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Wyświetlanie 1-15 z 53
Tytuł:
SEM and stereoscope microscope observations on the seeds of the Polish species of the genus Sorbus L. [Rosaceae]
Autorzy:
Maciejewska-Rutkowska, I
Bednorz, L.
Powiązania:
https://bibliotekanauki.pl/articles/57855.pdf
Data publikacji:
2004
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Rosaceae
Sorbus
botany
morphology
stereoscope microscope
scanning microscope
seed
Opis:
This paper presents the results of the study on seed morphology of five Polish species of the genus Sorbus L. (S. aria (L.) Crantz, S. aucuparia L. Emend. Hedl., S. chamaemespilus (L.) Crantz, S. intermedia (Ehrh.) Pers. and S. torminalis (L.) Crantz). Observations and measurements were carried out with stereoscope and scanning microscopes. Four types of seed microsculpture were distinguished on the ground of SEM observations. The key to determine Polish species, based on morphological characters of seeds is given too.
Źródło:
Acta Societatis Botanicorum Poloniae; 2004, 73, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
A method of magnetic field measurement in a scanning electron microscope using a microcantilever magnetometer
Autorzy:
Orłowska, Karolina
Mognaschi, Maria E.
Kwoka, Krzysztof
Piasecki, Tomasz
Kunicki, Piotr
Sierakowski, Andrzej
Majstrzyk, Wojciech
Podgórni, Arkadiusz
Pruchnik, Bartosz
di Barba, Paolo
Gotszalk, Teodor
Powiązania:
https://bibliotekanauki.pl/articles/220725.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
scanning electron microscope
magnetometry
microcantilever
Opis:
Scanning electron microscopy (SEM) is a perfect technique for micro-/nano-object imaging [1] and movement measurement [2, 3] both in high and environmental vacuum conditions and at various temperatures ranging from elevated to low temperatures. In our view, the magnetic field expanding from the pole-piece makes it possible to characterize the behaviour of electromagnetic micro- and nano-electromechanical systems (MEMS/NEMS) in which the deflection of the movable part is controlled by the electromagnetic force. What must be determined, however, is the magnetic field expanding from the e-beam column, which is a function of many factors, like working distance (WD), magnification and position of the device in relation to the e-beam column. There are only a few experimental methods for determination of the magnetic field in a scanning electron microscope. In this paper we present a method of the magnetic field determination under the scanning electron column by application of a silicon cantilever magnetometer. The micro-cantilever magnetometer is a silicon micro-fabricated MEMS electromagnetic device integrating a current loop of lithographically defined dimensions. Its stiffness can be calibrated with a precision of 5% by the method described by Majstrzyk et al. [4]. The deflection of the magnetometer cantilever is measured with a scanning electron microscope and thus, through knowing the bias current, it is possible to determine the magnetic field generated by the e-beam column in a defined position and at a defined magnification.
Źródło:
Metrology and Measurement Systems; 2020, 27, 1; 141-149
0860-8229
Pojawia się w:
Metrology and Measurement Systems
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Error analysis of current formated by precision voltage-current converter loaded by inductance
Autorzy:
Shkliarskyi, V.
Vasylyuk, V.
Balanyuk, Y.
Powiązania:
https://bibliotekanauki.pl/articles/114381.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
scanning microscope
cathode ray tube
microobject
luminous raster
current control errors
Opis:
In scanning microscope the cathode ray tube of high resolution is used. The screen of this tube is formed by raster consisting of 4096×4096 luminous elements, composing the image. This raster allows to form images of investigated microobject on the screen in a wide range without loss of resolution. The accuracy of imaging on the screen depends on the current setting errors in inductive coils of deflection system. The ways of reducing the static error of the current set in a voltage-current converter are proposed.
Źródło:
Measurement Automation Monitoring; 2015, 61, 8; 403-405
2450-2855
Pojawia się w:
Measurement Automation Monitoring
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Pollen morphology of Rosa gallica L. [Rosaceae] from Southern Poland
Autorzy:
Wronska-Pilarek, D
Boratynska, K.
