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Wyświetlanie 1-11 z 11
Tytuł:
A breakthrough in the efficiency of contact DNA insecticides: rapid high mortality rates in the sap-sucking insects Dynaspidiotus britannicus Comstock and Unaspis euonymi Newstead
Autorzy:
Gal`chinsky, N.
Useinov, R.
Yatskova, E.
Laikova, K.
Novikov, I.
Gorlov, M.
Trikoz, N.
Sharmagiy, A.
Plugatar, Y.
Oberemok, V.
Powiązania:
https://bibliotekanauki.pl/articles/2082776.pdf
Data publikacji:
2020
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
insect pest control
sap-sucking
insects
28S ribosomal RNA
Opis:
In this short communication describing experiments carried out on the larvae of two insects, Unaspis euonymi Comstock (feeding on Euonymus japonicus Thunb.) and Dynaspidiotus britannicus Newstead (feeding on Laurus nobilis L.), we evaluate for the first time the efficiency of using DNA insecticides in the control of sap-sucking insects, including armored scale insects. Over a period of 10 days, high insect mortality was detected in both U. euonymi and D. britannicus, accompanied by a significant decrease in the concentration of target RNAs. At the same time, no visible changes were observed when the leaves of the host plants were subjected to treatment with DNA insecticides for one month. The results show the high efficiency of DNA insecticides used against hemipteran insect pests. It is noteworthy that the high efficiency of DNA insecticides and their low cost in comparison with RNA preparations provides a safe and extremely promising potential vehicle for the control of sap-sucking insects.
Źródło:
Journal of Plant Protection Research; 2020, 60, 2; 220-223
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
Autorzy:
Oberemok, V.V.
Laikova, K.V.
Useinov, R.Z.
Gal`chinsky, N.V.
Novikov, I.A.
Yurchenko, K.A.
Volkov, M.E.
Gorlov, M.V.
Brailko, V.A.
Plugatar
Powiązania:
https://bibliotekanauki.pl/articles/2084849.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
gypsy moth
insect pest control
Lymantria dispar
5.8S ribosomal RNA
Opis:
5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 561-564
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Larvicidal activity of Bacillus thuringiensis Colombian native strains against Bemisia tabaci (Hemiptera: Aleyrodidae)
Autorzy:
Cabra, E.T.
Hernandez Fernandez, J.A.
Powiązania:
https://bibliotekanauki.pl/articles/2084862.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
biological control
genes cry
insect pest
molecular characterization
Opis:
The whitefly, Bemisia tabaci, an insect of the order Hemiptera which attacks more than 600 species of plants, is one of the most important agricultural pests around the world. The insecticidal Cry proteins from Bacillus thuringiensis (Bt) are useful biological pesticides, and some are toxic to Hemipteran insects. In this study, Colombian native isolates of Bt were functionally characterized at molecular and biological levels. The strains contained between one and five different crystal shapes: round, triangular, amorphous, bipyramidal and squared. The strains presented between three to seven bands of proteins in their electrophoretic pattern that were organized into six groups according to their possible biological activity on insect pests. Cry1Aa, cry1Ab, cry1Ac, cry1B and cry1C genes were identified for PCR in the different Bt isolates. Bioassays were performed on tomato leaves whose surface was spread with 3 µg · ml−1 crude extract of Bt toxins. Second instar larvae of whitefly, which were placed on top of leaves and exposed to the toxins for 7 days, exhibited mortalities from 18 to 69%. The lethal concentration 50 (LC50) of ZBUJTL39, Bt kurstaki HD1 and ZCUJTL9 strains were 1.83, 1.85 and 2.16 μg · ml−1, respectively (p < 0.05). These results show that the native Bt strain ZBUJTL39, which contained the genes cry1Aa, cry1Ab, cryCa and cryBa could eventually be used for the development of an integrated management program together with other tools for the control of B. tabaci.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 503-511
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insecticidal efficacy of silica nanoparticles against Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val
Autorzy:
Ziaee, M.
Ganji, Z.
