz. Na podstawie stopnia zmniejszenia się zabarwienia z jodem oznaczono ilość rozłożonej skrobi metodą spektrofotometryczną. Uzyskane wyniki badań wykazały, iż spośród przebadanych czynników dla wszystkich szczepów najlepszą wartością pH była 7,0 lub 8,0, a temperatura 60ºC. Ponadto najwyższy poziom amylaz uzyskano, hodując badane szczepy na podłożu z maltozą lub glukozą w zakresie badanych temperatur.
Źródło:
Proceedings of ECOpole; 2010, 4, 1; 49-53
1898-617X
2084-4557
Pojawia się w:
Proceedings of ECOpole
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Growth characteristics and antagonistic potential of Bacillus cereus and Bacillus zhangzhouensis against pathogenic bacteria Aeromonas hydrophila in vitro
Autorzy:
Sofandi, Adrianti Nur Fitria
Mulyani, Yuniar
Rochima, Emma
Rosidah, Rosidah
Powiązania:
https://bibliotekanauki.pl/articles/1193481.pdf
Data publikacji:
2021
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
Aeromonas hydrophila
Bacillus cereus
Bacillus zhangzhouensis
antimicrobial activity
pathogenic bacteria
Opis:
This study aims to examine the growth characteristics of B. cereus and B. zhangzhouensis and to see their potential in preventing the growth of Aeromonas hydrophila in vitro. This research was conducted in June 2020 - May 2021 at the Laboratory of Microbiology and Biotechnology, Faculty of Fisheries and Marine Sciences, Padjadjaran University. The method used is the exploratory method and the data obtained were analyzed descriptively. The growth curve was performed using the turbidimetric method. The inhibition test used the disc diffusion method with 5 treatments, chloramphenicol antibiotics (4000 ppm), distilled water, bacterial culture, 100% supernatant and 50% supernatant. The curve results showed that the growth of B. cereus and B. zhangzhouensis was slow. The stationary phase of B. cereus occurred at 40-72 hours, while in B. zhangzhouensis the stationary phase occurred at 48-72 hours. The results of the inhibition test showed that the antimicrobial activity of B. cereus and B. zhangzhouensis was weak with a value range of 0.71 - 1.6mm, besides that the results of the inhibition zone produced by these two bacteria have different inhibitory abilities in each treatment.
Źródło:
World Scientific News; 2021, 158; 187-200
2392-2192
Pojawia się w:
World Scientific News
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Physical factors affecting the production of amylase from Bacillus species isolated from natural environment
Wpływ czynników fizycznych na aktywność amylolityczną Bacillus sp. wyizolowanych ze środowiska naturalnego
Autorzy:
Grata, K.
Nabrdalik, M.
Powiązania:
https://bibliotekanauki.pl/articles/389748.pdf
Data publikacji:
2014
Wydawca:
Towarzystwo Chemii i Inżynierii Ekologicznej
Tematy:
Bacillus cereus
Bacillus mycoides
amylase
physical factor
amylazy
czynniki fizyczne
Opis:
The influence of different factors on amylolytic activity Bacillus cereus (2 strains) and Bacillus mycoides (2 strains), isolated from soil samples and water of Turawa Lake has been studied. Effect of physiological (pH of reaction mixture in the range of 4.0–8.0, temperature of reaction from 30 to 60 oC) and nutritional parameters (the carbon sources and they concentration in medium) were examined spectophotometricaly for their effect on amylase production. The results obtained showed, that in view of all studied factors the least favorable value of pH was 5.0, while in most cases the most favourable for the process of amylase production were following values 7.0 and 8.0. Moreover, it has been stated that the lowest amount of amylase was noted at 30 oC, and the highest at 60 oC. In case of amylolytic activity, in view of all temperatures the most effective strain was B. mycoides A134 whereas the least B. mycoides G3. The best results of amylase production have been achieved for all remaining strains in two cases – on maltose and glucose medium at all tested temperatures. Additionally, soil occurring strains preferred lower concentration of potato starch (ie 1 %) when compared with water strains which favoured higher concentration (5 %).