Powiązania:
https://bibliotekanauki.pl/articles/58479.pdf
Data publikacji:
2005
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
French rose
Rosaceae
multivariate analysis
rose
Rosa gallica
pollen morphology
light microscope
scanning microscope
Opis:
The morphology of pollen grains of Rosa gallica was studied on the basis of material from 15 natural localities in southern Poland. It was ascertained that the diagnostic features of pollen grains of R. gallica were: the presence of deep, often Y-shaped striae in a linear arrangement; numerous perforations of various, rather big diameters on the bottom of striae; the occurrence of operculum on the membrane of ectocolpus; the presence of costae ectocolpi and fastigium in the area of endopori. The most significant differences among the particular samples studied concern the shape of grains. The differences between pollen grains of 15 individuals of Rosa gallica from southern Poland are generally slight. The most discriminating measured character is length of equatorial axis of pollen grain and length of ectocolpi and thickness of exine along polar axis. The remaining differentiate the individuals with smaller significance. The differences between Silesian samples and all the other examined ones may result from origin of different Pleistocene refugia.
Źródło:
Acta Societatis Botanicorum Poloniae; 2005, 74, 4
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The suitability of scanning electron microscopy in the evaluation of bone structure surfaces and selection of alloplastic materials for facial skeletal reconstruction
Autorzy:
Job, Katarzyna
Składzień, Jacek
Powiązania:
https://bibliotekanauki.pl/articles/1397288.pdf
Data publikacji:
2021
Wydawca:
Index Copernicus International
Tematy:
hydroxyapatite
scanning electron microscope
secondary electron detection
Opis:
Introduction: Functional and aesthetic problems can arise even from small losses created in the facial skeleton. Injuries and oncological surgeries are the most frequent causes of these losses within the facial skeleton. Advances in surgical interventions have allowed for ever-increasing degrees of resections, increasing oncological radicality as well as treatment effectiveness, providing the patient with the chance for a longer life. However, this subsequently requires the use of even more advanced reconstruction techniques in order to restore quality of life and comfort to the patient, as well as enable their return to professional and social activities. The necessity of reconstructive surgery applies not only to patients with cancer, but also to patients with impaired or failing sensory and organ function as a result of inflammatory conditions, injuries, or non-oncological surgeries. There are many available reconstruction procedures, which depend on the location of the loss, the type of tissue lost, the degree of loss and patient-dependent factors. Materials used in reconstruction surgeries may include the patients’ tissues when available, and artificial reconstruction materials otherwise. Material and methods: The analysis involved fragments of bone tissue removed during surgery. Due to the nature of the medical procedure and the inability to replant the tissue, it was regarded as medical waste. The preparations used were observed under an optical microscope and an electron scanning microscope, and a chemical analysis was performed. The chemical composition of samples was analysed using a low vacuum detector (LVD) at an accelerating voltage of 15 kV and 10 kV and at a spot size of 4 and 3.5. The observations were performed in a secondary electron (SE) detection system. Results: Observation of parameters under an optical microscope and of images obtained using an electron scanning microscope showed the presence of typical, compact bone tissue with varied surface shapes in each case (various degrees of unevenness and porosity). Chemical composition analysis confirmed the presence of compounds from the CaO-P2O5-H20 system. The Ca/P (calcium/phosphorus) ratio obtained from the chemical analysis varied from 1.33 to 2.1, and indicated a varied morphology of calcium phosphates forming the bone structures of the facial skeleton. Conclusions: 1. Calcium phosphates are characterised by excellent biocompatibility because of their chemical affinity to bone, and are ideal for the reconstruction of bone losses within the facial skeleton. 2. Biodegradable polymers have the highest functional potential among several groups of biomaterials used in tissue engineering because of their ability to be tailored individually, in addition to their high biocompatibility.