Powiązania:
https://bibliotekanauki.pl/articles/66106.pdf
Data publikacji:
2016
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insecticidal potential
silica
nanoparticle
Rhyzopertha dominica
Tribolium confusum
barley
pest control
plant protection
wheat
insect pest
Opis:
Bioassays were conducted to assess the effects of two silicon dioxide nanoparticles of Aerosil® and Nanosav against adults of Rhyzopertha dominica F. and Tribolium confusum Jacquelin du Val. Silica nanoparticles were applied at the rates of 50, 100, 200 and 300 mg · kg–1 on wheat and peeled barley. The mortality was counted after 1, 2, 3, and 7 days of exposure. Another experiment was carried out to evaluate the effect of food source on the survival of beetles after exposure to silica nanoparticles. Adults were exposed to silica nanoparticles at the rate of 0.2 mg · cm–2 for 1 and 2 days on filter paper inside plastic Petri dishes, respectively. After exposure, the initial mortality was counted and live individuals of both species were held for a week in empty glass vials or vials containing wheat and wheat flour, respectively. Silica nanoparticles have high toxicity on R. dominica and T. confusum adults. Rhyzopertha dominica was more susceptible than T. confusum. However, the mortality of both species increased with increasing concentrations and time exposed to each concentration. At low concentrations, Aerosil® was more effective than Nanosav. Silica nanoparticles were more effective in wheat grains than barley. Results indicated that the initial mortality was so high that the impact of food source on delay mortality was unclear in most cases. Silica nanoparticles were efficient against tested species and can be used effectively in a stored grain integrated pest management program.
Źródło:
Journal of Plant Protection Research; 2016, 56, 3
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biogenic silver nanoparticles from Trichodesma indicum aqueous leaf extract against Mythimna separata and evaluation of its larvicidal efficacy
Autorzy:
Buhroo, A.A.
Nisa, G.
Asrafuzzaman, S.
Prasad, R.
Rasheed, R.
Bhattacharyya, A.
Powiązania:
https://bibliotekanauki.pl/articles/65016.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
insect
pest control
biogenic substance
silver nanoparticle
Trichodesma indicum
water extract
leaf extract
Mythimna separata
Oriental armyworm
pest
larvicidal activity
Opis:
Th e present exploration is focused on the bio-fabrication of silver nanoparticles (Ag NPs) using Trichodesma indicum aqueous leaf extract as a reducing agent. Th e synthesized Ag NPs were productively characterized by UV-vis spectroscopy, XRD, and TEM studies. Th e photosynthesis of Ag NPs was done at room temperature for 24 h and at 60°C. Th e green synthesis of spherical-shaped Ag NPs bio-fabricated from T. indicum with a face centred cubic structure showed average particle sizes of 20–50 nm, which is inconsistent with the particle size calculated by the XRD Scherer equation and TEM analysis. We further explored the larvicidal effi cacy of biosynthesized Ag NPs with leaf extracts of T. indicum against Mythimna separata. Th e results showed that Ag NPs (20–50 nm) of T. indicum possess good larvicidal activity against M. separata with an LC50 of 500 ppm. Th us, we can advocate that Ag NPs of 20–50 nm size extracted from T. indicum may be considered in the pest management programme of M. separata in future.
Źródło:
Journal of Plant Protection Research; 2017, 57, 2
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
System for warning and control of leaf mining flies and leaf beetles on cereal crops
Autorzy:
Walczak, F
Powiązania:
https://bibliotekanauki.pl/articles/65294.pdf
Data publikacji:
1998
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
pest
Agromyzidae
crop damage
control
warning
larva
leaf mining fly
cereal crop
trapped insect
Opis:
Many species of leaf mining flies feed on cereal crops. Cereal crop plantations with high level of the pest infestation need to be treated with insecticides. The yellow tables covered with glue were used for trapping the pests. The leaf mining flies warning system can provide the information about date of treatment based on counting the trapped imagines of the pest and observation of leaf beetle appearance and its development. The following species of leaf mining flies are the most common in Poland: Phytomyza nigra Mg., Agromyza ambigua Fll., Ceroclontha pygmaea Mg., Agromyza mobilis Mg.