Celem badań była ocena wpływu różnych czynników na aktywność amylolityczną Bacillus cereus (2 szczepy) i Bacillus mycoides (2 szczepy) wyizolowanych z gleby i Jeziora Turawskiego. Metodą spektrofotometryczną badano wpływ pH mieszaniny reakcyjnej w zakresie od 5.0 do 8.0 i temperatury w zakresie od 30 oC do 60 oC oraz źródła węgla i jego koncentracji w podłożu na poziom produkowanych amylaz. Stwierdzono, iż najgorszą wartością pH do syntezy amylaz było 5.0, natomiast najlepszą, w większości przypadków pH 7.0. Uwzględniając zakres badanych temperatur, najwyższą aktywność uzyskano w temp. 60 oC, natomiast najniższą w temp. 30 oC dla wszystkich testowanych szczepów. Najbardziej aktywnym szczepem był B. mycoides A 134, natomiast najmniej B. mycoides G3.
Źródło:
Ecological Chemistry and Engineering. A; 2014, 21, 1; 51-60
1898-6188
2084-4530
Pojawia się w:
Ecological Chemistry and Engineering. A
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Value addition to Pongamia biodiesel industry through bioethanol production from pressed oil cake using Bacillus cereus
Autorzy:
Venkatesh, K.H.
Rao, V.C.
Powiązania:
https://bibliotekanauki.pl/articles/2096993.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
Pongamia pinnata
oil cake hydrolysate
bioethanol
Bacillus cereus
value addition
Opis:
Pongamia pinnata, a tree bearing oil seeds, has been one of the prime focus among nonedible oil sources for biodiesel production in most parts of India. The oil cake obtained after the extraction of oil serves as an adequate source of cellulose, hemicellulose, proteins, lipids, and starch. The scarce literature data available suggest very low conversion rate of complex sugars to fermentable sugars and further to bioethanol. In the present work, a sulfuric acid hydrolysate obtained from Pongamia oil cake (POC) under microwave irradiation was anaerobically fermented using Bacillus cereus ATCC 14579. Under anaerobic and acidic conditions, the process parameters affecting the bioethanol production efficiency (BPE) were screened and optimized using statistical experimental designs. Of the seven parameters screened (glucose concentration, yeast extract as nitrogen source, agitation speed, pH, inoculum size, fermentation time, and temperature), only 3 were found to be significant and to increase BPE. The significant parameters were optimized using the Box-Behnken experimental design to obtain an optimal condition of 9 g/l reducing sugars, pH 4.6 maintained with acetate buffer, and fermentation period of 51 h that yielded 92% BPE with 4.2 g/l ethanol. Moreover, it was observed that the use of a buffer system to maintain broth pH, especially acetate buffer, significantly improved BPE. Low concentration of fermentation inhibitors is the key factor for improved BPE. The research outcome suggests that the production of dual fuel from Pongamia pinnata, viz. biodiesel and bioethanol, could not only improve the process economics but also reduce waste generation.
Źródło:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology; 2020, 101, 2; 147-158
0860-7796
Pojawia się w:
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Proteomic profile to explain the mechanism of the Bacillus cereus-phosphate mineral interaction
Autorzy:
El-Ghammaz, Mai Roshdy
Abdel-Khalek, Nagui Ali
Hassan, Mohamed Kamel
Powiązania:
https://bibliotekanauki.pl/articles/1447141.pdf
Data publikacji:
2021
Wydawca:
Politechnika Wrocławska. Oficyna Wydawnicza Politechniki Wrocławskiej
Tematy:
bio-flotation
phosphate
bacillus cereus
proteomic profile
S-layer proteins
Opis:
Bacillus cereus bacteria and their by-products were used as surface-active agents for surface hydrophobicity of the apatite in the flotation process leading to phosphate ores’ enrichment. Recently, proteomics is used to investigate the biochemical processes through discovering new proteins or investigating protein-protein interactions. In this work, we investigated the physicochemical behavior of pure apatite and quartz minerals in the presence of Bacillus cereus using zeta-potential, FTIR, and hydrophobicity measurements. Our results indicated that isoelectric point (IEP) occurred at pH 4.7 for apatite and 2.1 for quartz mineral. Bacillus cereus treatment decreased IEP of apatite to 1.8; while there was no significant change in IEP value of quartz. We used comprehensive proteomic profile analysis of Bacillus cereus in the presence of apatite mineral to identify the biological mechanism and molecules involved in such enrichment capacity. Our data identified the up-regulated Surface Layer (S-Layer) protein in this bacterial strain to be associated with the best mineral yield.
Źródło:
Physicochemical Problems of Mineral Processing; 2021, 57, 3; 136-150
1643-1049
2084-4735
Pojawia się w:
Physicochemical Problems of Mineral Processing
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Enzymatic bioconversion of feather waste with keratinases of Bacillus cereus PCM 2849
Autorzy:
Ciurko, Dominika P.