Źródło:
Polish Journal of Otolaryngology; 2021, 75, 4; 14-19
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Auditory ossicles in Scanning Electron Microscopy
Autorzy:
Wiatr, Agnieszka
Składzień, Jacek
Wiatr, Maciej
Powiązania:
https://bibliotekanauki.pl/articles/1397399.pdf
Data publikacji:
2020-05-12
Wydawca:
Index Copernicus International
Tematy:
middle ear surgery
ossicular chain
Scanning Electron Microscope
Opis:
Introduction: Knowledge about the physiology of a healthy middle ear is essential for understanding the activity and mechanics of the ear as well as the basics of ossiculoplasty. Trauma of the epithelial lining of the tympanic cavity as well as the ossicular chain may be the result of chronic inflammation and surgery. Depending on the observed changes of the middle ear lining, there are several types of distinguished chronic inflammatory changes: simple, with cholesteatoma, with the formation of inflammatory granulation tissue, in course of specific diseases. Purpose: The aim of the article is presentation of the microstructure and vasculature of the ossicular chain in the Scanning Electron Microscope. Particular attention is drawn to the anatomical aspects of the structure and connections of auditory ossicles as vital elements for reconstruction of the conduction system of the middle ear. Material and method: The analysis covered auditory ossicles standardly removed in accordance with the methodology of the investigated surgical procedures. The preparations were evaluated in a scanning electron microscope. Results: The exposure of bone surface promotes deep erosion. The advanced process of destruction of bone surface in the case of chronic otitis media correlates with a significant degree of damage to both the lining covering the auditory ossicles and that surrounding articular surfaces. Conclusions: (1) The ossicles in the image of the Scanning Electron Microscope are covered with lining. It passes from the surface of the ossicles to the vascular bundles, forming vascular sheaths; (2) Damage to lining continuity on the surface of the auditory ossicles promotes the rapid destruction of bone tissue in the inflammatory process; (3) The dimensions of the individual ossicles are respectively: malleus – 8.36 +/- 0.01, incus – 8.14 +/- 0.0, stapes – 3.23 +/- 0.01 mm. Behavior of the anatomical length of ossicular chain during tympanoplasty appears to be essential to maintaining adequate vibration amplitude of the conductive system of the middle ear.
Źródło:
Polish Journal of Otolaryngology; 2020, 74, 4; 1-7
0030-6657
2300-8423
Pojawia się w:
Polish Journal of Otolaryngology
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Scanning television optical microscope for diagnostics of microobjects in medicine
Skanujący telewizyjny optyczny mikroskop dla diagnostyki mikroobiektów w medycynie
Autorzy:
Prudyus, I.
Shkliarskyi, V.
Matiieshyn, Y.
Pedan, A.
Balanyuk, Y.
Vasylyuk, V.
Powiązania:
https://bibliotekanauki.pl/articles/328479.pdf
Data publikacji:
2013
Wydawca:
Polska Akademia Nauk. Polskie Towarzystwo Diagnostyki Technicznej PAN
Tematy:
scanning microscope
electron beam tube
microobject
diagnostics
mikroskop skanujący
lampa kineskopowa
makroobiekt
diagnoza
Opis:
Scanning television optical microscope (SТОМ) developed by authors will enable to diagnose microobjects which size exceeds 0,1 microns. Diagnostics can be carried out by supervision over researched microobjects during influence on them of various reagents and catalysts, and also definition of parameters of microobjects: the sizes, speed of movement, acceleration, growth rate, change of density, etc. Ways of the scanning television microscope construction which can be used for the biological microobjects research are analyzed. The Microscope capabilities are expanded due to a raster formation in television and little-frame modes. Ways of the raster size change are offered at preservation of the microobject image resolution.
Skanujący telewizyjny optyczny mikroskop (STOM) skonstruowany przez autorów pozwoli na diagnozowanie mikroobiektów których rozmiar nie przekracza 0,1 mikrona. Diagnoza może być przeprowadzona poprzez obserwowanie badanych mikroobiektów podczas oddziaływania różnych reagentów i katalizatorów, oraz w celu określenia parametrów fizycznych mikroobiektów: wielkości, prędkości, przyspieszenia ruchu, tempa wzrostu, zmian gęstości itp. Sposób skonstruowania skanującego telewizyjnego optycznego mikroskopu powala na badania i analizy mikroobiektów biologicznych. Możliwości mikroskopu zostały rozszerzone w stosunku do skanowania rastrowego i trybów mało-kadrowych. Zaproponowane zostały sposoby rastrowej zmiany rozmiaru ekranu przy zachowaniu rozdzielczości zdjęcia mikroobiektu.
