- Tytuł:
- Community structure of Common carp (Cyprinus carpio Linnaeus, 1758) gut bacteria in the Cirata Reservoir, West Java Province, Indonesia
- Autorzy:
-
Nugrahi, Tri Nazar Ulfi
Mulyani, Yuniar
Agung, M. Untung Kurnia
Iskandar, Iskandar - Powiązania:
- https://bibliotekanauki.pl/articles/1839403.pdf
- Data publikacji:
- 2021
- Wydawca:
- Przedsiębiorstwo Wydawnictw Naukowych Darwin / Scientific Publishing House DARWIN
- Tematy:
-
Next Generation Sequencing
Bacterial community structure
Common carp gut bacteria - Opis:
- Fish are aquatic animals, where they share the same ecosystem with the bacterial community they are associated with. Community structure is a concept that studies the composition of species and their abundance in a community. Information related to bacterial communities associated with their hosts is very important to support fisheries resource management efforts, both through nature conservation and aquaculture. This study of the bacterial community in the digestive tract of fish can be used to support further utilization of potential bacteria that can be used to improve the fish's immune system. This research was conducted to analyze the structure of the bacterial community found in the intestines of Common carp through a metagenomic approach using the NGS (Next-Generation Sequencing) method. The method used is a metagenomic approach, including DNA isolation from Common carp intestines. Then molecular identification was carried out using Illumina's NGS (Next-Generation Sequencing) method using the 16S rRNA gene marker. The results of the analysis using the NGS method showed as many as 19 types of bacterial phyla identified with the top three phyla, namely Fusobacteria (80%), Bacteriodetes (15%), and Firmicutes (3%). The phylum Fusobacteria is dominated by the genera Fusobacterium, Propionigenium, Ilyobacter, Leptotrichia, and Sebaldella. The phylum Bacteriodetes is dominated by the genera Porphyromonas, Odoribacter, Bacteroides, Sphingobacterium, and Barnesiella. The phylum Firmicutes is dominated by the genera Clostridium, Lactococcus, and Sarcina, Bacillus, and Lactobacillus.
- Źródło:
-
World Scientific News; 2021, 161; 130-142
2392-2192 - Pojawia się w:
- World Scientific News
- Dostawca treści:
- Biblioteka Nauki
Artykuł