Łaba, Wojciech
Piegza, Michał
Juszczyk, Piotr
Choińska-Pulit, Anna
Sobolczyk-Bednarek, Justyna
Powiązania:
https://bibliotekanauki.pl/articles/778229.pdf
Data publikacji:
2019
Wydawca:
Zachodniopomorski Uniwersytet Technologiczny w Szczecinie. Wydawnictwo Uczelniane ZUT w Szczecinie
Tematy:
feather waste
Bacillus cereus
Box-Behnken design
hydrolysis
thermo-chemical pretreatment
Opis:
Enzymatic preparation from culture of keratinolytic Bacillus cereus PCM 2849 was applied for hydrolysis of whole chicken feathers, after sulphitolytic pretreatment. This process was optimized using a three-factor Box-Behnken design, where the effect of substrate concentration, sulphite concentration during pretreatment and reaction temperature was evaluated on the release of amino acids. Obtained results revealed the highest impact of reaction temperature, followed by substrate content and sulphite during pretreatment. Optimal process conditions were established, i.e. temperature 44.4°C, feathers 4.7% and treatment with 25.3 mM sulphite. Amino acid composition of the obtained hydrolysate was analyzed. Glutamic acid (9.21 g·kg−1) and proline were dominant, however significant amount of branched-chain amino acids was also observed. The FTIR analysis of residual substrate revealed the cleavage of disulphide bonds in keratin through the presence of thioester residues. The absence of reduced cysteine residues was confirmed, along with minor changes in proportions of keratin substructures.
Źródło:
Polish Journal of Chemical Technology; 2019, 21, 3; 53-59
1509-8117
1899-4741
Pojawia się w:
Polish Journal of Chemical Technology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Intensywność wytwarzania hemolizyn i lecytynazy przez szczepy Bacillus cereus wyizolowane z żywności
The intensity of production of hemolysin and lecithinase by Bacillus cereus strains isolated from food
Autorzy:
Stec, E.
Powiązania:
https://bibliotekanauki.pl/articles/874794.pdf
Data publikacji:
1990
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
aktywnosc hemolityczna
Bacillus cereus
zatrucia pokarmowe
hemolizyny
lecytyna
wyniki badan
Źródło:
Roczniki Państwowego Zakładu Higieny; 1990, 41, 3-4
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Differentiation of Bacillus anthracis and other Bacillus cereus group bacterial strains using multilocus sequence typing method
Autorzy:
Graniak, Grzegorz
Olender, Alina
Naylor, Katarzyna
Powiązania:
https://bibliotekanauki.pl/articles/1024269.pdf
Data publikacji:
2020-12-30
Wydawca:
Uniwersytet Łódzki. Wydawnictwo Uniwersytetu Łódzkiego
Tematy:
Bacillus cereus group
multilocus sequence typing
sequencing
housekeeping genes
phylogenetic differentiation
BioNumerics
Opis:
The study describes the preparation of the phylogenetic differentiation of Bacillus cereus strains. The Bacillus cereus group of bacteria is very important for human and animal health. The multilocus sequence typing scheme has been used to present this group of bacteria’s phylogenetic relationship and structure. The MLST system was established using 60 isolates of B. anthracis, B. cereus sensu stricto, B. thuringiensis, and transitional environment strains of Bacillus spp. As a negative control, five strains of B. subtilis and B. megaterium were used. Primers for amplification and sequencing were designed to target highly conserved internal fragment of seven housekeeping genes: glpF, gmk, ilvD, pta, pur, pycA, and tpi. A total of 22 different sequence types (STs) were distinguished. Analysis of the sequence data showed that all of the Bacillus cereus strains are very closely related. The MLST scheme exhibited a high level of resolution that can be used as an excellent tool for studying the phylogenetic relationship, epidemiology, and population structure of the Bacillus cereus group strains. The MLST method additionally allows us to define the phylogenetic relationship between very closely related strains based on a combination of the sequences of all seven alleles fragments and each of them separately. Thus, this genetic investigation tool is very useful in epidemiological investigation of potential military/ bioterrorist use of B. anthracis.
Źródło:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica; 2020, 16; 12-21
1730-2366
2083-8484
Pojawia się w:
Acta Universitatis Lodziensis. Folia Biologica et Oecologica
Dostawca treści:
Biblioteka Nauki
Artykuł
Wyświetlanie 1-15 z 27

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