Źródło:
Diagnostyka; 2013, 14, 3; 41-44
1641-6414
2449-5220
Pojawia się w:
Diagnostyka
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Determination of acceleration of different dynamic microobjects by the television scanning optical microscope
Autorzy:
Prudyus, I.
Matiieshyn, Y.
Powiązania:
https://bibliotekanauki.pl/articles/114123.pdf
Data publikacji:
2015
Wydawca:
Stowarzyszenie Inżynierów i Techników Mechaników Polskich
Tematy:
television scanning optical microscope
microscope field of view
acceleration of movement
Opis:
The acceleration of the dynamic microobjects (MO) movement, the motion of which is accelerated uniformly or non-uniformly is an important task for television measuring systems. Most existing measuring systems usually have a small range of acceleration with a short period of growth. Such period of growth of the acceleration is characteristic for many dynamic MO. Better instruments to determine the non-uniform velocity of the various objects are based on various laser devices. These instruments are contactless and high-speed enough. In the paper the basics of the television scanning optical microscope (TSOM) are presented. In the Chapter 2 the schematic diagram of the TSOM and its main functionalities are pointed out. In the Chapter 3 an adaptation of the TSOM for movement acceleration of microobjects is discussed. Modified structural and functional diagram of the instrument is presented.
Źródło:
Measurement Automation Monitoring; 2015, 61, 8; 410-412
2450-2855
Pojawia się w:
Measurement Automation Monitoring
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Hardware and software correction method of piezo scanner nonlinearity in scanning microscope
Sprzętowo-programowa metoda korekcji nieliniowości piezoskanera w mikroskopie skaningowym
Autorzy:
Pawłowski, S.
Majcher, A.
Mrozek, M.
Powiązania:
https://bibliotekanauki.pl/articles/256178.pdf
Data publikacji:
2013
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Technologii Eksploatacji - Państwowy Instytut Badawczy
Tematy:
scanning microscope
piezo scanner
piezo scanner nonlinearity correction
mikroskop skaningowy
piezoskaner
korekcja nieliniowości piezoskanera
Opis:
An inherent feature of the piezo scanner in scanning probe microscopy is their time-varying nonlinear. This results in a distortion of images and a difficulty of the measurements that require adequate control of the current position of the cantilever with respect to the sample surface. This paper presents methods of correcting a nonlinear piezo scanner. The advantages and disadvantages of each solution are pointed out, and the developed hardware and software correction methods are described. The correction is performed by a digital system made up of high-speed analogue-to-digital converters and a FPGA programmable system in which the correction algorithms are implemented. These algorithms determine, on the basis of actual measurement data, a set of correction factors for different areas and scanning speed. This set can be modified during each scanning process. This method of hardware and software use does not slow down scanning speed, and it maintains high resolution measurements.
Inherentną cechą piezoskanerów w mikroskopach skanujących jest ich zmienna w czasie nieliniowość. Skutkuje ona zniekształceniami uzyskiwanych obrazów oraz trudnościami prowadzenia pomiarów wymagających adekwatnej kontroli aktualnego położenia cantilevera w stosunku do powierzchni próbki. W artykule przedstawiono metody korekcji nieliniowości piezoskanera. Wskazano zalety i wady poszczególnych rozwiązań oraz opisano opracowaną sprzętowo-programową metodę korekcji. Korekcja realizowana jest przez system cyfrowy złożony z szybkich przetworników analogowo-cyfrowych oraz układu programowalnego FPGA, w którym zaimplementowane są odpowiednie algorytmy. Algorytmy te wyznaczają, na podstawie rzeczywistych danych pomiarowych, zbiór współczynników korekcyjnych dla różnych szybkości i obszarów skanowania. Zbiór ten może być modyfikowany podczas każdego procesu skanowania. Metoda sprzętowo-programowa nie spowalnia szybkości skanowania, przy zachowaniu wysokiej rozdzielczość pomiarów.