Wzrost liczebności i szkodliwości miniarek w uprawach zbóż w Polsce stwierdzono na początku lat dziewięćdziesiątych. Larwy miniarek żerują w tkance miękiszowej liści, w wyniku czego powstają charakterystyczne uszkodzenia (miny). W niektórych rejonach kraju lokalnie owady te mogą występować liczniej i przyczyniać się do znacznego ograniczenia powierzchni asymilacyjnej liści (głównie flagowych i podflagowych), co w konsekwencji ma wpływ na wartość i wielkość plonu. Celem pracy było opracowanie wskazówek metodycznych przy podejmowaniu decyzji o chemicznym zwalczaniu na zbożach miniarek równocześnie ze skrzypionkami. W wyniku przeprowadzonych badań stwierdzono, że dobrą praktyczną metodą sygnalizacji chemicznego zwalczania tych szkodników jest kontrola liczebności form dorosłych miniarek, odławianych na tablicach chwytnych w kolorze żółtym, przy równoległej obserwacji pojawu i rozwoju skrzypionek.
Źródło:
Journal of Plant Protection Research; 1998, 38, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Development of thermotolerant isolates of Beauveria bassiana (Bals.-Criv.) Vuill. with ethyl methanesulfonate
Autorzy:
Wongwanich, Y.
Cobelli, P.
Boonchuay, D.
Wangsomboondee, T.
Powiązania:
https://bibliotekanauki.pl/articles/65516.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
thermotolerance
entomopathogenic fungi
Beauveria bassiana
ethyl methanesulphonate
brown planthopper
Nilaparvata lugens
pest insect
pest control
mutation
fungal isolate
molecular identification
pathogenicity test
Opis:
Beauveria bassiana is an entomopathogenic fungus that is widely used in Thailand to control pest insects. However, the increasing temperature has influenced the insect control efficiency of the fungus. Therefore, determination of thermotolerant isolates of B. bassiana that can grow and remain pathogenic at higher temperatures than its current optimum temperature may be a better way to control pest insects in a high temperature environment. Three isolates of B. bassiana obtained from the Rice Department, Thailand were selected for mutagenesis using ethyl methanesulfonate (EMS) with subsequent screening at high temperatures (33 and 35°C). In addition, the recovery of fungal growth after exposure to a high temperature for a period of time (5–15 days) and then transferring to 25°C was evaluated. No isolates were found that grew at 35°C but one mutant isolate (BCNT002MT) produced larger diameter colonies and more spores than the corresponding wild type (WT) at 33°C. Growth and spore production of the BCNT002MT isolate were greater than its WT when incubated at 25°C for 14 days following exposure to 33°C for 7 days. In addition, the spore germination level (%) of BCNT002MT was significantly higher than its WT during culture at 25°C after prior exposure to 33°C for 5, 10 and 15 days. The pathogenicity against the brown planthopper, Nilaparvata lugens (Stål), of this mutant isolate was also prominent.
Źródło:
Journal of Plant Protection Research; 2017, 57, 4
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Toxicological and environmental aspects of carrot fly [Psila rosae Fabr.] control
Autorzy:
Sadlo, S.
Filar, M.
Szpyrka, E.
Powiązania:
https://bibliotekanauki.pl/articles/55198.pdf
Data publikacji:
2008
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
insecticide
environment
carrot fly
residue
parsley
chlorothalonil
fungicide
toxicology
plantation
pest control
insect
plant protection
Psila rosae
chlorpyrifos
carrot
Opis:
The objectives of this work were to describe the residual behaviour of chlorpyrifos (insecticide) and chlorothalonil (fungicide), applied for the protection of carrot and parsley plantations. The field tests were carried out at farms located near Rzeszów (South-Eastern Poland) on processing carrot and parsley destined for baby food production. The results obtained indicated that disappearance rate of chlorothalonil on carrot leaves was significantly faster and its half-life time was about 7 times shorter than that of chlorpyrifos. In weather conditions of 2006, chlorpyrifos residues in rape carrot and parsley roots were reduced to levels lower than MRL and even the rigorous value of 0.01 mg/kg.
Źródło:
Pestycydy; 2008, 1-2; 119-126
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Badania laboratoryjne przydatnosci biopreparatu Dipel w redukcji prusakow [Blattella germanica L.]
