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    Wyświetlanie 1-7 z 7
    Tytuł:
    Morphological and molecular identification of cyst nematode species (Heterodera spp.) in Algerian cereal fields
    Autorzy:
    Smaha, D.
    Mokrini, F.
    Imren, M.
    Mokabli, A.
    Dababat, A.A.
    Powiązania:
    https://bibliotekanauki.pl/articles/2084684.pdf
    Data publikacji:
    2019
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    cereals
    Heterodera spp.
    cyst nematode
    ITS-rDNA
    wheat
    Opis:
    Cereal cyst nematodes (Heterodera spp.) are distributed globally and cause severe production losses of small grain cereals. To investigate the occurrence of cereal cyst nematodes in wheat-growing areas of Algeria, a survey was conducted and 27 cereal cyst nematode populations were collected. The populations were initially identified based on their morphological and morphometric characters, followed by molecular methods using speciesspecific primers, complemented by ITS-rDNA sequences. The morphological and morphometric features of second-stage juveniles (J2s) and cysts supported the presence of three Heterodera species: H. avenae, H. filipjevi and H. hordecalis. All morphological values of these distinct populations were very similar to those previously described for these species. Using species-specific primers for H. avenae and H. filipjevi, the specific bands of 109 bp and 646 bp confirmed the morphological identification of both species, respectively. In addition, the internal transcribed spacer (ITS) regions were sequenced to study the diversity of the 27 populations. These sequences were compared with those of Heterodera species available in the GenBank database (www.ncbi.nlm.nih.gov) and re-confirmed the identity of the species. Nineteen sequences of ITS-rDNA were similar (99–100%) to the sequences of H. avenae published in the GenBank, six sequences were similar (99–100%) to H. hordecalis, and two were similar (98–99%) to H. filipjevi. The results of this study are of great value to breeding programs and extension services, where they will contribute to the design of control measures to keep damaging nematodes in check.
    Źródło:
    Journal of Plant Protection Research; 2019, 59, 3; 400-411
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data
    Autorzy:
    Zhao, Yan
    Gao, Feng
    Li, Jiqiu
    Yi, Zhenzhen
    Powiązania:
    https://bibliotekanauki.pl/articles/763453.pdf
    Data publikacji:
    2012
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    Phylogeny, Ciliophora, choreotrichs, Tintinnida, SSU rDNA, ITS1-5.8S-ITS2 region, ITS2 secondary structure
    Opis:
    In order to better understand phylogenetic relationships among tintinnid ciliated protozoa, we sequenced and analyzed the SSU rDNA and ITS1-5.8S-ITS2 regions of 10 species belonging to five genera in the order Tintinnida. The secondary structures of the ITS2 region were compared among 8 closely related genera, revealing two stable helices of the palm. In addition, we identified a bulge absence in position II of the ITS2 putative secondary structures of species in basal positions in phylogenetic trees, suggesting the absence bulge might be an ancestral character in the order Tintinnida. Phylogenetic analyses based on SSU rDNA and ITS1-5.8S-ITS2 regions sequence show 1) divergences within the family Tintinnidae are higher than that among other four families (Codonellidae, Ptychocylididae, Metacylididae and Codonellopsidae), suggesting the subdivision of the this family; 2) the family Ptychocylididae is polyphyletic; 3) the subdivision of Tintinnopsis are suggested, because the Tintinnopsis spp. scatter into different clades; 4) species with agglutinated loricae are not clearly separated from that with hyaline ones.
    Źródło:
    Acta Protozoologica; 2012, 51, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Paramecium tredecaurelia: A Unique Non-Polymorphic Species of the P. aurelia spp. Complex (Oligohymenophorea, Ciliophora)
    Autorzy:
    PRZYBOŚ, Ewa
    Tarcz, Sebastian
    Surmacz, Marta
    Sawka, Natalia
    Fokin, Sergei I.
    Powiązania:
    https://bibliotekanauki.pl/articles/763419.pdf
    Data publikacji:
    2013
    Wydawca:
    Uniwersytet Jagielloński. Wydawnictwo Uniwersytetu Jagiellońskiego
    Tematy:
    P. aurelia species complex, intraspecific polymorphism, rDNA (ITS1-5.8S- ITS2-5’LSU rDNA), cytochrome c oxidase subunit I gene (COI), cytochrome b gene (CytB)
    Opis:
    New stands of Paramecium tredecaurelia, a rare species of the P. aurelia spp. complex, were identified in Thailand and Madagascar on the basis of mating reactions and molecular markers (rDNA and mtDNA). Analysis of DNA fragments showed that all P. tredecaurelia strains, the recently recorded ones and the ones known previously from France, Mexico, and Israel, form a monophyletic and well-defined clade in the P. aurelia species trees. All of these strains, collected from different localities around the world, represent identical or nearly identical haplotypes in terms of all the studied DNA fragments. Given the huge distances between particular collection sites, such a low level of variability of the studied sequences may result from a slow rate of evolution in P. tredecaurelia.