Źródło:
Problemy Eksploatacji; 2013, 1; 67-76
1232-9312
Pojawia się w:
Problemy Eksploatacji
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
The Beam Current Considerations in SEM Accordance to Mirror Effect Phenomenon
Autorzy:
Al-Obaidi, H. N.
Khaleel, I. H.
Powiązania:
https://bibliotekanauki.pl/articles/411880.pdf
Data publikacji:
2013
Wydawca:
Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
Tematy:
scanning electron microscope
Electron Beam Current
Electron Mirror Images
Opis:
A theoretical investigation have been presented to exploring the influence of electrons beam current on the electron mirror image deduced inside the scanning electron microscope (SEM). A rough mathematical expression for the electric potential that associated with electron beam is derived. The results clearly shows that the beam current could be used to enhance or conversely deteriorate the phenomena of mirror effect. So this work procedure may consider to be tool controllable of this phenomena for investigation purposes.
Źródło:
International Letters of Chemistry, Physics and Astronomy; 2013, 10, 1; 70-75
2299-3843
Pojawia się w:
International Letters of Chemistry, Physics and Astronomy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
New data on seed coat micromorphology of several Impatiens spp. from Northeast India
Autorzy:
Rewicz, A.
Adamowski, W.
Borah, S.
Gogoi, R.
Powiązania:
https://bibliotekanauki.pl/articles/2130702.pdf
Data publikacji:
2020
Wydawca:
Polskie Towarzystwo Botaniczne
Tematy:
Balsaminaceae
micromorphology
scanning electron microscope (SEM)
seed
surface
taxonomy
Opis:
This study aimed to analyze the seed coat structure of nine species from the genus Impatiens from Northeast India. A review of the available literature showed a scarcity of data on seed sizes and shapes, as well as a complete lack of information on the ultrastructure of seeds from five taxa of Impatiens determined with a scanning electron microscope (SEM). Photographs of the surface structure of seeds from the analyzed species were taken using an SEM and, from these, we measured the length and width of the seeds. The results showed differences in the seed ultrastructure and metric traits within the studied taxa. Based on the ornamentation of the epidermal cells, we distinguished three morphological types: protrusive, granulate, and reticulate. The seeds of the investigated species had ellipsoid, subellipsoid, or subspheroid shapes. Their lengths and widths ranged from 1.2 to 3.6 mm and 0.7 to 2.1 mm, respectively. The results of the study showed that the ultrastructures of Impatiens seeds are different among taxa and for some species can be used as a diagnostic character for their identification.
Źródło:
Acta Societatis Botanicorum Poloniae; 2020, 89, 3
0001-6977
2083-9480
Pojawia się w:
Acta Societatis Botanicorum Poloniae
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Epidermal micromorphology of Hordelymus europaeus (L.) Jess. ex Harz (Poaceae)
Autorzy:
Klimko, M.
Nowinska, R.
Czarna, A.
Powiązania:
https://bibliotekanauki.pl/articles/790567.pdf
Data publikacji:
2015
Wydawca:
Uniwersytet Przyrodniczy w Poznaniu
Tematy:
micromorphology
Hordelymus europaeus
Poaceae
scanning electron microscope
reproductive organ
Opis:
This paper presents the macromorphological and micromorphological characteristics of wood barley recorded in the Wielkopolska Lowland. On the basis of the collection of preserved specimens, the micromorphological features were examined under a scanning electron microscope (SEM) to assess their taxonomic value. The principal features include the size and shape of cork/silica cells, crown cells, prickles, macro-hairs and stomata as well as the morphology of long cells in vegetative and reproductive organs.
Źródło:
Steciana; 2015, 19, 2
1689-653X
Pojawia się w:
Steciana
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Scanning optical and electron microscopes with computer image acquisition
Autorzy:
Konstankiewicz, K.
Pukos, A.