Laboratory studies of the usefulness of the biopreparation Dipel for redukction of the population of german cocroaches [Blattella germanica L.]
Autorzy:
Zukowski, K
Powiązania:
https://bibliotekanauki.pl/articles/873190.pdf
Data publikacji:
1993
Wydawca:
Narodowy Instytut Zdrowia Publicznego. Państwowy Zakład Higieny
Tematy:
ochrona srodowiska
zwalczanie szkodnikow
owady
biopreparaty
badania laboratoryjne
szkodniki zywnosci
prusak
Blattella germanica
Dipel
szkodniki magazynowe
karaczany
environment protection
pest control
insect
biopreparation
laboratory research
food pest
German cockroach
Blatella germanica
Dipel biopreparation
storage pest
cockroach
Opis:
Określono wrażliwość prusaków (Blattella germanica L.) różnorodnych pod względem wieku i płci, na owadobójczy biopreparat z Bacillus thuringiensis var. kurs taki (Dipel).
In the studies conducted for finding of biological factors effectively controlling insects of hygienic importance German cockroaches were subjected to the action of an insecticide containing spores of Bacillus thuringiensis var. kurstaki. One gram of the insecticide contained 25 miliard (US billion) spores with protein endotoxin crystals. The inscets were given drinking water and food containing 1.0%, 0.5%, 0.25%, 0.125% of the initial preparation. The experiment was carried out on 1800 cockroaches. Half of them (600 mature insects differentiated for sex and age and 300 larvae in stage 1st of development) were treated with the preparation, the remaining insects served for control. The insects were kept at about 28°C. After 9 days o the experiment in the group receiving 1% Dipel on food 76.4% mature insects and 83,0% larvae in 1st stage of development died. After 14 days all males died, and after 17 days died all females. In the control group died in that time 2.3% and 3.4% respectively. In case of lower per cent of the preparation in food the time of dying was prolonged, and at the lowest per cent it was 22 days. The study showed that Dipel effectively reduced the population of German cockroaches, and although in initial period its action was slower than that of chemical agents, it could be used for the control of these insects, especially as it in non-toxic for humans, warm-blooded animals and useful insects.
Źródło:
Roczniki Państwowego Zakładu Higieny; 1993, 44, 2-3; 227-230
0035-7715
Pojawia się w:
Roczniki Państwowego Zakładu Higieny
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Możliwości wykorzystania Mamestra dissimilis Knoch. do regulacji zachwaszczenia przez Rumex confertus Willd.
Mamestra dissimilis Knoch. potential to control weed infestation with mossy sorrel (Rumex confertus Willd.)
Autorzy:
Piesik, D.
Wenda-Piesik, A.
Powiązania:
https://bibliotekanauki.pl/articles/47114.pdf
Data publikacji:
2005
Wydawca:
Politechnika Bydgoska im. Jana i Jędrzeja Śniadeckich. Wydawnictwo PB
Tematy:
Mamestra dissimilis
pietnowka zmienna
szkodniki roslin
zerowanie
Rumex confertus
szczaw omszony
chwasty
regulacja zachwaszczenia
biologiczne metody ochrony roslin
plant pest
insect foraging
weed
weed infestation control
biological plant protection
Opis:
Badania polowe i laboratoryjne prowadzono w celu oszacowania możliwości wykorzystania piętnówki zmiennej (Mamestra dissimilis Knoch., Lepidoptera: Noctuidae) do regulacji zachwaszczenia spowodowanego przez Rumex confertus (Willd.). W naturalnym siedlisku szczawiu badano dynamikę jego wzrostu oraz liczbę gąsienic piętnówki zmiennej zasiedlających roślinę w okresie 50 dni. Największą liczbę gąsienic odłowiono pod koniec pierwszej dekady czerwca, w czasie gdy roślina żywicielska miała już w pełni wykształconą rozetę. W warunkach laboratoryjnych badano wzrost masy ciała gąsienic oraz ich żerowanie w temperaturze 20oC. Zależności pomiędzy masą zjedzonych liści szczawiu omszonego a przyrostami masy larw L4 i L5 były statystycznie istotne (r = 0,97 i r = 0,88). Stwierdzono bardzo progresywny charakter żerowania larw L4, opisany równaniem 1. stopnia (r = 0,98). Wyliczono, że każdego dnia w ciągu 20 dni żerowania larwy zjadły o 5,9 mg więcej liści. Natomiast dla gąsienic L5 związek ten miał charakter funkcji 2. stopnia (r = 0,96). Oszacowana masa liści szczawiu omszonego, która uległa skonsumowaniu przez larwy piętnówki zmiennej w warunkach naturalnych, wyniosła 159,3 g, co stanowiło około 11% utraty biomasy żywiciela.