    Źródło:
    Acta Protozoologica; 2013, 52, 4
    1689-0027
    Pojawia się w:
    Acta Protozoologica
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Dynamika zbiorowiska grzybów mykoryzowych dębu szypułkowego w warunkach szkółki leśnej
    Temporal dynamics of ectomycorrhizal community of pedunculate oak seedlings during the first year of growth in bare-root forest nursery
    Autorzy:
    Pietras, M.
    Leski, T.
    Rudawska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/989739.pdf
    Data publikacji:
    2015
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    szkolki lesne
    drzewa lesne
    dab szypulkowy
    Quercus robur
    mikoryza
    ektomikoryza
    grzyby mikoryzowe
    grzyby ektomikoryzowe
    zbiorowiska grzybow
    dynamika zbiorowisk
    ectomycorrhiza
    quercus robur
    forest nursery
    its rdna
    Opis:
    Pedunculate oak (Quercus robur L.) is among the major managed tree species in Europe. In Poland, oak woodlands cover 6.9% of forest area. Furthermore, the significance of native oaks for Polish forestry is rising because the Polish National Forest Strategy predicts a growth of forest cover from 28.5% to 33% by year 2050 with the primary focus on the increase in contribution of deciduous tree species (from 22% to 33%). Therefore, for establishment of new stands, oak seedlings are grown for 2−3 years in forest nurseries. For optimal growth and development all European oak species are critically dependent on ectomycorrhizal fungi. The objective of this study was to describe the temporal dynamics of ectomycorrhizal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery. Experiment was conducted in forest nursery Miranowo (western Poland). The species structure of an ectomycorrhizal community was assessed every 10 or 15 days from May till October. Mycorrhizal colonization of tested seedlings changed from 0 to 85% depending of the time of sampling. Based on combination of morphological and molecular techniques, nine ectomycorrhizal fungal taxa were distinguished. The first ectomycorrhizas formed by Scleroderma verrucosum were detected 50 days after acorns sowing. In the next sampling days ectomycorrhizal community was dominated consecutively by Inocybe curvipes and Hebeloma sacchariolens (with maximum of abundance 60.98 and 75.55%, respectively). The obtained results were strongly influenced by the infection with the fungal biotroph Erysiphe alphitoides and resulted in a dramatic reduction in the abundance of living ectomycorrhizas down to 7.5%. Our investigation revealed that ectomycorrhizal fungal community of pedunculate oak seedlings during the first year of growth in bare−root forest nursery is rich in terms of number of species and a temporal changes are very dynamic. Thus we suggest, that in case of pedunculate oak artificial inoculation in general is not necessary for oaks seedlings in nursery practice.
    Źródło:
    Sylwan; 2015, 159, 10; 831-838
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zbiorowiska grzybów ektomykoryzowych modrzewia europejskiego na powierzchni proweniencyjnej w LZD Krynica w Beskidzie Sądeckim
    Ectomycorrhizal fungal communities of European larch from the provenance trial in the Experimental Forestry Unit in Krynica Zdroj (Beskid Sadecki Mountains)
    Autorzy:
    Leski, T.
    Rudawska, M.
    Powiązania:
    https://bibliotekanauki.pl/articles/991059.pdf
    Data publikacji:
    2014
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    LZD Krynica
    Beskid Sadecki
    doswiadczenia proweniencyjne
    powierzchnie doswiadczalne
    drzewa lesne
    modrzew europejski
    Larix decidua
    grzyby ektomikoryzowe
    morfotypy
    identyfikacja
    symbiosis
    ectomycorrhiza
    tree genotype
    larix decidua
    its rdna
    Opis:
    The effects of tree genotype on below−ground ectomycorrhizal fungal communities of 40 years old European larch from Kowary, Bliżyn, Świętokrzyski National Park and Szczytna Śląska origins was investigated. The research was carried out in the provenance trial in the Experimental Forestry Unit in Krynica Zdrój (Beskid Sądecki Mountains, S Poland). Ectomycorrhizal fungal communities were assessed by a combination of morphological and molecular techniques. In total 22 fungal taxa has been distinguished (from 17 to 20, depending on larch provenance). Thirteen ectomycorrhizal fungal taxa were common to all analysed origins. The mean taxa richness, Shannon diversity and Simpson dominance coefficient did not differ significantly between provenances. Based on fungal taxa composition (Jaccard coefficient) tested larch origins were characterized by a high level of similarity of ectomycorrhizal fungal communities. Six detected fungal taxa have not been previously reported as symbiotic partners of European larch.