Powiązania:
https://bibliotekanauki.pl/articles/25623.pdf
Data publikacji:
1995
Wydawca:
Polska Akademia Nauk. Instytut Agrofizyki PAN
Tematy:
electron microscope
scanning optical microscope
microscopic method
tandem scanning reflected light microscope
computer image analysis
scanning electron microscopy
biological material
rotary microtome
confocal microscopy
Źródło:
International Agrophysics; 1995, 09, 4
0236-8722
Pojawia się w:
International Agrophysics
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
In-situ Observation of High-Temperature Fracture Behaviour of 347 Stainless Steel Subjected to Simulated Welding Process
Autorzy:
Ko, Seok-Woo
Park, Hyeonwoo
Yoo, Il
Kim, Hansoo
Lee, Joonho
Hwang, Byoungchul
Powiązania:
https://bibliotekanauki.pl/articles/2048885.pdf
Data publikacji:
2021
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
347 stainless steel
in-situ
high temperature
fracture behavior
welding
confocal laser scanning microscope
CLSM
Opis:
In-situ study on the high-temperature fracture behaviour of 347 stainless steel was carried out by using a confocal laser scanning microscope (CLSM). The welding microstructures of the 347 stainless steel were simulated by subjecting the steel specimen to solution and aging treatments. Undissolved NbC carbides were present within grains after solution treatment, and M23C6 carbides were preferentially formed at grain boundaries after subsequent aging treatment. The M23C6 carbides formed at grain boundaries worked as stress concentration sites and thus generated larger cracks during high-temperature tensile testing. In addition, grain boundary embrittlement was found to be a dominant mechanism for the high-temperature fracture of the 347 stainless steel because vacancy diffusion in the Cr-depleted zones enhances intergranular fracture due to the precipitation of M23C6 carbides at grain boundaries.
Źródło:
Archives of Metallurgy and Materials; 2021, 66, 4; 1019-1022
1733-3490
Pojawia się w:
Archives of Metallurgy and Materials
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Calcium (Ca2+) expression and intensity in cumulus-oocyte complex (COCs) in Kacang goat after vitrification
Autorzy:
Widjiati, W.
Faizah, Z.
Darsini, N.
Hendrawan, V.F.
Karima, H.N.
Chotimah, C.
Sumitro, S.B.
Yustinasari, L.R.
Kasman, A.A.M.N.
Ntoruru, J.M.
Luqman, E.M.
Powiązania:
https://bibliotekanauki.pl/articles/16539072.pdf
Data publikacji:
2022
Wydawca:
Polska Akademia Nauk. Czasopisma i Monografie PAN
Tematy:
Calcium (Ca2+)
confocal laser scanning microscope
cumulus-oocyte complex (COCs)
food production
Kacang goat
Opis:
The process of vitrification of the cumulus-oocyte complex (COCs) often results in cold shock. When warming, heat shock occurs which can disrupt the balance of intracellular calcium (Ca2+) intensity. Drastic changes in temperature cause Reactive Oxygen Species (ROS), affecting changes on Ca2+ in COCs. The role of calcium is needed for oocyte activation in the fertilization process. The purpose of this study was to measure the expression of Ca2+ and the intensity of Ca2+ in COCs after vitrification. The study was divided into 2 groups, the control group (C) of fresh COCs, and the treatment group (T) of COCs after vitrification. After vitrification for 24 hours, then thawing, the expression of Ca2+ was examined using the Immunocytochemistry (ICC) method and the intensity of calcium (Ca2+) with a Confocal Laser Scanning Microscope (CLSM). The research data obtained were analyzed statistically by T-Test. The results showed that the expression of Ca2+ in the control group (12.00±0.00) was different from the treatment group (0.35±0.79). The intensity of Ca2+ in the control group (1059.43±489.59) was different from the treatment group (568.21±84.31). The conclusion of this study is that cryopreservation affects calcium in COCs; there were differences in the expression and the intensity of Ca2+ between fresh COCs and COCs after vitrification. Ca2+ intensity of COCs after vitrification was concentrated in the nucleus, while in fresh COCs it was concentrated in the cytoplasm.
Źródło:
Polish Journal of Veterinary Sciences; 2022, 25, 1; 19-26
1505-1773
Pojawia się w:
Polish Journal of Veterinary Sciences
Dostawca treści:
Biblioteka Nauki
Artykuł
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