Field and laboratory research were conducted to determine the potential of Mamestra dissimilis Knoch., Lepidoptera: Noctuidae to control mossy sorrel (Rumex confertus Willd.). In the natural sorrel habitat a dynamics of the plant growth and the number of Mamestra dissimilis Knoch larvae occurring on that plant over 50 days were researched. The highest number of M. dissimilis larvae was caught at the end of the first decade of June when the host plant rosette was fully developed. In the laboratory, the weight of consumed food by larvae, and larval body weight were measured at 20oC. The relations between the weight of the mossy sorrel leaves consumed and L4 and L5 larvae body weight gains were significant, (r = 0.97 and r = 0.88, respectively). A very progressive feeding of L4 larvae was noted, which was defined by first degree equation (r = 0.98). It was calculated that each day over 20 days of feeding L4 larvae consumed about 5.9 mg more leaves than L5 larvae. For L5 larvae, the relationship was the second degree equation (r = 0.96). An estimated weight of mossy sorrel leaves consumed by M. dissimilis larvae under natural conditions was 159.3 g, which accounted for about 11% loss of the host biomass.
Źródło:
Acta Scientiarum Polonorum. Agricultura; 2005, 04, 1
1644-0625
Pojawia się w:
Acta Scientiarum Polonorum. Agricultura
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Biorational control of arthropod pests with emphasis on the use of the chitin synthesis inhibitor novaluron
Autorzy:
Ishaaya, I.
Lebedev, G.
Ghanim, M.
Horowitz, A.R.
Powiązania:
https://bibliotekanauki.pl/articles/55215.pdf
Data publikacji:
2011
Wydawca:
Sieć Badawcza Łukasiewicz - Instytut Przemysłu Organicznego
Tematy:
biorational control
arthropod
pest
chitin synthesis
novaluron
insecticide
insect
growth regulator
buprofezin
diflubenzuron
chlorfluazuron
pyriproxyfen
tebufenozide
methoxyphenozide
neonicotinoid
imidacloprid
acetamiprid
thiamethoxam
glutamate receptor
gamma-aminobutyric acid
abamectin
Spinosad preparation
novel insecticide
Opis:
Efforts have been made during the past three decades to develop insecticides with selective properties that act specifically on biochemical sites present in a particular insect group, but whose properties differ from those present in mammals. This approach has led to the discovery of compounds that affect the hormonal regulation of molting and developmental processes in insects such as ecdysone agonists, juvenile hormone (JH) mimics and chitin synthesis inhibitors. The search for potent acylureas has led to the development of novaluron (Rimon) developed by Makhteshim Chemical Works. The LC-50 value of novaluron on 3rd-instar Spodoptera littoralis fed on treated leaves is approximately 0.1 mg a.i./liter. This value resembles that of chlorfluazuron and is tenfold lower than that of teflubenzuron. Novaluron affects nymphs of Bemisia tabaci more than chlorfluazuron and teflubenzuron. Artificial rain, at a rate of 40 mm/h applied 5 and 24 h after treatment in a cotton field had no appreciable effect on the potency of novaluron on both S. littoralis larvae and B. tabaci nymphs. Hence, novaluron can be used in tropical areas and during rainy seasons. In general, benzoylphenyl ureas had no direct effect on parasitoids and phytoseiids and are considered mildly affect other natural enemies. Novaluron has no cross-resistance with conventional insecticides, the JH mimics pyriproxyfen and neonicotinoids. As such, it is considered an important compound in pest management programs.
Źródło:
Pestycydy; 2011, 1-4
0208-8703
Pojawia się w:
Pestycydy
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-11 z 11

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