    Źródło:
    Sylwan; 2014, 158, 05; 352-360
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Zastosowanie regionu ITS1/2 rDNA i 18S rDNA do badania mykobioty gleby leśnej
    Use of ITS1/2 rDNA and 18S rDNA in studies of the forest soil mycobiota
    Autorzy:
    Behnke-Borowczyk, J.
    Kwaśna, H.
    Powiązania:
    https://bibliotekanauki.pl/articles/989532.pdf
    Data publikacji:
    2016
    Wydawca:
    Polskie Towarzystwo Leśne
    Tematy:
    lesnictwo
    gleby lesne
    mikroorganizmy glebowe
    grzyby glebowe
    struktura zbiorowisk
    metody badan
    metody molekularne
    DNA rybosomalny
    region ITS1/2
    region 18S
    detection
    forest
    its1/2 rdna
    ns1
    ns2
    18s rdna
    microorganisms
    mycobiota
    soil
    Opis:
    The aim of the studies was to check the usefulness of ITS1/2 rDNA and 18S rDNA regions in the molecular investigation of forest soil microbiota structure. Soil studied, originated from a 1−year−old plantation and a 40−year old stand of Scots pine located in Bierzwnik and Międzychów forest districts located 200 km apart. The hypothesis assumed that both approaches lead to the discovery of abundant microbiota communities with different structures and with rare common species. The environmental DNA was extracted with a Power Soil ® DNA Isolation Kit from two soil samples in each site. The ITS1/2 rDNA was amplified with specific primers ITS1 and ewfitsrev 1, and 18S rDNA with universal primers NS1 and NS2. PCR products were cloned into pGEM−T Easy. Inserts were primarily selected in blue/white screening on a X−gal medium. Representative clones were further selected in two separate RFLP analyses with HhaI and BsuRI restriction enzymes. Representative clones purified and sequenced using the Sanger Method in the DNA Research Centre (Poznań). Each sequence was identified to the lowest taxonomic rank. Ninety to 233 clones with DNA of 5−44 taxa including 3−37 taxa of fungi were obtained from 4 samples of soil. After application of ITS1/2 rDNA and 18S rDNA, the fungal DNA was detected respectively in 89,60−100,00% and 11,77−64,8% clones and the number of fungal species detected was respectively 12−37 and 3−19. Fungi were represented by four orders: Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota. Both primers also amplified also DNA of other organisms (mostly from Animalia and Protista Kingdom) represented by 0−9 taxa. If compared, the application of forest soil microbiota structure with ITS1/2 rDNA and 18S rDNA led to detect a lower abundance of fungi and a bigger abundance of other organisms. Considering the higher number of clones and taxa recognized, the region of ITS1/2 rDNA was more effective in the studies of the soil microbiota structure. The region of 18S rDNA was efficient in local detection of Chytridiomycota and Zygomycota and of rare species of fungi from Ascomycota and Basidiomycota. Despite the deficiency of NCBI database the use of the 18S rDNA region in studies on fungal community the region should be included in molecular studies of fungal diversity. It is concluded that studies on the biodiversity of soil microorganisms need the application of a few independent methods of detection and identification.
    Źródło:
    Sylwan; 2016, 160, 07; 564-572
    0039-7660
    Pojawia się w:
    Sylwan
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
    Tytuł:
    Occurrence of Ditylenchus destructor Thorne, 1945 on a sand dune of the Baltic Sea
    Autorzy:
    Dobosz, R.
    Rybaczyk-Mydlowska, K.
    Winiszewska, G.
    Powiązania:
    https://bibliotekanauki.pl/articles/2084759.pdf
    Data publikacji:
    2020
    Wydawca:
    Polska Akademia Nauk. Czytelnia Czasopism PAN
    Tematy:
    Ammophila arenaria
    internal transcribed spacer (ITS)
    potato rot nematode
    18S
    28S rDNA
    Opis:
    Ditylenchus destructor is a serious pest of numerous economically important plants worldwide. The population of this nematode species was isolated from the root zone of Ammophila arenaria on a Baltic Sea sand dune. This population’s morphological and morphometrical characteristics corresponded to D. destructor data provided so far, except for the stylet knobs’ height (2.1–2.9 vs 1.3–1.8) and their arrangement (laterally vs slightly posteriorly sloping), the length of a hyaline part on the tail end (0.8–1.8 vs 1–2.9), the pharyngeal gland arrangement in relation to the intestine (dorsal or ventral vs dorsal, ventral or lateral) and the appearance of vulval lips (smooth vs annulated). Ribosomal DNA sequence analysis confirmed the identity of D. destructor from a coastal dune.
    Źródło:
    Journal of Plant Protection Research; 2020, 60, 1; 31-40
    1427-4345
    Pojawia się w:
    Journal of Plant Protection Research
    Dostawca treści:
    Biblioteka Nauki
    Artykuł
      Wyświetlanie 1-7 z 7

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