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Wyszukujesz frazę "gypsy moth" wg kryterium: Temat


Wyświetlanie 1-3 z 3
Tytuł:
Topical treatment of LdMNPV-infected gypsy moth caterpillars with 18 nucleotides long antisense fragment from LdMNPV IAP3 gene triggers higher levels of apoptosis in infected cells and mortality of the pest
Autorzy:
Oberemok, Volodymyr V.
Laikova, Kateryna V.
Zaitsev, Alexey S.
Nyadar, Palmah M.
Gninenko, Yuri I.
Gushchin, Vladimir A.
Makarov, Valentin V.
Agranovsky, Alexey A.
Powiązania:
https://bibliotekanauki.pl/articles/961638.pdf
Data publikacji:
2017
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
baculoviral infection
dna insecticides
dna oligonucleotides
forest and
crop protection
gypsy moth
iap genes
Opis:
The high efficiency of baculovirus infection is partially explained by the ability of the virus to suppress host defense machinery connected with the apoptosis pathway. Members of the baculovirus gene family, inhibitors of apoptosis (vIAPs), have been shown to inhibit apoptosis in baculovirus-infected cells. Here we showed that treatment of the LdMNPV- -infected 1st instar gypsy moth (Lymantria dispar) caterpillars with sense (oligoBIR) and antisense (oligoRING) DNA oligonucleotides from the LdMNPV IAP3 gene induced elevated mortality of the insects. Apoptotic DNA ladder assay showed that the leading role in this phenomenon is played by the antisense oligoRING fragment of the vIAP3 gene. These results imply that the application of both antisense DNA oligonucleotides from vIAP genes and baculovirus preparations (one following the other) may be a potential method for plant protection against insect pests.
Źródło:
Journal of Plant Protection Research; 2017, 57, 1
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Insecticidal activity of three 10–12 nucleotides long antisense sequences from 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. against its larvae
Autorzy:
Oberemok, V.V.
Laikova, K.V.
Useinov, R.Z.
Gal`chinsky, N.V.
Novikov, I.A.
Yurchenko, K.A.
Volkov, M.E.
Gorlov, M.V.
Brailko, V.A.
Plugatar
Powiązania:
https://bibliotekanauki.pl/articles/2084849.pdf
Data publikacji:
2019
Wydawca:
Polska Akademia Nauk. Czytelnia Czasopism PAN
Tematy:
antisense oligonucleotides
DNA insecticides
gypsy moth
insect pest control
Lymantria dispar
5.8S ribosomal RNA
Opis:
5.8S ribosomal RNA plays an important role in protein synthesis and eukaryotic ribosome translocation. Contact DNA insecticides based on antisense fragments of 5.8S ribosomal RNA gene of gypsy moth Lymantria dispar L. showed prospective insecticidal activity on its larvae. The most pronounced insecticidal effect was found for antisense fragments 10 and 11 nucleotides long (oligoRIBO-10 and oligoRIBO-11), whereas 12 nucleotides long fragment (oligoRIBO-12) caused the lowest level of insect mortality. This data corresponds to results obtained earlier using rabbit reticulocyte and wheat germ extracts, where maximum inhibition of protein synthesis was observed when a relevant oligomer 10-11 nucleotides long was used, whilst longer chain lengths resulted in reduced inhibition. Using oligoRIBO-11 fragment we have shown penetration of antisense oligonucleotides to insect cells through insects’ exoskeletons. MALDI technique registered the penetration of the oligoRIBO-11 fragment into insect cells after 30 min and a significant response of insect cells to the applied oligonucleotide after 60 min, which indicates not only that the oligonucleotide enters the insect cells, but also the synthesis of new substances in response to the applied DNA fragment. Contact DNA insecticides developed from the L. dispar 5.8S ribosomal RNA gene provide a novel biotechnology for plant protection using unmodified antisense oligonucleotides.
Źródło:
Journal of Plant Protection Research; 2019, 59, 4; 561-564
1427-4345
Pojawia się w:
Journal of Plant Protection Research
Dostawca treści:
Biblioteka Nauki
Artykuł
Tytuł:
Temperature effects performance of Lymantria dispar larvae feeding on leaves of Quercus robur
Autorzy:
Karolewski, P
Grzebyta, J.
Oleksyn, J.
Giertych, M.J.
Powiązania:
https://bibliotekanauki.pl/articles/41435.pdf
Data publikacji:
2007
Wydawca:
Polska Akademia Nauk. Instytut Dendrologii PAN
Tematy:
herbivore insect
sex ratio
Lymantria dispar
larva
gypsy moth
feeding
leaf
oak
English oak
Quercus robur
pedunculate oak
temperature
performance
Opis:
Future climatic warming may modifyinsect development, sex ratio, quantitative changes in populations that could affect the frequency of outbreaks. Here we analyzed the influence of temperature on larval growth and development in the gypsy moth (Lymantria dispar L.). The larvae were reared at three constant temperatures: 15, 20 or 25°C, and fed with leaves of the English oak (Quercus robur L.). Larval mortality, duration of development (DD), relative growth rate (RGR), total mass of food eaten (TFE), and pupal mass (PM) were estimated. Larval mortality was lowest at 20°C, higher at 25°C, and highest at 15°C. DD significantly decreased with increasing temperature and depended on sex. The influence of temperature on the shortening of DD was stronger in males than in females. RGR significantly depended on temperature and was the highest at 25°C, and lowest at 15°C. At 15°C, RGR did not change markedly with time. In contrast, RGR at 20°C was characterized bya continuous decreasing trend. At 25°C, RGR was very high for 2 weeks but quickly declined afterwards. Temperature did not affect the TFE. PM was significantly correlated with temperature and sex. PM of females was higher at 20°C than at 15 and 25°C, in contrast to that of males, which was similar at 20 and 25°C, and higher than at 15°C. For larval growth and development, the most favourable was the medium temperature (20°C). The least favourable temperature for females was 25°C, for males 15°C. The results suggest that global warming may modifythe future sex ratio of gypsy moths that may affect insect development and outbreaks.
Źródło:
Dendrobiology; 2007, 58; 43-49
1641-1307
Pojawia się w:
Dendrobiology
Dostawca treści:
Biblioteka Nauki
Artykuł
    Wyświetlanie 1-3 z 3

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